Plasmodium falciparum Gametocyte Development 1 (Pfgdv1) and Gametocytogenesis Early Gene Identification and Commitment to Sexual Development
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Plasmodium falciparum Gametocyte Development 1 (Pfgdv1) and Gametocytogenesis Early Gene Identification and Commitment to Sexual Development", "type"=>"journal", "authors"=>[{"first_name"=>"Saliha", "last_name"=>"Eksi", "scopus_author_id"=>"6506521243"}, {"first_name"=>"Belinda J.", "last_name"=>"Morahan", "scopus_author_id"=>"17342711200"}, {"first_name"=>"Yoseph", "last_name"=>"Haile", "scopus_author_id"=>"56563897000"}, {"first_name"=>"Tetsuya", "last_name"=>"Furuya", "scopus_author_id"=>"7202283994"}, {"first_name"=>"Hongying", "last_name"=>"Jiang", "scopus_author_id"=>"54990950300"}, {"first_name"=>"Omar", "last_name"=>"Ali", "scopus_author_id"=>"57196812480"}, {"first_name"=>"Huichun", "last_name"=>"Xu", "scopus_author_id"=>"57198605301"}, {"first_name"=>"Kirakorn", "last_name"=>"Kiattibutr", "scopus_author_id"=>"55440659600"}, {"first_name"=>"Amreena", "last_name"=>"Suri", "scopus_author_id"=>"24722099100"}, {"first_name"=>"Beata", "last_name"=>"Czesny", "scopus_author_id"=>"7801343966"}, {"first_name"=>"Adebowale", "last_name"=>"Adeyemo", "scopus_author_id"=>"56800223700"}, {"first_name"=>"Timothy G.", "last_name"=>"Myers", "scopus_author_id"=>"56686026500"}, {"first_name"=>"Jetsumon", "last_name"=>"Sattabongkot", "scopus_author_id"=>"7003649631"}, {"first_name"=>"Xin zhuan", "last_name"=>"Su", "scopus_author_id"=>"7402181904"}, {"first_name"=>"Kim C.", "last_name"=>"Williamson", "scopus_author_id"=>"7102039369"}], "year"=>2012, "source"=>"PLoS Pathogens", "identifiers"=>{"pmid"=>"23093935", "doi"=>"10.1371/journal.ppat.1002964", "sgr"=>"84868107919", "isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "scopus"=>"2-s2.0-84868107919", "issn"=>"15537366", "pui"=>"365953679"}, "id"=>"2490412f-a11e-3f35-9ccb-ef1c35cb0f2e", "abstract"=>"Malaria transmission requires the production of male and female gametocytes in the human host followed by fertilization and sporogonic development in the mosquito midgut. Although essential for the spread of malaria through the population, little is known about the initiation of gametocytogenesis in vitro or in vivo. Using a gametocyte-defective parasite line and genetic complementation, we show that Plasmodium falciparumgametocyte development 1 gene (Pfgdv1), encoding a peri-nuclear protein, is critical for early sexual differentiation. Transcriptional analysis of Pfgdv1 negative and positive parasite lines identified a set of gametocytogenesis early genes (Pfge) that were significantly down-regulated (>10 fold) in the absence of Pfgdv1 and expression was restored after Pfgdv1 complementation. Progressive accumulation of Pfge transcripts during successive rounds of asexual replication in synchronized cultures suggests that gametocytes are induced continuously during asexual growth. Comparison of Pfge gene transcriptional profiles in patient samples divided the genes into two groups differing in their expression in mature circulating gametocytes and providing candidates to evaluate gametocyte induction and maturation separately in vivo. The expression profile of one of the early gametocyte specific genes, Pfge1, correlated significantly with asexual parasitemia, which is consistent with the ongoing induction of gametocytogenesis during asexual growth observed in vitro and reinforces the need for sustained transmission-blocking strategies to eliminate malaria.", "link"=>"http://www.mendeley.com/research/plasmodium-falciparum-gametocyte-development-1-pfgdv1-gametocytogenesis-early-gene-identification-co", "reader_count"=>100, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>28, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>33, "Student > Postgraduate"=>7, "Student > Master"=>12, "Other"=>2, "Student > Bachelor"=>3, "Lecturer"=>3, "Professor"=>5}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Librarian"=>1, "Researcher"=>28, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>33, "Student > Postgraduate"=>7, "Student > Master"=>12, "Other"=>2, "Student > Bachelor"=>3, "Lecturer"=>3, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>18, "Agricultural and Biological Sciences"=>59, "Medicine and Dentistry"=>13, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>3, "Immunology and Microbiology"=>4, "Linguistics"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>13}, "Chemistry"=>{"Chemistry"=>3}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>59}, "Linguistics"=>{"Linguistics"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>18}, "Unspecified"=>{"Unspecified"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"United States"=>3, "United Kingdom"=>1, "Ghana"=>1}, "group_count"=>1}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84868107919"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84868107919?