Exon Level Transcriptomic Profiling of HIV-1-Infected CD4+ T Cells Reveals Virus-Induced Genes and Host Environment Favorable for Viral Replication
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Exon Level Transcriptomic Profiling of HIV-1-Infected CD4+ T Cells Reveals Virus-Induced Genes and Host Environment Favorable for Viral Replication", "type"=>"journal", "authors"=>[{"first_name"=>"Michaël", "last_name"=>"Imbeault", "scopus_author_id"=>"24166654400"}, {"first_name"=>"Katia", "last_name"=>"Giguère", "scopus_author_id"=>"38561382400"}, {"first_name"=>"Michel", "last_name"=>"Ouellet", "scopus_author_id"=>"8077780000"}, {"first_name"=>"Michel J.", "last_name"=>"Tremblay", "scopus_author_id"=>"55567133600"}], "year"=>2012, "source"=>"PLoS Pathogens", "identifiers"=>{"isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "pmid"=>"22876188", "doi"=>"10.1371/journal.ppat.1002861", "pui"=>"365631437", "issn"=>"15537366", "sgr"=>"84866172395", "scopus"=>"2-s2.0-84866172395"}, "id"=>"8c89f37c-3b21-3fb4-a007-54ca82ba5927", "abstract"=>"HIV-1 is extremely specialized since, even amongst CD4(+) T lymphocytes (its major natural reservoir in peripheral blood), the virus productively infects only a small proportion of cells under an activated state. As the percentage of HIV-1-infected cells is very low, most studies have so far failed to capture the precise transcriptomic profile at the whole-genome scale of cells highly susceptible to virus infection. Using Affymetrix Exon array technology and a reporter virus allowing the magnetic isolation of HIV-1-infected cells, we describe the host cell factors most favorable for virus establishment and replication along with an overview of virus-induced changes in host gene expression occurring exclusively in target cells productively infected with HIV-1. We also establish that within a population of activated CD4(+) T cells, HIV-1 has no detectable effect on the transcriptome of uninfected bystander cells at early time points following infection. The data gathered in this study provides unique insights into the biology of HIV-1-infected CD4(+) T cells and identifies genes thought to play a determinant role in the interplay between the virus and its host. Furthermore, it provides the first catalogue of alternative splicing events found in primary human CD4(+) T cells productively infected with HIV-1.", "link"=>"http://www.mendeley.com/research/exon-level-transcriptomic-profiling-hiv1infected-cd4-t-cells-reveals-virusinduced-genes-host-environ", "reader_count"=>47, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>5, "Researcher"=>14, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>4}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>5, "Researcher"=>14, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>3, "Professor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>22, "Medicine and Dentistry"=>7, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Sports and Recreations"=>1, "Social Sciences"=>1, "Immunology and Microbiology"=>6}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>7}, "Social Sciences"=>{"Social Sciences"=>1}, "Sports and Recreations"=>{"Sports and Recreations"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>6}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>22}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United States"=>2, "United Kingdom"=>2}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84866172395"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84866172395?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84866172395&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84866172395&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84866172395", "dc:identifier"=>"SCOPUS_ID:84866172395", "eid"=>"2-s2.0-84866172395", "dc:title"=>"Exon Level Transcriptomic Profiling of HIV-1-Infected CD4<sup>+</sup> T Cells Reveals Virus-Induced Genes and Host Environment Favorable for Viral Replication", "dc:creator"=>"Imbeault M.