Profiles of pro-opiomelanocortin and encoded peptides, and their processing enzymes in equine pituitary pars intermedia dysfunction
Publication Date
January 08, 2018
Authors
James L. Carmalt, Sima Mortazavi, Rebecca C. Mc Onie, Andrew L. Allen, et al
Volume
13
Issue
1
Pages
e0190796
DOI
https://dx.plos.org/10.1371/journal.pone.0190796
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0190796
Scopus
85040379060
Mendeley
http://www.mendeley.com/research/profiles-proopiomelanocortin-encoded-peptides-processing-enzymes-equine-pituitary-pars-intermedia-dy
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Mendeley | Further Information

{"title"=>"Profiles of pro-opiomelanocortin and encoded peptides, and their processing enzymes in equine pituitary pars intermedia dysfunction", "type"=>"journal", "authors"=>[{"first_name"=>"James L.", "last_name"=>"Carmalt", "scopus_author_id"=>"6603908067"}, {"first_name"=>"Sima", "last_name"=>"Mortazavi", "scopus_author_id"=>"56450314100"}, {"first_name"=>"Rebecca C.", "last_name"=>"McOnie", "scopus_author_id"=>"57194641819"}, {"first_name"=>"Andrew L.", "last_name"=>"Allen", "scopus_author_id"=>"7402802689"}, {"first_name"=>"Suraj", "last_name"=>"Unniappan", "scopus_author_id"=>"14627792100"}], "year"=>2018, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85040379060", "doi"=>"10.1371/journal.pone.0190796", "scopus"=>"2-s2.0-85040379060", "pui"=>"620133167", "issn"=>"19326203"}, "id"=>"6a02742f-35aa-333c-bc56-5870f2346f9f", "abstract"=>"© 2018 Carmalt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Equine pituitary pars intermedia dysfunction (PPID) is characterized by hyperplasia of the pars intermedia (PI) melanotrophs of the pituitary gland (PG), and increased production of proopiomelanocortin (POMC). POMC is cleaved by prohormone convertase 1 (PC1) to produce adrenocorticotropic hormone (ACTH), and further processing of ACTH by PC2 to produce alpha-melanocyte stimulating hormone (α-MSH) and corticotropin-like intermediate peptide (CLIP). High plasma ACTH concentrations in horses with PPID might be related to reduced conversion of ACTH to α-MSH by PCs. The hypothesis of this study was that PC1 and PC2 expression in the pituitary gland are altered in PPID, resulting in an abnormal relative abundance of POMC derived proteins. The objectives of this study were to identify the partial sequences of equine POMC, PC1, and PC2 mRNAs; and to determine whether the expression of POMC, PC1, and PC2 mRNAs in whole pituitary extracts, and POMC-protein in the cavernous sinus blood of horses are altered in PPID. We confirmed (RT-PCR and sequencing) that the partial sequences obtained match the corresponding regions of predicted equine POMC, PC1 and PC2 sequences. The expression (quantificat ion by RT-qPCR) of POMC, PC1 and PC2 mRNAs were found upregulated in the pituitary of horses with PPID. Plasma (measured using RIA/ELISA) ACTH and α-MSH were elevated in PPID horses. These results indicate distinct differences in gene and protein expression of POMC and its intermediates, and processing enzymes in PPID. It provides evidence to support the notion that local, pituitary-specific inadequacies in prohormone processing likely contribute to equine PPID.", "link"=>"http://www.mendeley.com/research/profiles-proopiomelanocortin-encoded-peptides-processing-enzymes-equine-pituitary-pars-intermedia-dy", "reader_count"=>2, "reader_count_by_academic_status"=>{"Professor"=>2}, "reader_count_by_user_role"=>{"Professor"=>2}, "reader_count_by_subject_area"=>{"Medicine and Dentistry"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}}, "group_count"=>0}

Scopus | Further Information

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