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84868107919&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84868107919&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84868107919", "dc:identifier"=>"SCOPUS_ID:84868107919", "eid"=>"2-s2.0-84868107919", "dc:title"=>"Plasmodium falciparum Gametocyte Development 1 (Pfgdv1) and Gametocytogenesis Early Gene Identification and Commitment to Sexual Development", "dc:creator"=>"Eksi S.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"8", "prism:issueIdentifier"=>"10", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-10-01", "prism:coverDisplayDate"=>"October 2012", "prism:doi"=>"10.1371/journal.ppat.1002964", "citedby-count"=>"59", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Loyola University of Chicago", "affiliation-city"=>"Chicago", "affiliation-country"=>"United States"}], "pubmed-id"=>"23093935", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1002964", "source-id"=>"4000151809", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplospathogens%2Farticle%3Fid%3D10.1371%252Fjournal.ppat.1002964", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"158", "xml_views"=>"19", "html_views"=>"811"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"97", "xml_views"=>"2", "html_views"=>"392"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"160"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"41", "xml_views"=>"4", "html_views"=>"317"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"220"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"236"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"178"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"19", "html_views"=>"154"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"8", "html_views"=>"159"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"160"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"49", "xml_views"=>"1", "html_views"=>"156"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"191"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"175"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"52", "xml_views"=>"0", "html_views"=>"203"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"210"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"23", "xml_views"=>"3", "html_views"=>"261"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"215"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"215"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"226"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"175"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"3", "html_views"=>"145"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"236"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"281"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"216"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"3", "html_views"=>"225"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"134"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"87"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"85"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"107"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"102"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"51", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"142"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"35", "xml_views"=>"1", "html_views"=>"132"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"105"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"84"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"81"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"79"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"2", "html_views"=>"71"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"32", "xml_views"=>"1", "html_views"=>"72"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"3", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"3", "html_views"=>"52"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"79", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"42", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"14"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/556672"], "description"=>"<p>The expression profiles of the relative abundance of indicated genes in relation to seryl tRNA synthetase at day 2 were compared to each other in the NAG treated (NAG) the Pearson correlation coefficient is indicated. Representative data shown is from one of 3 independent experiments.</p>", "links"=>[], "tags"=>["profiles", "nag-treated"], "article_id"=>227148, "categories"=>["Microbiology", "Developmental Biology", "Infectious Diseases"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002964.t001", "stats"=>{"downloads"=>2, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pearson_correlation_analysis_of_gene_expression_profiles_in_in_vitro_NAG_treated_cultures_/227148", "title"=>"Pearson correlation analysis of gene expression profiles in <i>in vitro</i>, NAG-treated cultures.