The Glutathione Biosynthetic Pathway of Plasmodium Is Essential for Mosquito Transmission
Publication Date
February 20, 2009
Journal
PLOS Pathogens
Authors
Joel Vega Rodríguez, Blandine Franke Fayard, Rhoel R. Dinglasan, Chris J. Janse, et al
Volume
5
Issue
2
Pages
e1000302
DOI
https://dx.plos.org/10.1371/journal.ppat.1000302
Publisher URL
http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1000302
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/19229315
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2636896
Europe PMC
http://europepmc.org/abstract/MED/19229315
Web of Science
000263928000013
Scopus
61449205182
Mendeley
http://www.mendeley.com/research/glutathione-biosynthetic-pathway-plasmodium-essential-mosquito-transmission
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The glutathione biosynthetic pathway of Plasmodium is essential for mosquito transmission", "type"=>"journal", "authors"=>[{"first_name"=>"Joel", "last_name"=>"Vega-Rodríguez", "scopus_author_id"=>"26041091600"}, {"first_name"=>"Blandine", "last_name"=>"Franke-Fayard", "scopus_author_id"=>"6506436452"}, {"first_name"=>"Rhoel R.", "last_name"=>"Dinglasan", "scopus_author_id"=>"6507528785"}, {"first_name"=>"Chris J.", "last_name"=>"Janse", "scopus_author_id"=>"7005097427"}, {"first_name"=>"Rebecca", "last_name"=>"Pastrana-Mena", "scopus_author_id"=>"26041035900"}, {"first_name"=>"Andrew P.", "last_name"=>"Waters", "scopus_author_id"=>"7101758887"}, {"first_name"=>"Isabelle", "last_name"=>"Coppens", "scopus_author_id"=>"6604095394"}, {"first_name"=>"José F.", "last_name"=>"Rodríguez-Orengo", "scopus_author_id"=>"6505775907"}, {"first_name"=>"Marcelo", "last_name"=>"Jacobs-Lorena", "scopus_author_id"=>"7004974477"}, {"first_name"=>"Adelfa E.", "last_name"=>"Serrano", "scopus_author_id"=>"54390025900"}], "year"=>2009, "source"=>"PLoS Pathogens", "identifiers"=>{"pui"=>"354253507", "sgr"=>"61449205182", "pmid"=>"19229315", "scopus"=>"2-s2.0-61449205182", "isbn"=>"1553-7366", "doi"=>"10.1371/journal.ppat.1000302", "issn"=>"15537366"}, "id"=>"1054e6a5-a25a-37ce-b0f6-302989e99d02", "abstract"=>"Infection of red blood cells (RBC) subjects the malaria parasite to oxidative stress. Therefore, efficient antioxidant and redox systems are required to prevent damage by reactive oxygen species. Plasmodium spp. have thioredoxin and glutathione (GSH) systems that are thought to play a major role as antioxidants during blood stage infection. In this report, we analyzed a critical component of the GSH biosynthesis pathway using reverse genetics. Plasmodium berghei parasites lacking expression of gamma-glutamylcysteine synthetase (gamma-GCS), the rate limiting enzyme in de novo synthesis of GSH, were generated through targeted gene disruption thus demonstrating, quite unexpectedly, that gamma-GCS is not essential for blood stage development. Despite a significant reduction in GSH levels, blood stage forms of pbggcs(-) parasites showed only a defect in growth as compared to wild type. In contrast, a dramatic effect on development of the parasites in the mosquito was observed. Infection of mosquitoes with pbggcs(-) parasites resulted in reduced numbers of stunted oocysts that did not produce sporozoites. These results have important implications for the design of drugs aiming at interfering with the GSH redox-system in blood stages and demonstrate that de novo synthesis of GSH is pivotal for development of Plasmodium in the mosquito.", "link"=>"http://www.mendeley.com/research/glutathione-biosynthetic-pathway-plasmodium-essential-mosquito-transmission", "reader_count"=>69, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>18, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>24, "Student > Postgraduate"=>2, "Other"=>1, "Student > Master"=>8, "Student > Bachelor"=>5, "Lecturer"=>2, "Professor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>18, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>24, "Student > Postgraduate"=>2, "Other"=>1, "Student > Master"=>8, "Student > Bachelor"=>5, "Lecturer"=>2, "Professor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>12, "Agricultural and Biological Sciences"=>41, "Medicine and Dentistry"=>4, "Chemistry"=>6, "Computer Science"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>6}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>41}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Puerto Rico"=>1, "United States"=>3, "United Kingdom"=>1, "Portugal"=>1, "Germany"=>1}, "group_count"=>1}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/61449205182"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/61449205182?