IGFN1_v1 is required for myoblast fusion and differentiation
Publication Date
June 30, 2017
Authors
Xiang Li, Jane Baker, Tobias Cracknell, Andrew R. Haynes, et al
Volume
12
Issue
6
Pages
e0180217
DOI
https://dx.plos.org/10.1371/journal.pone.0180217
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0180217
Scopus
85021643275
Mendeley
http://www.mendeley.com/research/igfn1v1-required-myoblast-fusion-differentiation
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Mendeley | Further Information

{"title"=>"IGFN1_v1 is required for myoblast fusion and differentiation", "type"=>"journal", "authors"=>[{"first_name"=>"Xiang", "last_name"=>"Li", "scopus_author_id"=>"57194686802"}, {"first_name"=>"Jane", "last_name"=>"Baker", "scopus_author_id"=>"57197523973"}, {"first_name"=>"Tobias", "last_name"=>"Cracknell", "scopus_author_id"=>"57194686796"}, {"first_name"=>"Andrew R.", "last_name"=>"Haynes", "scopus_author_id"=>"57197680958"}, {"first_name"=>"Gonzalo", "last_name"=>"Blanco", "scopus_author_id"=>"7102664019"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1111111111", "issn"=>"19326203", "pmid"=>"28665998", "sgr"=>"85021643275", "pui"=>"617025263", "scopus"=>"2-s2.0-85021643275", "doi"=>"10.1371/journal.pone.0180217"}, "id"=>"2a830773-60cf-3f43-bf26-0c06511fb3fb", "abstract"=>"Igfn1 is a complex locus that codes for multiple splicing variants of Immunoglobulin- and Fibronectin-like domain containing proteins predominantly expressed in skeletal muscle. To reveal possible roles for Igfn1, we applied non-selective knock-down by shRNAs as well as specific targeting of Igfn1 exon 13 by CRISPR/Cas9 mutagenesis in C2C12 cells. Decreased expression of Igfn1 variants via shRNAs against the common 3'-UTR region caused a total blunting of myoblast fusion, but did not prevent expression of differentiation markers. Targeting of N-terminal domains by elimination of exon 13 via CRISPR/Cas9 mediated homologous recombination, also resulted in fusion defects as well as large multinucleated cells. Expression of IGFN1_v1 partially rescued fusion and myotube morphology in the Igfn1 exon 13 knock-out cell line, indicating a role for this variant in myoblast fusion and differentiation. However, in vivo overexpression of IGFN1_v1 or the Igfn1 Exon 13 CRISPR/Cas9 targeting vector did not result in significant size changes in transfected fibres.", "link"=>"http://www.mendeley.com/research/igfn1v1-required-myoblast-fusion-differentiation", "reader_count"=>9, "reader_count_by_academic_status"=>{"Researcher"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Other"=>2, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Researcher"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Other"=>2, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>5, "Agricultural and Biological Sciences"=>3}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>1}

Scopus | Further Information

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