ADCC Develops Over Time during Persistent Infection with Live-Attenuated SIV and Is Associated with Complete Protection against SIVmac251 Challenge
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"ADCC Develops Over Time during Persistent Infection with Live-Attenuated SIV and Is Associated with Complete Protection against SIVmac251 Challenge", "type"=>"journal", "authors"=>[{"first_name"=>"Michael D.", "last_name"=>"Alpert", "scopus_author_id"=>"37030539600"}, {"first_name"=>"Jackson D.", "last_name"=>"Harvey", "scopus_author_id"=>"54397046000"}, {"first_name"=>"W. Anderson", "last_name"=>"Lauer", "scopus_author_id"=>"25028199500"}, {"first_name"=>"R. Keith", "last_name"=>"Reeves", "scopus_author_id"=>"18435214400"}, {"first_name"=>"Michael", "last_name"=>"Piatak", "scopus_author_id"=>"7003985150"}, {"first_name"=>"Angela", "last_name"=>"Carville", "scopus_author_id"=>"6602297148"}, {"first_name"=>"Keith G.", "last_name"=>"Mansfield", "scopus_author_id"=>"35461898800"}, {"first_name"=>"Jeffrey D.", "last_name"=>"Lifson", "scopus_author_id"=>"7006391588"}, {"first_name"=>"Wenjun", "last_name"=>"Li", "scopus_author_id"=>"55719039700"}, {"first_name"=>"Ronald C.", "last_name"=>"Desrosiers", "scopus_author_id"=>"7103155650"}, {"first_name"=>"R. Paul", "last_name"=>"Johnson", "scopus_author_id"=>"35227436000"}, {"first_name"=>"David T.", "last_name"=>"Evans", "scopus_author_id"=>"7601573391"}], "year"=>2012, "source"=>"PLoS Pathogens", "identifiers"=>{"scopus"=>"2-s2.0-84866150457", "doi"=>"10.1371/journal.ppat.1002890", "sgr"=>"84866150457", "isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "pmid"=>"22927823", "issn"=>"15537366", "pui"=>"365631459"}, "id"=>"b00c65b7-dfef-32dc-b05e-adacd1655af4", "abstract"=>"Live-attenuated strains of simian immunodeficiency virus (SIV) routinely confer apparent sterilizing immunity against pathogenic SIV challenge in rhesus macaques. Understanding the mechanisms of protection by live-attenuated SIV may provide important insights into the immune responses needed for protection against HIV-1. Here we investigated the development of antibodies that are functional against neutralization-resistant SIV challenge strains, and tested the hypothesis that these antibodies are associated with protection. In the absence of detectable neutralizing antibodies, Env-specific antibody-dependent cell-mediated cytotoxicity (ADCC) emerged by three weeks after inoculation with SIVΔnef, increased progressively over time, and was proportional to SIVΔnef replication. Persistent infection with SIVΔnef elicited significantly higher ADCC titers than immunization with a non-persistent SIV strain that is limited to a single cycle of infection. ADCC titers were higher against viruses matched to the vaccine strain in Env, but were measurable against viruses expressing heterologous Env proteins. In two separate experiments, which took advantage of either the strain-specificity or the time-dependent maturation of immunity to overcome complete protection against SIV(mac)251 challenge, measures of ADCC activity were higher among the SIVΔnef-inoculated macaques that remained uninfected than among those that became infected. These observations show that features of the antibody response elicited by SIVΔnef are consistent with hallmarks of protection by live-attenuated SIV, and reveal an association between Env-specific antibodies that direct ADCC and apparent sterilizing protection by SIVΔnef.", "link"=>"http://www.mendeley.com/research/adcc-develops-time-during-persistent-infection-liveattenuated-siv-associated-complete-protection-aga", "reader_count"=>34, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>3, "Student > Master"=>7, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>3, "Student > Master"=>7, "Other"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>19, "Medicine and Dentistry"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>8}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>8}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>19}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"United Kingdom"=>1, "France"=>1}, "group_count"=>0}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84866150457"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84866150457?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84866150457&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84866150457&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84866150457", "dc:identifier"=>"SCOPUS_ID:84866150457", "eid"=>"2-s2.0-84866150457", "dc:title"=>"ADCC Develops Over Time during Persistent Infection with Live-Attenuated SIV and Is Associated with Complete Protection against SIV<inf>mac</inf>251 Challenge", "dc:creator"=>"Alpert M.