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"8", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-08-01", "prism:coverDisplayDate"=>"August 2012", "prism:doi"=>"10.1371/journal.ppat.1002861", "citedby-count"=>"26", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"CHUL Research Center", "affiliation-city"=>"Quebec", "affiliation-country"=>"Canada"}], "pubmed-id"=>"22876188", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1002861", "source-id"=>"4000151809", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplospathogens%2Farticle%3Fid%3D10.1371%252Fjournal.ppat.1002861", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"171", "xml_views"=>"16", "html_views"=>"895"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"69", "xml_views"=>"1", "html_views"=>"366"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"46", "xml_views"=>"0", "html_views"=>"177"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"35", "xml_views"=>"1", "html_views"=>"103"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"81"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"126"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"30", "xml_views"=>"2", "html_views"=>"297"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"103"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"115"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"105"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"198"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"154"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"142"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"3", "html_views"=>"173"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"131"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"158"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"109"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"3", "html_views"=>"105"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"179"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"241"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"131"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"61"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"220"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"57"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"6", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"13", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"4", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"6", "html_views"=>"8"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"0"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/597797"], "description"=>"<p>A) Design of the reporter virus used in this study. The murine reporter HSA gene ensures a rapid detection and isolation of cells productively infected with HIV-1. In this infectious molecular clone of HIV-1, the early viral gene <i>Nef</i> is expressed via an IRES sequence. The reporter virus thus expresses all viral genes and is fully replication competent. B) Flow cytometry analysis of the virus-infected cell populations. Left column: Percentage of HIV-1-infected primary human CD4<sup>+</sup> T cells at 24, 48 and 72 h post-infection, as revealed by HSA staining. Second, third and fourth columns: characterization of T cell phenotypes via CD45RO and CD27 staining for mock-infected, uninfected bystander (HSA<sup>−</sup>) and virus-infected cells (HSA<sup>+</sup>). C. Experimental design for the transcriptome analysis. Purified CD4<sup>+</sup> T cells (98% CD3<sup>+</sup>CD4<sup>+</sup>; data not shown) from the peripheral blood of three different donors were treated with PHA/rhIL-2 for two days, then either mock-infected or inoculated with NL4-3 BAL-IRES-HSA virus. Next, uninfected bystander cells (HSA<sup>−</sup>) and HIV-1-infected (HSA<sup>+</sup>) cell populations were separated through the use of magnetic beads. At least 200 ng of RNA were obtained from each cell population and processed for hybridization on microarrays. D) Purity levels attained after separation of the studied cell populations. qRT-PCR directed against spliced Tat mRNA was carried out to assess the presence of HIV-1-infected cells in each fraction (donors A, B and C). <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002861#s2\" target=\"_blank\">Results</a> are expressed relatively to virus-infected cell fractions, as the signal in negative controls is usually undetectable. Each value is normalized by the value of 18S ribosomal RNA. All HSA-positive cell fractions are highly enriched in virus-infected cells, whereas mock-infected and uninfected bystander cell populations contain virtually no detectable traces of viral transcripts.</p>", "links"=>[], "tags"=>["characterization", "hiv-1-infected"], "article_id"=>268283, "categories"=>["Genetics", "Infectious Diseases"], "users"=>["Michael Imbeault", "Katia Giguère", "Michel Ouellet", "Michel J. Tremblay"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002861.g001", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Isolation_and_characterization_of_HIV_1_infected_cells_/268283", "title"=>"Isolation and characterization of HIV-1-infected cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-02 02:18:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/598476"], "description"=>"<p>Expression levels of ZBED2, MYOF, GJB2, GJB6, TRIM22, CLC and DFNA5 were confirmed by qRT-PCR. The Pearson correlation coefficients indicate that the level of similarity between qRT-PCR quantification and microarrays is very high for all studied candidates.