Characterization of bacterial community associated with phytoplankton bloom in a eutrophic lake in South Norway using 16S rRNA gene amplicon sequence analysis
Publication Date
March 10, 2017
Authors
Niranjan Nitin Parulekar, Pandurang Kolekar, Andrew Jenkins, Synne Kleiven, et al
Volume
12
Issue
3
Pages
e0173408
DOI
https://dx.plos.org/10.1371/journal.pone.0173408
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0173408
Scopus
85015181656
Mendeley
http://www.mendeley.com/research/characterization-bacterial-community-associated-phytoplankton-bloom-eutrophic-lake-south-norway-usin-1
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Mendeley | Further Information

{"title"=>"Characterization of bacterial community associated with phytoplankton bloom in a eutrophic lake in South Norway using 16S rRNA gene amplicon sequence analysis", "type"=>"journal", "authors"=>[{"first_name"=>"Niranjan", "last_name"=>"Nitin Parulekar", "scopus_author_id"=>"57193608414"}, {"first_name"=>"Pandurang", "last_name"=>"Kolekar", "scopus_author_id"=>"36994553100"}, {"first_name"=>"Andrew", "last_name"=>"Jenkins", "scopus_author_id"=>"57193604296"}, {"first_name"=>"Synne", "last_name"=>"Kleiven", "scopus_author_id"=>"6603462173"}, {"first_name"=>"Hans", "last_name"=>"Utkilen", "scopus_author_id"=>"6603053212"}, {"first_name"=>"Anette", "last_name"=>"Johansen", "scopus_author_id"=>"57193604619"}, {"first_name"=>"Sangeeta", "last_name"=>"Sawant", "scopus_author_id"=>"7102572551"}, {"first_name"=>"Urmila", "last_name"=>"Kulkarni-Kale", "scopus_author_id"=>"6507581306"}, {"first_name"=>"Mohan", "last_name"=>"Kale", "scopus_author_id"=>"7005639073"}, {"first_name"=>"Mona", "last_name"=>"Sæbø", "scopus_author_id"=>"8682006500"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"28282404", "doi"=>"10.1371/journal.pone.0173408", "sgr"=>"85015181656", "isbn"=>"1111111111", "scopus"=>"2-s2.0-85015181656", "issn"=>"19326203", "pui"=>"614752037"}, "id"=>"c3e9da50-f332-3ff9-ae74-0cff2393919b", "abstract"=>"Interactions between different phytoplankton taxa and heterotrophic bacterial communities within aquatic environments can differentially support growth of various heterotrophic bacterial species. In this study, phytoplankton diversity was studied using traditional microscopic techniques and the bacterial communities associated with phytoplankton bloom were studied using High Throughput Sequencing (HTS) analysis of 16S rRNA gene amplicons from the V1-V3 and V3-V4 hypervariable regions. Samples were collected from Lake Akersvannet, a eutrophic lake in South Norway, during the growth season from June to August 2013. Microscopic examination revealed that the phytoplankton community was mostly represented by Cyanobacteria and the dinoflagellate Ceratium hirundinella. The HTS results revealed that Proteobacteria (Alpha, Beta, and Gamma), Bacteriodetes, Cyanobacteria, Actinobacteria and Verrucomicrobia dominated the bacterial community, with varying relative abundances throughout the sampling season. Species level identification of Cyanobacteria showed a mixed population of Aphanizomenon flos-aquae, Microcystis aeruginosa and Woronichinia naegeliana. A significant proportion of the microbial community was composed of unclassified taxa which might represent locally adapted freshwater bacterial groups. Comparison of cyanobacterial species composition from HTS and microscopy revealed quantitative discrepancies, indicating a need for cross validation of results. To our knowledge, this is the first study that uses HTS methods for studying the bacterial community associated with phytoplankton blooms in a Norwegian lake. The study demonstrates the value of considering results from multiple methods when studying bacterial communities.", "link"=>"http://www.mendeley.com/research/characterization-bacterial-community-associated-phytoplankton-bloom-eutrophic-lake-south-norway-usin-1", "reader_count"=>32, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Librarian"=>1, "Researcher"=>6, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>11, "Other"=>2, "Student > Bachelor"=>4, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Librarian"=>1, "Researcher"=>6, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>11, "Other"=>2, "Student > Bachelor"=>4, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Environmental Science"=>4, "Biochemistry, Genetics and Molecular Biology"=>7, "Agricultural and Biological Sciences"=>16, "Immunology and Microbiology"=>1, "Economics, Econometrics and Finance"=>1}, "reader_count_by_subdiscipline"=>{"Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}, "Unspecified"=>{"Unspecified"=>3}, "Environmental Science"=>{"Environmental Science"=>4}}, "reader_count_by_country"=>{"Netherlands"=>1}, "group_count"=>1}

Scopus | Further Information

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