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=61449205182&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=61449205182&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/61449205182", "dc:identifier"=>"SCOPUS_ID:61449205182", "eid"=>"2-s2.0-61449205182", "dc:title"=>"The glutathione biosynthetic pathway of Plasmodium is essential for mosquito transmission", "dc:creator"=>"Vega-Rodríguez J.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"5", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2009-02-01", "prism:coverDisplayDate"=>"February 2009", "prism:doi"=>"10.1371/journal.ppat.1000302", "citedby-count"=>"47", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Puerto Rico School of Medicine", "affiliation-city"=>"San Juan", "affiliation-country"=>"Puerto Rico"}], "pubmed-id"=>"19229315", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1000302", "source-id"=>"4000151809", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplospathogens%2Farticle%3Fid%3D10.1371%252Fjournal.ppat.1000302", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"2", "year"=>"2009", "pdf_views"=>"136", "xml_views"=>"3", "html_views"=>"382"}
  • {"month"=>"3", "year"=>"2009", "pdf_views"=>"111", "xml_views"=>"0", "html_views"=>"285"}
  • {"month"=>"4", "year"=>"2009", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"5", "year"=>"2009", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"6", "year"=>"2009", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"7", "year"=>"2009", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"66"}
  • {"month"=>"8", "year"=>"2009", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"9", "year"=>"2009", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"10", "year"=>"2009", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2009", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"12", "year"=>"2009", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"1", "year"=>"2010", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"2", "year"=>"2010", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"3", "year"=>"2010", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"4", "year"=>"2010", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"57"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"67"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"89"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"119"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"5", "html_views"=>"247"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"225"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"98"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"75"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"123"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"112"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"94"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"241"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"192"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"151"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"118"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"108"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"4", "html_views"=>"104"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"65"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"64"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"34"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"38"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"4", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"7", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"5", "html_views"=>"7"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"7", "html_views"=>"10"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/908014"], "description"=>"<p>(A) Oocysts on dissected