Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells
Publication Date
April 05, 2017
Authors
Nobuyuki Tanaka, Tadahiro Yamashita, Asako Sato, Viola Vogel, et al
Volume
12
Issue
4
Pages
e0173647
DOI
https://dx.plos.org/10.1371/journal.pone.0173647
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0173647
Scopus
85016964882
Mendeley
http://www.mendeley.com/research/simple-agarose-microconfinement-array-machinelearningbased-classification-analyzing-patterned-differ
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells", "type"=>"journal", "authors"=>[{"first_name"=>"Nobuyuki", "last_name"=>"Tanaka", "scopus_author_id"=>"55725499200"}, {"first_name"=>"Tadahiro", "last_name"=>"Yamashita", "scopus_author_id"=>"37666572500"}, {"first_name"=>"Asako", "last_name"=>"Sato", "scopus_author_id"=>"57189696385"}, {"first_name"=>"Viola", "last_name"=>"Vogel", "scopus_author_id"=>"7102834836"}, {"first_name"=>"Yo", "last_name"=>"Tanaka", "scopus_author_id"=>"14061292500"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"doi"=>"10.1371/journal.pone.0173647", "sgr"=>"85016964882", "isbn"=>"1111111111", "pmid"=>"28380036", "issn"=>"19326203", "scopus"=>"2-s2.0-85016964882", "pui"=>"615148876"}, "id"=>"5aff9804-3fbf-3c0e-9206-de61c33df4c6", "abstract"=>"The geometrical confinement of small cell colonies gives differential cues to cells sitting at the periphery versus the core. To utilize this effect, for example to create spatially graded dif-ferentiation patterns of human mesenchymal stem cells (hMSCs) in vitro or to investigate underpinning mechanisms, the confinement needs to be robust for extended time periods. To create highly repeatable micro-fabricated structures for cellular patterning and high-throughput data mining, we employed here a simple casting method to fabricate more than 800 adhesive patches confined by agarose micro-walls. In addition, a machine learning based image processing software was developed (open code) to detect the differentiation patterns of the population of hMSCs automatically. Utilizing the agarose walls, the circular patterns of hMSCs were successfully maintained throughout 15 days of cell culture. After staining lipid droplets and alkaline phosphatase as the markers of adipogenic and osteo-genic differentiation, respectively, the mega-pixels of RGB color images of hMSCs were processed by the software on a laptop PC within several minutes. The image analysis suc-cessfully showed that hMSCs sitting on the more central versus peripheral sections of the adhesive circles showed adipogenic versus osteogenic differentiation as reported previ-ously, indicating the compatibility of patterned agarose walls to conventional microcontact printing. In addition, we found a considerable fraction of undifferentiated cells which are pref-erentially located at the peripheral part of the adhesive circles, even in differentiation-induc-ing culture media. In this study, we thus successfully demonstrated a simple framework for analyzing the patterned differentiation of hMSCs in confined microenvironments, which has a range of applications in biology, including stem cell biology. (2017) Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells. PLoS ONE 12(4):", "link"=>"http://www.mendeley.com/research/simple-agarose-microconfinement-array-machinelearningbased-classification-analyzing-patterned-differ", "reader_count"=>17, "reader_count_by_academic_status"=>{"Researcher"=>4, "Student > Ph. D. Student"=>6, "Other"=>1, "Student > Master"=>2, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>4, "Student > Ph. D. Student"=>6, "Other"=>1, "Student > Master"=>2, "Student > Bachelor"=>3, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Nursing and Health Professions"=>1, "Materials Science"=>1, "Agricultural and Biological Sciences"=>4, "Computer Science"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>4}, "Materials Science"=>{"Materials Science"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Computer Science"=>{"Computer Science"=>2}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United Kingdom"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85016964882"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85016964882?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85016964882&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85016964882&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85016964882", "dc:identifier"=>"SCOPUS_ID:85016964882", "eid"=>"2-s2.0-85016964882", "dc:title"=>"Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells", "dc:creator"=>"Tanaka N.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"12", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2017-04-01", "prism:coverDisplayDate"=>"April 2017", "prism:doi"=>"10.1371/journal.pone.0173647", "citedby-count"=>"10", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Riken", "affiliation-city"=>"Wako", "affiliation-country"=>"Japan"}], "pubmed-id"=>"28380036", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0173647", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true, "freetoread"=>{"value"=>[{"$"=>"all"}, {"$"=>"publisherfullgold"}, {"$"=>"repository"}, {"$"=>"repositoryvor"}, {"$"=>"repositoryam"}]}, "freetoreadLabel"=>{"value"=>[{"$"=>"All Open Access"}, {"$"=>"Gold"}, {"$"=>"Green"}]}}

Counter

  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"121", "xml_views"=>"4", "html_views"=>"724"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"122"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"70"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"2", "html_views"=>"39"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"75"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"147"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"177"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"28", "xml_views"=>"2", "html_views"=>"34"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"3", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"3", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"25", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2021", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"23"}

PMC Usage Stats

  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"16", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"44", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"29", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"38", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"1", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"12"}
Loading … Spinner
There are currently no alerts