Transition of Plasmodium Sporozoites into Liver Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio", "type"=>"journal", "authors"=>[{"first_name"=>"Carina S.S.", "last_name"=>"Gomes-Santos", "scopus_author_id"=>"8207955200"}, {"first_name"=>"Joanna", "last_name"=>"Braks", "scopus_author_id"=>"6602653132"}, {"first_name"=>"Miguel", "last_name"=>"Prudêncio", "scopus_author_id"=>"6603561872"}, {"first_name"=>"Céline", "last_name"=>"Carret", "scopus_author_id"=>"9036061300"}, {"first_name"=>"Ana Rita", "last_name"=>"Gomes", "scopus_author_id"=>"57195408999"}, {"first_name"=>"Arnab", "last_name"=>"Pain", "scopus_author_id"=>"7004341451"}, {"first_name"=>"Theresa", "last_name"=>"Feltwell", "scopus_author_id"=>"6603435566"}, {"first_name"=>"Shahid", "last_name"=>"Khan", "scopus_author_id"=>"34770631600"}, {"first_name"=>"Andrew", "last_name"=>"Waters", "scopus_author_id"=>"7101758887"}, {"first_name"=>"Chris", "last_name"=>"Janse", "scopus_author_id"=>"7005097427"}, {"first_name"=>"Gunnar R.", "last_name"=>"Mair", "scopus_author_id"=>"7006355787"}, {"first_name"=>"Maria M.", "last_name"=>"Mota", "scopus_author_id"=>"7102709816"}], "year"=>2011, "source"=>"PLoS Pathogens", "identifiers"=>{"pui"=>"361895936", "isbn"=>"1553-7374 (Electronic) 1553-7366 (Linking)", "issn"=>"15537366", "doi"=>"10.1371/journal.ppat.1002046", "scopus"=>"2-s2.0-79958029441", "pmid"=>"21625527", "sgr"=>"79958029441"}, "id"=>"d3ac79a5-7d7a-3799-9f02-080787445a93", "abstract"=>"Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a Plasmodium member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. Puf2- sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early Plasmodium intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism.", "link"=>"http://www.mendeley.com/research/transition-plasmodium-sporozoites-liver-stagelike-forms-regulated-rna-binding-protein-pumilio", "reader_count"=>90, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>6, "Researcher"=>25, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>24, "Student > Postgraduate"=>5, "Student > Master"=>15, "Other"=>2, "Student > Bachelor"=>6, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>6, "Researcher"=>25, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>24, "Student > Postgraduate"=>5, "Student > Master"=>15, "Other"=>2, "Student > Bachelor"=>6, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>14, "Agricultural and Biological Sciences"=>64, "Medicine and Dentistry"=>5, "Chemistry"=>2, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>64}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>14}, "Unspecified"=>{"Unspecified"=>1}, "Environmental Science"=>{"Environmental Science"=>1}}, "reader_count_by_country"=>{"Pakistan"=>1, "United States"=>5, "United Kingdom"=>2, "Portugal"=>3, "India"=>1}, "group_count"=>3}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79958029441"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/79958029441?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79958029441&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=79958029441&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/79958029441", "dc:identifier"=>"SCOPUS_ID:79958029441", "eid"=>"2-s2.0-79958029441", "dc:title"=>"Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio", "dc:creator"=>"Gomes-Santos C.S.S.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"7", "prism:issueIdentifier"=>"5", "prism:pageRange"=>nil, "prism:coverDate"=>"2011-05-01", "prism:coverDisplayDate"=>"May 2011", "prism:doi"=>"10.1371/journal.ppat.1002046", "citedby-count"=>"49", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Universidade de Coimbra, Centro De Neurociências e Biologia Celular", "affiliation-city"=>"Coimbra", "affiliation-country"=>"Portugal"}, {"@_fa"=>"true", "affilname"=>"University of Lisbon Faculty of Medicine, Institute of Molecular Medicine", "affiliation-city"=>"Lisbon", "affiliation-country"=>"Portugal"}], "pubmed-id"=>"21625527", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1002046", "source-id"=>"4000151809", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplospathogens%2Farticle%3Fid%3D10.1371%252Fjournal.ppat.1002046", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"5", "year"=>"2011", "pdf_views"=>"85", "xml_views"=>"3", "html_views"=>"333"}
  • {"month"=>"6", "year"=>"2011", "pdf_views"=>"83", "xml_views"=>"6", "html_views"=>"238"}
  • {"month"=>"7", "year"=>"2011", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"8", "year"=>"2011", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"73"}
