Dynamics of HIV-1 Assembly and Release
Publication Date
November 06, 2009
Journal
PLOS Pathogens
Authors
Sergey Ivanchenko, William J. Godinez, Marko Lampe, Hans Georg Kräusslich, et al
Volume
5
Issue
11
Pages
e1000652
DOI
https://dx.plos.org/10.1371/journal.ppat.1000652
Publisher URL
http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1000652
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/19893629
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766258
Europe PMC
http://europepmc.org/abstract/MED/19893629
Web of Science
000274226600007
Scopus
73549091724
Mendeley
http://www.mendeley.com/research/dynamics-hiv1-assembly-release
Events
Loading … Spinner

CiteULike | Further Information

Mendeley | Further Information

{"title"=>"Dynamics of HIV-1 assembly and release", "type"=>"journal", "authors"=>[{"first_name"=>"Sergey", "last_name"=>"Ivanchenko", "scopus_author_id"=>"6602149599"}, {"first_name"=>"William J.", "last_name"=>"Godinez", "scopus_author_id"=>"15042103700"}, {"first_name"=>"Marko", "last_name"=>"Lampe", "scopus_author_id"=>"23008808300"}, {"first_name"=>"Hans Georg", "last_name"=>"Kräusslich", "scopus_author_id"=>"7006112877"}, {"first_name"=>"Roland", "last_name"=>"Eils", "scopus_author_id"=>"7003976942"}, {"first_name"=>"Karl", "last_name"=>"Rohr", "scopus_author_id"=>"55332030200"}, {"first_name"=>"Christoph", "last_name"=>"Bräuchle", "scopus_author_id"=>"7005564729"}, {"first_name"=>"Barbara", "last_name"=>"Müller", "scopus_author_id"=>"7403187010"}, {"first_name"=>"Don C.", "last_name"=>"Lamb", "scopus_author_id"=>"7202727460"}], "year"=>2009, "source"=>"PLoS Pathogens", "identifiers"=>{"scopus"=>"2-s2.0-73549091724", "sgr"=>"73549091724", "issn"=>"15537366", "isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "pmid"=>"19893629", "doi"=>"10.1371/journal.ppat.1000652", "pui"=>"358060462"}, "id"=>"d0a66f51-777c-3072-bc1f-3bf51dee1aa0", "abstract"=>"Assembly and release of human immunodeficiency virus (HIV) occur at the plasma membrane of infected cells and are driven by the Gag polyprotein. Previous studies analyzed viral morphogenesis using biochemical methods and static images, while dynamic and kinetic information has been lacking until very recently. Using a combination of wide-field and total internal reflection fluorescence microscopy, we have investigated the assembly and release of fluorescently labeled HIV-1 at the plasma membrane of living cells with high time resolution. Gag assembled into discrete clusters corresponding to single virions. Formation of multiple particles from the same site was rarely observed. Using a photoconvertible fluorescent protein fused to Gag, we determined that assembly was nucleated preferentially by Gag molecules that had recently attached to the plasma membrane or arrived directly from the cytosol. Both membrane-bound and cytosol derived Gag polyproteins contributed to the growing bud. After their initial appearance, assembly sites accumulated at the plasma membrane of individual cells over 1-2 hours. Assembly kinetics were rapid: the number of Gag molecules at a budding site increased, following a saturating exponential with a rate constant of approximately 5 x 10(-3) s(-1), corresponding to 8-9 min for 90% completion of assembly for a single virion. Release of extracellular particles was observed at approximately 1,500+/-700 s after the onset of assembly. The ability of the virus to recruit components of the cellular ESCRT machinery or to undergo proteolytic maturation, or the absence of Vpu did not significantly alter the assembly kinetics.", "link"=>"http://www.mendeley.com/research/dynamics-hiv1-assembly-release", "reader_count"=>156, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>9, "Researcher"=>36, "Student > Doctoral Student"=>11, "Student > Ph. D. Student"=>58, "Student > Postgraduate"=>5, "Student > Master"=>13, "Other"=>4, "Student > Bachelor"=>7, "Professor"=>10}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>9, "Researcher"=>36, "Student > Doctoral Student"=>11, "Student > Ph. D. Student"=>58, "Student > Postgraduate"=>5, "Student > Master"=>13, "Other"=>4, "Student > Bachelor"=>7, "Professor"=>10}, "reader_count_by_subject_area"=>{"Unspecified"=>6, "Agricultural and Biological Sciences"=>92, "Chemical Engineering"=>1, "Chemistry"=>7, "Computer Science"=>1, "Engineering"=>6, "Biochemistry, Genetics and Molecular Biology"=>17, "Mathematics"=>1, "Medicine and Dentistry"=>7, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Physics and Astronomy"=>9, "Social Sciences"=>1, "Immunology and