Profiling Living Bacteria Informs Preparation of Fecal Microbiota Transplantations
Publication Date
January 26, 2017
Journal
PLOS ONE
Authors
Nathaniel D. Chu, Mark B. Smith, Allison R. Perrotta, Zain Kassam, et al
Volume
12
Issue
1
Pages
e0170922
DOI
https://dx.plos.org/10.1371/journal.pone.0170922
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0170922
Scopus
85010952947
Mendeley
http://www.mendeley.com/research/profiling-living-bacteria-informs-preparation-fecal-microbiota-transplantations
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Mendeley | Further Information

{"title"=>"Profiling living bacteria informs preparation of fecal microbiota transplantations", "type"=>"journal", "authors"=>[{"first_name"=>"Nathaniel D.", "last_name"=>"Chu", "scopus_author_id"=>"57193123343"}, {"first_name"=>"Mark B.", "last_name"=>"Smith", "scopus_author_id"=>"56041486600"}, {"first_name"=>"Allison R.", "last_name"=>"Perrotta", "scopus_author_id"=>"55790507400"}, {"first_name"=>"Zain", "last_name"=>"Kassam", "scopus_author_id"=>"54916516700"}, {"first_name"=>"Eric J.", "last_name"=>"Alm", "scopus_author_id"=>"6603687290"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-85010952947", "pmid"=>"28125667", "doi"=>"10.1371/journal.pone.0170922", "pui"=>"614218607", "isbn"=>"1111111111", "sgr"=>"85010952947"}, "id"=>"a1db910b-dd94-39fc-8339-6ccf12a9082e", "abstract"=>"Fecal microbiota transplantation is a compelling treatment for recurrent Clostridium difficile infections, with potential applications against other diseases associated with changes in gut microbiota. But variability in fecal bacterial communities—believed to be the therapeutic agent—can complicate or undermine treatment efficacy. To understand the effects of transplant preparation methods on living fecal microbial communities, we applied a DNA-sequencing method (PMA-seq) that uses propidium monoazide (PMA) to differentiate between living and dead fecal microbes, and we created an analysis pipeline to identify individual bacteria that change in abundance between samples. We found that oxygen exposure degraded fecal bacterial communities, whereas freeze-thaw cycles and lag time between donor defecation and transplant preparation had much smaller effects. Notably, the abundance of Faecalibacterium prausnitzii—an anti-inflammatory commensal bacterium whose absence is linked to inflammatory bowel disease—decreased with oxygen exposure. Our results indicate that some current practices for preparing microbiota transplant material adversely affect living fecal microbial content and highlight PMA-seq as a valuable tool to inform best practices and evaluate the suitability of clinical fecal material.", "link"=>"http://www.mendeley.com/research/profiling-living-bacteria-informs-preparation-fecal-microbiota-transplantations", "reader_count"=>47, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>2, "Researcher"=>6, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>2, "Other"=>9, "Student > Master"=>5, "Student > Bachelor"=>4, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>2, "Researcher"=>6, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>2, "Other"=>9, "Student > Master"=>5, "Student > Bachelor"=>4, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>5, "Engineering"=>2, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>7, "Agricultural and Biological Sciences"=>15, "Medicine and Dentistry"=>12, "Veterinary Science and Veterinary Medicine"=>1, "Physics and Astronomy"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>12}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>15}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}, "Unspecified"=>{"Unspecified"=>5}, "Environmental Science"=>{"Environmental Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Netherlands"=>1, "United States"=>1}, "group_count"=>4}

Scopus | Further Information

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