  • {"month"=>"9", "year"=>"2011", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"10", "year"=>"2011", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"11", "year"=>"2011", "pdf_views"=>"26", "xml_views"=>"5", "html_views"=>"70"}
  • {"month"=>"12", "year"=>"2011", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"1", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"2", "year"=>"2012", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"3", "year"=>"2012", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"4", "year"=>"2012", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"77"}
  • {"month"=>"5", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"81"}
  • {"month"=>"6", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"7", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"13", "xml_views"=>"8", "html_views"=>"59"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"219"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"83"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"125"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"124"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"170"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"167"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"175"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"175"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"157"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"125"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"142"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"200"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"176"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"98"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"57"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"3", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"7"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/774227"], "description"=>"<p>Transmission electron microsocopy of 29-day salivary gland parasites\n showing the presence of slender-shaped, wild type and\n <i>puf1-</i> sporozoites with outer plasma membrane (PM)\n and entirely intact, inner membrane complex (IMC).\n <i>puf2-</i> salivary gland parasites developing into\n early EEFs show clear IMC disruption in the bulging region (arrows).\n Left pictures: longitudinal sections; right picture: transversal\n sections. Scale bars = 1 µm.</p>", "links"=>[], "tags"=>["parasites", "transforming", "eefs", "mosquito", "salivary"], "article_id"=>444576, "categories"=>["Molecular Biology", "Infectious Diseases"], "users"=>["Carina S. S. Gomes-Santos", "Joanna Braks", "Miguel Prudêncio", "Celine Carret", "Ana Rita Gomes", "Arnab Pain", "Theresa Feltwell", "Shahid Khan", "Andrew Waters", "Chris Janse", "Gunnar R. Mair", "Maria M. Mota"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002046.g005", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ultrastructural_evidence_of_puf2_parasites____transforming_into_early_liver_stage_EEFs_in_A_____stephensi_mosquito_salivary_glands_/444576", "title"=>"Ultrastructural evidence of <i>puf2-</i> parasites\n transforming into early liver stage EEFs in <i>A.\n stephensi</i> mosquito salivary glands.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-19 01:16:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/774482"], "description"=>"<p>In the mosquito Puf2 controls the translation of key protein(s). In its\n absence, this(ese) proteins are translated and sporozoites initiate\n transformation into the round EEF, which only occurs in the mammalian host\n in wild-type parasites.</p>", "links"=>[], "tags"=>["puf2-mediated", "of", "progression", "during", "mosquito", "vector", "mammalian"], "article_id"=>444838, "categories"=>["Molecular Biology", "Infectious Diseases"], "users"=>["Carina S. S. Gomes-Santos", "Joanna Braks", "Miguel Prudêncio", "Celine Carret", "Ana Rita Gomes", "Arnab Pain", "Theresa Feltwell", "Shahid Khan", "Andrew Waters", "Chris Janse", "Gunnar R. Mair", "Maria M. Mota"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002046.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proposed_model_for_Puf2_mediated_regulation_of____Plasmodium_developmental_progression_during____transmission_from_the_mosquito_vector_to_the_mammalian_host_/444838", "title"=>"Proposed model for Puf2-mediated regulation of\n <i>Plasmodium</i> developmental progression during\n transmission from the mosquito vector to the mammalian host.