Microbiology"=>6}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>7}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>9}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>6}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Engineering"=>{"Engineering"=>6}, "Chemistry"=>{"Chemistry"=>7}, "Neuroscience"=>{"Neuroscience"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>6}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>92}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>17}}, "reader_count_by_country"=>{"Netherlands"=>1, "United States"=>9, "China"=>1, "United Kingdom"=>3, "Italy"=>1, "South Africa"=>1, "Switzerland"=>2, "Portugal"=>1, "Germany"=>1}, "group_count"=>3}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/73549091724"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/73549091724?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=73549091724&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=73549091724&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/73549091724", "dc:identifier"=>"SCOPUS_ID:73549091724", "eid"=>"2-s2.0-73549091724", "dc:title"=>"Dynamics of HIV-1 assembly and release", "dc:creator"=>"Ivanchenko S.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"5", "prism:issueIdentifier"=>"11", "prism:pageRange"=>nil, "prism:coverDate"=>"2009-11-01", "prism:coverDisplayDate"=>"November 2009", "prism:doi"=>"10.1371/journal.ppat.1000652", "citedby-count"=>"129", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Ludwig-Maximilians-Universität München", "affiliation-city"=>"Munich", "affiliation-country"=>"Germany"}], "pubmed-id"=>"19893629", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1000652", "source-id"=>"4000151809", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplospathogens%2Farticle%3Fid%3D10.1371%252Fjournal.ppat.1000652", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"11", "year"=>"2009", "pdf_views"=>"308", "xml_views"=>"6", "html_views"=>"912"}
  • {"month"=>"12", "year"=>"2009", "pdf_views"=>"108", "xml_views"=>"2", "html_views"=>"326"}
  • {"month"=>"1", "year"=>"2010", "pdf_views"=>"71", "xml_views"=>"3", "html_views"=>"236"}
  • {"month"=>"2", "year"=>"2010", "pdf_views"=>"51", "xml_views"=>"0", "html_views"=>"176"}
  • {"month"=>"3", "year"=>"2010", "pdf_views"=>"77", "xml_views"=>"0", "html_views"=>"218"}
  • {"month"=>"4", "year"=>"2010", "pdf_views"=>"51", "xml_views"=>"3", "html_views"=>"132"}
  • {"month"=>"5", "year"=>"2010", "pdf_views"=>"43", "xml_views"=>"0", "html_views"=>"203"}
  • {"month"=>"6", "year"=>"2010", "pdf_views"=>"46", "xml_views"=>"0", "html_views"=>"151"}
  • {"month"=>"7", "year"=>"2010", "pdf_views"=>"42", "xml_views"=>"0", "html_views"=>"133"}
  • {"month"=>"8", "year"=>"2010", "pdf_views"=>"35", "xml_views"=>"0", "html_views"=>"94"}
  • {"month"=>"9", "year"=>"2010", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"91"}
  • {"month"=>"10", "year"=>"2010", "pdf_views"=>"39", "xml_views"=>"1", "html_views"=>"117"}
  • {"month"=>"11", "year"=>"2010", "pdf_views"=>"34", "xml_views"=>"1", "html_views"=>"100"}
  • {"month"=>"12", "year"=>"2010", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"1", "year"=>"2011", "pdf_views"=>"23", "xml_views"=>"2", "html_views"=>"71"}
  • {"month"=>"2", "year"=>"2011", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"3", "year"=>"2011", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"4", "year"=>"2011", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"27", "xml_views"=>"1", "html_views"=>"75"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"73"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"104"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"34", "xml_views"=>"0", "html_views"=>"114"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"27", "xml_views"=>"2", "html_views"=>"103"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"37", "xml_views"=>"1", "html_views"=>"119"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"5", "html_views"=>"50"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"85"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"123"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"131"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"137"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"22", "xml_views"=>"2", "html_views"=>"289"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"107"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"128"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"122"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"104"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"87"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"120"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"85"