Transcription of putative tonoplast transporters in response to glyphosate and paraquat stress in Conyza bonariensis and Conyza canadensis and selection of reference genes for qRT-PCR
Publication Date
July 10, 2017
Authors
Marcelo L. Moretti, Rocio Alárcon Reverte, Stephen Pearce, Sarah Morran, et al
Volume
12
Issue
7
Pages
e0180794
DOI
https://dx.plos.org/10.1371/journal.pone.0180794
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0180794
Scopus
85022190574
Mendeley
http://www.mendeley.com/research/transcription-putative-tonoplast-transporters-response-glyphosate-paraquat-stress-conyza-bonariensis
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Mendeley | Further Information

{"title"=>"Transcription of putative tonoplast transporters in response to glyphosate and paraquat stress in Conyza bonariensis and Conyza canadensis and selection of reference genes for qRT-PCR", "type"=>"journal", "authors"=>[{"first_name"=>"Marcelo L.", "last_name"=>"Moretti", "scopus_author_id"=>"55005151600"}, {"first_name"=>"Rocio", "last_name"=>"Alárcon-Reverte", "scopus_author_id"=>"26421105700"}, {"first_name"=>"Stephen", "last_name"=>"Pearce", "scopus_author_id"=>"35082549400"}, {"first_name"=>"Sarah", "last_name"=>"Morran", "scopus_author_id"=>"38461430300"}, {"first_name"=>"Bradley D.", "last_name"=>"Hanson", "scopus_author_id"=>"55434293900"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"doi"=>"10.1371/journal.pone.0180794", "isbn"=>"1111111111", "issn"=>"19326203", "pui"=>"617239320", "pmid"=>"28700644", "sgr"=>"85022190574", "scopus"=>"2-s2.0-85022190574"}, "id"=>"8c3782f1-a72c-311e-bcb6-bc581d9a8e8e", "abstract"=>"Herbicide resistance is a challenge for modern agriculture further complicated by cases of resistance to multiple herbicides. Conyza bonariensis and Conyza canadensis are invasive weeds of field crops, orchards, and non-cropped areas in many parts of the world. In California, USA, Conyza populations resistant to the herbicides glyphosate and paraquat have recently been described. Although the mechanism conferring resistance to glyphosate and paraquat in these species was not elucidated, reduced translocation of these herbicides was observed under experimental conditions in both species. Glyphosate and paraquat resistance associated with reduced translocation are hypothesized to be a result of sequestration of herbicides into the vacuole, with the possible involvement of over-expression of genes encoding tonoplast transporters of ABC-transporter families in cases of glyphosate resistance or cationic amino acid transporters (CAT) in cases of paraquat resistance. However, gene expression in response to herbicide treatment has not been studied in glyphosate and paraquat resistant populations. In the current study, we evaluated the transcript levels of genes possibly involved in resistance using real-time PCR. First, we evaluated eight candidate reference genes following herbicide treatment and selected three genes that exhibited stable expression profiles; ACTIN, HEAT-SHOCK-PROTEIN-70, and CYCLOPHILIN. The reference genes identified here can be used for further studies related to plant-herbicide interactions. We used these reference genes to assay the transcript levels of EPSPS, ABC transporters, and CAT in response to herbicide treatment in susceptible and resistant Conyza spp. lines. No transcription changes were observed in EPSPS or CAT genes after glyphosate or paraquat treatment, suggesting that these genes are not involved in the resistance mechanism. Transcription of the two ABC transporter genes increased following glyphosate treatment in all Conyza spp. lines. Transcription of ABC transporters also increased after paraquat treatment in all three lines of C. bonariensis. However, in C. canadensis, paraquat treatment increased transcription of only one ABC transporter gene in the susceptible line. The increase in transcription of ABC transporters after herbicide treatment is likely a stress response based on similar response observed across all Conyza lines regardless of resistance or sensitivity to glyphosate or paraquat, thus these genes do not appear to be directly involved in the mechanism of resistance in Conyza spp. © 2017 Moretti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.", "link"=>"http://www.mendeley.com/research/transcription-putative-tonoplast-transporters-response-glyphosate-paraquat-stress-conyza-bonariensis", "reader_count"=>10, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>2, "Researcher"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>2, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>2, "Researcher"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>2, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Agricultural and Biological Sciences"=>7, "Business, Management and Accounting"=>2}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>1}

Scopus | Further Information

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