Tsetse EP Protein Protects the Fly Midgut from Trypanosome Establishment
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Mendeley | Further Information

{"title"=>"Tsetse EP protein protects the fly midgut from trypanosome establishment", "type"=>"journal", "authors"=>[{"first_name"=>"Lee R.", "last_name"=>"Haines", "scopus_author_id"=>"7004167544"}, {"first_name"=>"Stella M.", "last_name"=>"Lehane", "scopus_author_id"=>"6603278726"}, {"first_name"=>"Terry W.", "last_name"=>"Pearson", "scopus_author_id"=>"7202069290"}, {"first_name"=>"Michael J.", "last_name"=>"Lehane", "scopus_author_id"=>"7005720726"}], "year"=>2010, "source"=>"PLoS Pathogens", "identifiers"=>{"issn"=>"15537366", "scopus"=>"2-s2.0-77950441667", "pui"=>"358569038", "doi"=>"10.1371/journal.ppat.1000793", "isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "sgr"=>"77950441667", "pmid"=>"20221444"}, "id"=>"8e777335-d27c-3584-aed9-ecddb7d42f71", "abstract"=>"African trypanosomes undergo a complex developmental process in their tsetse fly vector before transmission back to a vertebrate host. Typically, 90% of fly infections fail, most during initial establishment of the parasite in the fly midgut. The specific mechanism(s) underpinning this failure are unknown. We have previously shown that a Glossina-specific, immunoresponsive molecule, tsetse EP protein, is up regulated by the fly in response to gram-negative microbial challenge. Here we show by knockdown using RNA interference that this tsetse EP protein acts as a powerful antagonist of establishment in the fly midgut for both Trypanosoma brucei brucei and T. congolense. We demonstrate that this phenomenon exists in two species of tsetse, Glossina morsitans morsitans and G. palpalis palpalis, suggesting tsetse EP protein may be a major determinant of vector competence in all Glossina species. Tsetse EP protein levels also decline in response to starvation of the fly, providing a possible explanation for increased susceptibility of starved flies to trypanosome infection. As starvation is a common field event, this fact may be of considerable importance in the epidemiology of African trypanosomiasis.", "link"=>"http://www.mendeley.com/research/tsetse-ep-protein-protects-fly-midgut-trypanosome-establishment", "reader_count"=>52, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>5, "Researcher"=>13, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>4, "Student > Master"=>10, "Other"=>1, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>5, "Researcher"=>13, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>4, "Student > Master"=>10, "Other"=>1, "Student > Bachelor"=>5, "Professor"=>1}, "reader_count_by_subject_area"=>{"Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>8, "Agricultural and Biological Sciences"=>39, "Medicine and Dentistry"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>39}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>8}, "Environmental Science"=>{"Environmental Science"=>1}}, "reader_count_by_country"=>{"Czech Republic"=>1, "United States"=>3, "South Africa"=>1, "United Kingdom"=>1, "Germany"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/859655"], "description"=>"<p>The top panel is a schematic of the tsetse EP protein. SP  =  signal peptide. R AGT indicates the N-terminus (tryptic cleavage site) resulting in the mature protein. The dark blue represents the mature protein and the highlight red region (bracketed with arrows) indicates the target region for RNAi. In the sequence below, the predicted signal peptide is shown in lower case. |A indicates the N-terminus (tryptic cleavage site) resulting in the mature protein. The grey highlighted section indicates the region amplified for RNA interference. The residues in yellow indicate sequence differences in the <i>G. p. palpalis</i> sequence when compared with the sequence of <i>G. m. morsitans</i>. The monoclonal antibody, mAb 247, will recognize EPEPEP, and thus will bind along the entire length of the C-terminal rich region (E<sub>195</sub>–P<sub>306</sub>). S* indicates potential O-glycosylation sites.</p>", "links"=>[], "tags"=>["tsetse", "ep"], "article_id"=>530117, "categories"=>["Immunology"], "users"=>["Lee R. Haines", "Stella M. Lehane", "Terry W. Pearson", "Michael J. Lehane"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000793.g002", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_G_m_morsitans_tsetse_EP_protein_/530117", "title"=>"<i>G. m. morsitans</i> tsetse EP protein.