midguts at 2 d.p.i., reacted with antibody Mab 13.1 against the ookinete/young oocysts surface protein Pbs21. Oocysts were detected after incubation with a Rhodamine Red™-X goat anti-mouse IgG (H+L) secondary antibody (Invitrogen). No differences in the reactivity pattern of oocysts from <i>pbgccs</i><sup>−</sup> and wild type parasites were observed. (B) Oocysts on dissected midguts at day 12 p.i. stained with 0.2% mercurochrome (upper panel), antibodies against PbCap380 in the oocyst capsule (center panel) and antibodies against the surface protein of sporozoites PbCS (Mab 3D11). Antibody-stained oocysts were detected by indirect fluorescence microscopy using Rhodamine Red™-X goat anti-mouse IgG (H+L) or Texas Red®-X goat anti-rabbit IgG (H+L) secondary antibodies (Invitrogen), respectively. The <i>pbggcs<sup>−</sup></i> oocysts produced the PbCap380 protein but are strongly reduced in size compared to wild type (see also C). Anti-CS staining shows that, although CS is produced, 12 day old oocysts lack the typical CS staining pattern of wild type oocysts. (C) Oocyst size on dissected midguts 12 d.p.i.</p>", "links"=>[], "tags"=>["oocysts"], "article_id"=>578464, "categories"=>["Molecular Biology", "Microbiology", "Biochemistry", "Physics"], "users"=>["Joel Vega-Rodríguez", "Blandine Franke-Fayard", "Rhoel R. Dinglasan", "Chris J. Janse", "Rebecca Pastrana-Mena", "Andrew P. Waters", "Isabelle Coppens", "José F. Rodríguez-Orengo", "Marcelo Jacobs-Lorena", "Adelfa E. Serrano"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000302.g005", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Development_of_pbggcs_8722_oocysts_in_A_stephensi_mosquitoes_/578464", "title"=>"Development of <i>pbggcs<sup>−</sup></i> oocysts in <i>A. stephensi</i> mosquitoes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 06:50:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/908470"], "description"=>"<p>(A) Diagrammatic representation of the construct (<i>pbggcs-comp</i>) used <i>for</i> complementation (top), the <i>dssurrna</i> genomic locus used for targeted integration of the construct (center), and the locus after integration of the construct by single cross-over recombination (bottom). The <i>pbggcs-comp</i> vector contains the <i>hdhfr</i> selectable marker and the <i>dssurrna</i> targeting sequence. Dashed lines represent the probes used for Southern analysis (see B). (B) Southern analysis of CHEF separated chromosomes from <i>pbggcs<sup>−</sup>, pbggcs-comp,</i> and <i>pbggcs-comp*</i> parasites (asterisk indicates complemented parasites after mosquito passage). Chromosomes were hybridized to different probes showing correct integration of the <i>pbggcs-comp</i> plasmid in chromosome 5, which contains the target <i>dssurrna</i> locus. (C) Reverse transcriptase PCR analysis showing the presence of <i>pbggcs</i> transcripts (345 bp fragments) in wild type and in <i>pbggcs-comp</i> parasites and the absence of <i>pbggcs</i> transcripts in <i>pbggcs<sup>−</sup></i> parasites. For the PCR reaction, the specific <i>pbggcs</i> primers PIY-FM/DamR were used (see A). cDNA was synthesized from total mRNA and control reactions described in <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000302#ppat-1000302-g001\" target=\"_blank\">Fig. 1C</a>. (D) Presence of maturing oocysts (stained with mercurochrome) in midguts from mosquitoes infected with <i>pbggcs-comp</i> parasites. Note the internal structure developing inside the oocysts, indicative of segmentation during sporogony and formation of sporozoites.</p>", "links"=>[], "tags"=>["complementation", "parasites"], "article_id"=>578921, "categories"=>["Molecular Biology", "Microbiology", "Biochemistry", "Physics"], "users"=>["Joel Vega-Rodríguez", "Blandine Franke-Fayard", "Rhoel R. Dinglasan", "Chris J. Janse", "Rebecca Pastrana-Mena", "Andrew P. Waters", "Isabelle Coppens", "José F. Rodríguez-Orengo", "Marcelo Jacobs-Lorena", "Adelfa E. Serrano"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000302.g008", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genetic_complementation_of_the_pbggcs_8722_parasites_with_the_wild_type_pbgccs_gene_/578921", "title"=>"Genetic complementation of the <i>pbggcs<sup>−</sup></i> parasites with the wild type <i>pbgccs</i> gene.