Transcriptome-wide selection of a reliable set of reference genes for gene expression studies in potato cyst nematodes (Globodera spp.)
Publication Date
March 02, 2018
Authors
Michael Sabeh, Marc Olivier Duceppe, Marc St Arnaud & Benjamin Mimee
Volume
13
Issue
3
Pages
e0193840
DOI
https://dx.plos.org/10.1371/journal.pone.0193840
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0193840
Scopus
85042921767
Mendeley
http://www.mendeley.com/research/transcriptomewide-selection-reliable-set-reference-genes-gene-expression-studies-potato-cyst-nematod
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Mendeley | Further Information

{"title"=>"Transcriptome-wide selection of a reliable set of reference genes for gene expression studies in potato cyst nematodes (globodera spp.)", "type"=>"journal", "authors"=>[{"first_name"=>"Michael", "last_name"=>"Sabeh", "scopus_author_id"=>"57189635798"}, {"first_name"=>"Marc Olivier", "last_name"=>"Duceppe", "scopus_author_id"=>"24471043900"}, {"first_name"=>"Marc", "last_name"=>"St-Arnaud", "scopus_author_id"=>"6603996098"}, {"first_name"=>"Benjamin", "last_name"=>"Mimee", "scopus_author_id"=>"8265481100"}], "year"=>2018, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"85042921767", "isbn"=>"1111111111", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0193840", "scopus"=>"2-s2.0-85042921767", "pui"=>"620929216"}, "id"=>"3d9bf101-9ef7-3472-9f8a-cc3c77f1fc96", "abstract"=>"\r\n\r\nRelative gene expression analyses by qRT-PCR (quantitative reverse transcription PCR) require an internal control to normalize the expression data of genes of interest and eliminate the unwanted variation introduced by sample preparation. A perfect reference gene should have a constant expression level under all the experimental conditions. However, the same few housekeeping genes selected from the literature or successfully used in previous unrelated experiments are often routinely used in new conditions without proper validation of their stability across treatments. The advent of RNA-Seq and the availability of public datasets for numerous organisms are opening the way to finding better reference genes for expression studies. <i>Globodera rostochiensis</i> is a plant-parasitic nematode that is particularly yield-limiting for potato. The aim of our study was to identify a reliable set of reference genes to study <i>G</i>. <i>rostochiensis</i> gene expression. Gene expression levels from an RNA-Seq database were used to identify putative reference genes and were validated with qRT-PCR analysis. Three genes, <i>GR</i>, <i>PMP-3</i>, and <i>aaRS</i>, were found to be very stable within the experimental conditions of this study and are proposed as reference genes for future work.", "link"=>"http://www.mendeley.com/research/transcriptomewide-selection-reliable-set-reference-genes-gene-expression-studies-potato-cyst-nematod", "reader_count"=>3, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Ph. D. Student"=>1, "Researcher"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Ph. D. Student"=>1, "Researcher"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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