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 02:21:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/859753"], "description"=>"<p>Teneral flies were injected with dsRNA, fed two bloodmeals and then starved for three days prior to dissection. Five midguts were pooled for each sample and the quantity of RNA and protein per lane was adjusted accordingly. (Panel A) Northern blot: lane 1: ds<i>tsetse EP</i> knockdown midguts; lane 2: NFW injected midguts; lane 3: control midgut tissue from non-injected flies. The lower section represents GAPDH mRNA loading controls. (Panel B) Immunoblot: Gel-separated midgut proteins (1/2 midgut equivalent) isolated from male <i>G. m. morsitans</i> injected with 2 µL of dsRNA were separated on a 12.5% gel and blotted with mAb 247 (anti-EP repeat). Lane 1: NFW control, lane 2: 8 µg ds<i>Amp</i> control, lane 3: 2 µg ds<i>tsetseEP</i>, lane 4: 4 µg ds<i>tsetseEP</i>, lane 5: 6 µg ds<i>tsetseEP</i>, lane 6: 7 µg ds<i>tsetseEP</i>, lane 7: 8 µg ds<i>tsetseEP</i>, lane 8: 10 kDa molecular mass ladder, lane 9: 10 µg ds<i>tsetseEP</i>.</p>", "links"=>[], "tags"=>["ep", "mrna", "levels", "injection", "tsetse"], "article_id"=>530206, "categories"=>["Immunology"], "users"=>["Lee R. Haines", "Stella M. Lehane", "Terry W. Pearson", "Michael J. Lehane"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000793.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tsetse_EP_mRNA_and_protein_levels_following_injection_of_tsetse_with_ds_tsetseEP_/530206", "title"=>"Tsetse EP mRNA and protein levels following injection of tsetse with ds<i>tsetseEP</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 02:21:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/859564"], "description"=>"<p>(A) Photograph of tsetse midgut depicting dissection lines. (B) Transcript profiles of midgut sections probed with tsetse EP and GAPDH (control) primers. (C) Immunoblot of midgut tissues (collected from a pool of five male <i>G. m. morsitans</i>) using mAb 247 (anti-EP repeat). PV  =  proventriculus; A1  =  midgut anterior to the bacteriome; Y  =  bacteriome; A2  =  anterior midgut posterior to the bacteriome; M  =  mid-midgut; P  =  posterior midgut.</p>", "links"=>[], "tags"=>["tsetse", "ep", "transcripts", "proteins", "sectioned", "midguts", "semi-quantitative", "rt-pcr"], "article_id"=>530011, "categories"=>["Immunology"], "users"=>["Lee R. Haines", "Stella M. Lehane", "Terry W. Pearson", "Michael J. Lehane"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000793.g001", "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Levels_of_tsetse_EP_transcripts_and_proteins_in_sectioned_midguts_were_determined_by_semi_quantitative_RT_PCR_and_immunoblotting_/530011", "title"=>"Levels of tsetse EP transcripts and proteins in sectioned midguts were determined by semi-quantitative RT-PCR and immunoblotting.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 02:20:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/859826"], "description"=>"<p>There is a decline in tsetse EP protein levels in midguts over a 7 day starvation period following the 5<sup>th</sup> bloodmeal. 24 h  =  Flies starved for 7 days, fed a blood meal and then sacrificed 24 hours later. L  =  molecular mass ladder. Midgut proteins (1/2 midgut equivalent from pool of 5) were blotted with mAb 247.</p>", "links"=>[], "tags"=>["lies", "nigrosine-stained"], "article_id"=>530286, "categories"=>["Immunology"], "users"=>["Lee R. Haines", "Stella M. Lehane", "Terry W. Pearson", "Michael J. Lehane"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000793.g004", "stats"=>{"downloads"=>2, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunoblot_film_lies_below_the_nigrosine_stained_PVDF_/530286", "title"=>"Immunoblot film lies below the nigrosine-stained PVDF.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-21 02:22:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/859892"], "description"=>"<p>Controls were either ds<i>Ampicillin</i>, ds<i>eGFP</i> or nuclease free water*. Flies were infected with either <i>T. b. brucei</i> TSW196 or <i>T. congolense</i> 1/148 (italics) blood stream forms in the indicated bloodmeal.</p>", "links"=>[], "tags"=>["prevalence", "midgut", "infections", "rnai", "knockdown"], "article_id"=>530345, "categories"=>["Immunology"], "users"=>["Lee R. Haines", "Stella M. Lehane", "Terry W. Pearson", "Michael J. Lehane"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1000793.t001", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mean_S_E_prevalence_of_midgut_infections_in_male_G_m_morsitans_Gmm_or_G_p_palpalis_Gpp_after_RNAi_knockdown_using_ds_tsetse_EP_/530345", "title"=>"Mean (± S.E.) prevalence of midgut infections in male <i>G. m. morsitans</i> (<i>Gmm</i>) or <i>G. p. palpalis</i> (<i>Gpp</i>) after RNAi knockdown using ds<i>tsetse EP</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-21 02:22:22"}

PMC Usage Stats | Further Information

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