Reprimo tissue-specific expression pattern is conserved between zebrafish and human
Publication Date
May 31, 2017
Authors
Ricardo J. Figueroa, Gonzalo Carrasco Avino, Ignacio A. Wichmann, Martin Lange, et al
Volume
12
Issue
5
Pages
e0178274
DOI
https://dx.plos.org/10.1371/journal.pone.0178274
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0178274
Scopus
85019990202
Mendeley
http://www.mendeley.com/research/reprimo-tissuespecific-expression-pattern-conserved-between-zebrafish-human
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Mendeley | Further Information

{"title"=>"Reprimo tissue-specific expression pattern is conserved between zebrafish and human", "type"=>"journal", "authors"=>[{"first_name"=>"Ricardo J.", "last_name"=>"Figueroa", "scopus_author_id"=>"57194409123"}, {"first_name"=>"Gonzalo", "last_name"=>"Carrasco-Avino", "scopus_author_id"=>"56199003400"}, {"first_name"=>"Ignacio A.", "last_name"=>"Wichmann", "scopus_author_id"=>"56825672400"}, {"first_name"=>"Martin", "last_name"=>"Lange", "scopus_author_id"=>"57194418006"}, {"first_name"=>"Gareth I.", "last_name"=>"Owen", "scopus_author_id"=>"35571266900"}, {"first_name"=>"Arndt F.", "last_name"=>"Siekmann", "scopus_author_id"=>"15923667100"}, {"first_name"=>"Alejandro H.", "last_name"=>"Corvalán", "scopus_author_id"=>"6602269865"}, {"first_name"=>"Juan C.", "last_name"=>"Opazo", "scopus_author_id"=>"6602749818"}, {"first_name"=>"Julio D.", "last_name"=>"Amigo", "scopus_author_id"=>"15046523600"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"616527519", "sgr"=>"85019990202", "issn"=>"19326203", "pmid"=>"28562620", "scopus"=>"2-s2.0-85019990202", "doi"=>"10.1371/journal.pone.0178274", "isbn"=>"1111111111"}, "id"=>"0fd9661f-6f09-3557-a4b9-61e02b579498", "abstract"=>"Reprimo (RPRM), a member of the RPRM gene family, is a tumor-suppressor gene involved in the regulation of the p53-mediated cell cycle arrest at G2/M. RPRM has been associated with malignant tumor progression and proposed as a potential biomarker for early cancer detection. However, the expression and role of RPRM, as well as its family, are poorly understood and their physiology is as yet unstudied. In this scenario, a model system like the zebrafish could serve to dissect the role of the RPRM family members in vivo. Phylogenetic analysis reveals that RPRM and RPRML have been differentially retained by most species throughout vertebrate evolution, yet RPRM3 has been retained only in a small group of distantly related species, including zebrafish. Herein, we characterized the spatiotemporal expression of RPRM (present in zebrafish as an infraclass duplication rprma/rprmb), RPRML and RPRM3 in the zebrafish. By whole-mount in situ hybridization (WISH) and fluorescent in situ hybridization (FISH), we demonstrate that rprm (rprma/rprmb) and rprml show a similar spatiotemporal expression profile during zebrafish development. At early developmental stages rprmb is expressed in somites. After one day post-fertilization, rprm (rprma/rprmb) and rprml are expressed in the notochord, brain, blood vessels and digestive tube. On the other hand, rprm3 shows the most unique expression profile, being expressed only in the central nervous system (CNS). We assessed the expression patterns of RPRM gene transcripts in adult zebrafish and human RPRM protein product in tissue samples by RT-qPCR and immunohistochemistry (IHC) staining, respectively. Strikingly, tissue-specific expression patterns of the RPRM transcripts and protein are conserved between zebrafish and humans. We propose the zebrafish as a powerful tool to elucidate the both physiological and pathological roles of the RPRM gene family.", "link"=>"http://www.mendeley.com/research/reprimo-tissuespecific-expression-pattern-conserved-between-zebrafish-human", "reader_count"=>9, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>2, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>2, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>5, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}}, "group_count"=>0}

Scopus | Further Information

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