DNA microarray-based assessment of virulence potential of Shiga toxin gene-carrying Escherichia coli O104:H7 isolated from feedlot cattle feces
Publication Date
April 30, 2018
Authors
Pragathi B. Shridhar, Isha R. Patel, Jayanthi Gangiredla, Lance W. Noll, et al
Volume
13
Issue
4
Pages
e0196490
DOI
https://dx.plos.org/10.1371/journal.pone.0196490
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0196490
Scopus
85046295093
Mendeley
http://www.mendeley.com/research/dna-microarraybased-assessment-virulence-potential-shiga-toxin-gene-carrying-escherichia-coli-o104h7
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Mendeley | Further Information

{"title"=>"DNA microarray-based assessment of virulence potential of Shiga toxin gene- carrying Escherichia coli O104:H7 isolated from feedlot cattle feces", "type"=>"journal", "authors"=>[{"first_name"=>"Pragathi B", "last_name"=>"Shridhar"}, {"first_name"=>"Isha R", "last_name"=>"Patel"}, {"first_name"=>"Jayanthi", "last_name"=>"Gangiredla"}, {"first_name"=>"Lance W", "last_name"=>"Noll"}, {"first_name"=>"Xiaorong", "last_name"=>"Shi"}, {"first_name"=>"Jianfa", "last_name"=>"Bai"}, {"first_name"=>"Christopher A", "last_name"=>"Elkins"}, {"first_name"=>"Nancy", "last_name"=>"Strockbine"}, {"first_name"=>"T G", "last_name"=>"Nagaraja"}], "identifiers"=>{"sgr"=>"85046295093", "doi"=>"10.1371/journal.pone.0196490", "issn"=>"19326203", "scopus"=>"2-s2.0-85046295093", "pui"=>"621913318"}, "id"=>"10e26871-293f-3144-aba0-32d9f52573da", "abstract"=>"Escherichia coli O104:H4, a hybrid pathotype reported in a large 2011 foodborne outbreak in Germany, has not been detected in cattle feces. However, cattle harbor and shed in the feces other O104 serotypes, particularly O104:H7, which has been associated with sporadic cases of diarrhea in humans. The objective of our study was to assess the virulence poten-tial of Shiga toxin-producing E. coli (STEC) O104:H7 isolated from feces of feedlot cattle using DNA microarray. Six strains of STEC O104:H7 isolated from cattle feces were ana-lyzed using FDA-E. coli Identification (ECID) DNA microarray to determine their virulence profiles and compare them to the human strains (clinical) of O104:H7, STEC O104:H4 (Ger-man outbreak strain), and O104:H21 (milk-associated Montana outbreak strain). Scatter plots were generated from the array data to visualize the gene-level differences between bovine and human O104 strains, and Pearson correlation coefficients (r) were determined. Splits tree was generated to analyze relatedness between the strains. All O104:H7 strains, both bovine and human, similar to O104:H4 and O104:H21 outbreak strains were negative for intimin (eae). The bovine strains were positive for Shiga toxin 1 subtype c (stx1c), entero-hemolysin (ehxA), tellurite resistance gene (terD), IrgA homolog protein (iha), type 1 fim-briae (fimH), and negative for genes that code for effector proteins of type III secretory system. The six cattle O104 strains were closely related (r = 0.86–0.98) to each other, except for a few differences in phage related and non-annotated genes. One of the human clinical O104:H7 strains (2011C-3665) was more closely related to the bovine O104:H7 strains (r = 0.81–0.85) than the other four human clinical O104:H7 strains (r = 0.75–0.79). Montana outbreak strain (O104:H21) was more closely related to four of the human clinical O104:H7 strains than the bovine O104:H7 strains. None of the bovine E. coli O104 strains carried genes characteristic of E. coli O104:H4 German outbreak strain and unlike other human strains were also negative for Shiga toxin 2. Because cattle E. coli O104:H7 strains", "link"=>"http://www.mendeley.com/research/dna-microarraybased-assessment-virulence-potential-shiga-toxin-gene-carrying-escherichia-coli-o104h7", "reader_count"=>1, "reader_count_by_academic_status"=>{"Student > Ph. D. Student"=>1}, "reader_count_by_user_role"=>{"Student > Ph. D. Student"=>1}, "reader_count_by_subject_area"=>{"Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}}, "group_count"=>0}

Scopus | Further Information

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