3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity
Publication Date
June 06, 2017
Authors
Michelle T. Poldervaart, Birgit Goversen, Mylene De Ruijter, Anna Abbadessa, et al
Volume
12
Issue
6
Pages
e0177628
DOI
https://dx.plos.org/10.1371/journal.pone.0177628
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0177628
Scopus
85020167494
Mendeley
http://www.mendeley.com/research/3d-bioprinting-methacrylated-hyaluronic-acid-meha-hydrogel-intrinsic-osteogenicity
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity", "type"=>"journal", "authors"=>[{"first_name"=>"Michelle T.", "last_name"=>"Poldervaart", "scopus_author_id"=>"33167754800"}, {"first_name"=>"Birgit", "last_name"=>"Goversen", "scopus_author_id"=>"57192949633"}, {"first_name"=>"Mylene", "last_name"=>"De Ruijter", "scopus_author_id"=>"57194457845"}, {"first_name"=>"Anna", "last_name"=>"Abbadessa", "scopus_author_id"=>"55201274600"}, {"first_name"=>"Ferry P.W.", "last_name"=>"Melchels", "scopus_author_id"=>"15729979600"}, {"first_name"=>"F. Cumhur", "last_name"=>"Öner", "scopus_author_id"=>"7006040338"}, {"first_name"=>"Wouter J.A.", "last_name"=>"Dhert", "scopus_author_id"=>"7004077496"}, {"first_name"=>"Tina", "last_name"=>"Vermonden", "scopus_author_id"=>"6602798409"}, {"first_name"=>"Jacqueline", "last_name"=>"Alblas", "scopus_author_id"=>"6701801021"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-85020167494", "sgr"=>"85020167494", "doi"=>"10.1371/journal.pone.0177628", "pui"=>"616620376", "pmid"=>"28586346", "isbn"=>"1111111111", "issn"=>"19326203"}, "id"=>"68042b8f-17e8-30da-a0af-9572e7799638", "abstract"=>"In bone regenerative medicine there is a need for suitable bone substitutes. Hydrogels have excellent biocompatible and biodegradable characteristics, but their visco-elastic properties limit their applicability, especially with respect to 3D bioprinting. In this study, we modified the naturally occurring extracellular matrix glycosaminoglycan hyaluronic acid (HA), in order to yield photo-crosslinkable hydrogels with increased mechanical stiffness and long-term stability, and with minimal decrease in cytocompatibility. Application of these tailor-made methacrylated hyaluronic acid (MeHA) gels for bone tissue engineering and 3D bioprinting was the subject of investigation. Visco-elastic properties of MeHA gels, measured by rheology and dynamic mechanical analysis, showed that irradiation of the hydrogels with UV light led to increased storage moduli and elastic moduli, indicating increasing gel rigidity. Subsequently, human bone marrow derived mesenchymal stromal cells (MSCs) were incorporated into MeHA hydrogels, and cell viability remained 64.4% after 21 days of culture. Osteogenic differentiation of MSCs occurred spontaneously in hydrogels with high concentrations of MeHA polymer, in absence of additional osteogenic stimuli. Addition of bone morphogenetic protein-2 (BMP-2) to the culture medium further increased osteogenic differentiation, as evidenced by increased matrix mineralisation. MeHA hydrogels demonstrated to be suitable for 3D bioprinting, and were printed into porous and anatomically shaped scaffolds. Taken together, photosensitive MeHA-based hydrogels fulfilled our criteria for cellular bioprinted bone constructs within a narrow window of concentration.", "link"=>"http://www.mendeley.com/research/3d-bioprinting-methacrylated-hyaluronic-acid-meha-hydrogel-intrinsic-osteogenicity", "reader_count"=>54, "reader_count_by_academic_status"=>{"Unspecified"=>4, "Professor > Associate Professor"=>1, "Researcher"=>10, "Student > Ph. D. Student"=>15, "Student > Postgraduate"=>2, "Student > Master"=>11, "Student > Bachelor"=>9, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>4, "Professor > Associate Professor"=>1, "Researcher"=>10, "Student > Ph. D. Student"=>15, "Student > Postgraduate"=>2, "Student > Master"=>11, "Student > Bachelor"=>9, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>14, "Unspecified"=>9, "Biochemistry, Genetics and Molecular Biology"=>10, "Materials Science"=>5, "Medicine and Dentistry"=>2, "Agricultural and Biological Sciences"=>4, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>2, "Chemical Engineering"=>2, "Chemistry"=>5}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>14}, "Materials Science"=>{"Materials Science"=>5}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>5}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Unspecified"=>{"Unspecified"=>9}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}, "Chemical Engineering"=>{"Chemical Engineering"=>2}}, "reader_count_by_country"=>{"Spain"=>1}, "group_count"=>5}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85020167494"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85020167494?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85020167494&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85020167494&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85020167494", "dc:identifier"=>"SCOPUS_ID:85020167494", "eid"=>"2-s2.0-85020167494", "dc:title"=>"3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity", "dc:creator"=>"Poldervaart M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"12", "prism:issueIdentifier"=>"6", "prism:pageRange"=>nil, "prism:coverDate"=>"2017-06-01", "prism:coverDisplayDate"=>"June 2017", "prism:doi"=>"10.1371/journal.pone.0177628", "citedby-count"=>"24", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University Medical Center Utrecht", "affiliation-city"=>"Utrecht", "affiliation-country"=>"Netherlands"}], "pubmed-id"=>"28586346", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0177628", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Counter