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"31", "xml_views"=>"2", "html_views"=>"196"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"89"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"125"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"3", "html_views"=>"76"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"103"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"125"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"69"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"59"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"49"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"22", "xml_views"=>"4", "html_views"=>"35"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"3", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/876984"], "description"=>"<p>(A) Scheme of the photoconversion experiment designed to determine the source of Gag molecules recruited to an assembly site. Recruitment can either occur from the surrounding plasma membrane (I) or directly from the cytosol (II). By calculating the ratio of fluorescence intensities in green and red channels at the assembly site with respect to the same ratio calculated from the local background, the origin of the recruited Gag molecules could be determined. (B–C) Live-cell images of a HeLa cell transfected with a mixture of pCHIV andpCHIV<sup>mEosFP</sup> before photoconversion (B) and directly after photoconversion (C). The scale bar signifies 5 µm. (D) Intensity trajectory from an individual Gag cluster that appeared 26 s after photoconversion. (E) The average fluorescence intensity with 488 nm excitation (green line) and with 561 nm excitation (red line) of the plasma membrane measured in the vicinity of 23 assembly sites that were detectable before photoconversion as a function of time after photoconversion. The standard deviation of the individual traces is given as a halo. (F) The ratio of fluorescence intensity after green excitation to the fluorescence signal after red excitation normalized to the same ratio determined from the local background measured for 102 different assembly sites in five cells.</p>", "links"=>[], "tags"=>["gag", "budding"], "article_id"=>547437, "categories"=>["Infectious Diseases", "Virology"], "users"=>["Sergey Ivanchenko", "William J. Godinez", "Marko Lampe", "Hans-Georg Kräusslich", "Roland Eils", "Karl Rohr", "Christoph Bräuchle", "Barbara Müller", "Don C. Lamb"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000652.g005", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Recruitment_of_Gag_to_the_budding_site_/547437", "title"=>"Recruitment of Gag to the budding site.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-11-06 02:03:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/876887"], "description"=>"<p>Assembly rates were measured at different time intervals after appearance of the first assembly sites. (A) Average fluorescence intensity traces of clusters which appeared between 0 and 25 minutes (<i>upper panel</i>) and 40–60 minutes (<i>lower panel</i>) after the onset of virus production on the surface of a single cell. (B) background in WF and TIRF channels (<i>upper panel</i>) indicating the concentration of Gag in the cytosol and plasma membrane respectively and ratio of the fluorescent background in TIRF and WF (<i>lower panel</i>). The grey halo in the lower panel shows the standard deviation of the data. (C) Rates of assembly versus time of first detection for three individual cells (<i>left panel</i>) and histogram summarizing all rates from the same three cells (<i>right panel</i>). The black line shows a log-normal fit yielding an average rate of (5.5±0.5)×10<sup>−3</sup> s<sup>−1</sup>.</p>", "links"=>[], "tags"=>["gag"], "article_id"=>547335, "categories"=>["Infectious Diseases", "Virology"], "users"=>["Sergey Ivanchenko", "William J. Godinez", "Marko Lampe", "Hans-Georg Kräusslich", "Roland Eils", "Karl Rohr", "Christoph Bräuchle", "Barbara Müller", "Don C. Lamb"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000652.g004", "stats"=>{"downloads"=>1, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rates_of_Gag_assembly_versus_time_/547335", "title"=>"Rates of Gag assembly versus time.