Chromosomal distribution of pTa-535, pTa-86, pTa-713, 35S rDNA repetitive sequences in interspecific hexaploid hybrids of common wheat (Triticum aestivum L.) and spelt (Triticum spelta L.)
Publication Date
February 15, 2018
Authors
Klaudia Goriewa Duba, Adrian Duba, Michał Kwiatek, Halina Wiśniewska, et al
Volume
13
Issue
2
Pages
e0192862
DOI
https://dx.plos.org/10.1371/journal.pone.0192862
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0192862
Scopus
85042181455
Mendeley
http://www.mendeley.com/research/chromosomal-distribution-pta535-pta86-pta713-35s-rdna-repetitive-sequences-interspecific-hexaploid-h
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Mendeley | Further Information

{"title"=>"Chromosomal distribution of pTa-535, pTa-86, pTa-713, 35S rDNA repetitive sequences in interspecific hexaploid hybrids of common wheat (Triticum aestivum L.) and spelt (Triticum spelta L.)", "type"=>"journal", "authors"=>[{"first_name"=>"Klaudia", "last_name"=>"Goriewa-Duba", "scopus_author_id"=>"57200685591"}, {"first_name"=>"Adrian", "last_name"=>"Duba", "scopus_author_id"=>"57191249214"}, {"first_name"=>"Michał", "last_name"=>"Kwiatek", "scopus_author_id"=>"54923250200"}, {"first_name"=>"Halina", "last_name"=>"Wiśniewska", "scopus_author_id"=>"6701578724"}, {"first_name"=>"Urszula", "last_name"=>"Wachowska", "scopus_author_id"=>"6506065921"}, {"first_name"=>"Marian", "last_name"=>"Wiwart", "scopus_author_id"=>"56539766800"}], "year"=>2018, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1111111111", "doi"=>"10.1371/journal.pone.0192862", "pui"=>"620727259", "issn"=>"19326203", "sgr"=>"85042181455", "scopus"=>"2-s2.0-85042181455"}, "id"=>"fa7d0621-42c9-342b-9f75-119f22658507", "abstract"=>"Copyright: © 2018 Goriewa-Duba et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fluorescent in situ hybridization (FISH) relies on fluorescent-labeled probes to detect specific DNA sequences in the genome, and it is widely used in cytogenetic analyses. The aim of this study was to determine the karyotype of T. aestivum and T. spelta hybrids and their parental components (three common wheat cultivars and five spelt breeding lines), to identify chromosomal aberrations in the evaluated wheat lines, and to analyze the distribution of polymorphisms of repetitive sequences in the examined hybrids. The FISH procedure was carried out with four DNA clones, pTa-86, pTa-535, pTa-713 and 35S rDNA used as probes. The observed polymorphisms between the investigated lines of common wheat, spelt and their hybrids was relatively low. However, differences were observed in the distribution of repetitiv e sequences on chromosomes 4A, 6A, 1B and 6B in selected hybrid genomes. The polymorphisms observed in common wheat and spelt hybrids carry valuable information for wheat breeders. The results of our study are also a valuable source of knowledge about genome organization and diversification in common wheat, spelt and their hybrids. The relevant information is essential for common wheat breeders, and it can contribute to breeding programs aimed at biodiversity preservation.", "link"=>"http://www.mendeley.com/research/chromosomal-distribution-pta535-pta86-pta713-35s-rdna-repetitive-sequences-interspecific-hexaploid-h", "reader_count"=>4, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Ph. D. Student"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Ph. D. Student"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>2, "Veterinary Science and Veterinary Medicine"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>1}

Scopus | Further Information

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