Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells
Publication Date
April 04, 2018
Authors
Thomas Del’guidice, Jean Pascal Lepetit Stoffaes, Louis Jean Bordeleau, Joannie Roberge, et al
Volume
13
Issue
4
Pages
e0195558
DOI
https://dx.plos.org/10.1371/journal.pone.0195558
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0195558
Scopus
85045009280
Mendeley
http://www.mendeley.com/research/membrane-permeabilizing-amphiphilic-peptide-delivers-recombinant-transcription-factor-crisprcas9cpf1
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Mendeley | Further Information

{"title"=>"Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells", "type"=>"journal", "authors"=>[{"first_name"=>"Thomas", "last_name"=>"Del'Guidice", "scopus_author_id"=>"24075738000"}, {"first_name"=>"Jean Pascal", "last_name"=>"Lepetit-Stoffaes", "scopus_author_id"=>"57201470633"}, {"first_name"=>"Louis Jean", "last_name"=>"Bordeleau", "scopus_author_id"=>"57201472055"}, {"first_name"=>"Joannie", "last_name"=>"Roberge", "scopus_author_id"=>"57201470586"}, {"first_name"=>"Vanessa", "last_name"=>"Théberge", "scopus_author_id"=>"57201469840"}, {"first_name"=>"Coraline", "last_name"=>"Lauvaux", "scopus_author_id"=>"57194587830"}, {"first_name"=>"Xavier", "last_name"=>"Barbeau", "scopus_author_id"=>"57201473121"}, {"first_name"=>"Jessica", "last_name"=>"Trottier", "scopus_author_id"=>"57201471854"}, {"first_name"=>"Vibhuti", "last_name"=>"Dave", "scopus_author_id"=>"24802026100"}, {"first_name"=>"Denis Claude", "last_name"=>"Roy", "scopus_author_id"=>"57200575742"}, {"first_name"=>"Bruno", "last_name"=>"Gaillet", "scopus_author_id"=>"15922488900"}, {"first_name"=>"Alain", "last_name"=>"Garnier", "scopus_author_id"=>"7006525208"}, {"first_name"=>"David", "last_name"=>"Guay", "scopus_author_id"=>"36677195000"}], "year"=>2018, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"621545606", "doi"=>"10.1371/journal.pone.0195558", "issn"=>"19326203", "scopus"=>"2-s2.0-85045009280", "sgr"=>"85045009280", "isbn"=>"1111111111"}, "id"=>"038423a6-e29f-3556-9244-c18d744a9321", "abstract"=>"Delivery of recombinant proteins to therapeutic cells is limited by a lack of efficient methods. This hinders the use of transcription factors or Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) ribonucleoproteins to develop cell therapies. Here, we report a soluble peptide designed for the direct delivery of proteins to mammalian cells including human stem cells, hard-to-modify primary natural killer (NK) cells, and cancer cell models. This peptide is composed of a 6x histidine-rich domain fused to the endosomolytic peptide CM18 and the cell penetrating peptide PTD4. A less than two-minute co-incubation of 6His-CM18-PTD4 peptide with spCas9 and/or asCpf1 CRISPR ribonucleoproteins achieves robust gene editing. The same procedure, co-incubating with the transcription factor HoxB4, achieves transcriptional regulation. The broad applicability and flexibility of this DNA- and chemical-free method across different cell types, particularly hard-to-transfect cells, opens the way for a direct use of proteins for biomedical research and cell therapy manufacturing.", "link"=>"http://www.mendeley.com/research/membrane-permeabilizing-amphiphilic-peptide-delivers-recombinant-transcription-factor-crisprcas9cpf1", "reader_count"=>19, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Student > Bachelor"=>5, "Student > Doctoral Student"=>1, "Student > Postgraduate"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Student > Bachelor"=>5, "Student > Doctoral Student"=>1, "Student > Postgraduate"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>8, "Agricultural and Biological Sciences"=>7, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>8}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>0}

Scopus | Further Information

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