Comparative analysis of the sensitivity of metagenomic sequencing and PCR to detect a biowarfare simulant (Bacillus atrophaeus) in soil samples
Publication Date
May 04, 2017
Authors
Delphine Plaire, Simon Puaud, Marie Claude Marsolier Kergoat & Jean Marc Elalouf
Volume
12
Issue
5
Pages
e0177112
DOI
https://dx.plos.org/10.1371/journal.pone.0177112
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0177112
Scopus
85019086217
Mendeley
http://www.mendeley.com/research/comparative-analysis-sensitivity-metagenomic-sequencing-pcr-detect-biowarfare-simulant-bacillus-atro
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Mendeley | Further Information

{"title"=>"Comparative analysis of the sensitivity of metagenomic sequencing and PCR to detect a biowarfare simulant (Bacillus atrophaeus) in soil samples", "type"=>"journal", "authors"=>[{"first_name"=>"Delphine", "last_name"=>"Plaire", "scopus_author_id"=>"57194192952"}, {"first_name"=>"Simon", "last_name"=>"Puaud", "scopus_author_id"=>"57194183674"}, {"first_name"=>"Marie Claude", "last_name"=>"Marsolier-Kergoat", "scopus_author_id"=>"6506166660"}, {"first_name"=>"Jean Marc", "last_name"=>"Elalouf", "scopus_author_id"=>"7003605321"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85019086217", "pmid"=>"28472119", "pui"=>"615955922", "issn"=>"19326203", "isbn"=>"1111111111", "scopus"=>"2-s2.0-85019086217", "doi"=>"10.1371/journal.pone.0177112"}, "id"=>"b040129f-31b5-31e4-b49a-208aca637467", "abstract"=>"To evaluate the sensitivity of high-throughput DNA sequencing for monitoring biowarfare agents in the environment, we analysed soil samples inoculated with different amounts of Bacillus atrophaeus, a surrogate organism for Bacillus anthracis. The soil samples considered were a poorly carbonated soil of the silty sand class, and a highly carbonated soil of the silt class. Control soil samples and soil samples inoculated with 10, 103, or 105 cfu were processed for DNA extraction. About 1% of the DNA extracts was analysed through the sequencing of more than 108 reads. Similar amounts of extracts were also studied for Bacillus atrophaeus DNA content by real-time PCR. We demonstrate that, for both soils, high-throughput sequencing is at least equally sensitive than real-time PCR to detect Bacillus atrophaeus DNA. We conclude that metagenomics allows the detection of less than 10 ppm of DNA from a biowarfare simulant in complex environmental samples.", "link"=>"http://www.mendeley.com/research/comparative-analysis-sensitivity-metagenomic-sequencing-pcr-detect-biowarfare-simulant-bacillus-atro", "reader_count"=>8, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>3, "Computer Science"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Computer Science"=>{"Computer Science"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "group_count"=>0}

Scopus | Further Information

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