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 06:53:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/907727"], "description"=>"<p>The course of parasitemia was determined in groups of 5 mice infected with either 2×10<sup>2</sup> (A) or 2×10<sup>3</sup> (B) parasites and followed for 10 days. Each point represents the mean parasitemia. Bars represent standard errors of the mean.</p>", "links"=>[], "tags"=>["parasites"], "article_id"=>578178, "categories"=>["Molecular Biology", "Microbiology", "Biochemistry", "Physics"], "users"=>["Joel Vega-Rodríguez", "Blandine Franke-Fayard", "Rhoel R. Dinglasan", "Chris J. Janse", "Rebecca Pastrana-Mena", "Andrew P. Waters", "Isabelle Coppens", "José F. Rodríguez-Orengo", "Marcelo Jacobs-Lorena", "Adelfa E. Serrano"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000302.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proliferation_of_wild_type_and_pbgccs_8722_parasites_in_mice_/578178", "title"=>"Proliferation of wild type and <i>pbgccs<sup>−</sup></i> parasites in mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 06:48:49"}
  • {"files"=>["https://ndownloader.figshare.com/files/907536"], "description"=>"<p>(A) Diagrammatic representation of the <i>pbggcs</i> replacement vectors (top), the <i>pbggcs</i> genomic locus (center), and the predicted integration event (bottom). The <i>pbggcs-ko</i> vectors contained the <i>tgdhfr/ts</i> selectable cassette flanked by 5′ and 3′ fragments of the <i>pbggcs</i> gene. Dashed lines inside the coding regions of <i>pbggcs</i> and <i>tgdhfr/ts</i> represent the probes used in Southern blot analysis (see B). Arrows represent primers used for PCR analysis of expression (see C). (B) Southern analysis of SpeI/XhoI digested gDNA from wild type and <i>pbggcs<sup>−</sup></i> parasites. The two expected DNA fragments of 4.5 kb and 7.0 kb were obtained after hybridization with a <i>tgdhfr</i>/<i>ts</i> specific probe (left panel) demonstrating correct integration of the <i>pbggcs-ko</i> vector. Hybridization with the <i>pbggcs</i> specific probe shows the absence in the <i>pbggcs<sup>−</sup></i> parasites of the 4.5 kb fragment that corresponds to wild type <i>pbggcs</i>. pL1217: <i>pbggcs</i> disruption construct. (C) Reverse transcriptase PCR analysis (RT-PCR) showing absence of <i>pbggcs</i> transcripts in <i>pbggcs<sup>−</sup></i> parasites. For each cDNA synthesis reaction (C) controls included: no reverse transcriptase (N), gDNA (G), and no DNA (−). Hep17 primers were included in the RT-PCR reaction to rule out the presence of gDNA in the cDNA samples. (D) Northern analysis of mRNA of wild type and <i>pbggcs<sup>−</sup></i> parasites. Hybridization with a <i>pbggcs</i>-specific probe shows the absence of <i>pbggcs</i> transcripts in <i>pbggcs<sup>−</sup></i> parasites. As a loading control the Northern blot was hybridized with a ribosomal RNA probe (primer L647).</p>", "links"=>[], "tags"=>["Biochemistry", "infectious diseases/protozoal infections", "microbiology/cellular microbiology and pathogenesis", "microbiology/parasitology", "molecular biology"], "article_id"=>577968, "categories"=>["Molecular Biology", "Microbiology", "Biochemistry", "Physics"], "users"=>["Joel Vega-Rodríguez", "Blandine Franke-Fayard", "Rhoel R. Dinglasan", "Chris J. Janse", "Rebecca Pastrana-Mena", "Andrew P. Waters", "Isabelle Coppens", "José F. Rodríguez-Orengo", "Marcelo Jacobs-Lorena", "Adelfa E. Serrano"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000302.g001", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Disruption_of_the_pbggcs_gene_/577968", "title"=>"Disruption of the <i>pbggcs</i> gene.