  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"189", "xml_views"=>"7", "html_views"=>"332"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"85", "xml_views"=>"4", "html_views"=>"132"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"92", "xml_views"=>"1", "html_views"=>"154"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"116", "xml_views"=>"4", "html_views"=>"200"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"100", "xml_views"=>"1", "html_views"=>"217"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"79", "xml_views"=>"0", "html_views"=>"289"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"41", "xml_views"=>"0", "html_views"=>"361"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"101", "xml_views"=>"1", "html_views"=>"179"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"70", "xml_views"=>"0", "html_views"=>"111"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"105", "xml_views"=>"0", "html_views"=>"131"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"101", "xml_views"=>"1", "html_views"=>"113"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"52", "xml_views"=>"1", "html_views"=>"96"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"128", "xml_views"=>"5", "html_views"=>"113"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"70", "xml_views"=>"3", "html_views"=>"150"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"136", "xml_views"=>"2", "html_views"=>"149"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"58", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"76", "xml_views"=>"1", "html_views"=>"103"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"76", "xml_views"=>"1", "html_views"=>"141"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"46", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"61", "xml_views"=>"2", "html_views"=>"108"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"55", "xml_views"=>"0", "html_views"=>"160"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"102", "xml_views"=>"0", "html_views"=>"148"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"86", "xml_views"=>"0", "html_views"=>"153"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"77", "xml_views"=>"0", "html_views"=>"137"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"93", "xml_views"=>"1", "html_views"=>"143"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"84", "xml_views"=>"2", "html_views"=>"94"}

PMC Usage Stats

  • {"unique-ip"=>"28", "full-text"=>"30", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"48", "full-text"=>"32", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"26", "full-text"=>"30", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"50", "full-text"=>"51", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"38", "full-text"=>"41", "pdf"=>"26", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"38", "full-text"=>"31", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"37", "full-text"=>"34", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"45", "full-text"=>"50", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"25", "full-text"=>"31", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"63", "full-text"=>"68", "pdf"=>"25", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"46", "full-text"=>"44", "pdf"=>"23", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"49", "full-text"=>"49", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"69", "full-text"=>"80", "pdf"=>"22", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"40", "full-text"=>"49", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"51", "full-text"=>"49", "pdf"=>"18", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"43", "full-text"=>"43", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"53", "full-text"=>"52", "pdf"=>"24", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"53", "full-text"=>"59", "pdf"=>"20", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"53", "full-text"=>"64", "pdf"=>"16", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"66", "full-text"=>"66", "pdf"=>"15", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"48", "full-text"=>"46", "pdf"=>"18", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"56", "full-text"=>"60", "pdf"=>"22", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"82", "full-text"=>"99", "pdf"=>"33", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2019", "month"=>"5"}
Loading … Spinner
There are currently no alerts