In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection
Publication Date
April 26, 2017
Authors
Asami Nishimori, Satoru Konnai, Tomohiro Okagawa, Naoya Maekawa, et al
Volume
12
Issue
4
Pages
e0174916
DOI
https://dx.plos.org/10.1371/journal.pone.0174916
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0174916
Scopus
85018351182
Mendeley
http://www.mendeley.com/research/vitro-vivo-antivirus-activity-antiprogrammed-deathligand-1-pdl1-ratbovine-chimeric-antibody-against
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Mendeley | Further Information

{"title"=>"In vitro and in vivo antivirus activity of an antiprogrammed death-ligand 1 (PD-L1) ratbovine chimeric antibody against bovine leukemia virus infection", "type"=>"journal", "authors"=>[{"first_name"=>"Asami", "last_name"=>"Nishimori", "scopus_author_id"=>"56256373500"}, {"first_name"=>"Satoru", "last_name"=>"Konnai", "scopus_author_id"=>"6602562824"}, {"first_name"=>"Tomohiro", "last_name"=>"Okagawa", "scopus_author_id"=>"54399248900"}, {"first_name"=>"Naoya", "last_name"=>"Maekawa", "scopus_author_id"=>"56209130200"}, {"first_name"=>"Ryoyo", "last_name"=>"Ikebuchi", "scopus_author_id"=>"35746077900"}, {"first_name"=>"Shinya", "last_name"=>"Goto", "scopus_author_id"=>"57194013929"}, {"first_name"=>"Yamato", "last_name"=>"Sajiki", "scopus_author_id"=>"57194020993"}, {"first_name"=>"Yasuhiko", "last_name"=>"Suzuki", "scopus_author_id"=>"8964195600"}, {"first_name"=>"Junko", "last_name"=>"Kohara", "scopus_author_id"=>"8667530400"}, {"first_name"=>"Satoshi", "last_name"=>"Ogasawara", "scopus_author_id"=>"12752725300"}, {"first_name"=>"Yukinari", "last_name"=>"Kato", "scopus_author_id"=>"7404905250"}, {"first_name"=>"Shiro", "last_name"=>"Murata", "scopus_author_id"=>"18134234800"}, {"first_name"=>"Kazuhiko", "last_name"=>"Ohashi", "scopus_author_id"=>"7401968649"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"615713841", "issn"=>"19326203", "isbn"=>"1111111111", "doi"=>"10.1371/journal.pone.0174916", "scopus"=>"2-s2.0-85018351182", "pmid"=>"28445479", "sgr"=>"85018351182"}, "id"=>"51e87474-2e16-3575-afe4-575561458347", "abstract"=>"Programmed death-1 (PD-1), an immunoinhibitory receptor on T cells, is known to be involved in immune evasion through its binding to PD-ligand 1 (PD-L1) in many chronic diseases. We previously found that PD-L1 expression was upregulated in cattle infected with bovine leukemia virus (BLV) and that an antibody that blocked the PD-1/PD-L1 interaction reactivated T-cell function in vitro. Therefore, this study assessed its antivirus activities in vivo. First, we inoculated the anti-bovine PD-L1 rat monoclonal antibody 4G12 into a BLV-infected cow. However, this did not induce T-cell proliferation or reduction of BLV provirus loads during the test period, and only bound to circulating IgM+ B cells until one week post-inoculation. We hypothesized that this lack of in vivo effects was due to its lower stability in cattle and so established an anti-PD-L1 rat-bovine chimeric antibody (Boch4G12). Boch4G12 was able to bind specifically with bovine PD-L1, interrupt the PD-1/PD-L1 interaction, and activate the immune response in both healthy and BLV-infected cattle in vitro. Therefore, we experimentally infected a healthy calf with BLV and inoculated it intravenously with 1 mg/kg of Boch4G12 once it reached the aleukemic (AL) stage. Cultivation of peripheral blood mononuclear cells (PBMCs) isolated from the tested calf indicated that the proliferation of CD4+ T cells was increased by Boch4G12 inoculation, while BLV provirus loads were significantly reduced, clearly demonstrating that this treatment induced antivirus activities. Therefore, further studies using a large number of animals are required to support its efficacy for clinical application.", "link"=>"http://www.mendeley.com/research/vitro-vivo-antivirus-activity-antiprogrammed-deathligand-1-pdl1-ratbovine-chimeric-antibody-against", "reader_count"=>6, "reader_count_by_academic_status"=>{"Student > Ph. D. Student"=>2, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Student > Ph. D. Student"=>2, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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