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 06:47:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/907865"], "description"=>"<p>(A) Ookinete numbers were determined by light microscopy analysis of midguts dissected at 24 h.p.i. Horizontal lines indicate the median number of ookinetes for each group. Statistical significance was determined by Mann Whitney U Test, α = 0.05. (B) Oocyst numbers were determined on dissected midguts at 2 and 12 d.p.i. At day 2 p.i., oocysts were counted with the aid of a fluorescence microscope after incubation of midguts with an antibody against the ookinete/young oocyst surface protein Pbs21. At day 12 p.i., oocysts were stained with mercurochrome and counted using a light microscope. Horizontal lines indicate median oocyst number for each group. Statistical significance was determined by Mann Whitney U Test, α = 0.05. (C) Sporozoite infection of salivary glands was determined by dissection at day 18 p.i. and analysis of GFP-expressing sporozoites using a fluorescence microscope. Results are shown as the number of salivary glands infected with sporozoites. (N) Sample size; Prevalence: Percentage of infected mosquitoes/tissues in each group; Percent inhibition: [((control median parasite number – transfectant median parasite number)/control median parasite number)×100].</p>", "links"=>[], "tags"=>["sporozoites"], "article_id"=>578315, "categories"=>["Molecular Biology", "Microbiology", "Biochemistry", "Physics"], "users"=>["Joel Vega-Rodríguez", "Blandine Franke-Fayard", "Rhoel R. Dinglasan", "Chris J. Janse", "Rebecca Pastrana-Mena", "Andrew P. Waters", "Isabelle Coppens", "José F. Rodríguez-Orengo", "Marcelo Jacobs-Lorena", "Adelfa E. Serrano"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000302.g004", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Development_of_pbggcs_8722_ookinetes_oocysts_and_sporozoites_in_A_stephensi_mosquitoes_/578315", "title"=>"Development of <i>pbggcs<sup>−</sup></i> ookinetes, oocysts, and sporozoites in <i>A. stephensi</i> mosquitoes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 06:49:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/908310"], "description"=>"<p>Electron micrographs of wild-type (A, B, D, E) and <i>pbggcs<sup>−</sup></i> (C–C') oocysts at day 12 p.i. (A) Encapsulated oocyst (c, capsule) at mid-sporogony showing numerous irregular nuclear profiles, endoplasmic reticulum (ER), and mitochondria (m) localized between the nuclei (n). (B) Mature oocysts containing sporozoites. The newly formed sporozoites show the trilaminar pellicle, including the inner membrane complex (IMC), subpellicular microtubules (Mt), nucleus, and secretory organelles such as rhoptries (r). (C, C') Two serial sections of the same <i>pbggcs<sup>−</sup></i> oocyst revealing the absence of internal structures characteristics of parasites undergoing sporogony (see A). The extracellular capsule is present but shows a more irregular thickness compared to wild type oocysts (see D). The nucleus is pycnotic and ER elements are largely vacuolized, compared to the branched and spiraled network of ER in the wild type oocyst (see E). (D) Morphology of capsule of wild-type oocysts. (E) ER of wild type oocysts.</p>", "links"=>[], "tags"=>["morphology", "electron"], "article_id"=>578762, "categories"=>["Molecular Biology", "Microbiology", "Biochemistry", "Physics"], "users"=>["Joel Vega-Rodríguez", "Blandine Franke-Fayard", "Rhoel R. Dinglasan", "Chris J. Janse", "Rebecca Pastrana-Mena", "Andrew P. Waters", "Isabelle Coppens", "José F. Rodríguez-Orengo", "Marcelo Jacobs-Lorena", "Adelfa E. Serrano"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000302.g007", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Oocysts_of_pbggcs_8722_parasites_analysis_of_morphology_by_transmission_electron_microscopy_/578762", "title"=>"Oocysts of <i>pbggcs<sup>−</sup></i> parasites: analysis of morphology by transmission electron microscopy.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 06:52:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/907655"], "description"=>"<p>Intracellular GSH levels were determined by HPLC with a fluorescence detector as described in <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000302#s4\" target=\"_blank\">Materials and Methods</a>. (A) Typical chromatogram of <i>P. berghei</i> parasite extracts showing the signal for total GSH (retention time 5.34 min). (B) Representative calibration curve using GSH standards in a range between 4 and 24 µM. (C) Comparison of total GSH concentration of WT, <i>pbggcs<sup>−</sup></i>1, <i>pbggcs<sup>−</sup></i>2, and <i>pbggcs-comp</i> parasites. GSH levels were determined in extracts of purified blood stages obtained from mice with asynchronous infections. A One Way ANOVA test shows statistically significant differences in GSH concentration between <i>pbggcs<sup>−</sup></i> parasites and wild type (P<0.0001) and between <i>pbggcs<sup>−</sup></i> and <i>pbggcs-comp</i> parasites (P<0.0001). Numbers on boxes represent the mean concentration of GSH. Bars represent the standard error of the mean. Wild type n = 20, <i>pbggcs<sup>−</sup></i>1 n = 16, <i>pbggcs<sup>−</sup></i>2 n = 22, <i>pbggcs-comp</i> n = 16.