Severity classification of repeated isoflurane anesthesia in C57BL/6JRj mice—Assessing the degree of distress
Publication Date
June 15, 2017
Authors
Katharina Hohlbaum, Bettina Bert, Silke Dietze, Rupert Palme, et al
Volume
12
Issue
6
Pages
e0179588
DOI
https://dx.plos.org/10.1371/journal.pone.0179588
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0179588
Scopus
85021109778
Mendeley
http://www.mendeley.com/research/severity-classification-repeated-isoflurane-anesthesia-c57bl6jrj-mice-assessing-degree-distress
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Mendeley | Further Information

{"title"=>"Severity classification of repeated isoflurane anesthesia in C57BL/6JRj mice - Assessing the degree of distress", "type"=>"journal", "authors"=>[{"first_name"=>"Katharina", "last_name"=>"Hohlbaum", "scopus_author_id"=>"57194596190"}, {"first_name"=>"Bettina", "last_name"=>"Bert", "scopus_author_id"=>"6602691602"}, {"first_name"=>"Silke", "last_name"=>"Dietze", "scopus_author_id"=>"6602472811"}, {"first_name"=>"Rupert", "last_name"=>"Palme", "scopus_author_id"=>"55119967900"}, {"first_name"=>"Heidrun", "last_name"=>"Fink", "scopus_author_id"=>"57072781700"}, {"first_name"=>"Christa", "last_name"=>"Thöne-Reineke", "scopus_author_id"=>"6603058914"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85021109778", "doi"=>"10.1371/journal.pone.0179588", "pui"=>"616860893", "pmid"=>"28617851", "scopus"=>"2-s2.0-85021109778", "issn"=>"19326203", "isbn"=>"1111111111"}, "id"=>"9d4c2944-db04-3a15-affa-a095ca0fa0c6", "abstract"=>"According to the EU Directive 2010/63, the severity of a procedure has to be classified as mild, moderate or severe. General anesthesia is thought to be mild, but the Directive does not differentiate between single and repeated anesthesia. Therefore, we investigated the impact of repeated administration of isoflurane, the most commonly used inhalation anesthetic, on the well-being of adult C57BL/6JRj mice, in comparison to single administrations and to untreated animals, when applied six times for 45 min at an interval of 3-4 days. For the animals anesthetized, excitations, phases of anesthesia, and vital parameters were monitored. Well-being after anesthesia was assessed using a behavioral test battery including luxury behavior like burrowing and nest building behavior, the Mouse Grimace Scale (MGS), the free exploratory paradigm for anxiety-related behavior, home cage activity and the rotarod test for activity, as well as food intake and body weight. Additionally, hair corticosterone and fecal corticosterone metabolites were measured. Our results show that nest building behavior, home cage activity, body weight, and corticosterone concentrations were not influenced by anesthesia, whereas changes in burrowing behavior, the MGS, food intake, and the free exploratory behavior indicated that the well-being of the mice was more affected by repeated than single isoflurane anesthesia. This effect depended on the sex of the animals, with female mice being more susceptible than male mice. However, repeated isoflurane anesthesia caused only short-term mild distress and impairment of well-being, mainly in the immediate postanesthetic period. Well-being stabilized at 8 days after the last anesthesia, at the latest. Therefore, we conclude that when using our anesthesia protocol, the severity of both single and repeated isoflurane anesthesia in C57BL/6JRj mice can be classified as mild. However, within the mild severity category, repeated isoflurane anesthesia ranks higher than single isoflurane anesthesia. Additionally, our results imply that male and female mice can differently perceive the severity of a procedure.", "link"=>"http://www.mendeley.com/research/severity-classification-repeated-isoflurane-anesthesia-c57bl6jrj-mice-assessing-degree-distress", "reader_count"=>17, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>4, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>4, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Nursing and Health Professions"=>1, "Agricultural and Biological Sciences"=>6, "Medicine and Dentistry"=>1, "Veterinary Science and Veterinary Medicine"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Computer Science"=>1, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Computer Science"=>{"Computer Science"=>1}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Unspecified"=>{"Unspecified"=>3}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>3}}, "group_count"=>1}

Scopus | Further Information

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