</p>", "links"=>[], "tags"=>["confirmation", "genes"], "article_id"=>268969, "categories"=>["Genetics", "Infectious Diseases"], "users"=>["Michael Imbeault", "Katia Giguère", "Michel Ouellet", "Michel J. Tremblay"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002861.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_qRT_PCR_confirmation_of_genes_of_interest_identified_in_the_dataset_/268969", "title"=>"qRT-PCR confirmation of genes of interest identified in the dataset.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-08-02 02:29:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/597867"], "description"=>"<p>A) Volcano plots from the limma analysis of the transcriptome of cells fractions described in <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002861#ppat-1002861-g001\" target=\"_blank\">Figure 1D</a>, at 1% FDR and 1.7 fold-change cut-off (represented by lines). The log10 of the p-value adjusted for multiple comparisons (Benjamini and Hochberg) is used for the Y axis and the log2 of the fold change is used for the X axis. Left columns: comparison between mock-infected versus uninfected bystander cell populations shows no statistically significant genes passing both thresholds at any time point or in aggregate analysis (labelled “all”). Center columns: comparison between mock-infected and virus-infected cells. Right columns: comparison between uninfected bystander and HIV-1-infected cells. B) Proportional Venn diagrams of above data, illustrating the overlap of DEGs at 24, 48 and 72 h for mock vs infected and bystander vs infected comparisons. C) Unsupervised hierarchical clustering (per sample and per gene) of differentially-expressed genes in the dataset. The similarity between mock-infected and uninfected bystander cells is obvious. Moreover, HIV-1-infected cell samples cluster together at the 24 h time point, but diverge on a per-sample basis at 48 and 72 h, illustrating that, although a common subset of T cells are infected early on, the precise context preferred by the virus can evolve differently over time on a per-sample basis.</p>", "links"=>[], "tags"=>["uninfected", "bystander", "hiv-1-infected"], "article_id"=>268359, "categories"=>["Genetics", "Infectious Diseases"], "users"=>["Michael Imbeault", "Katia Giguère", "Michel Ouellet", "Michel J. Tremblay"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002861.g002", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptomic_profile_of_mock_infected_uninfected_bystander_and_HIV_1_infected_cells_/268359", "title"=>"Transcriptomic profile of mock-infected, uninfected bystander and HIV-1-infected cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-02 02:19:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/598240"], "description"=>"<p>A) Co-citation analysis was performed with Bibliosphere and exported to Gephi. The data shown is from the HIV-1-infected versus uninfected bystander cells comparison at the 24 h time point. Pairs of genes with at least three abstracts co-citing the genes at the sentence level were considered as significant. Red genes are over-expressed in virus-infected cells while blue genes are under-expressed. The size of circles is inversely correlated to the corrected p-value obtained in the experiment (i.e. the bigger the circle, the smaller the p-value). The length of links between nodes is inversely correlated with the number of abstracts found in Pubmed for the two related genes. Gephi allows dynamic filtering and interaction with the data. Readers are encouraged to download Gephi (<a href=\"http://gephi.org\" target=\"_blank\">http://gephi.org</a>) and use the graph file (Dataset S2) to explore the dataset dynamically. Poorly studied genes having no significant relationship with other members are shown in the right portion of the graph. B) Hierarchical clustering of gene categories (Kruppel associated box – p = 3.7×10<sup>−3</sup> and GTPase regulator activity – p = 8.3×10<sup>−4</sup>) identified as being statistically over-represented among understudied genes.