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-11-06 02:02:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/876478"], "description"=>"<p>HeLa cell transfected with a mixture of pCHIV and pCHIV<sup>eGFP</sup> imaged at 25 h hpt (A) in WF and (B) in TIRF mode. The scale bar represents 5 µm. The arrow and the arrow head indicate the two individual punctae analyzed in <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000652#ppat-1000652-g002\" target=\"_blank\">Figure 2A and B</a> respectively. (C), Normalized histograms of fluorescence intensities from Gag punctae on the cell surface (upper panel, shown in hatched bars with different colors representing different cells) and released particles that adhered to the coverslip after cell retraction during a live-cell imaging experiment (<i>lower panel</i>, <i>hatched bars</i>). For comparison, the fluorescence intensity distribution of a purified particle preparation is shown in grey bars in both panels (the normalized area of the histogram has been scaled by a factor of 2 for clarity).</p>", "links"=>[], "tags"=>["imaging", "hiv-1"], "article_id"=>546920, "categories"=>["Infectious Diseases", "Virology"], "users"=>["Sergey Ivanchenko", "William J. Godinez", "Marko Lampe", "Hans-Georg Kräusslich", "Roland Eils", "Karl Rohr", "Christoph Bräuchle", "Barbara Müller", "Don C. Lamb"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000652.g001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Live_cell_imaging_of_individual_HIV_1_assembly_sites_/546920", "title"=>"Live cell imaging of individual HIV-1 assembly sites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-11-06 01:55:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/435575", "https://ndownloader.figshare.com/files/435688", "https://ndownloader.figshare.com/files/435713", "https://ndownloader.figshare.com/files/435751", "https://ndownloader.figshare.com/files/435788", "https://ndownloader.figshare.com/files/435819", "https://ndownloader.figshare.com/files/435927", "https://ndownloader.figshare.com/files/435981", "https://ndownloader.figshare.com/files/436040", "https://ndownloader.figshare.com/files/436089", "https://ndownloader.figshare.com/files/436151", "https://ndownloader.figshare.com/files/436223", "https://ndownloader.figshare.com/files/436260", "https://ndownloader.figshare.com/files/436298", "https://ndownloader.figshare.com/files/436350", "https://ndownloader.figshare.com/files/436402"], "description"=>"<div><p>Assembly and release of human immunodeficiency virus (HIV) occur at the plasma membrane of infected cells and are driven by the Gag polyprotein. Previous studies analyzed viral morphogenesis using biochemical methods and static images, while dynamic and kinetic information has been lacking until very recently. Using a combination of wide-field and total internal reflection fluorescence microscopy, we have investigated the assembly and release of fluorescently labeled HIV-1 at the plasma membrane of living cells with high time resolution. Gag assembled into discrete clusters corresponding to single virions. Formation of multiple particles from the same site was rarely observed. Using a photoconvertible fluorescent protein fused to Gag, we determined that assembly was nucleated preferentially by Gag molecules that had recently attached to the plasma membrane or arrived directly from the cytosol. Both membrane-bound and cytosol derived Gag polyproteins contributed to the growing bud. After their initial appearance, assembly sites accumulated at the plasma membrane of individual cells over 1–2 hours. Assembly kinetics were rapid: the number of Gag molecules at a budding site increased, following a saturating exponential with a rate constant of ∼5×10<sup>−3</sup> s<sup>−1</sup>, corresponding to 8–9 min for 90% completion of assembly for a single virion. Release of extracellular particles was observed at ∼1,500±700 s after the onset of assembly. The ability of the virus to recruit components of the cellular ESCRT machinery or to undergo proteolytic maturation, or the absence of Vpu did not significantly alter the assembly kinetics.</p></div>", "links"=>[], "tags"=>["hiv-1"], "article_id"=>145905, "categories"=>["Cancer"], "users"=>["Sergey Ivanchenko", "William J. Godinez", "Marko Lampe", "Hans-Georg Kräusslich", "Roland Eils", "Karl Rohr", "Christoph Bräuchle", "Barbara Müller", "Don C. Lamb"], "doi"=>["https://dx.doi.org/10.1371/journal.ppat.1000652.s001", "https://dx.doi.org/10.1371/journal.ppat.1000652.s002", "https://dx.doi.org/10.1371/journal.ppat.1000652.s003", "https://dx.doi.org/10.1371/journal.ppat.1000652.s004", "https://dx.doi.org/10.1371/journal.ppat.1000652.s005", "https://dx.doi.org/10.1371/journal.ppat.1000652.s006", "https://dx.doi.org/10.1371/journal.ppat.1000652.s007", "https://dx.doi.org/10.1371/journal.ppat.1000652.s008", "https://dx.doi.org/10.1371/journal.ppat.1000652.s009", "https://dx.doi.org/10.1371/journal.ppat.1000652.s010", "https://dx.doi.org/10.1371/journal.ppat.1000652.s011", "https://dx.doi.org/10.1371/journal.ppat.1000652.s012", "https://dx.doi.org/10.1371/journal.ppat.1000652.s013", "https://dx.doi.org/10.1371/journal.ppat.1000652.s014", "https://dx.doi.org/10.1371/journal.ppat.1000652.s015", "https://dx.doi.org/10.1371/journal.ppat.1000652.s016"], "stats"=>{"downloads"=>29, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Dynamics_of_HIV_1_Assembly_and_Release/145905", "title"=>"Dynamics