Immune characterization of the HBHA-specific response in Mycobacterium tuberculosis-infected patients with or without HIV infection
Publication Date
August 24, 2017
Authors
Teresa Chiacchio, Giovanni Delogu, Valentina Vanini, Gilda Cuzzi, et al
Volume
12
Issue
8
Pages
e0183846
DOI
https://dx.plos.org/10.1371/journal.pone.0183846
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0183846
Scopus
85028413783
Mendeley
http://www.mendeley.com/research/immune-characterization-hbhaspecific-response-mycobacterium-tuberculosisinfected-patients-without-hi
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Mendeley | Further Information

{"title"=>"Immune characterization of the HBHA-specific response in mycobacterium tuberculosis-infected patients with or without HIV infection", "type"=>"journal", "authors"=>[{"first_name"=>"Teresa", "last_name"=>"Chiacchio", "scopus_author_id"=>"35193532700"}, {"first_name"=>"Giovanni", "last_name"=>"Delogu", "scopus_author_id"=>"12800035800"}, {"first_name"=>"Valentina", "last_name"=>"Vanini", "scopus_author_id"=>"35194987100"}, {"first_name"=>"Gilda", "last_name"=>"Cuzzi", "scopus_author_id"=>"23488644800"}, {"first_name"=>"Flavio De", "last_name"=>"Maio", "scopus_author_id"=>"57195483866"}, {"first_name"=>"Carmela", "last_name"=>"Pinnetti", "scopus_author_id"=>"15059521700"}, {"first_name"=>"Alessandro", "last_name"=>"Sampaolesi", "scopus_author_id"=>"6602433693"}, {"first_name"=>"Andrea", "last_name"=>"Antinori", "scopus_author_id"=>"7005824555"}, {"first_name"=>"Delia", "last_name"=>"Goletti", "scopus_author_id"=>"7004262091"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85028413783", "pui"=>"617960521", "doi"=>"10.1371/journal.pone.0183846", "issn"=>"19326203", "scopus"=>"2-s2.0-85028413783", "isbn"=>"1111111111", "pmid"=>"28837654"}, "id"=>"1f7d1672-9068-32d4-b61c-904776b1fcf7", "abstract"=>"INTRODUCTION: RD1-based Interferon-gamma Release Assays (IGRAs) cannot distinguish latent from active tuberculosis (TB) disease. Conversely, a positive response to heparin-binding haemagglutinin (HBHA)-based IGRAs, among TB-infected subjects, correlates with Mycobacterium tuberculosis (Mtb) containment and low risk of TB progression. The aim of this study was to characterize HBHA-immune responses in HIV-infected and uninfected subjects with active TB or latent TB infection (LTBI). METHODS: 49 subjects were prospectively enrolled: 22 HIV-uninfected (13 TB, 9 LTBI) and 27 HIV-infected (12 HIV-TB, 15 HIV-LTBI). Whole blood and peripheral blood mononuclear cells were stimulated with HBHA and RD1 antigens. Interferon (IFN)gamma release was evaluated by ELISA whereas cytokine profile [IFNgamma, tumor necrosis (TNF)alpha, interleukin (IL)2] and phenotype (CD45RA, CCR7) by flow cytometry. RESULTS: Among LTBI individuals, HBHA stimulation induced IFNgamma release in all the HIV-uninfected, while, only 4/15 HIV-infected responded. Within the active TB, only 5/13 HIV-uninfected and 1/12 HIV-TB patients responded. Interestingly, by cytometry we showed that CD4+ T-cells response to HBHA was significantly impaired in the HIV-infected subjects with TB or LTBI compared to the HIV-uninfected subjects. The phenotype of HBHA-specific CD4 T-cells showed a predominantly central memory (CM) and effector memory (EM) phenotype without differences among the groups. Differently, HBHA-specific CD8+ T-cells, showed mainly a CM and naive phenotype in LTBI group while TB, HIV-LTBI and HIV-TB groups were characterized by EM or terminally differentiated phenotypes. Interestingly, differently than what observed for RD1, the cytokine profile of HBHA-specific T-cells evaluated by cytometry showed that the CD4+ T-cells were mostly monofunctional. Conversely, CD8-specific T-cells were mostly monofunctional for both HBHA and RD1 stimulations. CONCLUSIONS: These results characterize the impact of HIV infection in CD4- and CD8-specific response to HBHA in both LTBI and TB patients. HIV infection impairs the CD4 response to HBHA and likely this may lead to an impairment of TB control.", "link"=>"http://www.mendeley.com/research/immune-characterization-hbhaspecific-response-mycobacterium-tuberculosisinfected-patients-without-hi", "reader_count"=>9, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Student > Master"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Nursing and Health Professions"=>1, "Medicine and Dentistry"=>2, "Agricultural and Biological Sciences"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Unspecified"=>{"Unspecified"=>3}}, "group_count"=>0}

Scopus | Further Information

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