</p>", "links"=>[], "tags"=>["glutathione", "levels", "stages"], "article_id"=>578103, "categories"=>["Molecular Biology", "Microbiology", "Biochemistry", "Physics"], "users"=>["Joel Vega-Rodríguez", "Blandine Franke-Fayard", "Rhoel R. Dinglasan", "Chris J. Janse", "Rebecca Pastrana-Mena", "Andrew P. Waters", "Isabelle Coppens", "José F. Rodríguez-Orengo", "Marcelo Jacobs-Lorena", "Adelfa E. Serrano"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000302.g002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Total_glutathione_GSH_levels_in_blood_stages_of_wild_type_pbgccs_8722_and_pbggcs_comp_parasites_/578103", "title"=>"Total glutathione (GSH) levels in blood stages of wild type, <i>pbgccs<sup>−</sup></i>, and <i>pbggcs-comp</i> parasites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 06:48:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/908164"], "description"=>"<p>Midguts from <i>A. stephensi</i> infected with either wild type or <i>pbggcs<sup>−</sup></i> parasites were dissected at day 12 p.i. and stained with MitoTracker Red (Molecular Probes). (A) Midgut containing wild type oocysts expressing GFP. Note that in this merge image of GFP and MitoTracker fluorescence, GFP fluorescence was completely quenched by the MitoTracker fluorescence. (B) Uninfected midgut (MG). Note the distinctive staining pattern of mitochondria in the gut cell surrounding the nucleus. (C, D) Midguts containing oocysts from <i>pbggcs<sup>−</sup></i> 1 and <i>pbggcs<sup>−</sup></i> 2 parasites, respectively. Note in this merged images of GFP and MitoTracker fluorescence, some oocysts show a partial staining with MitoTracker (arrows) while others do not stain. All panels are merged except for panel B.</p>", "links"=>[], "tags"=>["mitochondria", "oocysts", "mitotracker"], "article_id"=>578614, "categories"=>["Molecular Biology", "Microbiology", "Biochemistry", "Physics"], "users"=>["Joel Vega-Rodríguez", "Blandine Franke-Fayard", "Rhoel R. Dinglasan", "Chris J. Janse", "Rebecca Pastrana-Mena", "Andrew P. Waters", "Isabelle Coppens", "José F. Rodríguez-Orengo", "Marcelo Jacobs-Lorena", "Adelfa E. Serrano"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000302.g006", "stats"=>{"downloads"=>0, "page_views"=>30, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Labeling_of_mitochondria_in_live_pbgccs_8722_oocysts_using_MitoTracker_Red_/578614", "title"=>"Labeling of mitochondria in live <i>pbgccs<sup>−</sup></i> oocysts using MitoTracker Red.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 06:51:22"}

PMC Usage Stats | Further Information

  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"27", "year"=>"2010", "pdf"=>"11", "unique-ip"=>"23", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"19", "unique-ip"=>"23", "pdf"=>"6", "year"=>"2010", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"22", "year"=>"2010", "pdf"=>"10", "unique-ip"=>"26", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"26", "unique-ip"=>"20", "pdf"=>"16", "year"=>"2010", "figure"=>"15", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"23", "year"=>"2010", "pdf"=>"16", "unique-ip"=>"24", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"1", "abstract"=>"3", "full-text"=>"11", "unique-ip"=>"15", "pdf"=>"3", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "year"=>"2010", "pdf"=>"9", "unique-ip"=>"19", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"6", "full-text"=>"14", "unique-ip"=>"23", "pdf"=>"16", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"18", "year"=>"2010", "pdf"=>"12", "unique-ip"=>"25", "figure"=>"15", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"15", "unique-ip"=>"17", "pdf"=>"11", "year"