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"8", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-08-01", "prism:coverDisplayDate"=>"August 2012", "prism:doi"=>"10.1371/journal.ppat.1002890", "citedby-count"=>"110", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Harvard Medical School", "affiliation-city"=>"Boston", "affiliation-country"=>"United States"}], "pubmed-id"=>"22927823", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1002890", "source-id"=>"4000151809", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplospathogens%2Farticle%3Fid%3D10.1371%252Fjournal.ppat.1002890", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"62", "xml_views"=>"3", "html_views"=>"224"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"86", "xml_views"=>"1", "html_views"=>"326"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"74", "xml_views"=>"1", "html_views"=>"244"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"56", "xml_views"=>"1", "html_views"=>"152"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"97"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"223"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"63"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"108"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"4", "html_views"=>"55"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"4", "html_views"=>"70"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"65"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"3", "html_views"=>"76"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"344"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"353"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"151"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"153"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"35", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"40"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"4", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/588123"], "description"=>"<p>Plasma collected from 10 animals at 0, 3, 5, 7, 13 or 15, and 21 weeks after inoculation with SIV<sub>mac</sub>239Δ<i>nef</i> was evaluated for its capacity to neutralize SIV<sub>mac</sub>239 (A) and to direct ADCC against SIV<sub>mac</sub>239-infected cells (B). The loss of relative light units (RLU) indicates the loss of virus-infected cells during an 8-hour incubation in the presence of plasma and an NK cell line. Target cells infected by SHIV<sub>SF162P3</sub> served as a negative control for all ADCC assays (gray). Dashed lines indicate 50% activity. Neutralizing antibody titers were compared with 50% ADCC titers (C), and with AUC values for ADCC (D). An odds ratio (OR) for the probability of detecting neutralization per log<sub>10</sub> increase in 50% ADCC titer, or per 1 AUC unit increase in ADCC activity, was estimated by logistic regression.</p>", "links"=>[], "tags"=>["neutralizing", "antibody", "adcc", "titers", "macaques", "inoculated"], "article_id"=>258599, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Michael D. Alpert", "Jackson D. Harvey", "W. Anderson Lauer", "R. Keith Reeves", "Michael Piatak Jr.", "Angela Carville", "Keith G. Mansfield", "Jeffrey D. Lifson", "Wenjun Li", "Ronald C. Desrosiers", "R. Paul Johnson", "David T. Evans"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002890.g001", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Development_of_neutralizing_antibody_and_ADCC_titers_in_macaques_inoculated_with_SIV_mac_239_916_nef_/258599", "title"=>"Development of neutralizing antibody and ADCC titers in macaques inoculated with SIV<sub>mac</sub>239Δ<i>nef</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-23 02:23:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/309394", "https://ndownloader.figshare.com/files/309517", "https://ndownloader.figshare.com/files/309646", "https://ndownloader.figshare.com/files/309737"], "description"=>"<div><p>Live-attenuated strains of simian immunodeficiency virus (SIV) routinely confer apparent sterilizing immunity against pathogenic SIV challenge in rhesus macaques. Understanding the mechanisms of protection by live-attenuated SIV may provide important insights into the immune responses needed for protection against HIV-1. Here we investigated the development of antibodies that are functional against neutralization-resistant SIV challenge strains, and tested the hypothesis that these antibodies are associated with protection. In the absence of detectable neutralizing antibodies, Env-specific antibody-dependent cell-mediated cytotoxicity (ADCC) emerged by three weeks after inoculation with SIVΔ<em>nef</em>, increased progressively over time, and was proportional to SIVΔ<em>nef</em> replication. Persistent infection with