Rapid and broad detection of H5 hemagglutinin by an immunochromatographic kit using novel monoclonal antibody against highly pathogenic avian influenza virus belonging to the genetic clade 2.3.4.4
Publication Date
August 07, 2017
Authors
Lam Thanh Nguyen, Kazunari Nakaishi, Keiko Motojima, Ayako Ohkawara, et al
Volume
12
Issue
8
Pages
e0182228
DOI
https://dx.plos.org/10.1371/journal.pone.0182228
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0182228
Scopus
85026881902
Mendeley
http://www.mendeley.com/research/rapid-broad-detection-h5-hemagglutinin-immunochromatographic-kit-using-novel-monoclonal-antibody-aga
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Mendeley | Further Information

{"title"=>"Rapid and broad detection of H5 hemagglutinin by an immunochromatographic kit using novel monoclonal antibody against highly pathogenic avian influenza virus belonging to the genetic clade 2.3.4.4", "type"=>"journal", "authors"=>[{"first_name"=>"Lam Thanh", "last_name"=>"Nguyen", "scopus_author_id"=>"57190425917"}, {"first_name"=>"Kazunari", "last_name"=>"Nakaishi", "scopus_author_id"=>"56403135900"}, {"first_name"=>"Keiko", "last_name"=>"Motojima", "scopus_author_id"=>"57195300463"}, {"first_name"=>"Ayako", "last_name"=>"Ohkawara", "scopus_author_id"=>"56667157300"}, {"first_name"=>"Erina", "last_name"=>"Minato", "scopus_author_id"=>"57195303309"}, {"first_name"=>"Junki", "last_name"=>"Maruyama", "scopus_author_id"=>"55762846500"}, {"first_name"=>"Takahiro", "last_name"=>"Hiono", "scopus_author_id"=>"55576961700"}, {"first_name"=>"Keita", "last_name"=>"Matsuno", "scopus_author_id"=>"16444713300"}, {"first_name"=>"Masatoshi", "last_name"=>"Okamatsu", "scopus_author_id"=>"15077238300"}, {"first_name"=>"Takashi", "last_name"=>"Kimura", "scopus_author_id"=>"55725364800"}, {"first_name"=>"Ayato", "last_name"=>"Takada", "scopus_author_id"=>"7202962935"}, {"first_name"=>"Hiroshi", "last_name"=>"Kida", "scopus_author_id"=>"55573548700"}, {"first_name"=>"Yoshihiro", "last_name"=>"Sakoda", "scopus_author_id"=>"7003600852"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"617681945", "doi"=>"10.1371/journal.pone.0182228", "scopus"=>"2-s2.0-85026881902", "sgr"=>"85026881902", "issn"=>"19326203", "isbn"=>"1111111111"}, "id"=>"52b1d649-e784-3fa3-b150-fef391d81546", "abstract"=>"Highly pathogenic avian influenza viruses (HPAIVs) of H5 subtype have persistently caused outbreaks in domestic poultry and wild birds worldwide and sporadically infected humans. Rapid and accurate diagnosis is one of the key strategies for the control of H5 HPAIVs. However, the sensitivity of the diagnosis of H5 HPAIVs has gradually reduced due to extensive antigenic variation during their evolution. Particularly, the previously developed immunochromatographic diagnosis kit for H5 viruses, Linjudge Flu A/H5, exhibits reduced detection of H5 HPAIVs isolated in recent years. In the present study, we established a new advanced H5 rapid immunochromatographic detection kit (New Linjudge Flu A/H5) by a combination of two anti-H5 hemagglutinin monoclonal antibodies, A64/1 previously applied in the Linjudge Flu A/H5 and A32/2, a novel monoclonal antibody generated from a clade 2.3.4.4 H5 HPAIV. The new kit broadly detected all classical and recent H5 influenza viruses and showed a higher specificity and sensitivity than the original Linjudge Flu A/H5 with recently circulating H5 HPAIVs. Furthermore, the applicability of the New Linjudge Flu A/H5 was demonstrated by detecting antigens from the swabs and tissue homogenates of naturally infected birds and experimentally infected chickens with H5N6 HPAIVs belonging to the genetic clade 2.3.4.4. Our study, therefore, can provide an effective point-of-care rapid antigen detection kit for the surveillance of H5 avian influenza viruses and as a prompt countermeasure against the current widespread of the clade 2.3.4.4 H5 HPAIVs in domestic and wild birds.", "link"=>"http://www.mendeley.com/research/rapid-broad-detection-h5-hemagglutinin-immunochromatographic-kit-using-novel-monoclonal-antibody-aga", "reader_count"=>8, "reader_count_by_academic_status"=>{"Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Environmental Science"=>1, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>4, "Veterinary Science and Veterinary Medicine"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Environmental Science"=>{"Environmental Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>2}}, "group_count"=>1}

Scopus | Further Information

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