A novel approach to eliminate detection of contaminating Staphylococcal species introduced during clinical testing
Publication Date
February 22, 2017
Authors
Wanyuan Ao, Adrianne Clifford, Maylene Corpuz & Robert Jenison
Volume
12
Issue
2
Pages
e0171915
DOI
https://dx.plos.org/10.1371/journal.pone.0171915
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0171915
Scopus
85013428381
Mendeley
http://www.mendeley.com/research/novel-approach-eliminate-detection-contaminating-staphylococcal-species-introduced-during-clinical-t
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Mendeley | Further Information

{"title"=>"A novel approach to eliminate detection of contaminating Staphylococcal species introduced during clinical testing", "type"=>"journal", "authors"=>[{"first_name"=>"Wanyuan", "last_name"=>"Ao", "scopus_author_id"=>"55259792900"}, {"first_name"=>"Adrianne", "last_name"=>"Clifford", "scopus_author_id"=>"57193390894"}, {"first_name"=>"Maylene", "last_name"=>"Corpuz", "scopus_author_id"=>"55323913900"}, {"first_name"=>"Robert", "last_name"=>"Jenison", "scopus_author_id"=>"7003330929"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85013428381", "doi"=>"10.1371/journal.pone.0171915", "pui"=>"614515061", "pmid"=>"28225823", "scopus"=>"2-s2.0-85013428381", "issn"=>"19326203", "isbn"=>"1111111111"}, "id"=>"0560f9b3-1c0d-37af-b9f8-1248ba52b6d8", "abstract"=>"We describe here a strategy that can distinguish between Staphylococcus species truly present in a clinical sample from contaminating Staphylococcus species introduced during the testing process. Contaminating Staphylococcus species are present at low levels in PCR reagents and colonize lab personnel. To eliminate detection of contaminants, we describe an approach that utilizes addition of sufficient quantities of either non-target Staphylococcal cells (Staphylococcus succinus or Staphylococcus muscae) or synthetic oligonucleotide templates to helicase dependent isothermal amplification reactions to consume Staphylococcus-specific tuf and mecA gene primers such that contaminating Staphylococcus amplification is suppressed to below assay limits of detection. The suppressor template DNA is designed with perfect homology to the primers used in the assay but an internal sequence that is unrelated to the Staphylococcal species targeted for detection. Input amount of the suppressor is determined by a mathematical model described herein and is demonstrated to completely suppress contaminating levels of Staphylococcus while not negatively impacting the appropriate clinical assay limit of detection. We have applied this approach to improve the specificity of detection of Staphylococcus species present in positive blood cultures using a chip-based array that produces results visible to the unaided eye.", "link"=>"http://www.mendeley.com/research/novel-approach-eliminate-detection-contaminating-staphylococcal-species-introduced-during-clinical-t", "reader_count"=>5, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Postgraduate"=>1, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Postgraduate"=>1, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Medicine and Dentistry"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}}, "group_count"=>0}

Scopus | Further Information

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