</p>", "links"=>[], "tags"=>["neglected"], "article_id"=>268738, "categories"=>["Genetics", "Infectious Diseases"], "users"=>["Michael Imbeault", "Katia Giguère", "Michel Ouellet", "Michel J. Tremblay"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002861.g004", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bibliography_analysis_and_focus_on_neglected_genes_/268738", "title"=>"Bibliography analysis and focus on neglected genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-02 02:25:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/313674", "https://ndownloader.figshare.com/files/313701", "https://ndownloader.figshare.com/files/313727", "https://ndownloader.figshare.com/files/313748", "https://ndownloader.figshare.com/files/313763", "https://ndownloader.figshare.com/files/313787", "https://ndownloader.figshare.com/files/313820", "https://ndownloader.figshare.com/files/313827"], "description"=>"<div><p>HIV-1 is extremely specialized since, even amongst CD4<sup>+</sup> T lymphocytes (its major natural reservoir in peripheral blood), the virus productively infects only a small proportion of cells under an activated state. As the percentage of HIV-1-infected cells is very low, most studies have so far failed to capture the precise transcriptomic profile at the whole-genome scale of cells highly susceptible to virus infection. Using Affymetrix Exon array technology and a reporter virus allowing the magnetic isolation of HIV-1-infected cells, we describe the host cell factors most favorable for virus establishment and replication along with an overview of virus-induced changes in host gene expression occurring exclusively in target cells productively infected with HIV-1. We also establish that within a population of activated CD4<sup>+</sup> T cells, HIV-1 has no detectable effect on the transcriptome of uninfected bystander cells at early time points following infection. The data gathered in this study provides unique insights into the biology of HIV-1-infected CD4<sup>+</sup> T cells and identifies genes thought to play a determinant role in the interplay between the virus and its host. Furthermore, it provides the first catalogue of alternative splicing events found in primary human CD4<sup>+</sup> T cells productively infected with HIV-1.</p> </div>", "links"=>[], "tags"=>["exon", "transcriptomic", "profiling", "hiv-1-infected", "cells", "reveals", "virus-induced", "genes", "viral", "replication"], "article_id"=>121870, "categories"=>["Cancer", "Genetics"], "users"=>["Michael Imbeault", "Katia Giguère", "Michel Ouellet", "Michel J. Tremblay"], "doi"=>["https://dx.doi.org/10.1371/journal.ppat.1002861.s001", "https://dx.doi.org/10.1371/journal.ppat.1002861.s002", "https://dx.doi.org/10.1371/journal.ppat.1002861.s003", "https://dx.doi.org/10.1371/journal.ppat.1002861.s004", "https://dx.doi.org/10.1371/journal.ppat.1002861.s005", "https://dx.doi.org/10.1371/journal.ppat.1002861.s006", "https://dx.doi.org/10.1371/journal.ppat.1002861.s007", "https://dx.doi.org/10.1371/journal.ppat.1002861.s008"], "stats"=>{"downloads"=>33, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Exon_Level_Transcriptomic_Profiling_of_HIV_1_Infected_CD4_T_Cells_Reveals_Virus_Induced_Genes_and_Host_Environment_Favorable_for_Viral_Replication/121870", "title"=>"Exon Level Transcriptomic Profiling of HIV-1-Infected CD4<sup>+</sup> T Cells Reveals Virus-Induced Genes and Host Environment Favorable for Viral Replication", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-08-02 00:31:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/598034"], "description"=>"<p>Unsupervised hierarchical clustering (per gene) of statistically over-represented Gene Ontology & KEGG pathway categories according to DAVID analysis. Since the first three categories (Immune system process – p = 1.9×10<sup>−17</sup>, Cytokine-cytokine receptor interaction – p 1×10<sup>−29</sup>, Regulation of leukocyte activation – p = 1.4×10<sup>−9</sup>) overlap significantly, they are presented as a single cluster. Positive and negative regulation of apoptosis (p = 5.8×10<sup>−7</sup> and 6×10<sup>−6</sup>, respectively) are both part of the Regulation of apoptosis category, but are here presented separately to better illustrate the absence of trend, in either direction. FOS/JUN (p = 2.3×10<sup>−5</sup>), the p53 pathway (p = 2.2×10<sup>−6</sup>) and Map kinase phosphatase (p = 2.3×10<sup>−5</sup>) are also identified as being over-represented and have implication for HIV-1 biology. All p-values mentioned above are corrected for multiple testing according to Bonferroni. The same color scale as <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002861#ppat-1002861-g002\" target=\"_blank\">Figure 2</a> applies.</p>", "links"=>[], "tags"=>["ontology", "overrepresentation", "genes", "differentially", "hiv-1-infected"], "article_id"=>268523, "categories"=>["Genetics", "Infectious Diseases"], "users"=>["Michael Imbeault", "Katia Giguère", "Michel Ouellet", "Michel J. Tremblay"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002861.g003", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gene_Ontology_overrepresentation_analysis_of_genes_differentially_expressed_in_HIV_1_infected_cells_/268523", "title"=>"Gene Ontology overrepresentation analysis of genes differentially expressed in HIV-1-infected cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-02 02:22:03"}