SIVΔ<em>nef</em> elicited significantly higher ADCC titers than immunization with a non-persistent SIV strain that is limited to a single cycle of infection. ADCC titers were higher against viruses matched to the vaccine strain in Env, but were measurable against viruses expressing heterologous Env proteins. In two separate experiments, which took advantage of either the strain-specificity or the time-dependent maturation of immunity to overcome complete protection against SIV<sub>mac</sub>251 challenge, measures of ADCC activity were higher among the SIVΔ<em>nef</em>-inoculated macaques that remained uninfected than among those that became infected. These observations show that features of the antibody response elicited by SIVΔ<em>nef</em> are consistent with hallmarks of protection by live-attenuated SIV, and reveal an association between Env-specific antibodies that direct ADCC and apparent sterilizing protection by SIVΔ<em>nef</em>.</p> </div>", "links"=>[], "tags"=>["adcc", "develops", "persistent", "live-attenuated", "siv"], "article_id"=>121039, "categories"=>["Cancer", "Immunology"], "users"=>["Michael D. Alpert", "Jackson D. Harvey", "W. Anderson Lauer", "R. Keith Reeves", "Michael Piatak Jr.", "Angela Carville", "Keith G. Mansfield", "Jeffrey D. Lifson", "Wenjun Li", "Ronald C. Desrosiers", "R. Paul Johnson", "David T. Evans"], "doi"=>["https://dx.doi.org/10.1371/journal.ppat.1002890.s001", "https://dx.doi.org/10.1371/journal.ppat.1002890.s002", "https://dx.doi.org/10.1371/journal.ppat.1002890.s003", "https://dx.doi.org/10.1371/journal.ppat.1002890.s004"], "stats"=>{"downloads"=>3, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/ADCC_Develops_Over_Time_during_Persistent_Infection_with_Live_Attenuated_SIV_and_Is_Associated_with_Complete_Protection_against_SIV_mac_251_Challenge/121039", "title"=>"ADCC Develops Over Time during Persistent Infection with Live-Attenuated SIV and Is Associated with Complete Protection against SIV<sub>mac</sub>251 Challenge", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-08-23 00:17:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/588504"], "description"=>"<p>The extent of SIV<sub>mac</sub>239Δ<i>nef</i> or SIV<sub>mac</sub>239Δ<i>nef</i>/E543-3<i>env</i> replication was estimated from the area under the curve (AUC) of log<sub>10</sub>-transformed vaccine strain viral loads over weeks 0 through 21 or 22, and compared to ADCC activity at week 21 or 22. Vaccine strain viral load AUC values were correlated with 50% ADCC titers (A) against Env-matched (R<sub>S</sub> = 0.68, P<0.0001) and Env-mismatched (R<sub>S</sub> = 0.55, P = 0.006) SIV strains, and also with AUC values for ADCC activity (B) against Env-matched (R<sub>S</sub> = 0.64, P<0.0001) and Env-mismatched (R<sub>S</sub> = 0.42, P = 0.0421) SIV strains. Linear regression lines are drawn.</p>", "links"=>[], "tags"=>["replication", "adcc"], "article_id"=>258992, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Michael D. Alpert", "Jackson D. Harvey", "W. Anderson Lauer", "R. Keith Reeves", "Michael Piatak Jr.", "Angela Carville", "Keith G. Mansfield", "Jeffrey D. Lifson", "Wenjun Li", "Ronald C. Desrosiers", "R. Paul Johnson", "David T. Evans"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002890.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relationship_between_the_extent_of_vaccine_strain_replication_and_ADCC_activity_/258992", "title"=>"Relationship between the extent of vaccine strain replication and ADCC activity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-23 02:29:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/588582"], "description"=>"<p>Macaques were challenged with an intravenous dose of SIV<sub>mac</sub>251<sub>NE</sub> on week 46 after inoculation with SIV<sub>mac</sub>239Δ<i>nef</i> or SIV<sub>mac</sub>239Δ<i>nef</i>/E543-3<i>env</i>. Sera collected the day of challenge were evaluated for neutralization of SIV<sub>mac</sub>251<sub>NE</sub> (A) and ADCC against SIV<sub>mac</sub>251<sub>NE</sub>-infected cells (B). Solid black symbols indicate animals that became infected by SIV<sub>mac</sub>251<sub>NE</sub>. Dashed lines indicate 50% activity. Target cells infected with SHIV<sub>SF162P3</sub> served as a negative control for ADCC assays (gray). Differences in 50% ADCC titers were not significant (C). However, AUC values for ADCC were higher among the immunized animals that remained uninfected versus the immunized animals that became infected (2-tailed Mann-Whitney U test, P = 0.0091) (D). None of these macaques had the MHC class I alleles <i>Mamu</i>-<i>A*01</i>, <i>-B*08</i> or -<i>B*17</i> associated with reduced viral replication <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002890#ppat.1002890-Pal1\" target=\"_blank\">[67]</a>–<a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002890#ppat.1002890-Yant1\" target=\"_blank\">[69]</a>.