of HIV-1 Assembly and Release", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2009-11-06 01:38:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/877117"], "description"=>"<p>(A) Live-cell images of mCherry-labeled Gag in wide field (<i>left panel</i>) and TIRF (<i>middle panel</i>) as well as eGFP-GPI imaged in TIRF (<i>right panel</i>); the scale bar represents 10 µm. (B) The fluorescence intensity of eGFP-GPI observed in the green channel at the location of the tracked HIV<sup>mCherry</sup> assembly site (black line) and the background signal in the red channel derived from a region surrounding the tracked particle (grey line). The fluorescence intensity from the eGFP channel is divided by a factor of 4.5. Fluctuations of the plasma membrane in the TIRF evanescent field were detectable in both traces, validating usage of the Gag background signal as an indicator of membrane fluctuations in our analyses. (C) The background GPI signal (grey) increased over time indicating the delivery of new eGFP-GPI to the membrane. No enhancement of the eGFP-GPI signal (blue) was found at the individual HIV budding site, whereas Gag (red) increased.</p>", "links"=>[], "tags"=>["egfp-gpi", "hiv-1", "gag", "viral"], "article_id"=>547567, "categories"=>["Infectious Diseases", "Virology"], "users"=>["Sergey Ivanchenko", "William J. Godinez", "Marko Lampe", "Hans-Georg Kräusslich", "Roland Eils", "Karl Rohr", "Christoph Bräuchle", "Barbara Müller", "Don C. Lamb"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000652.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_the_dynamics_of_eGFP_GPI_and_HIV_1_Gag_during_viral_assembly_/547567", "title"=>"Comparison of the dynamics of eGFP-GPI and HIV-1 Gag during viral assembly.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-11-06 02:06:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/876729"], "description"=>"<p>The rates of processes involved in HIV<sup>eGFP</sup> assembly determined from single cells using TIRF microscopy. Shown are the averaged data (black line), the standard deviation of the individual traces (grey halo) and the fit of the average data to a saturating (Phase I, <i>left panels</i>) or decaying (Phase III, <i>right panels</i>) exponential (red line). Data are shown for 125 traces from a cell expressing wt HIV-1 (A), 89 traces from a ESCRT-recruitment defective variant displaying diminished release (B) and 69 traces from a maturation defective variant carrying a mutation in the PR active site (C). As the measurements were performed with different camera settings, the fluorescence intensities have been normalized to allow a direct comparison between experiments.</p>", "links"=>[], "tags"=>["kinetics", "phases"], "article_id"=>547179, "categories"=>["Infectious Diseases", "Virology"], "users"=>["Sergey Ivanchenko", "William J. Godinez", "Marko Lampe", "Hans-Georg Kräusslich", "Roland Eils", "Karl Rohr", "Christoph Bräuchle", "Barbara Müller", "Don C. Lamb"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000652.g003", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Average_kinetics_of_phases_I_and_III_/547179", "title"=>"Average kinetics of phases I and III.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-11-06 01:59:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/877213"], "description"=>"*<p>Not Determined.</p><p>Kinetic rate constants were determined from data collected using TIRFM or SDCM. The errors are the standard deviation calculated from measurements on multiple cells (4 cells for WT and PR- , 3 cells for the Late- variant and 2 cells for ΔVpu) and include the cell to cell variation in the rate of assembly. For measurements using SDCM, the error represents the standard error of the exponential fit.</p>", "links"=>[], "tags"=>["rates", "gag"], "article_id"=>547665, "categories"=>["Infectious Diseases", "Virology"], "users"=>["Sergey Ivanchenko", "William J. Godinez", "Marko Lampe", "Hans-Georg Kräusslich", "Roland Eils", "Karl Rohr", "Christoph Bräuchle", "Barbara Müller", "Don C. Lamb"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000652.t001", "stats"=>{"downloads"=>3, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Kinetic_rates_of_Gag_assembly_for_phase_I_and_phase_III_/547665", "title"=>"Kinetic rates of Gag assembly for phase I and phase III.