=>"2010", "figure"=>"9", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "year"=>"2010", "pdf"=>"6", "unique-ip"=>"11", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"16", "unique-ip"=>"16", "pdf"=>"6", "year"=>"2010", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"18", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"15", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"10", "unique-ip"=>"13", "pdf"=>"5", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"19", "year"=>"2011", "pdf"=>"7", "unique-ip"=>"16", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"10", "pdf"=>"4", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"9", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"12", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"17", "unique-ip"=>"18", "pdf"=>"9", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"15", "year"=>"2011", "pdf"=>"6", "unique-ip"=>"11", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"10", "unique-ip"=>"10", "pdf"=>"4", "year"=>"2011", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"10", "year"=>"2011", "pdf"=>"5", "unique-ip"=>"11", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"12", "pdf"=>"5", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "year"=>"2011", "pdf"=>"5", "unique-ip"=>"7", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"5", "unique-ip"=>"5", "pdf"=>"4", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"1", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "year"=>"2012", "pdf"=>"0", "unique-ip"=>"6", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "unique-ip"=>"9", "pdf"=>"4", "year"=>"2012", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "year"=>"2012", "pdf"=>"5", "unique-ip"=>"12", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "unique-ip"=>"5", "pdf"=>"3", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"4", "year"=>"2012", "pdf"=>"3", "unique-ip"=>"4", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"2", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"36", "unique-ip"=>"37", "pdf"=>"19", "year"=>"2009", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"3", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"78", "year"=>"2009", "pdf"=>"57", "unique-ip"=>"69", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"4", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"35", "unique-ip"=>"32", "pdf"=>"18", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"5", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"35", "year"=>"2009", "pdf"=>"30", "unique-ip"=>"36", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"6", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"28", "unique-ip"=>"28", "pdf"=>"13", "year"=>"2009", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"7", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"33", "year"=>"2009", "pdf"=>"16", "unique-ip"=>"32", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"8", "cited-by"=>"0", "abstract"=>"4", "full-text"=>"21", "unique-ip"=>"22", "pdf"=>"11", "year"=>"2009", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"9", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"32", "year"=>"2009", "pdf"=>"15", "unique-ip"=>"31", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"10", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"11", "unique-ip"=>"12", "pdf"=>"9", "year"=>"2009", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"6", "full-text"=>"51", "year"=>"2009", "pdf"=>"13", "unique-ip"=>"33", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"22", "unique-ip"=>"23", "pdf"=>"6", "year"=>"2009", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"4", "year"=>"2012", "pdf"=>"9", "unique-ip"=>"11", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"23", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"23", "full-text"=>"28", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"28", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"25", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"21", "full-text"=>"23", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"22", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"21", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"24", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"33", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"23", "full-text"=>"32", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"19", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"21", "full-text"=>"19", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"20", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}

