Induction of epigenetic variation in Arabidopsis by over-expression of DNA METHYLTRANSFERASE1 (MET1)
Publication Date
February 21, 2018
Authors
Samuel Brocklehurst, Michael Watson, Ian M. Carr, Suzan Out, et al
Volume
13
Issue
2
Pages
e0192170
DOI
https://dx.plos.org/10.1371/journal.pone.0192170
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0192170
Scopus
85042383360
Mendeley
http://www.mendeley.com/research/induction-epigenetic-variation-arabidopsis-overexpression-dna-methyltransferase1-met1
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Mendeley | Further Information

{"title"=>"Induction of epigenetic variation in Arabidopsis by over-expression of DNA METHYLTRANSFERASE1 (MET1)", "type"=>"journal", "authors"=>[{"first_name"=>"Samuel", "last_name"=>"Brocklehurst", "scopus_author_id"=>"57200758378"}, {"first_name"=>"Michael", "last_name"=>"Watson", "scopus_author_id"=>"56657079500"}, {"first_name"=>"Ian M.", "last_name"=>"Carr", "scopus_author_id"=>"7005288760"}, {"first_name"=>"Suzan", "last_name"=>"Out", "scopus_author_id"=>"57200757715"}, {"first_name"=>"Iris", "last_name"=>"Heidmann", "scopus_author_id"=>"6603254157"}, {"first_name"=>"Peter", "last_name"=>"Meyer", "scopus_author_id"=>"7402019055"}], "year"=>2018, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-85042383360", "sgr"=>"85042383360", "doi"=>"10.1371/journal.pone.0192170", "pui"=>"620772479", "issn"=>"19326203", "isbn"=>"1111111111"}, "id"=>"311c63f1-9fcc-30ab-ac4c-21927162ebdd", "abstract"=>"© 2018 Brocklehurst et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited . Epigenetic marks such as DNA methylation and histone modification can vary among plant accessions creating epi-alleles with different levels of expression competence. Mutations in epigenetic pathway functions are powerful tools to induce epigenetic variation. As an alternative approach, we investigated the potential of over-expressing an epigenetic function, using DNA METHYLTRANSFERASE1 (MET1) for proof-of-concept. In Arabidopsis thaliana, MET1 controls maintenance of cytosine methylation at symmetrical CG positions. At some loci, which contain dense DNA methylation in CG- and non-CG context, loss of MET1 causes joint loss of all cytosines methylation marks. We find that over-expression of both catalytically active and inactive versions of MET1 stochastically generates new epi-alleles at loci encoding transposable elements, non-coding RNAs and proteins, which results for most loci in an increase in expression. Individual transformants share some common phenotypes and genes with altered gene expression. Altered expression states can be transmitted to the next generation, which does not require the continuous presence of the MET1 transgene. Long-term stability and epigenetic features differ for individual loci. Our data show that overexpression of MET1, and potentially of other genes encoding epigenetic factors, offers an alternative strategy to identify epigenetic target genes and to create novel epi-alleles.", "link"=>"http://www.mendeley.com/research/induction-epigenetic-variation-arabidopsis-overexpression-dna-methyltransferase1-met1", "reader_count"=>7, "reader_count_by_academic_status"=>{"Student > Ph. D. Student"=>3, "Other"=>1, "Student > Bachelor"=>2, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Student > Ph. D. Student"=>3, "Other"=>1, "Student > Bachelor"=>2, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>4, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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