Identification of plasma biomarkers for discrimination between tuberculosis infection/disease and pulmonary non tuberculosis disease
Publication Date
March 15, 2018
Authors
Marco Pio La Manna, Valentina Orlando, Paolo Li Donni, Guido Sireci, et al
Volume
13
Issue
3
Pages
e0192664
DOI
https://dx.plos.org/10.1371/journal.pone.0192664
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0192664
Scopus
85044134411
Mendeley
http://www.mendeley.com/research/identification-plasma-biomarkers-discrimination-between-tuberculosis-infectiondisease-pulmonary-non
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Mendeley | Further Information

{"title"=>"Identification of plasma biomarkers for discrimination between tuberculosis infection/disease and pulmonary non tuberculosis disease", "type"=>"journal", "authors"=>[{"first_name"=>"Marco Pio", "last_name"=>"La Manna", "scopus_author_id"=>"14037622400"}, {"first_name"=>"Valentina", "last_name"=>"Orlando", "scopus_author_id"=>"16033524500"}, {"first_name"=>"Paolo", "last_name"=>"Li Donni", "scopus_author_id"=>"55014652300"}, {"first_name"=>"Guido", "last_name"=>"Sireci", "scopus_author_id"=>"57195257187"}, {"first_name"=>"Paola", "last_name"=>"Di Carlo", "scopus_author_id"=>"6604018676"}, {"first_name"=>"Antonio", "last_name"=>"Cascio", "scopus_author_id"=>"7006353259"}, {"first_name"=>"Francesco", "last_name"=>"Dieli", "scopus_author_id"=>"57079709600"}, {"first_name"=>"Nadia", "last_name"=>"Caccamo", "scopus_author_id"=>"6602186373"}], "year"=>2018, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-85044134411", "sgr"=>"85044134411", "doi"=>"10.1371/journal.pone.0192664", "pui"=>"621241375", "pmid"=>"29543810", "isbn"=>"1932-6203", "issn"=>"19326203"}, "id"=>"13d1e7e1-4397-329a-864b-47ba5f4d1bc3", "abstract"=>"We used the Luminex Bead Array Multiplex Immunoassay to measure cytokines, chemokines and growth factors responses to the same antigens used for RD1-based Interferon γ Release Assay (IGRA) test. Seventy-nine individuals, 27 active TB, 32 latent infection subsets, 20 individuals derivative purified protein (PPD) negative (subjects that do not have any indurative cutaneous reaction after 72 hrs of intradermal injection of PPD) and with other pulmonary disease were retrospectively studied. Forty-eight analytes were evaluated by Luminex Assay in plasma obtained from whole blood stimulated cells. The diagnostic accuracies of the markers detected were evaluated by ROC curve analysis and by the combination of multiple biomarkers to improve the potential to discriminate between infection/disease and non infection. Among 48 cytokines, 13 analytes, namely IL-3, IL-12-p40, LIF, IFNα2, IL-2ra, IL-13, b-NGF, SCF, TNF-β, TRAIL, IL-2, IFN-γ, IP-10, and MIG, were significantly higher in the active TB and LTBI groups, compared to NON-TB patients, while MIF was significantly lower in active TB patients compared to NON-TB and LTBI groups. The diagnostic accuracies of the markers detected in the culture supernatants evaluated by ROC curve analysis revealed that 11 analytes (IL2, IP10, IFN-γ, IL13, MIG, SCF, b-NGF, IL12-p40, TRAIL, IL2 Ra, LIF) discriminated between NON-TB and LTBI groups, with AUC for all analytes ≥0.73, while 14 analytes (IL2, IP10, IFN-γ, MIG, SCF, b-NGF, IL12-p40, TRAIL, IL2Ra, MIF, TNF-β, IL3, IFN-α2, LIF) discriminated between NON-TB and active TB groups, with AUC ≥0.78, that is a moderate, value in terms of accuracy of a diagnostic test. Finally, the combinations of seven biomarkers resulted in the accurate prediction of 88.89% of active TB patients, 82.35% of subjects with latent infection and 90% of non-TB patients, respectively. Taken together, our data suggest that combinations of whole blood Mycobacterium tuberculosis (Mtb) antigen dependent cytokines production could be useful as biomarkers to determine tuberculosis disease states when compared to non TB cohort.", "link"=>"http://www.mendeley.com/research/identification-plasma-biomarkers-discrimination-between-tuberculosis-infectiondisease-pulmonary-non", "reader_count"=>6, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Other"=>1, "Professor"=>1, "Researcher"=>1, "Student > Postgraduate"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Other"=>1, "Professor"=>1, "Researcher"=>1, "Student > Postgraduate"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Medicine and Dentistry"=>2, "Immunology and Microbiology"=>1, "Agricultural and Biological Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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