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-10-18 01:59:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/556247"], "description"=>"<p>MACS purified late stage asexual parasite cultures were set up at 6% hematocrit and sorbitol synchronized 2 hours later to remove all but the newly invaded ring stage parasites. Parasitemia was monitored by daily Giemsa-stained smear. Ring (blue), schizont stage parasitemias (red), and stage II gametocytemia (green) are plotted (A & B). The relative abundance, (log2) of the indicated gene in relation to the expression level on day 2 was calculated using the 2<sup>−ΔΔ<i>C</i></sup><sub>T</sub> method <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002964#ppat.1002964-Schmittgen1\" target=\"_blank\">[43]</a> with seryl tRNA synthetase as the reference and plotted in C and D): <i>Pfaldolase</i> (blue), <i>Pfkahrp</i> (brown), <i>Pfmsp7-5</i> (purple), <i>Pfs16 (</i>green), <i>Pfgdv1</i> (bright red); while <i>Pfge1</i> (orange), <i>Pfge2</i> (pale pink), <i>Pfge3</i> (beige), <i>Pfg27</i> (light blue), <i>Pfge7</i> (dark pink), <i>Pfge8</i> (light green), <i>Pfs47</i>(turquoise) and <i>Pfs16</i> (green) are shown in (E and F). The 3 asexual cycles are indicated by numbers as well as gray dotted lines, and NAG treatment is indicated by the gray box. Representative data from one of three independent experiments is shown. The samples from the different time points were tested in triplicate and the average relative expression is plotted with the error bars representing the range.</p>", "links"=>[], "tags"=>["genes"], "article_id"=>226729, "categories"=>["Microbiology", "Developmental Biology", "Infectious Diseases"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002964.g004", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_profile_of_the_Pfge_genes_through_gametocytogenesis_/226729", "title"=>"Expression profile of the <i>Pfge</i> genes through gametocytogenesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-10-18 01:52:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/556141"], "description"=>"<p>Parasites transformed with GFP- or HA-tagged PfGDV1 were stained with DAPI (DNA stain) and the indicated anti-sera, and then examined by fluorescence microscopy (Zeiss Axiovert 200, 1000× magnification). Images are shown of the DAPI stain (DNA), GFP-tagged PfGDV1 epifluorescence (GDV1), and antibodies specific for HA (αHA), Pfs16 (αPfs16), PfGE3 (αPfGE3), PfMCM2 (αMCM2), and PfSir2 (αSir2). The corresponding merged and bright field (BF) images are included on the right. PfGDV1 expression is indicated with an arrow; locations of parasites in the BF image are indicated with a P for parasite or S for schizont. A) A schizont (S) (<i>Upper</i>) expressing GDV1 and a doubly infected erythrocyte (<i>Lower</i>) with one parasite (P1) expressing HA-tagged PfGDV1 (αHA) and another negative (P2) for anti-HA antibodies. B) Co-staining of parasites expressing GDV1 with early gametocytogenesis markers. A doubly infected erythrocyte (<i>Upper</i>) with one parasite (P1) positive for GDV1 and αPfs16 and the other (P2) negative for both. An erythrocyte (<i>Lower</i>) infected with a parasite (P) positive for GDV1 and αPfGE3. C) Co-localization of PfGDV1 with nuclear proteins. A doubly infected erythrocyte (<i>Upper</i>) with one parasite in the plane of the image (P1) and the other below (P2). Both P1 and P2 are positive for GDV1 and αMCM2. A schizont (S) (<i>Lower</i>) expressing GDV1 stained with αSir2.</p>", "links"=>[], "tags"=>["localization"], "article_id"=>226625, "categories"=>["Microbiology", "Developmental Biology", "Infectious Diseases"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002964.g003", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Subcellular_localization_of_PfGDV1_/226625", "title"=>"Subcellular localization of PfGDV1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-10-18 01:50:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/556721"], "description"=>"<p>The expression profiles of the relative abundance of indicated genes in relation to seryl tRNA synthetase at day 2 were compared to each other in the untreated cultures (No NAG) and the Pearson correlation coefficient is indicated. Representative data shown is from one of 3 independent experiments.</p>", "links"=>[], "tags"=>["profiles", "untreated"], "article_id"=>227201, "categories"=>["Microbiology", "Developmental Biology", "Infectious Diseases"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002964.t002", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pearson_correlation_analysis_of_gene_expression_profiles_in_in_vitro_untreated_cultures_/227201", "title"=>"Pearson correlation analysis of gene expression profiles in <i>in vitro</i> untreated cultures.