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-19 01:20:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/773791"], "description"=>"<p><b>A</b>, maximum projection of a z-series scan of infected\n mosquito salivary glands on day 30 of mosquito infection.\n <b>B</b>, Development of salivary gland-resident parasites from\n day 18 to day 30 after mosquito infection; <i>puf2-</i>\n undergo exo-erythrocytic liver stage metamorphosis with bulging visible\n at day 22 and complete transformation at day 30. <b>C</b>,\n Proportion of sporozoites versus EEF-like's of wild type,\n <i>puf1-</i> and <i>puf2-</i> parasites in\n mosquito SG's during 13 days of salivary gland infection. Scale\n bars = 10 µm.</p>", "links"=>[], "tags"=>["parasites", "stage", "mosquito", "salivary"], "article_id"=>444145, "categories"=>["Molecular Biology", "Infectious Diseases"], "users"=>["Carina S. S. Gomes-Santos", "Joanna Braks", "Miguel Prudêncio", "Celine Carret", "Ana Rita Gomes", "Arnab Pain", "Theresa Feltwell", "Shahid Khan", "Andrew Waters", "Chris Janse", "Gunnar R. Mair", "Maria M. Mota"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002046.g002", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_puf2_parasites_transform_into_early_liver_stage____EEF_s_in_mosquito_salivary_glands_/444145", "title"=>"<i>puf2-</i> parasites transform into early liver stage\n EEF's in mosquito salivary glands.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-19 01:09:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/774388"], "description"=>"<p><b>A,</b> Wild type 18 day SGS were incubated at 37°C or room\n temperature (RT) for 2 h and 4 h and with or without the presence of\n cycloheximide (CHD, 100 µg/ml) as indicated. Transformation of WT\n SGS into EEF-like <i>in vitro</i> is more pronounced at\n 37°C for 4 h and relies on protein translation. T-test **\n p<0.01. All data show mean±SD. <b>B,</b> WT and\n <i>puf2-</i> 18 day SGS were incubated at 37°C for 4 h\n with and without the presence of cycloheximide (CHD, 100 µg/ml) as\n indicated, showing that transformation of <i>puf2-</i>\n sporozoites does not rely on protein translation. <b>C,</b>\n Protein expression in <i>puf2-</i> and wild type SGS, 18 days\n after infection.</p>", "links"=>[], "tags"=>["sporozoite", "eef-like"], "article_id"=>444747, "categories"=>["Molecular Biology", "Infectious Diseases"], "users"=>["Carina S. S. Gomes-Santos", "Joanna Braks", "Miguel Prudêncio", "Celine Carret", "Ana Rita Gomes", "Arnab Pain", "Theresa Feltwell", "Shahid Khan", "Andrew Waters", "Chris Janse", "Gunnar R. Mair", "Maria M. Mota"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002046.g006", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Plasmodium_sporozoite_transformation_into_EEF_like____form_is_protein_translation_dependent_/444747", "title"=>"<i>Plasmodium</i> sporozoite transformation into EEF-like\n form is protein translation dependent.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-19 01:19:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/388390", "https://ndownloader.figshare.com/files/388423", "https://ndownloader.figshare.com/files/388447", "https://ndownloader.figshare.com/files/388472", "https://ndownloader.figshare.com/files/388499", "https://ndownloader.figshare.com/files/388522", "https://ndownloader.figshare.com/files/388546", "https://ndownloader.figshare.com/files/388590", "https://ndownloader.figshare.com/files/388623", "https://ndownloader.figshare.com/files/388654", "https://ndownloader.figshare.com/files/388689", "https://ndownloader.figshare.com/files/388735", "https://ndownloader.figshare.com/files/388780", "https://ndownloader.figshare.com/files/388800", "https://ndownloader.figshare.com/files/388850", "https://ndownloader.figshare.com/files/388880", "https://ndownloader.figshare.com/files/388907", "https://ndownloader.figshare.com/files/388945", "https://ndownloader.figshare.com/files/388980", "https://ndownloader.figshare.com/files/389016", "https://ndownloader.figshare.com/files/389050"], "description"=>"<div><p>Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (<em>Plasmodium</em> spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host. Here we identify a <em>Plasmodium</em> member of the RNA binding protein family PUF as a key regulator of this transformation. In the absence of Pumilio-2 (Puf2) sporozoites initiate EEF development inside mosquito salivary glands independently of the normal transmission-associated environmental cues. <em>Puf2-</em> sporozoites exhibit genome-wide transcriptional changes that result in loss of gliding motility, cell traversal ability and reduction in infectivity, and, moreover, trigger metamorphosis typical of early <em>Plasmodium</em> intra-hepatic development. These data demonstrate that Puf2 is a key player in regulating sporozoite developmental control, and imply that transformation of salivary gland-resident sporozoites into liver stage-like parasites is regulated by a post-transcriptional mechanism.