  • {"files"=>["https://ndownloader.figshare.com/files/598353"], "description"=>"<p>A) Proportional Venn diagram of the number of splicing events identified in virus-infected cells versus uninfected bystander cell populations at 24, 48 and 72 h post-infection. B) Confirmation by qRT-PCR of splicing events of interest. Pearson correlation coefficients indicate that the level of similarity between qRT-PCR quantification and microarrays is very high for all studied candidates. C) Overlay of the PECA-SI algorithm p-values and the splicing index (from aggregate comparisons) for each probeset on Affymetrix Exon arrays on a representation of selected genes in their genomic context. The exons that pass both filters (p-value and fold change – dotted line on their respective plots) are coloured red and those that pass only one filter colored orange. Red and blue arrow pairs represent primer sets used for qRT-PCR confirmation of splicing events (see above). PCR primers for confirmation of splicing events are also displayed (red – variable region between isoforms, blue – constant region). Top panel: PTPRC, better known as CD45, was selected as a target to validate our approach and filtering strategy, as CD45RO memory cells are more prone to be productively infected by HIV-1. Exon 4 is the one associated with the RA variant predominant in naïve cells. Middle panel: ITPR1, which plays a role in lymphocyte activation. Bottom panel: RUNX1 (AML1/EVI1), which is a master regulator of hematopoietic development important for T cell differentiation <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002861#ppat.1002861-Ichikawa1\" target=\"_blank\">[46]</a> and is known to have multiple isoforms arising from alternative promoter usage <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002861#ppat.1002861-Sroczynska1\" target=\"_blank\">[47]</a>.</p>", "links"=>[], "tags"=>["splicing", "events", "hiv-1-infected"], "article_id"=>268842, "categories"=>["Genetics", "Infectious Diseases"], "users"=>["Michael Imbeault", "Katia Giguère", "Michel Ouellet", "Michel J. Tremblay"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002861.g005", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alternative_splicing_events_are_found_in_HIV_1_infected_cells_/268842", "title"=>"Alternative splicing events are found in HIV-1-infected cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-02 02:27:22"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"42", "full-text"=>"46", "pdf"=>"23", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"43", "full-text"=>"44", "pdf"=>"36", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"37", "full-text"=>"34", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"33", "full-text"=>"29", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"25", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"10", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"39", "cited-by"=>"1", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[319, 556, 679, 785, 881, 970, 1062, 1149, 1236, 1323, 1402, 1474, 1545, 1617, 1681, 1754, 1822, 1892, 1963, 2031, 2099, 2165, 2233, 2299, 2359]}, {"subject_area"=>"/Biology and life sciences/Computational biology", "average_usage"=>[375, 629, 760, 889, 1000, 1110, 1203, 1298, 1399, 1492, 1603, 1699, 1774, 1855, 1918, 1998, 2062, 2152, 2227, 2312, 2378, 2461, 2528, 2600, 2664]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[333, 576, 707, 814, 908, 1004, 1104, 1197, 1280, 1370, 1449, 1531, 1603, 1673, 1742, 1817, 1886, 1954, 2025, 2098, 2171, 2234, 2304, 2365, 2431]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[339, 580, 709, 814, 907, 1003, 1095, 1190, 1286, 1375, 1466, 1546, 1622, 1694, 1765, 1830, 1902, 1978, 2054, 2122, 2194, 2269, 2330, 2388, 2460]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[321, 553, 683, 785, 880, 973, 1072, 1162, 1249, 1336, 1425, 1504, 1574, 1642, 1711, 1785, 1852, 1923, 1987, 2050, 2129, 2192, 2259, 2325, 2393]}]}
Loading … Spinner
There are currently no alerts