Relative Metric

{"start_date"=>"2009-01-01T00:00:00Z", "end_date"=>"2009-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[349, 614, 744, 851, 955, 1059, 1167, 1262, 1352, 1431, 1516, 1593, 1668, 1746, 1817, 1883, 1950, 2017, 2075, 2141, 2198, 2254, 2313, 2368, 2424, 2474, 2534, 2591, 2651, 2710, 2776, 2837, 2892, 2953, 3014, 3072, 3130, 3186, 3251, 3307, 3366, 3427, 3500, 3561, 3627, 3688, 3759, 3821, 3888, 3950, 4010, 4070, 4131, 4189, 4242, 4307, 4370, 4431, 4491, 4549, 4608]}, {"subject_area"=>"/Biology and life sciences/Anatomy and physiology", "average_usage"=>[320, 564, 690, 796, 901, 1003, 1105, 1196, 1286, 1367, 1453, 1523, 1601, 1673, 1743, 1807, 1879, 1931, 1993, 2053, 2108, 2165, 2218, 2273, 2327, 2375, 2432, 2492, 2539, 2590, 2639, 2694, 2742, 2807, 2861, 2918, 2977, 3032, 3086, 3145, 3204, 3260, 3326, 3378, 3438, 3496, 3558, 3626]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[334, 613, 751, 863, 964, 1078, 1181, 1270, 1358, 1443, 1536, 1616, 1690, 1768, 1826, 1902, 1969, 2031, 2095, 2149, 2202, 2258, 2305, 2349, 2417, 2482, 2541, 2598, 2662, 2720, 2768, 2832, 2897, 2947, 2998, 3058, 3121, 3175, 3235, 3292, 3368, 3442, 3507, 3563, 3621, 3676, 3736, 3802, 3852, 3922, 3977, 4025, 4071, 4141, 4199, 4258, 4316, 4376, 4422, 4467, 4518]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[337, 605, 735, 844, 939, 1037, 1139, 1214, 1300, 1375, 1461, 1543, 1619, 1693, 1768, 1827, 1881, 1948, 2013, 2066, 2118, 2172, 2227, 2270, 2325, 2386, 2443, 2504, 2565, 2609, 2659, 2731, 2792, 2857, 2920, 2984, 3051, 3101, 3165, 3213, 3276, 3344, 3397, 3462, 3512, 3561, 3629, 3710, 3779, 3847, 3897, 3949, 4013, 4086, 4140, 4180, 4239, 4288, 4344, 4409, 4470]}, {"subject_area"=>"/Biology and life sciences/Parasitology", "average_usage"=>[333, 557, 666, 769, 870, 981, 1073, 1139, 1199, 1269, 1375, 1438, 1505, 1553, 1632, 1680, 1736, 1808, 1874, 1941, 1996, 2052, 2096, 2145, 2173, 2204, 2257, 2304, 2346, 2426, 2472, 2508, 2551, 2615, 2668, 2696, 2761, 2814, 2861, 2928, 3033, 3082, 3181, 3261, 3320, 3367, 3445, 3502, 3610, 3671, 3724, 3766, 3819, 3858, 3936, 4038, 4100, 4152, 4198, 4244, 4305, 4363]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[332, 584, 719, 824, 927, 1030, 1139, 1233, 1322, 1402, 1483, 1570, 1649, 1730, 1806, 1873, 1939, 2003, 2068, 2130, 2191, 2248, 2302, 2362, 2427, 2485, 2544, 2599, 2653, 2709, 2778, 2840, 2896, 2950, 3013, 3073, 3137, 3189, 3253, 3305, 3364, 3423, 3489, 3557, 3606, 3668, 3742, 3797, 3852, 3907, 3962, 4027, 4072, 4132, 4193, 4256, 4308, 4366, 4427, 4493, 4556]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[339, 580, 713, 827, 926, 1032, 1153, 1258, 1348, 1429, 1522, 1603, 1687, 1772, 1834, 1905, 1972, 2029, 2098, 2151, 2214, 2275, 2335, 2388, 2446, 2496, 2553, 2604, 2653, 2705, 2780, 2822, 2890, 2947, 3020, 3070, 3114, 3162, 3226, 3285, 3353, 3433, 3500, 3547, 3592, 3639, 3700, 3769, 3858, 3922, 3967, 4028, 4069, 4137, 4191, 4246, 4302, 4372, 4436, 4498, 4579]}, {"subject_area"=>"/Medicine and health sciences/Anatomy and physiology", "average_usage"=>[319, 558, 683, 792, 899, 999, 1104, 1192, 1280, 1362, 1441, 1525, 1601, 1673, 1751, 1819, 1897, 1955, 2015, 2078, 2122, 2184, 2233, 2297, 2342, 2393, 2449, 2504, 2557, 2614, 2655, 2715, 2789, 2834, 2890, 2950, 3013, 3063, 3123, 3194, 3242, 3303, 3365, 3424, 3480, 3546, 3612, 3682]}, {"subject_area"=>"/Medicine and health sciences/Parasitic diseases", "average_usage"=>[253, 507, 616, 727, 835, 909, 985, 1060, 1132, 1194, 1253, 1340, 1410, 1474, 1548, 1610, 1675, 1733, 1776, 1841, 1911, 1981, 2012, 2061, 2114, 2171, 2207, 2266, 2318, 2380, 2425, 2479, 2539, 2586, 2667, 2736, 2791, 2835, 2896, 2955, 3027, 3088, 3165, 3242, 3310, 3364, 3437, 3497, 3564, 3652, 3708, 3761, 3809, 3866, 3960, 4061, 4113, 4168, 4258, 4325, 4383, 4487]}]}
Loading … Spinner
There are currently no alerts