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-10-18 02:00:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/555905"], "description"=>"<p>Mean ratios of microarray signals (mean±SEM) obtained from the 3D7.G+ and 3D7.G<sub>def</sub> clones at 1.4/0.9% parasitemia (dark gray bar) and at 5.2/5.5% parasitemia (light gray bar), respectively, for the 11 <i>Pfge</i> genes with a ratio of 3D7.G+/3D7.G<sub>def</sub>>10 are plotted in descending order. <i>Pfgdv1</i> (PFI1710w) had the fourth highest ratio and is indicated by an <sup>o</sup>. Previously described <i>Pfge</i> genes <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002964#ppat.1002964-Young1\" target=\"_blank\">[17]</a>, <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002964#ppat.1002964-Bruce2\" target=\"_blank\">[20]</a>–<a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002964#ppat.1002964-vanSchaijk1\" target=\"_blank\">[22]</a>, <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002964#ppat.1002964-Silvestrini3\" target=\"_blank\">[82]</a>, are denoted with an asterisk (*) and a vertical line indicates a gene represented by two oligonucleotides. <i>Pfge</i> number, PlasmoDB ID, and common name are listed on the right. The central panel is a summary of the characteristics of the <i>Pfgdv1</i>-dependent genes. The first row (L) indicates whether the gene is subtelomeric (<150 kb from the telomere) (gray square) or within a region of the chromosome that has synteny with other species (black square) <a href=\"http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002964#ppat.1002964-Kooij1\" target=\"_blank\">[83]</a>. The panel on the left indicates whether the gene encodes a secretory signal sequence (S, black square), PEXEL/HTS export domain (E, black square) or transmembrane domain (T, black square).</p>", "links"=>[], "tags"=>["clones", "identifies", "genes"], "article_id"=>226378, "categories"=>["Microbiology", "Developmental Biology", "Infectious Diseases"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002964.g001", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_analysis_of_the_3D7_G_and_3D7_G_def_clones_identifies_P_f_alciparum_g_ametocytogenesis_e_arly_genes_Pfge_/226378", "title"=>"Expression analysis of the 3D7.G+ and 3D7.G<sub>def</sub> clones identifies <i>P. falciparum</i>gametocytogenesis early genes (<i>Pfge</i>).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-10-18 01:46:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/556007"], "description"=>"<p>A) Schematic of chromosome 9 (1–1,541,723 bp) showing the segment deleted (arrows) in the 3D7.G<sub>def</sub> clone. For orientation, the putative centromere is indicated by an O and the 1,500,000 bp position is marked (1500 k). Colored boxes indicate the location of annotated genes: blue, genes transcribed toward the telomere; red, transcribed toward the centromere; horizontal stripes, <i>Pfgdv1;</i> diagonal stripes, <i>var</i> and <i>rifins</i>. The 70-mer oligonucleotide that identified the chromosome 9 deletion in 3D7.G<sub>def</sub> parasites is indicated by an asterisk (*). The numbered gray bars below the line indicate the positions of the eight PCR products used to map the chromosome 9 deletion. B) Amplification products from chromosome 9 using Indochina, 3D7.G<sub>def</sub>, FCR3.G<sub>def</sub>, or HB3.G<sub>def</sub> gDNA as a template. Peak gametocytemias attained in two independent experiments are indicated on the right of the ethidium bromide-stained agarose gel of the PCR products generated using the eight primer pairs (1–8). C) Southern blot of <i>Bsa</i>BI-digested gDNA from 3D7.G+ (+), 3D7.G<sub>def</sub> (d), 3D7.G<sub>def</sub> +<i>Pfgdv1</i> (a), 3D7.G<sub>def</sub> +HA.<i>Pfgdv1</i> (b) and the parental 3D7 parasites (WT). Digested gDNA was probed with <i>Pfgdv1</i> (bp 1423–1800) or <i>Pfg27</i> (bp 1–654). D–E) Gametocyte production in WT (black square), 3D7.G<sub>def</sub> (black triangle), complemented line a, 3D7.G<sub>def</sub> +<i>Pfgdv1</i> (Panel D, black inverted triangle) and line b, 3D7.G<sub>def</sub> +HA.<i>Pfgdv1</i> (Panel E, unfilled inverted triangle). Cultures set up at 0.1% asexual parasitemia on day 0 were followed for gametocyte production by Giemsa-stained smears for the next 16 days. Mean gametocytemia and standard deviation of two (D) or three (E) independent experiments are shown (<i>P</i><0.003 by linear regression analysis). F) Giemsa-stained smear of parasitized erythrocytes purified on a 16% Nycodenz cushion from day 16 gametocyte cultures of WT, 3D7.G<sub>def</sub> +HA.<i>Pfgdv1</i> (HA.<i>Pfgdv1</i>), and 3D7.G<sub>def</sub> (G<sub>def</sub>) lines. G) Northern blots of RNA harvested from WT 3D7 (w), 3D7.G<sub>def</sub> (d), 3D7.G<sub>def </sub><i>+Pfgdv1</i> (a), and 3D7.G<sub>def</sub> +HA.<i>Pfgdv1</i> (b) complemented lines were hybridized with probes corresponding to <i>Pfgdv1</i> (<i>gdv1</i>), <i>Pfge</i> genes (<i>ge1–3, 5–11</i>), and merozoite surface protein-1 (<i>msp1</i>) as an asexual parasite control. Autoradiographs are shown with the corresponding ethidium bromide-stained gel. H) Expression of gametocyte specific antigen Pfs48/45. Methanol-fixed WT, 3D7.G<sub>def</sub>+<i>Pfgdv1</i> (<i>a</i>) and 3D7.G<sub>def</sub>+HA.<i>Pfgdv1</i> (b) mature gametocyte cultures were incubated with Pfs48/45 mAb IIC5B10 (1∶250 dilution) and labeled secondary antibodies (1∶500 dilution). I) The average gametocytemia of 4 independent cultures of WT 3D7 (WT), WT 3D7 transformed with a <i>Pfgdv1</i> episomal expression plasmid (WT+HA.