Molecular diversity and frequency of the diarrheagenic enteric protozoan Giardia duodenalis and Cryptosporidium spp. in a hospital setting in Northern Spain
Publication Date
June 15, 2017
Journal
PLOS ONE
Authors
José Manuel Azcona-Gutiérrez, Aida de Lucio, Marta Hernández-de-Mingo, Concepción García-García, et al
Volume
12
Issue
6
Pages
e0178575
DOI
https://dx.plos.org/10.1371/journal.pone.0178575
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0178575
Scopus
85021150045
Mendeley
http://www.mendeley.com/research/molecular-diversity-frequency-diarrheagenic-enteric-protozoan-giardia-duodenalis-cryptosporidium-spp
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Mendeley | Further Information

{"title"=>"Molecular diversity and frequency of the diarrheagenic enteric protozoan Giardia duodenalis and Cryptosporidium spp. in a hospital setting in Northern Spain", "type"=>"journal", "authors"=>[{"first_name"=>"José Manuel", "last_name"=>"Azcona-Gutiérrez", "scopus_author_id"=>"24400710500"}, {"first_name"=>"Aida", "last_name"=>"de Lucio", "scopus_author_id"=>"6504552286"}, {"first_name"=>"Marta", "last_name"=>"Hernández-De-Mingo", "scopus_author_id"=>"57189574390"}, {"first_name"=>"Concepción", "last_name"=>"García-García", "scopus_author_id"=>"55884208100"}, {"first_name"=>"Luis Miguel", "last_name"=>"Soria-Blanco", "scopus_author_id"=>"57194606084"}, {"first_name"=>"Lucía", "last_name"=>"Morales", "scopus_author_id"=>"57193110932"}, {"first_name"=>"María", "last_name"=>"Aguilera", "scopus_author_id"=>"56441105000"}, {"first_name"=>"Isabel", "last_name"=>"Fuentes", "scopus_author_id"=>"7003436576"}, {"first_name"=>"David", "last_name"=>"Carmena", "scopus_author_id"=>"8521321600"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-85021150045", "sgr"=>"85021150045", "doi"=>"10.1371/journal.pone.0178575", "pui"=>"616860930", "pmid"=>"28617836", "isbn"=>"1111111111", "issn"=>"19326203"}, "id"=>"9708b73b-5d9a-3be7-8500-99e62fd3df10", "abstract"=>"BACKGROUND: Human giardiosis and cryptosporidiosis are caused by the enteric protozoan parasites Giardia duodenalis and Cryptosporidium spp. Both pathogens are major contributors to the global burden of diarrhoeal disease, affecting primarily children and immunodebilitated individuals in resource-poor settings. Giardiosis and cryptosporidiosis also represent an important, often underestimate, public health threat in developed countries. In Spain only limited information is currently available on the epidemiology of these infections. Molecular data on the diversity, frequency, geographical distribution, and seasonality of G. duodenalis assemblages/sub-assemblages and Cryptosporidium species/sub-genotypes are particularly scarce. METHODS: A longitudinal molecular epidemiological survey was conducted between July 2015 to September 2016 in patients referred to or attended at the Hospital San Pedro (La Rioja, Northern Spain) that tested positive for G. duodenalis (N = 106) or Cryptosporidium spp. (N = 103) by direct microscopy and/or a rapid lateral flow immunochromatographic assay. G. duodenalis infections were subsequently confirmed by real-time PCR and positive isolates assessed by multi-locus sequence genotyping of the glutamate dehydrogenase and beta-giardin genes of the parasite. Cryptosporidium species and sub-genotypes were investigated at the 60 kDa glycoprotein or the small subunit ribosomal RNA genes of the parasite. Sociodemographic and clinical parameters of infected patients were also gathered and analysed. PRINCIPAL FINDINGS: Out of 90 G. duodenalis-positive isolates by real-time PCR a total of 16 isolates were successfully typed. AII (44%, 7/16) was the most prevalent sub-assemblage found, followed by BIV (31%, 5/16) and BIII (19%, 3/16). A discordant genotype result AII/AIII was identified in an additional (6%, 1/16) isolate. No mixed infections A+B were detected. Similarly, a total of 81 Cryptosporidium spp. isolates were successfully typed, revealing the presence of C. hominis (81%, 66/81) and C. parvum (19%, 15/81). Obtained GP60 sequences were assigned to sub-type families Ib (73%, 59/81) within C. hominis, and IIa (7%, 6/81) and IId (2%, 2/81) within C. parvum. A marked inter-annual variation in Cryptosporidium cases was observed. CONCLUSIONS: Human giardiasis and cryptosporidiosis are commonly identified in patients seeking medical care in Northern Spain and represent a more important health concern than initially thought. Assemblage A within G. duodenalis and sub-genotype IbA10G2 within C. hominis were the genetic variants of these parasite species more frequently found circulating in the population under study. Molecular data presented here seem to suggest that G. duodenalis and Cryptosporidium infections arise through anthroponotic rather than zoonotic transmission in this Spanish region.", "link"=>"http://www.mendeley.com/research/molecular-diversity-frequency-diarrheagenic-enteric-protozoan-giardia-duodenalis-cryptosporidium-spp", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>4, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>4, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Medicine and Dentistry"=>2, "Agricultural and Biological Sciences"=>2, "Veterinary Science and Veterinary Medicine"=>3, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>4}, "Environmental Science"=>{"Environmental Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>3}}, "group_count"=>1}

Scopus | Further Information

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