Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo
Publication Date
December 13, 2017
Authors
Peter Apelgren, Matteo Amoroso, Anders Lindahl, Camilla Brantsing, et al
Volume
12
Issue
12
Pages
e0189428
DOI
https://dx.plos.org/10.1371/journal.pone.0189428
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0189428
Scopus
85038222884
Mendeley
http://www.mendeley.com/research/chondrocytes-stem-cells-3dbioprinted-structures-create-human-cartilage-vivo
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Mendeley | Further Information

{"title"=>"Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo", "type"=>"journal", "authors"=>[{"first_name"=>"Peter", "last_name"=>"Apelgren", "scopus_author_id"=>"15724406200"}, {"first_name"=>"Matteo", "last_name"=>"Amoroso", "scopus_author_id"=>"55778448500"}, {"first_name"=>"Anders", "last_name"=>"Lindahl", "scopus_author_id"=>"35508512900"}, {"first_name"=>"Camilla", "last_name"=>"Brantsing", "scopus_author_id"=>"6505913489"}, {"first_name"=>"Nicole", "last_name"=>"Rotter", "scopus_author_id"=>"6603710550"}, {"first_name"=>"Paul", "last_name"=>"Gatenholm", "scopus_author_id"=>"7006195365"}, {"first_name"=>"Lars", "last_name"=>"Kölby", "scopus_author_id"=>"7003748686"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85038222884", "doi"=>"10.1371/journal.pone.0189428", "pui"=>"619746203", "pmid"=>"29236765", "scopus"=>"2-s2.0-85038222884", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"3b22fb07-a7d7-38dc-929d-86354e92cc88", "abstract"=>"Cartilage repair and replacement is a major challenge in plastic reconstructive surgery. The development of a process capable of creating a patient-specific cartilage framework would be a major breakthrough. Here, we described methods for creating human cartilage in vivo and quantitatively assessing the proliferative capacity and cartilage-formation ability in mono- and co-cultures of human chondrocytes and human mesenchymal stem cells in a three-dimensional (3D)-bioprinted hydrogel scaffold. The 3D-bioprinted constructs (5 × 5 × 1.2 mm) were produced using nanofibrillated cellulose and alginate in combination with human chondrocytes and human mesenchymal stem cells using a 3D-extrusion bioprinter. Immediately following bioprinting, the constructs were implanted subcutaneously on the back of 48 nude mice and explanted after 30 and 60 days, respectively, for morphological and immunohistochemical examination. During explantation, the constructs were easy to handle, and the majority had retained their macroscopic grid appearance. Constructs con- sisting of human nasal chondrocytes showed good proliferation ability, with 17.2% of the surface areas covered with proliferating chondrocytes after 60 days. In constructs compris- ing a mixture of chondrocytes and stem cells, an additional proliferative effect was observed involving chondrocyte production of glycosaminoglycans and type 2 collagen. This clinically highly relevant study revealed 3D bioprinting as a promising technology for the creation of human cartilage.", "link"=>"http://www.mendeley.com/research/chondrocytes-stem-cells-3dbioprinted-structures-create-human-cartilage-vivo", "reader_count"=>20, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>6, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Materials Science"=>1, "Medicine and Dentistry"=>3, "Agricultural and Biological Sciences"=>2, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>6}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Social Sciences"=>{"Social Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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