</p> </div>", "links"=>[], "tags"=>["sporozoites", "liver", "stage-like", "forms", "regulated", "rna", "binding", "pumilio"], "article_id"=>136700, "categories"=>["Molecular Biology", "Cancer"], "users"=>["Carina S. S. Gomes-Santos", "Joanna Braks", "Miguel Prudêncio", "Celine Carret", "Ana Rita Gomes", "Arnab Pain", "Theresa Feltwell", "Shahid Khan", "Andrew Waters", "Chris Janse", "Gunnar R. Mair", "Maria M. Mota"], "doi"=>["https://dx.doi.org/10.1371/journal.ppat.1002046.s001", "https://dx.doi.org/10.1371/journal.ppat.1002046.s002", "https://dx.doi.org/10.1371/journal.ppat.1002046.s003", "https://dx.doi.org/10.1371/journal.ppat.1002046.s004", "https://dx.doi.org/10.1371/journal.ppat.1002046.s005", "https://dx.doi.org/10.1371/journal.ppat.1002046.s006", "https://dx.doi.org/10.1371/journal.ppat.1002046.s007", "https://dx.doi.org/10.1371/journal.ppat.1002046.s008", "https://dx.doi.org/10.1371/journal.ppat.1002046.s009", "https://dx.doi.org/10.1371/journal.ppat.1002046.s010", "https://dx.doi.org/10.1371/journal.ppat.1002046.s011", "https://dx.doi.org/10.1371/journal.ppat.1002046.s012", "https://dx.doi.org/10.1371/journal.ppat.1002046.s013", "https://dx.doi.org/10.1371/journal.ppat.1002046.s014", "https://dx.doi.org/10.1371/journal.ppat.1002046.s015", "https://dx.doi.org/10.1371/journal.ppat.1002046.s016", "https://dx.doi.org/10.1371/journal.ppat.1002046.s017", "https://dx.doi.org/10.1371/journal.ppat.1002046.s018", "https://dx.doi.org/10.1371/journal.ppat.1002046.s019", "https://dx.doi.org/10.1371/journal.ppat.1002046.s020", "https://dx.doi.org/10.1371/journal.ppat.1002046.s021"], "stats"=>{"downloads"=>6, "page_views"=>30, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Transition_of_Plasmodium_Sporozoites_into_Liver___Stage_Like_Forms_Is_Regulated_by_the_RNA_Binding_Protein_Pumilio/136700", "title"=>"Transition of <em>Plasmodium</em> Sporozoites into Liver\n Stage-Like Forms Is Regulated by the RNA Binding Protein Pumilio", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-05-19 01:51:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/774041"], "description"=>"<p><b>A</b>, Quantitative RT-PCR analysis was done on cDNA from wild\n type and <i>puf2-</i> salivary gland sporozoites (SGS) at day\n 18 after mosquito infection. <b>B</b>, Heatmap of expression\n changes measured by microarray analysis for >300 genes in wild type\n and <i>puf2-</i> at days 18 and 27 after mosquito infection.\n Expression values are scaled up to the rows and range from -3 (blue) to\n +3 (red). <b>C</b>, Correlation analysis of quantitative\n RT-PCR and microarray results.</p>", "links"=>[], "tags"=>["changes"], "article_id"=>444394, "categories"=>["Molecular Biology", "Infectious Diseases"], "users"=>["Carina S. S. Gomes-Santos", "Joanna Braks", "Miguel Prudêncio", "Celine Carret", "Ana Rita Gomes", "Arnab Pain", "Theresa Feltwell", "Shahid Khan", "Andrew Waters", "Chris Janse", "Gunnar R. Mair", "Maria M. Mota"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002046.g004", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptional_changes_in_puf2_/444394", "title"=>"Transcriptional changes in <i>puf2-</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-19 01:13:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/773924"], "description"=>"<p><b>A</b>, 3×10<sup>4</sup> sporozoites were allowed to glide\n on cover slips pre-coated with anti-circumsporozoite protein (CSP)\n antibody for 40 minutes at 37°C. The number of parasites associated\n with CSP trails is a measure of gliding motility ability.\n <b>B</b>, Dextran tetramethylrhodamine was added to Huh7 cells\n before sporozoite addition. Two hours p.i. the number of traversed cells\n (Dextran+) was quantified by fluorescence-activated cell sorting.\n <b>C</b>, Huh7 cells fixed and double-stained with anti-CSP\n antibody to distinguish extracellular from intracellular sporozoites\n after a 2-hour incubation with 3×10<sup>4</sup> sporozoites.