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2009-11-06 02:07:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/876623"], "description"=>"<p>(A–B) Intensity traces of two individual Gag.eGFP clusters indicated with an arrow (A) and arrow head (B) in <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000652#ppat-1000652-g001\" target=\"_blank\">Figure 1</a> recorded in TIRF (black line) and WF (grey line) modes are shown in the <i>top panels</i>. Three phases, separated here by dashed lines, can be observed: Phase I) a rapid rise in fluorescence intensity, Phase II) a plateau region with fluctuations in fluorescence intensity, and Phase III) a decay of the fluorescence signal. The <i>middle panels</i> show the intensity traces of the local background. The <i>bottom panel</i> displays a plot of the corresponding instantaneous velocities of the particles. In A, an abrupt increase in instantaneous velocity is observed concomitantly with the onset of Phase III. (C) Trajectory of the individual Gag.eGFP cluster from panel A color-coded according to the three different phases. The scale bar represents 1 µm. (D) A mean-square displacement analysis of the trajectory shown in panel C indicates the random character of motion in the different phases and shows a change by two orders of magnitude in diffusion coefficient between the phases I+II and III.</p>", "links"=>[], "tags"=>["phases", "hiv-1"], "article_id"=>547071, "categories"=>["Infectious Diseases", "Virology"], "users"=>["Sergey Ivanchenko", "William J. Godinez", "Marko Lampe", "Hans-Georg Kräusslich", "Roland Eils", "Karl Rohr", "Christoph Bräuchle", "Barbara Müller", "Don C. Lamb"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000652.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_three_phases_of_HIV_1_assembly_/547071", "title"=>"The three phases of HIV-1 assembly.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2009-11-06 01:57:51"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"59", "unique-ip"=>"54", "pdf"=>"50", "year"=>"2010", "figure"=>"21", "scanned-summary"=>"0", "supp-data"=>"27"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"51", "year"=>"2010", "pdf"=>"38", "unique-ip"=>"48", "figure"=>"7", "scanned-summary"=>"0", "supp-data"=>"18"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"84", "unique-ip"=>"84", "pdf"=>"77", "year"=>"2010", "figure"=>"19", "scanned-summary"=>"0", "supp-data"=>"41"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"46", "year"=>"2010", "pdf"=>"28", "unique-ip"=>"43", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"21"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"5", "full-text"=>"40", "unique-ip"=>"45", "pdf"=>"33", "year"=>"2010", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"16"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"30", "year"=>"2010", "pdf"=>"32", "unique-ip"=>"31", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"4"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"32", "unique-ip"=>"26", "pdf"=>"15", "year"=>"2010", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"19", "year"=>"2010", "pdf"=>"14", "unique-ip"=>"19", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"4"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"14", "unique-ip"=>"18", "pdf"=>"13", "year"=>"2010", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"5"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"11", "year"=>"2010", "pdf"=>"7", "unique-ip"=>"14", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"22", "unique-ip"=>"19", "pdf"=>"9", "year"=>"2010", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"7"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"19", "year"=>"2010", "pdf"=>"7", "unique-ip"=>"10", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"1", "abstract"=>"1", "full-text"=>"15", "unique-ip"=>"15", "pdf"=>"12", "year"=>"2011", "figure"=>"4", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"22", "year"=>"2011", "pdf"=>"8", "unique-ip"=>"21", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"3", "full-text"=>"17", "unique-ip"=>"22", "pdf"=>"19", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"34", "year"=>"2011", "pdf"=>"13", "unique-ip"=>"18", "figure"=>"14", "scanned-summary"=>"0", "supp-data"=>"8"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"6", "unique-ip"=>"8", "pdf"=>"2", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"2", "year"=>"2011", "pdf"=>"2", "unique-ip"=>"6", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"19", "unique-ip"=>"16", "pdf"=>"6", "year"=>"2011", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"15", "year