Low doses of cholera toxin and its mediator cAMP induce CTLA-2 secretion by dendritic cells to enhance regulatory T cell conversion
Publication Date
July 31, 2017
Authors
Cinthia Silva Vilches, Katrien Pletinckx, Miriam Lohnert, Vladimir Pavlovic, et al
Volume
12
Issue
7
Pages
e0178114
DOI
https://dx.plos.org/10.1371/journal.pone.0178114
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0178114
Scopus
85026360187
Mendeley
http://www.mendeley.com/research/low-doses-cholera-toxin-mediator-camp-induce-ctla2-secretion-dendritic-cells-enhance-regulatory-t-ce
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Mendeley | Further Information

{"title"=>"Low doses of cholera toxin and its mediator cAMP induce CTLA-2 secretion by dendritic cells to enhance regulatory T cell conversion", "type"=>"journal", "authors"=>[{"first_name"=>"Cinthia", "last_name"=>"Silva-Vilches", "scopus_author_id"=>"57195052691"}, {"first_name"=>"Katrien", "last_name"=>"Pletinckx", "scopus_author_id"=>"24339060500"}, {"first_name"=>"Miriam", "last_name"=>"Lohnert", "scopus_author_id"=>"57195226407"}, {"first_name"=>"Vladimir", "last_name"=>"Pavlovic", "scopus_author_id"=>"56343479300"}, {"first_name"=>"Diyaaeldin", "last_name"=>"Ashour", "scopus_author_id"=>"57195225904"}, {"first_name"=>"Vini", "last_name"=>"John", "scopus_author_id"=>"57195226227"}, {"first_name"=>"Emilia", "last_name"=>"Vendelova", "scopus_author_id"=>"56896125000"}, {"first_name"=>"Susanne", "last_name"=>"Kneitz", "scopus_author_id"=>"6603394069"}, {"first_name"=>"Jie", "last_name"=>"Zhou", "scopus_author_id"=>"57195235627"}, {"first_name"=>"Rena", "last_name"=>"Chen", "scopus_author_id"=>"57195225430"}, {"first_name"=>"Thomas", "last_name"=>"Reinheckel", "scopus_author_id"=>"7003754266"}, {"first_name"=>"Thomas D.", "last_name"=>"Mueller", "scopus_author_id"=>"7201804828"}, {"first_name"=>"Jochen", "last_name"=>"Bodem", "scopus_author_id"=>"6601953727"}, {"first_name"=>"Manfred B.", "last_name"=>"Lutz", "scopus_author_id"=>"7201814005"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pui"=>"617564256", "doi"=>"10.1371/journal.pone.0178114", "sgr"=>"85026360187", "isbn"=>"1111111111", "pmid"=>"28759565", "scopus"=>"2-s2.0-85026360187"}, "id"=>"6dd9c9fd-f900-37ae-b3a4-45c7ba4d9eb5", "abstract"=>"© 2017 Silva-Vilches et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Immature or semi-mature dendritic cells (DCs) represent tolerogenic maturation stages that can convert naive T cells into Foxp3 + induced regulatory T cells (iTreg). Here we found that murine bone marrow-derived DCs (BM-DCs) treated with cholera toxin (CT) matured by up-regulating MHC-II and costimulatory molecules using either high or low doses of CT (CT hi , CT lo ) or with cAMP, a known mediator CT signals. However, all three conditions also induced mRNA of both isoforms of the tolerogenic molecule cytotoxic T lymphocyte antigen 2 (CTLA-2α and CTLA-2β). Only DCs matured under CT hi conditions secreted IL-1β, IL-6 and IL-23 leading to the instruction of Th17 cell polarization. In contrast, CT lo - or cAMP-DCs resembled semi-mature DCs and enhanced TGF-β-dependent Foxp3 + iTreg conversion. iTreg conversion could be reduced using siRNA blocking of CTLA-2 and reversely, addition of recombinant CTLA-2α increased iTreg conversion in vitro. Injection of CT lo - or cAMP-DCs exerted MOG peptide-specific protective effects in experimental autoimmune encephalomyelitis (EAE) by inducing Foxp3 + Tregs and reducing Th17 responses. Together, we identified CTLA-2 production by DCs as a novel tolerogenic mediator of TGF-β-mediated iTreg induction in vitro and in vivo. The CT-induced and cAMP-mediated up-regulation of CTLA-2 also may point to a novel immune evasion mechanism of Vibrio cholerae.", "link"=>"http://www.mendeley.com/research/low-doses-cholera-toxin-mediator-camp-induce-ctla2-secretion-dendritic-cells-enhance-regulatory-t-ce", "reader_count"=>4, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Master"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Master"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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