\n <b>D</b>, EEF development 48 h after addition of sporozoites\n to Huh7 cells. EEF areas were quantified with Image J of fluorescence\n microscopy images. For <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002046#ppat-1002046-g003\" target=\"_blank\">Figure 3A-D</a>, n = 3. <b>E</b>,\n <i>Plasmodium</i> liver load 44 h after intravenous\n injection of 1×10<sup>4</sup> sporozoites; parasite load measured\n by qRT-PCR of <i>P. berghei</i> 18S rRNA normalized to\n hypoxanthine-guanine phosphoribosyltransferase. Five C57Bl/6 mice per\n group. <b>F</b>, Appearance of blood parasitaemia following\n infection of C57Bl/6 with 1×10<sup>4</sup> intravenously injected\n sporozoites (wild type n = 10 mice;\n <i>puf1-</i> n = 10;\n <i>puf2-</i> n = 25). <b>G</b>,\n Appearance of blood parasitaemia after mosquito bite (wild type\n n = 4 mice; <i>puf1-</i>\n n = 8; <i>puf2-</i>\n n = 10). All experiments used sporozoites 18 days\n after mosquito infection. T-test * p<0.05; ** p<0.01.\n All data show mean±SD.</p>", "links"=>[], "tags"=>["infectivity", "mutant"], "article_id"=>444278, "categories"=>["Molecular Biology", "Infectious Diseases"], "users"=>["Carina S. S. Gomes-Santos", "Joanna Braks", "Miguel Prudêncio", "Celine Carret", "Ana Rita Gomes", "Arnab Pain", "Theresa Feltwell", "Shahid Khan", "Andrew Waters", "Chris Janse", "Gunnar R. Mair", "Maria M. Mota"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002046.g003", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Functionality_and_liver_stage_infectivity_of_wild_type_and_mutant____sporozoites_/444278", "title"=>"Functionality and liver stage infectivity of wild type and mutant\n sporozoites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-19 01:11:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/773584"], "description"=>"<p><b>A</b>, Relative and stage-specific transcription levels of\n <i>puf1</i> and <i>puf2</i> in wild type\n parasites. <i>puf1</i> and <i>puf2</i> steady state\n mRNA levels were analyzed by RT-PCR using cDNA from highly purified\n gametocytes and day 18 salivary gland (SG) sporozoites. Genomic DNA\n amplification is also provided, together with a map showing the\n localisation of primers used for amplifying <i>puf1</i> and\n <i>puf2</i> and the different sizes to be expected in cDNA\n and genomic DNA. See also <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002046#ppat.1002046.s003\" target=\"_blank\">Fig. S3C</a> for Northern analysis of\n <i>puf2</i> throughout parasite life cycle.\n <b>B,</b> Sporozoites tagged with red fluorescent protein\n (line 733cl1) were stained with anti-Puf2 peptide antibody 904 (top\n panel) and 905 (lower panel) revealing distinct cytoplasmic protein\n speckles. Scale bars = 10 µm.</p>", "links"=>[], "tags"=>["mrna", "immunolocalization", "in", "salivary", "gland"], "article_id"=>443935, "categories"=>["Molecular Biology", "Infectious Diseases"], "users"=>["Carina S. S. Gomes-Santos", "Joanna Braks", "Miguel Prudêncio", "Celine Carret", "Ana Rita Gomes", "Arnab Pain", "Theresa Feltwell", "Shahid Khan", "Andrew Waters", "Chris Janse", "Gunnar R. Mair", "Maria M. Mota"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002046.g001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_puf_mRNA_expression_and_immunolocalization_in_____P_berghei_RFP_salivary_gland____sporozoites_/443935", "title"=>"<i>puf</i> mRNA expression and immunolocalization in\n <i>P. berghei</i> RFP+ salivary gland\n sporozoites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-05-19 01:05:35"}

PMC Usage Stats | Further Information

  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"1", "unique-ip"=>"2", "pdf"=>"3", "year"=>"2011", "figure"=>"0", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"82", "year"=>"2011", "pdf"=>"41", "unique-ip"=>"82", "figure"=>"53", "scanned-summary"=>"0", "supp-data"=>"30"}
  • {"scanned-page-browse"=>"0", "month"=>"7", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"36", "unique-ip"=>"35", "pdf"=>"23", "year"=>"2011", "figure"=>"23", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"8", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"26", "year"=>"2011", "pdf"=>"16", "unique-ip"=>"26", "figure"=>"16", "scanned-summary"=>"0", "supp-data"=>"12"}
  • {"scanned-page-browse"=>"0", "month"=>"9", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"31", "unique-ip"=>"31", "pdf"=>"18", "year"=>"2011", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"6"}