</p>", "links"=>[], "tags"=>["adcc", "intravenous"], "article_id"=>259069, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Michael D. Alpert", "Jackson D. Harvey", "W. Anderson Lauer", "R. Keith Reeves", "Michael Piatak Jr.", "Angela Carville", "Keith G. Mansfield", "Jeffrey D. Lifson", "Wenjun Li", "Ronald C. Desrosiers", "R. Paul Johnson", "David T. Evans"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002890.g005", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Neutralization_and_ADCC_on_the_day_of_intravenous_challenge_with_SIV_mac_251_NE_/259069", "title"=>"Neutralization and ADCC on the day of intravenous challenge with SIV<sub>mac</sub>251<sub>NE</sub>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-23 02:31:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/588692"], "description"=>"<p>At 5, 20, or 40 weeks after inoculation with SIV<sub>mac</sub>239Δ<i>nef</i>, groups of 6 macaques each were challenged by high-dose vaginal inoculation with SIV<sub>mac</sub>251<sub>UCD</sub>. Serum collected the day of challenge was evaluated for neutralization of SIV<sub>mac</sub>251<sub>UCD</sub> (A–C) and ADCC against SIV<sub>mac</sub>251<sub>UCD</sub>-infected cells (D–F). Symbols appear in color for immunized macaques that remained uninfected by SIV<sub>mac</sub>251<sub>UCD</sub>, and in black for the immunized animals that became infected. Target cells infected with SHIV<sub>SF162P3</sub> served as a negative control for ADCC assays (gray). Dashed lines indicate 50% activity. The groups challenged on weeks 5, 20, and 40 were combined for statistical analysis (G–J). The SIV<sub>mac</sub>239Δ<i>nef</i>-immunized macaques remaining uninfected by SIV<sub>mac</sub>251<sub>UCD</sub> had higher 50% ADCC titers than those that became infected (2-tailed Mann-Whitney U test, P = 0.0487) (G). A similar but non-significant trend was observed in AUC values for ADCC (2-tailed Mann-Whitney U test, P = 0.0761) (H). Non-significant trends were in the direction of lower peak SIV<sub>mac</sub>251<sub>UCD</sub> viral loads for animals with higher 50% ADCC titers (R<sub>S</sub> = −0.4615, P = 0.1124) (I) and higher AUC measurements of ADCC activity (R<sub>S</sub> = −0.4560, P = 0.1173) (J). Linear regression lines are shown. The macaque with the lowest ADCC titers among those remaining uninfected was the only animal possessing the protective combination of MHC class I alleles <i>Mamu-A*01</i> and <i>-B*17 </i><a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002890#ppat.1002890-OConnor1\" target=\"_blank\">[70]</a>.</p>", "links"=>[], "tags"=>["adcc", "high-dose", "vaginal"], "article_id"=>259178, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Michael D. Alpert", "Jackson D. Harvey", "W. Anderson Lauer", "R. Keith Reeves", "Michael Piatak Jr.", "Angela Carville", "Keith G. Mansfield", "Jeffrey D. Lifson", "Wenjun Li", "Ronald C. Desrosiers", "R. Paul Johnson", "David T. Evans"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002890.g006", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Neutralization_and_ADCC_on_the_day_of_high_dose_vaginal_challenge_with_SIV_mac_251_UCD_/259178", "title"=>"Neutralization and ADCC on the day of high-dose vaginal challenge with SIV<sub>mac</sub>251<sub>UCD</sub>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-23 02:32:58"}
  • {"files"=>["https://ndownloader.figshare.com/files/588419"], "description"=>"<p>Sera drawn 0, 6, or 22 weeks after inoculation with SIV<sub>mac</sub>239Δ<i>nef</i> (A) or with the recombinant vaccine strain SIV<sub>mac</sub>239Δ<i>nef</i>/E543-3<i>env</i> (B) were tested for ADCC against target cells infected with SIV<sub>mac</sub>239 (black), SIV<sub>mac</sub>239/E543-3<i>env</i> (green), or SHIV<sub>SF162P3</sub> (gray). Dashed lines indicate 50% activity.</p>", "links"=>[], "tags"=>["viruses", "matched", "mismatched"], "article_id"=>258904, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Michael D. Alpert", "Jackson D. Harvey", "W. Anderson Lauer", "R. Keith Reeves", "Michael Piatak Jr.", "Angela Carville", "Keith G. Mansfield", "Jeffrey D. Lifson", "Wenjun Li", "Ronald C. Desrosiers", "R. Paul Johnson", "David T. Evans"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002890.g003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ADCC_against_viruses_matched_or_mismatched_to_the_vaccine_strain_in_Env_/258904", "title"=>"ADCC against viruses matched or mismatched to the vaccine strain in Env.