<i>Pfgdv1</i>) and the G<sub>def</sub> (G<sub>def</sub>) line is graphed. The error bars represent the SEM and a significant difference from WT and G<sub>def</sub> is indicated by an asterisk (p<0.05 ANOVA followed by Tukey multiple comparison test).</p>", "links"=>[], "tags"=>["gametocyte"], "article_id"=>226488, "categories"=>["Microbiology", "Developmental Biology", "Infectious Diseases"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002964.g002", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Identification_of_Plasmodium_falciparum_gametocyte_development_1_gene_Pfgdv1_/226488", "title"=>"Identification of <i>Plasmodium falciparum</i> gametocyte development 1 gene (<i>Pfgdv1</i>).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-10-18 01:48:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/556401"], "description"=>"<p>RNA from 20 gametocytemic patients was analyzed using a <i>P. falciparum</i> whole genome microarray. The color coded cluster analysis of the quantile normalized expression data for the <i>Pfge</i> genes with a G+/G<sub>def</sub> ratio>10 is shown with mature gametocyte specific genes <i>Pfs25</i> and <i>Pfs28</i> (Underlined in gray). The gene name and standardized intensity of the color code is indicated below and the patient cluster associated with high gametocytemia is indicated on the right with a vertical green bar. The gametocytemia (G'cyte) and asexual parasitemia (Asex) of the patient samples are represented both by color code and numerically on the right.</p>", "links"=>[], "tags"=>["vivo"], "article_id"=>226884, "categories"=>["Microbiology", "Developmental Biology", "Infectious Diseases"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002964.g005", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_vivo_Pfge_gene_expression_profiling_/226884", "title"=>"<i>In vivo Pfge</i> gene expression profiling.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-10-18 01:54:44"}
  • {"files"=>["https://ndownloader.figshare.com/files/296564", "https://ndownloader.figshare.com/files/296639", "https://ndownloader.figshare.com/files/296698", "https://ndownloader.figshare.com/files/296809", "https://ndownloader.figshare.com/files/296858"], "description"=>"<div><p>Malaria transmission requires the production of male and female gametocytes in the human host followed by fertilization and sporogonic development in the mosquito midgut. Although essential for the spread of malaria through the population, little is known about the initiation of gametocytogenesis <em>in vitro</em> or <em>in vivo</em>. Using a gametocyte-defective parasite line and genetic complementation, we show that <em><u>P</u>lasmodium <u>f</u>alciparum</em><u>g</u>ametocyte <u>d</u>evelopment 1 gene (<em>Pfgdv1)</em>, encoding a peri-nuclear protein, is critical for early sexual differentiation. Transcriptional analysis of <em>Pfgdv1</em> negative and positive parasite lines identified a set of <u>g</u>ametocytogenesis <u>e</u>arly genes (<em>Pfge</em>) that were significantly down-regulated (>10 fold) in the absence of <em>Pfgdv1</em> and expression was restored after <em>Pfgdv1</em> complementation. Progressive accumulation of <em>Pfge</em> transcripts during successive rounds of asexual replication in synchronized cultures suggests that gametocytes are induced continuously during asexual growth. Comparison of <em>Pfge</em> gene transcriptional profiles in patient samples divided the genes into two groups differing in their expression in mature circulating gametocytes and providing candidates to evaluate gametocyte induction and maturation separately <em>in vivo</em>. The expression profile of one of the early gametocyte specific genes, <em>Pfge1</em>, correlated significantly with asexual parasitemia, which is consistent with the ongoing induction of gametocytogenesis during asexual growth observed <em>in vitro</em> and reinforces the need for sustained transmission-blocking strategies to eliminate malaria.</p> </div>", "links"=>[], "tags"=>["gametocyte", "gametocytogenesis"], "article_id"=>118433, "categories"=>["Microbiology", "Cancer", "Developmental Biology"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>["https://dx.doi.org/10.1371/journal.ppat.1002964.s001", "https://dx.doi.org/10.1371/journal.ppat.1002964.s002", "https://dx.doi.org/10.1371/journal.ppat.1002964.s003", "https://dx.doi.org/10.1371/journal.ppat.1002964.s004", "https://dx.doi.org/10.1371/journal.ppat.1002964.s005"], "stats"=>{"downloads"=>22, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Plasmodium_falciparum_Gametocyte_Development_1_Pfgdv1_and_Gametocytogenesis_Early_Gene_Identification_and_Commitment_to_Sexual_Development/118433", "title"=>"<em>Plasmodium falciparum</em> Gametocyte Development 1 (<em>Pfgdv1</em>) and Gametocytogenesis Early Gene Identification and Commitment to Sexual Development", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-10-18 02:20:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/556565"], "description"=>"<p>During each round of the asexual cycle, a proportion of the schizonts (S<sub>c</sub>, light blue) produced are committed to producing merozoites (M<sub>c</sub>, light blue) that will differentiate into gametocytes (G<sub>c</sub>, G<sub>I–V</sub>) after invading a RBC. The expression profile for <i>Pfgdv1</i> is indicated by a line under the corresponding stage.