  • {"month"=>"10", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"24", "year"=>"2011", "pdf"=>"11", "unique-ip"=>"32", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"11", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"15", "unique-ip"=>"15", "pdf"=>"7", "year"=>"2011", "figure"=>"6", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"12", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"18", "year"=>"2011", "pdf"=>"11", "unique-ip"=>"21", "figure"=>"15", "scanned-summary"=>"0", "supp-data"=>"3"}
  • {"scanned-page-browse"=>"0", "month"=>"1", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"11", "unique-ip"=>"14", "pdf"=>"2", "year"=>"2012", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"month"=>"2", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"22", "year"=>"2012", "pdf"=>"14", "unique-ip"=>"24", "figure"=>"11", "scanned-summary"=>"0", "supp-data"=>"11"}
  • {"scanned-page-browse"=>"0", "month"=>"3", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"23", "unique-ip"=>"23", "pdf"=>"17", "year"=>"2012", "figure"=>"8", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"4", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"0", "full-text"=>"17", "year"=>"2012", "pdf"=>"5", "unique-ip"=>"14", "figure"=>"2", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"scanned-page-browse"=>"0", "month"=>"5", "cited-by"=>"0", "abstract"=>"1", "full-text"=>"23", "unique-ip"=>"21", "pdf"=>"9", "year"=>"2012", "figure"=>"10", "scanned-summary"=>"0", "supp-data"=>"2"}
  • {"month"=>"6", "scanned-page-browse"=>"0", "cited-by"=>"0", "abstract"=>"2", "full-text"=>"13", "year"=>"2012", "pdf"=>"8", "unique-ip"=>"15", "figure"=>"1", "scanned-summary"=>"0", "supp-data"=>"0"}
  • {"unique-ip"=>"19", "full-text"=>"24", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"23", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"41", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"27", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"24", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"24", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"24", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"22", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"21", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"22", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"27", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"21", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"30", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"21", "full-text"=>"19", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"22", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"24", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"29", "full-text"=>"36", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"24", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"27", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2011-01-01T00:00:00Z", "end_date"=>"2011-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[301, 558, 690, 806, 916, 1018, 1112, 1197, 1275, 1349, 1421, 1495, 1561, 1631, 1697, 1762, 1835, 1895, 1954, 2020, 2078, 2142, 2197, 2258, 2313, 2376, 2437, 2494, 2557, 2618, 2682, 2744, 2818, 2880, 2940, 3000, 3056]}, {"subject_area"=>"/Biology and life sciences/Anatomy and physiology", "average_usage"=>[298, 559, 690, 805, 916, 1018, 1115, 1201, 1283, 1356, 1425, 1498, 1565, 1635, 1703, 1767, 1835, 1902, 1962, 2027, 2089, 2150, 2210, 2265]}, {"subject_area"=>"/Biology and life sciences/Parasitology", "average_usage"=>[359, 649, 766, 882, 961, 1046, 1170, 1246, 1317, 1379, 1458, 1532, 1583, 1643, 1697, 1774, 1867, 1925, 1988, 2025, 2077, 2131, 2221, 2341, 2424, 2471, 2514, 2551, 2613, 2691, 2738, 2792, 2852, 2903, 2979, 3037, 3093]}, {"subject_area"=>"/Medicine and health sciences/Anatomy and physiology", "average_usage"=>[296, 554, 686, 802, 910, 1014, 1104, 1195, 1277, 1349, 1419, 1483, 1551, 1620, 1690, 1757, 1820, 1888, 1957, 2017, 2081, 2140, 2202, 2259]}, {"subject_area"=>"/Medicine and health sciences/Parasitic diseases", "average_usage"=>[362, 736, 861, 965, 1072, 1194, 1301, 1377, 1465, 1535, 1624, 1694, 1745, 1817, 1877, 1958, 2038, 2106, 2167, 2241, 2288, 2381, 2447, 2508, 2566, 2622, 2687, 2781, 2853, 2913, 2984, 3057, 3141, 3186, 3250, 3302, 3367]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[291, 540, 674, 787, 893, 986, 1087, 1172, 1249, 1319, 1394, 1465, 1526, 1588, 1645, 1713, 1774, 1837, 1903, 1959, 2027, 2085, 2152, 2205, 2259, 2318, 2377, 2436, 2486, 2539, 2603, 2664, 2719, 2779, 2835, 2888, 2944]}]}
Loading … Spinner
There are currently no alerts