An androgen response element driven reporter assay for the detection of androgen receptor activity in prostate cells
Publication Date
June 01, 2017
Authors
Waqas Azeem, Margrete Reime Hellem, Jan Roger Olsen, Yaping Hua, et al
Volume
12
Issue
6
Pages
e0177861
DOI
https://dx.plos.org/10.1371/journal.pone.0177861
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0177861
Scopus
85020015380
Mendeley
http://www.mendeley.com/research/androgen-response-element-driven-reporter-assay-detection-androgen-receptor-activity-prostate-cells
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Mendeley | Further Information

{"title"=>"An androgen response element driven reporter assay for the detection of androgen receptor activity in prostate cells", "type"=>"journal", "authors"=>[{"first_name"=>"Waqas", "last_name"=>"Azeem", "scopus_author_id"=>"55973306600"}, {"first_name"=>"Margrete Reime", "last_name"=>"Hellem", "scopus_author_id"=>"55604940400"}, {"first_name"=>"Jan Roger", "last_name"=>"Olsen", "scopus_author_id"=>"36680506800"}, {"first_name"=>"Yaping", "last_name"=>"Hua", "scopus_author_id"=>"21534351100"}, {"first_name"=>"Kristo", "last_name"=>"Marvyin", "scopus_author_id"=>"57190047723"}, {"first_name"=>"Yi", "last_name"=>"Qu", "scopus_author_id"=>"25936619500"}, {"first_name"=>"Biaoyang", "last_name"=>"Lin", "scopus_author_id"=>"35199806000"}, {"first_name"=>"Xisong", "last_name"=>"Ke", "scopus_author_id"=>"7006106345"}, {"first_name"=>"Anne Margrete", "last_name"=>"Yan", "scopus_author_id"=>"55211689100"}, {"first_name"=>"Karl Henning", "last_name"=>"Kalland", "scopus_author_id"=>"7003542134"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85020015380", "pmid"=>"28570625", "pui"=>"616529612", "issn"=>"19326203", "isbn"=>"1111111111", "scopus"=>"2-s2.0-85020015380", "doi"=>"10.1371/journal.pone.0177861"}, "id"=>"4a0686eb-389e-3d83-a2d0-c569f2032bb4", "abstract"=>"The androgen receptor (AR) transcription factor plays a key role in the development and progression of prostate cancer, as is evident from the efficacy of androgen-deprivation therapy, AR is also the most frequently mutated gene, in castration resistant prostate cancer (CRPC). AR has therefore become an even more attractive therapeutic target in aggressive and disseminated prostate cancer. To investigate mechanisms of AR and AR target gene activation in different subpopulations of prostate cancer cells, a toolkit of AR expressor and androgen response element (ARE) reporter vectors were developed. Three ARE reporter vectors were constructed with different ARE consensus sequences in promoters linked to either fluorescence or luciferase reporter genes in lentiviral vector backbones. Cell lines transduced with the different vectors expressed the reporters in an androgen-dependent way according to fluorescence microscopy, flow cytometry and multi-well fluorescent and luminescence assays. Interestingly, the background reporter activity in androgen-depleted medium was significantly higher in LNCaP cells compared to the prostate transit amplifying epithelial cell lines, EP156T-AR and 957E/hTERT-AR with exogenous AR. The androgen-induced signal to background was much higher in the latter benign prostate cells than in LNCaP cells. Androgen-independent nuclear localization of AR was seen in LNCaP cells and reduced ARE-signaling was seen following treatment with abiraterone, an androgen synthesis inhibitor. The ARE reporter activity was significantly stronger when stimulated by androgens than by β-estradiol, progesterone and dexamethasone in all tested cell types. Finally, no androgen-induced ARE reporter activity was observed in tumorigenic mesenchymal progeny cells of EP156T cells following epithelial to mesenchymal transition. This underscores the observation that expression of the classical luminal differentiation transcriptome is restricted in mesenchymal type cells with or without AR expression, and presence of androgen.", "link"=>"http://www.mendeley.com/research/androgen-response-element-driven-reporter-assay-detection-androgen-receptor-activity-prostate-cells", "reader_count"=>15, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>3, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Other"=>1, "Student > Bachelor"=>3, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>3, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Other"=>1, "Student > Bachelor"=>3, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>8, "Medicine and Dentistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>8}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>1}

Scopus | Further Information

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