Vaccine adjuvant MF59 promotes the intranodal differentiation of antigen-loaded and activated monocyte-derived dendritic cells
Publication Date
October 31, 2017
Authors
Rossella Cioncada, Marcella Maddaluno, Hoa Thi My Vo, Matthew Woodruff, et al
Volume
12
Issue
10
Pages
e0185843
DOI
https://dx.plos.org/10.1371/journal.pone.0185843
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0185843
Scopus
85032887366
Mendeley
http://www.mendeley.com/research/vaccine-adjuvant-mf59-promotes-intranodal-differentiation-antigenloaded-activated-monocytederived-de
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Mendeley | Further Information

{"title"=>"Vaccine adjuvant MF59 promotes the intranodal differentiation of antigen-loaded and activated monocyte-derived dendritic cells", "type"=>"journal", "authors"=>[{"first_name"=>"Rossella", "last_name"=>"Cioncada", "scopus_author_id"=>"55822780300"}, {"first_name"=>"Marcella", "last_name"=>"Maddaluno", "scopus_author_id"=>"35741203500"}, {"first_name"=>"Hoa Thi My", "last_name"=>"Vo", "scopus_author_id"=>"57196409441"}, {"first_name"=>"Matthew", "last_name"=>"Woodruff", "scopus_author_id"=>"37087831800"}, {"first_name"=>"Simona", "last_name"=>"Tavarini", "scopus_author_id"=>"56107498800"}, {"first_name"=>"Chiara", "last_name"=>"Sammicheli", "scopus_author_id"=>"16444591100"}, {"first_name"=>"Marco", "last_name"=>"Tortoli", "scopus_author_id"=>"16235304800"}, {"first_name"=>"Alfredo", "last_name"=>"Pezzicoli", "scopus_author_id"=>"14421308000"}, {"first_name"=>"Ennio", "last_name"=>"De Gregorio", "scopus_author_id"=>"6603054890"}, {"first_name"=>"Michael C.", "last_name"=>"Carroll", "scopus_author_id"=>"7402388322"}, {"first_name"=>"Ugo", "last_name"=>"D’Oro", "scopus_author_id"=>"57078992500"}, {"first_name"=>"Diego", "last_name"=>"Piccioli", "scopus_author_id"=>"6508270386"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-85032887366", "pui"=>"619011462", "issn"=>"19326203", "isbn"=>"1111111111", "doi"=>"10.1371/journal.pone.0185843", "sgr"=>"85032887366"}, "id"=>"860721f4-cb63-36ce-8df0-9b2508091486", "abstract"=>"© 2017 Cioncada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. MF59 is an oil-in-water emulsion adjuvant approved for human influenza vaccination in European Union. The mode of action of MF59 is not fully elucidated yet, but results from several years of investigation indicate that MF59 establishes an immunocompetent environment at injection site which promotes recruitment of immune cells, including antigen presenting cells (APCs), that are facilitated to engulf antigen and transport it to draining lymph node (dLN) where the antigen is accumulated. In vitro studies showed that MF59 promotes the differentiation of monocytes to dendritic cells (Mo-DCs). Since after immunization with MF59, monocytes are rapidly recruited both at the injection site and in dLN and appear to have a morphological change toward a DC-like phenotype, we asked whether MF59 could play a role in inducing differentiation of Mo-DC in vivo. To address this question we immunized mice with the auto-fluorescent protein Phycoerythrin (PE) as model antigen, in presence or absence of MF59. We measured the APC phenotype and their antigen uptake within dLNs, the antigen distribution within the dLN compartments and the humoral response to PE. In addition, using Ovalbumin as model antigen, we measured the capacity of dLN APCs to induce antigen-specific CD4 T cell proliferation. Here, we show, for the first time, that MF59 promotes differentiation of Mo-DCs within dLNs from intranodal recruited monocytes and we suggest that this differentiation could take place in the medullary compartment of the LN. In addition we show that the Mo-DC subset represents the major source of antigen-loaded and activated APCs within the dLN when immunizing with MF59. Interestingly, this finding correlates with the enhanced triggering of antigen-specific CD4 T cell response induced by LN APCs. This study therefore demonstrates that MF59 is able to promote an immunocompetent environment also directly within the dLN, offering a novel insight on the mechanism of action of vaccine adjuvants based on emulsions.", "link"=>"http://www.mendeley.com/research/vaccine-adjuvant-mf59-promotes-intranodal-differentiation-antigenloaded-activated-monocytederived-de", "reader_count"=>3, "reader_count_by_academic_status"=>{"Researcher"=>1, "Student > Ph. D. Student"=>2}, "reader_count_by_user_role"=>{"Researcher"=>1, "Student > Ph. D. Student"=>2}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}}, "group_count"=>0}

Scopus | Further Information

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