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-23 02:28:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/588284"], "description"=>"<p>Plasma samples collected on weeks 2 and 12 after a series of inoculations with single-cycle SIV were titered for ADCC against SIV<sub>mac</sub>239-infected cells (A). Target cells infected with SHIV<sub>SF162P3</sub> served as a negative control (gray). Dashed lines indicate 50% activity. Geometric mean vaccine strain viral loads reflecting virus particles produced <i>in vivo</i> after inoculation with SIV<sub>mac</sub>239Δ<i>nef</i> or single-cycle SIV are shown (B). Animals in Group A were inoculated 3 times with single-cycle SIV that was <i>trans</i>-complemented with the vesicular stomatitis virus glycoprotein (VSV G), whereas the animals in Group B were inoculated 6 times with single-cycle SIV that was not <i>trans</i>-complemented <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002890#ppat.1002890-Jia1\" target=\"_blank\">[38]</a>. The 50% ADCC titers (C) and the AUC values for ADCC (D) elicited by SIV<sub>mac</sub>239Δ<i>nef</i> were significantly higher than those elicited by single-cycle SIV (2-tailed Mann-Whitney U tests, P = 0.0062 to P<0.0001). Binding titers measured by ELISA against SIV<sub>mac</sub>239 gp120 were correlated with 50% ADCC titers (E), and with AUC values for ADCC (F). Binding titers against SIV<sub>mac</sub>239 gp140 were also correlated with 50% ADCC titers (G), and with AUC values for ADCC (H).</p>", "links"=>[], "tags"=>["titers", "elicited", "single-cycle"], "article_id"=>258771, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Michael D. Alpert", "Jackson D. Harvey", "W. Anderson Lauer", "R. Keith Reeves", "Michael Piatak Jr.", "Angela Carville", "Keith G. Mansfield", "Jeffrey D. Lifson", "Wenjun Li", "Ronald C. Desrosiers", "R. Paul Johnson", "David T. Evans"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002890.g002", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ADCC_titers_elicited_by_SIV_mac_239_916_nef_versus_single_cycle_SIV_/258771", "title"=>"ADCC titers elicited by SIV<sub>mac</sub>239Δ<i>nef</i> versus single-cycle SIV.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-08-23 02:26:11"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"8"}
  • {"unique-ip"=>"53", "full-text"=>"52", "pdf"=>"24", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"35", "full-text"=>"39", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"24", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"19", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"26", "full-text"=>"33", "pdf"=>"13", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"30", "full-text"=>"32", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"8", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"19", "full-text"=>"25", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"33", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"19", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"22", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"5", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"39", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Immunology", "average_usage"=>[312, 555, 680, 785, 880, 965, 1057, 1142, 1230, 1312, 1394, 1471, 1547, 1613, 1681, 1743, 1805, 1871, 1941, 2010, 2071, 2128, 2202, 2266, 2323]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[339, 580, 709, 814, 907, 1003, 1095, 1190, 1286, 1375, 1466, 1546, 1622, 1694, 1765, 1830, 1902, 1978, 2054, 2122, 2194, 2269, 2330, 2388, 2460]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[331, 557, 677, 777, 868, 960, 1050, 1136, 1223, 1307, 1390, 1466, 1536, 1603, 1673, 1741, 1814, 1889, 1954, 2028, 2096, 2164, 2233, 2305, 2362]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[307, 536, 653, 753, 839, 926, 1017, 1103, 1189, 1268, 1348, 1425, 1492, 1557, 1620, 1686, 1759, 1825, 1891, 1950, 2014, 2079, 2141, 2200, 2255]}, {"subject_area"=>"/Medicine and health sciences/Immunology", "average_usage"=>[307, 545, 668, 765, 855, 943, 1029, 1113, 1202, 1285, 1361, 1439, 1506, 1571, 1636, 1706, 1772, 1837, 1901, 1969, 2031, 2096, 2160, 2228, 2287]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[321, 553, 683, 785, 880, 973, 1072, 1162, 1249, 1336, 1425, 1504, 1574, 1642, 1711, 1785, 1852, 1923, 1987, 2050, 2129, 2192, 2259, 2325, 2393]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[308, 535, 653, 754, 847, 930, 1020, 1103, 1188, 1268, 1351, 1420, 1487, 1550, 1614, 1679, 1757, 1830, 1898, 1961, 2024, 2086, 2150, 2216, 2266]}, {"subject_area"=>"/Medicine and health sciences/Public and occupational health", "average_usage"=>[327, 554, 673, 780, 874, 968, 1060, 1133, 1222, 1304, 1376, 1463, 1536, 1602, 1663, 1742, 1796, 1853, 1931, 1985, 2044, 2108, 2170, 2248, 2305]}]}

F1000Prime | Further Information

Loading … Spinner
There are currently no alerts