Construction of an infectious horsepox virus vaccine from chemically synthesized DNA fragments
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{"title"=>"Construction of an infectious horsepox virus vaccine from chemically synthesized DNA fragments", "type"=>"journal", "authors"=>[{"first_name"=>"Ryan S.", "last_name"=>"Noyce", "scopus_author_id"=>"8767720800"}, {"first_name"=>"Seth", "last_name"=>"Lederman", "scopus_author_id"=>"57200327676"}, {"first_name"=>"David H.", "last_name"=>"Evans", "scopus_author_id"=>"57199095681"}], "year"=>2018, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "isbn"=>"1111111111", "pmid"=>"29351298", "scopus"=>"2-s2.0-85040865656", "pui"=>"620255981", "doi"=>"10.1371/journal.pone.0188453", "sgr"=>"85040865656"}, "id"=>"14ab482e-b446-3477-8fd9-6c25dbdd96bd", "abstract"=>"Edward Jenner and his contemporaries believed that his variolae vaccinae originated in horses and molecular analyses show that modern vaccinia virus (VACV) strains share common ancestry with horsepox virus (HPXV). Given concerns relating to the toxicity of modern VACV vaccines, we asked whether an HPXV-based vaccine might provide a superior alternative. Since HPXV may be extinct and the only specimen of HPXV that has been identified is unavailable for investigation, we explored whether HPXV could be obtained by large-scale gene synthesis. Ten large (10–30 kb) fragments of DNA were synthesized based on the HPXV sequence along with two 157 nt VACV terminal sequences, and were recombined into a live synthetic chimeric HPXV (scHPXV) in cells infected with Shope fibroma virus (SFV). Sequencing of the 212 kbp scHPXV confirmed it encoded a faithful copy of the input DNA. We believe this is the first complete synthesis of a poxvirus using synthetic biology approaches. This scHPXV produced smaller plaques, produced less extracellular virus and exhibited less virulence in mice than VACV, but still provided vaccine protection against a lethal VACV challenge. Collectively, these findings support further development of scHPXV as a novel replication-proficient smallpox vaccine.", "link"=>"http://www.mendeley.com/research/construction-infectious-horsepox-virus-vaccine-chemically-synthesized-dna-fragments", "reader_count"=>47, "reader_count_by_academic_status"=>{"Unspecified"=>5, "Professor > Associate Professor"=>3, "Researcher"=>7, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>2, "Other"=>4, "Student > Master"=>9, "Student > Bachelor"=>7}, "reader_count_by_user_role"=>{"Unspecified"=>5, "Professor > Associate Professor"=>3, "Researcher"=>7, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>8, "Student > Postgraduate"=>2, "Other"=>4, "Student > Master"=>9, "Student > Bachelor"=>7}, "reader_count_by_subject_area"=>{"Engineering"=>2, "Unspecified"=>10, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>13, "Materials Science"=>1, "Agricultural and Biological Sciences"=>7, "Medicine and Dentistry"=>1, "Neuroscience"=>1, "Chemical Engineering"=>1, "Chemistry"=>2, "Computer Science"=>2, "Immunology and Microbiology"=>4, "Arts and Humanities"=>2}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Unspecified"=>{"Unspecified"=>10}, "Environmental Science"=>{"Environmental Science"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Arts and Humanities"=>{"Arts and Humanities"=>2}, "Engineering"=>{"Engineering"=>2}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Computer Science"=>{"Computer Science"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>13}}, "group_count"=>1}

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