Evidence of functional cell-mediated immune responses to nontypeable Haemophilus influenzae in otitis-prone children
Publication Date
April 05, 2018
Authors
Elke Seppanen, Dino Tan, Karli J. Corscadden, Andrew J. Currie, et al
Volume
13
Issue
4
Pages
e0193962
DOI
https://dx.plos.org/10.1371/journal.pone.0193962
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0193962
Scopus
85045067810
Mendeley
http://www.mendeley.com/research/evidence-functional-cellmediated-immune-responses-nontypeable-haemophilus-influenzae-otitisprone-chi
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Mendeley | Further Information

{"title"=>"Evidence of functional cell-mediated immune responses to nontypeable Haemophilus influenzae in otitis-prone children", "type"=>"journal", "authors"=>[{"first_name"=>"Elke", "last_name"=>"Seppanen", "scopus_author_id"=>"36599406500"}, {"first_name"=>"Dino", "last_name"=>"Tan", "scopus_author_id"=>"57201479277"}, {"first_name"=>"Karli J.", "last_name"=>"Corscadden", "scopus_author_id"=>"38861454400"}, {"first_name"=>"Andrew J.", "last_name"=>"Currie", "scopus_author_id"=>"26532356200"}, {"first_name"=>"Peter C.", "last_name"=>"Richmond", "scopus_author_id"=>"7006528644"}, {"first_name"=>"Ruth B.", "last_name"=>"Thornton", "scopus_author_id"=>"21234642500"}, {"first_name"=>"Lea Ann S.", "last_name"=>"Kirkham", "scopus_author_id"=>"10540851700"}], "year"=>2018, "source"=>"PLoS ONE", "identifiers"=>{"doi"=>"10.1371/journal.pone.0193962", "sgr"=>"85045067810", "pui"=>"621571383", "scopus"=>"2-s2.0-85045067810", "issn"=>"19326203", "pmid"=>"29621281", "isbn"=>"1111111111"}, "id"=>"f5274fb2-a535-3538-bfca-2a6501bcef13", "abstract"=>"Otitis media (OM) remains a common paediatric disease, despite advances in vaccinology. Susceptibility to recurrent acute OM (rAOM) has been postulated to involve defective cell-mediated immune responses to common otopathogenic bacteria. We compared the composition of peripheral blood mononuclear cells (PBMC) from 20 children with a history of rAOM (otitis-prone) and 20 healthy non-otitis-prone controls, and assessed innate and cell-mediated immune responses to the major otopathogen nontypeable Haemophilus influenzae (NTHi). NTHi was a potent stimulator of inflammatory cytokine secretion from PBMC within 4 hours, with no difference in cytokine levels produced between PBMC from cases or controls. In the absence of antigen stimulation, otitis-prone children had more circulating Natural Killer (NK) cells (p<0.01), particularly NKdim (CD56lo) cells (p<0.01), but fewer CD4+ T cells (p<0.01) than healthy controls. NTHi challenge significantly increased the proportion of activated (CD107a+) NK cells in otitis-prone and non-otitis-prone children (p<0.01), suggesting that NK cells from otitis-prone children are functional and respond to NTHi. CD8+ T cells and NK cells from both cases and controls produced IFNγ in response to polyclonal stimulus (Staphylococcal enterotoxin B; SEB), with more IFNγ+ CD8+ T cells present in cases than controls (p<0.05) but similar proportions of IFNγ+ NK cells. Otitis-prone children had more circulating IFNγ-producing NK cells (p<0.05) and more IFNγ-producing CD4+ (p<0.01) or CD8+ T-cells (p<0.05) than healthy controls. In response to SEB, more CD107a-expressing CD8+ T cells were present in cases than controls (p<0.01). Despite differences in PBMC composition, PBMC from otitis-prone children mounted innate and T cell-mediated responses to NTHi challenge that were comparable to healthy children. These data provide evidence that otitis-prone children do not have impaired functional cell mediated immunity.", "link"=>"http://www.mendeley.com/research/evidence-functional-cellmediated-immune-responses-nontypeable-haemophilus-influenzae-otitisprone-chi", "reader_count"=>3, "reader_count_by_academic_status"=>{"Librarian"=>1, "Researcher"=>1, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Librarian"=>1, "Researcher"=>1, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Social Sciences"=>1, "Unspecified"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Social Sciences"=>{"Social Sciences"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

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