</p>", "links"=>[], "tags"=>["Infectious diseases", "microbiology", "developmental biology"], "article_id"=>227039, "categories"=>["Microbiology", "Developmental Biology", "Infectious Diseases"], "users"=>["Saliha Eksi", "Belinda J. Morahan", "Yoseph Haile", "Tetsuya Furuya", "Hongying Jiang", "Omar Ali", "Huichun Xu", "Kirakorn Kiattibutr", "Amreena Suri", "Beata Czesny", "Adebowale Adeyemo", "Timothy G. Myers", "Jetsumon Sattabongkot", "Xin-zhuan Su", "Kim C. Williamson"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1002964.g006", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Model_for_continuous_gametocytogenesis_/227039", "title"=>"Model for continuous gametocytogenesis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-10-18 01:57:19"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"15", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"37", "full-text"=>"35", "pdf"=>"16", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"34", "full-text"=>"35", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"32", "full-text"=>"45", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"35", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"40", "full-text"=>"44", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"30", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"71", "full-text"=>"66", "pdf"=>"49", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"31", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"24", "full-text"=>"30", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"25", "full-text"=>"21", "pdf"=>"15", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"36", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"20", "pdf"=>"5", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"8", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"36", "full-text"=>"28", "pdf"=>"11", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"26", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"25", "full-text"=>"26", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"32", "full-text"=>"31", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"37", "full-text"=>"41", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"33", "full-text"=>"34", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"24", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"25", "full-text"=>"25", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"27", "full-text"=>"26", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"27", "full-text"=>"27", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"29", "full-text"=>"32", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"26", "full-text"=>"27", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"22", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"31", "full-text"=>"30", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"25", "full-text"=>"36", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"22", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"23", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"42", "full-text"=>"38", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"26", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"27", "full-text"=>"27", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"23", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"28", "full-text"=>"24", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"23", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"44", "full-text"=>"29", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"7", "cited-by"=>"1", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"24", "full-text"=>"24", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"38", "full-text"=>"254", "pdf"=>"15", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"24", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"32", "full-text"=>"26", "pdf"=>"14", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[313, 562, 690, 796, 893, 986, 1079, 1173, 1257, 1349, 1429, 1506, 1586, 1661, 1730, 1803, 1876, 1945, 2017, 2091, 2163, 2225, 2294, 2362, 2430]}, {"subject_area"=>"/Biology and life sciences/Parasitology", "average_usage"=>[352, 609, 728, 831, 918, 998, 1091, 1195, 1289, 1369, 1442, 1504, 1573, 1647, 1722, 1799, 1877, 1938, 2013, 2106, 2166, 2242, 2306, 2371, 2452]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[312, 547, 668, 769, 861, 953, 1046, 1135, 1224, 1307, 1388, 1464, 1536, 1606, 1676, 1744, 1812, 1882, 1954, 2020, 2089, 2155, 2218, 2282, 2344]}]}
Loading … Spinner
There are currently no alerts