Effective antibodies immobilization and functionalized nanoparticles in a quartz-crystal microbalance-based immunosensor for the detection of parathion
Publication Date
February 09, 2017
Authors
Bartolomeo Della Ventura, Marco Iannaccone, Riccardo Funari, Massimo Pica Ciamarra, et al
Volume
12
Issue
2
Pages
e0171754
DOI
https://dx.plos.org/10.1371/journal.pone.0171754
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0171754
Scopus
85012050639
Mendeley
http://www.mendeley.com/research/effective-antibodies-immobilization-functionalized-nanoparticles-quartzcrystal-microbalancebased-imm
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Mendeley | Further Information

{"title"=>"Effective antibodies immobilization and functionalized nanoparticles in a quartzcrystal microbalance-based immunosensor for the detection of parathion", "type"=>"journal", "authors"=>[{"first_name"=>"Bartolomeo Della", "last_name"=>"Ventura", "scopus_author_id"=>"57196673843"}, {"first_name"=>"Marco", "last_name"=>"Iannaccone", "scopus_author_id"=>"8938761100"}, {"first_name"=>"Riccardo", "last_name"=>"Funari", "scopus_author_id"=>"55789939700"}, {"first_name"=>"Massimo Pica", "last_name"=>"Ciamarra", "scopus_author_id"=>"6507339454"}, {"first_name"=>"Carlo", "last_name"=>"Altucci", "scopus_author_id"=>"7003541368"}, {"first_name"=>"Rosanna", "last_name"=>"Capparelli", "scopus_author_id"=>"55906714300"}, {"first_name"=>"Sante", "last_name"=>"Roperto", "scopus_author_id"=>"6508365316"}, {"first_name"=>"Raffaele", "last_name"=>"Velotta", "scopus_author_id"=>"7003488328"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-85012050639", "sgr"=>"85012050639", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0171754", "pmid"=>"28182720", "isbn"=>"1111111111", "pui"=>"614362827"}, "id"=>"b345035e-db78-3c5e-b0e3-8933af2748c4", "abstract"=>"BACKGROUND Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of the contaminants in water as well as solid matrices. Although important to be detected, small analytes (few hundreds of Daltons) are an issue in biosensing since the signal they induce in the transducer, and specifically in a Quartz-Crystal Microbalance, is undetectable. A pesticide like parathion (M = 292 Da) is a typical example of contaminant for which a signal amplification procedure is desirable. METHODS/FINDINGS The ballasting of the analyte by gold nanoparticles has been already applied to heavy target as proteins or bacteria to improve the limit of detection. In this paper, we extend the application of such a method to small analytes by showing that once the working surface of a Quartz-Crystal Microbalance (QCM) has been properly functionalized, a limit of detection lower than 1 ppb is reached for parathion. The effective surface functionalization is achieved by immobilizing antibodies upright oriented on the QCM gold surface by a simple photochemical technique (Photonic Immobilization Technique, PIT) based on the UV irradiation of the antibodies, whereas a simple protocol provided by the manufacturer is applied to functionalize the gold nanoparticles. Thus, in a non-competitive approach, the small analyte is made detectable by weighing it down through a \"sandwich protocol\" with a second antibody tethered to heavy gold nanoparticles. The immunosensor has been proved to be effective against the parathion while showing no cross reaction when a mixture of compounds very similar to parathion is analyzed. CONCLUSION/SIGNIFICANCE The immunosensor described in this paper can be easily applied to any small molecule for which polyclonal antibodies are available since both the functionalization procedure of the QCM probe surface and gold nanoparticle can be applied to any IgG, thereby making our device of general application in terms of target analyte.", "link"=>"http://www.mendeley.com/research/effective-antibodies-immobilization-functionalized-nanoparticles-quartzcrystal-microbalancebased-imm", "reader_count"=>22, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Master"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Master"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Materials Science"=>3, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Physics and Astronomy"=>5, "Chemistry"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Materials Science"=>{"Materials Science"=>3}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Physics and Astronomy"=>{"Physics and Astronomy"=>5}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>3}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>1}

Scopus | Further Information

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