"=>"2011", "pdf"=>"7", "unique-ip"=>"15", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"16"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"6", "unique-ip"=>"8", "pdf"=>"6", "year"=>"2011", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"12", "year"=>"2011", "pdf"=>"9", "unique-ip"=>"12", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"17", "unique-ip"=>"15", "pdf"=>"1", "year"=>"2011", "figure"=>"5", "scanned-summary"=>"0", "supp-data"=>"12"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"2", "year"=>"2011", "pdf"=>"0", "unique-ip"=>"3", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"11", "unique-ip"=>"11", "pdf"=>"4", "year"=>"2012", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"1", "abstract"=>"0", "full-text"=>"9", "year"=>"2012", "pdf"=>"5", "unique-ip"=>"12", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"1"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"1", "abstract"=>"0", "full-text"=>"10", "unique-ip"=>"11", "pdf"=>"6", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"20", "year"=>"2012", "pdf"=>"13", "unique-ip"=>"19", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"7", "unique-ip"=>"8", "pdf"=>"4", "year"=>"2012", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"12"}
  • {"month"=>"11", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"8", "full-text"=>"178", "year"=>"2009", "pdf"=>"98", "unique-ip"=>"151", "figure"=>"34", "scanned-summary"=>"0", "supp-data"=>"33"}
  • {"scanned-page-browse"=>"0", "month"=>"12", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"86", "unique-ip"=>"73", "pdf"=>"58", "year"=>"2009", "figure"=>"23", "scanned-summary"=>"0", "supp-data"=>"17"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"5", "year"=>"2012", "pdf"=>"6", "unique-ip"=>"9", "figure"=>"3", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"41", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"22", "full-text"=>"17", "pdf"=>"13", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"41", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"17", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"15", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"45", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"35", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"6", "cited-by"=>"1", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"29", "full-text"=>"21", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"20", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2009-01-01T00:00:00Z", "end_date"=>"2009-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[337, 605, 735, 844, 939, 1037, 1139, 1214, 1300, 1375, 1461, 1543, 1619, 1693, 1768, 1827, 1881, 1948, 2013, 2066, 2118, 2172, 2227, 2270, 2325, 2386, 2443, 2504, 2565, 2609, 2659, 2731, 2792, 2857, 2920, 2984, 3051, 3101, 3165, 3213, 3276, 3344, 3397, 3462, 3512, 3561, 3629, 3710, 3779, 3847, 3897, 3949, 4013, 4086, 4140, 4180, 4239, 4288, 4344, 4409, 4470]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[332, 584, 719, 824, 927, 1030, 1139, 1233, 1322, 1402, 1483, 1570, 1649, 1730, 1806, 1873, 1939, 2003, 2068, 2130, 2191, 2248, 2302, 2362, 2427, 2485, 2544, 2599, 2653, 2709, 2778, 2840, 2896, 2950, 3013, 3073, 3137, 3189, 3253, 3305, 3364, 3423, 3489, 3557, 3606, 3668, 3742, 3797, 3852, 3907, 3962, 4027, 4072, 4132, 4193, 4256, 4308, 4366, 4427, 4493, 4556]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[302, 565, 710, 814, 921, 1026, 1126, 1222, 1311, 1409, 1491, 1580, 1659, 1736, 1809, 1874, 1947, 2004, 2061, 2133, 2186, 2236, 2302, 2362, 2441, 2494, 2550, 2603, 2651, 2711, 2785, 2840, 2904, 2962, 3018, 3066, 3133, 3181, 3253, 3300, 3357, 3409, 3459, 3556, 3621, 3657, 3709, 3784, 3856, 3886, 3958, 4027, 4081, 4124, 4183, 4241, 4297, 4338, 4391, 4437, 4488]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[340, 575, 698, 803, 889, 974, 1077, 1169, 1245, 1318, 1398, 1468, 1540, 1608, 1679, 1748, 1819, 1877, 1937, 1999, 2044, 2100, 2148, 2198, 2250, 2306, 2357, 2414, 2458, 2517, 2572, 2636, 2690, 2749, 2799, 2861, 2914, 2970, 3026, 3102, 3143, 3237, 3303, 3373, 3441, 3500, 3553, 3608, 3679, 3733, 3791, 3856, 3908, 3962, 4033, 4096, 4149, 4187, 4229, 4281, 4336]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[332, 576, 695, 791, 879, 956, 1066, 1171, 1247, 1317, 1390, 1466, 1562, 1625, 1681, 1751, 1807, 1879, 1927, 1990, 2050, 2099, 2141, 2186, 2236, 2295, 2337, 2370, 2434, 2503, 2563, 2634, 2685, 2738, 2802, 2853, 2929, 2980, 3024, 3073, 3135, 3214, 3271, 3354, 3411, 3479, 3542, 3610, 3665, 3722, 3759, 3799, 3872, 3957, 3997, 4051, 4121, 4197, 4241, 4292, 4321]}]}
Loading … Spinner
There are currently no alerts