Screening for HIV, hepatitis B and syphilis on dried blood spots: A promising method to better reach hidden high-risk populations with self-collected sampling
Publication Date
October 20, 2017
Authors
Inge H. M. Van Loo, Nicole H. T. M. Dukers Muijrers, Rosalie Heuts, Marianne A. B. Van Der Sande, et al
Volume
12
Issue
10
Pages
e0186722
DOI
https://dx.plos.org/10.1371/journal.pone.0186722
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0186722
Scopus
85031784650
Mendeley
http://www.mendeley.com/research/screening-hiv-hepatitis-b-syphilis-dried-blood-spots-promising-method-better-reach-hidden-highrisk-p
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Mendeley | Further Information

{"title"=>"Screening for HIV, hepatitis B and syphilis on dried blood spots: A promising method to better reach hidden high-risk populations with self-collected sampling", "type"=>"journal", "authors"=>[{"first_name"=>"Inge H.M.", "last_name"=>"Van Loo", "scopus_author_id"=>"6508021715"}, {"first_name"=>"Nicole H.T.M.", "last_name"=>"Dukers-Muijrers", "scopus_author_id"=>"25421482000"}, {"first_name"=>"Rosalie", "last_name"=>"Heuts", "scopus_author_id"=>"57196089766"}, {"first_name"=>"Marianne A.B.", "last_name"=>"Van Der Sande", "scopus_author_id"=>"16403677800"}, {"first_name"=>"Christian J.P.A.", "last_name"=>"Hoebe", "scopus_author_id"=>"6701736346"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "doi"=>"10.1371/journal.pone.0186722", "sgr"=>"85031784650", "scopus"=>"2-s2.0-85031784650", "isbn"=>"1111111111", "pmid"=>"29053737", "pui"=>"618815684"}, "id"=>"2791cc9e-769d-321d-95b0-acc00d9adf22", "abstract"=>"INTRODUCTION: Many people at high risk for sexually transmitted infections (STIs), e.g., men who have sex with men (MSM), are not optimally reached by current sexual health care systems with testing. To facilitate testing by home-based sampling or sampling in outreach setting we evaluated dried blood spots (DBS), a method for self-collected blood sampling for serological screening of HIV, hepatitis B (HBV) and syphilis. The aims of this study were to assess the acceptability and feasibility of self-collected DBS and to compare the test results for screening of HIV, HBV and syphilis from DBS with blood drawn by venous puncture. METHODS: DBS were collected from men who have sex with men (MSM), visiting the STI clinic of the public health service South Limburg (n = 183) and HIV positive and HBV positive patients (n = 34), visiting the outpatient clinics of the Maastricht University Medical Centre in the period January 2012-April 2015. The 93 first participating MSM visiting the STI clinic were asked to fill in a questionnaire about the feasibility and acceptability about self-collection of DBS in a setting without going to a health care facility and were asked to collect the DBS themselves. Serological screening tests for HIV (HIV combi PT, Roche), HBV (HBsAg, Roche) and syphilis (Treponema pallidum Ig, Biokit 3.0) were performed on DBS and on blood drawn by venous puncture, which was routinely taken for screening. RESULTS: In total 217 participants were included in the study with a median age of 40 years (range between 17-80). Of MSM 84% agreed that it was clear and easy to do the finger-prick, while 53% agreed that it was clear and easy to apply the blood onto the DBS card. Also, 80% of MSM would use the bloodspot test again. In 91% (198) of DBS, sufficient material was collected to perform the three tests. No difference was observed in DBS quality between self-collected DBS and health care worker collected DBS. For HIV (n = 195 DBS-serum pairs) sensitivity and specificity were 100%. For HBV the sensitivity for HBsAg (n = 202) was 90% and specificity was 99%. For syphilis (n = 191) the sensitivity of the DBS was 93% with a specificity of 99%. Analysis of the DBS of HIV positive participants (n = 38) did show similar test performance for HBV and syphilis as in HIV negatives. CONCLUSION: DBS is an acceptable self-sampling method for MSM, as there was no difference in DBS quality in self-collected and health care worker collected DBS. Test performance, i.e., its high sensitivity (>90%) and specificity (>99%) measures show that DBS is a valid alternative for venous blood puncture. Especially when DBS is combined with home-collected sampling for Chlamydia trachomatis and Neisseria gonorrhoeae, complete STI screening can be done in outreach setting and/or home-collected sampling in MSM.", "link"=>"http://www.mendeley.com/research/screening-hiv-hepatitis-b-syphilis-dried-blood-spots-promising-method-better-reach-hidden-highrisk-p", "reader_count"=>9, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Postgraduate"=>1, "Student > Bachelor"=>1, "Student > Ph. D. Student"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>3, "Student > Doctoral Student"=>2, "Student > Postgraduate"=>1, "Student > Bachelor"=>1, "Student > Ph. D. Student"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>1, "Medicine and Dentistry"=>3, "Arts and Humanities"=>1, "Psychology"=>1, "Business, Management and Accounting"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "group_count"=>1}

Scopus | Further Information

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