Human adipose-derived stem cells support the growth of limbal stem/progenitor cells
Publication Date
October 11, 2017
Authors
Hua Mei, Sheyla González, Martin N. Nakatsu, Elfren R. Baclagon, et al
Volume
12
Issue
10
Pages
e0186238
DOI
https://dx.plos.org/10.1371/journal.pone.0186238
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0186238
Scopus
85030973854
Mendeley
http://www.mendeley.com/research/human-adiposederived-stem-cells-support-growth-limbal-stemprogenitor-cells
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Mendeley | Further Information

{"title"=>"Human adipose-derived stem cells support the growth of limbal stem/progenitor cells", "type"=>"journal", "authors"=>[{"first_name"=>"Hua", "last_name"=>"Mei", "scopus_author_id"=>"36559791000"}, {"first_name"=>"Sheyla", "last_name"=>"González", "scopus_author_id"=>"55885759400"}, {"first_name"=>"Martin N.", "last_name"=>"Nakatsu", "scopus_author_id"=>"8775271200"}, {"first_name"=>"Elfren R.", "last_name"=>"Baclagon", "scopus_author_id"=>"56071607900"}, {"first_name"=>"Felix V.", "last_name"=>"Chen", "scopus_author_id"=>"57196023083"}, {"first_name"=>"Sophie X.", "last_name"=>"Deng", "scopus_author_id"=>"7202438017"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"618698377", "sgr"=>"85030973854", "issn"=>"19326203", "pmid"=>"29020119", "scopus"=>"2-s2.0-85030973854", "doi"=>"10.1371/journal.pone.0186238", "isbn"=>"1111111111"}, "id"=>"102288f6-5e0e-3e9c-856a-7bb99d7f66ab", "abstract"=>"The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro. To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). The expanded LSCs were examined at the end of a 2-week culture. The standard 3T3 culture served as control. Expansion of SC-ASC showed limited proliferation and exhibited differentiated morphology. CC-ASC generated epithelial cells with undifferentiated morphology in all culture methods, among which CC-ASC in 3D culture supported the highest cell doubling (cells doubled 9.0 times compared to cells doubled 4.9 times in control) while maintained the percentage of putative limbal stem/progenitor cells compared to the control. There were few cell-cell contacts between cultured LSCs and ASCs in 3D CC-ASC. In conclusion, ASCs support the growth of LSCs in the form of cell clusters but not in single cells. 3D CC-ASC could serve as a substitute for the standard 3T3 culture to expand LSCs.", "link"=>"http://www.mendeley.com/research/human-adiposederived-stem-cells-support-growth-limbal-stemprogenitor-cells", "reader_count"=>3, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Medicine and Dentistry"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "group_count"=>0}

Scopus | Further Information

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