An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation
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{"title"=>"An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation", "type"=>"journal", "authors"=>[{"first_name"=>"Stephanie", "last_name"=>"Traub", "scopus_author_id"=>"23483105900"}, {"first_name"=>"Alexandra", "last_name"=>"Nikonova", "scopus_author_id"=>"36639489200"}, {"first_name"=>"Alan", "last_name"=>"Carruthers", "scopus_author_id"=>"7102095992"}, {"first_name"=>"Rebecca", "last_name"=>"Dunmore", "scopus_author_id"=>"55587668400"}, {"first_name"=>"Katherine A.", "last_name"=>"Vousden", "scopus_author_id"=>"7006419357"}, {"first_name"=>"Leila", "last_name"=>"Gogsadze", "scopus_author_id"=>"55317122600"}, {"first_name"=>"Weidong", "last_name"=>"Hao", "scopus_author_id"=>"36765620200"}, {"first_name"=>"Qing", "last_name"=>"Zhu", "scopus_author_id"=>"37079825500"}, {"first_name"=>"Katie", "last_name"=>"Bernard", "scopus_author_id"=>"55513014100"}, {"first_name"=>"Jie", "last_name"=>"Zhu", "scopus_author_id"=>"8053729100"}, {"first_name"=>"Michael", "last_name"=>"Dymond", "scopus_author_id"=>"8300134900"}, {"first_name"=>"Gary R.", "last_name"=>"McLean", "scopus_author_id"=>"7102563460"}, {"first_name"=>"Ross P.", "last_name"=>"Walton", "scopus_author_id"=>"23491144800"}, {"first_name"=>"Nicholas", "last_name"=>"Glanville", "scopus_author_id"=>"23488491900"}, {"first_name"=>"Alison", "last_name"=>"Humbles", "scopus_author_id"=>"6602897655"}, {"first_name"=>"Musa", "last_name"=>"Khaitov", "scopus_author_id"=>"7801543864"}, {"first_name"=>"Ted", "last_name"=>"Wells", "scopus_author_id"=>"55844337500"}, {"first_name"=>"Roland", "last_name"=>"Kolbeck", "scopus_author_id"=>"7006021880"}, {"first_name"=>"Andrew J.", "last_name"=>"Leishman", "scopus_author_id"=>"6601992664"}, {"first_name"=>"Matthew A.", "last_name"=>"Sleeman", "scopus_author_id"=>"6603750601"}, {"first_name"=>"Nathan W.", "last_name"=>"Bartlett", "scopus_author_id"=>"7005608564"}, {"first_name"=>"Sebastian L.", "last_name"=>"Johnston", "scopus_author_id"=>"7401781716"}], "year"=>2013, "source"=>"PLoS Pathogens", "identifiers"=>{"sgr"=>"84883379483", "pmid"=>"23935498", "isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "pui"=>"369735854", "issn"=>"15537366", "scopus"=>"2-s2.0-84883379483", "doi"=>"10.1371/journal.ppat.1003520"}, "id"=>"49f299c1-dd88-3759-8af0-580878046b62", "abstract"=>"Human rhinoviruses (HRV) cause the majority of common colds and acute exacerbations of asthma and chronic obstructive pulmonary disease (COPD). Effective therapies are urgently needed, but no licensed treatments or vaccines currently exist. Of the 100 identified serotypes, ∼90% bind domain 1 of human intercellular adhesion molecule-1 (ICAM-1) as their cellular receptor, making this an attractive target for development of therapies; however, ICAM-1 domain 1 is also required for host defence and regulation of cell trafficking, principally via its major ligand LFA-1. Using a mouse anti-human ICAM-1 antibody (14C11) that specifically binds domain 1 of human ICAM-1, we show that 14C11 administered topically or systemically prevented entry of two major groups of rhinoviruses, HRV16 and HRV14, and reduced cellular inflammation, pro-inflammatory cytokine induction and virus load in vivo. 14C11 also reduced cellular inflammation and Th2 cytokine/chemokine production in a model of major group HRV-induced asthma exacerbation. Interestingly, 14C11 did not prevent cell adhesion via human ICAM-1/LFA-1 interactions in vitro, suggesting the epitope targeted by 14C11 was specific for viral entry. Thus a human ICAM-1 domain-1-specific antibody can prevent major group HRV entry and induction of airway inflammation in vivo.", "link"=>"http://www.mendeley.com/research/antihuman-icam1-antibody-inhibits-rhinovirusinduced-exacerbations-lung-inflammation", "reader_count"=>46, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>2, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>1, "Student > Master"=>5, "Other"=>3, "Student > Bachelor"=>3, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>5}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>2, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>14, "Student > Postgraduate"=>1, "Student > Master"=>5, "Other"=>3, "Student > Bachelor"=>3, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>5}, "reader_count_by_subject_area"=>{"Engineering"=>2, "Unspecified"=>4, "Biochemistry, Genetics and Molecular Biology"=>3, "Medicine and Dentistry"=>14, "Agricultural and Biological Sciences"=>16, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Immunology and Microbiology"=>6}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>14}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>6}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>4}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Netherlands"=>1, "United States"=>1, "Brazil"=>1, "Chile"=>1, "Germany"=>1}, "group_count"=>4}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1140176"], "description"=>"<p>Mice were dosed intranasally with 14C11 or isotype control 2 hours prior to intranasal infection with HRV16. (A)Total BAL cells, macrophages, lymphocytes and neutrophils were assessed with differentially stained cytospins day 2, 4 and 7 after infection. (B) CXCL1, CXCL10 and CXCL11 in the BAL were determined by MSD or quantitative ELISA. (C) HRV16 specific serum IgG1 and IgG2a on day 7 post-infection. Data are a pool of 2 experiments with n = 4 mice per group each. Data are expressed as mean (± SEM). Significance was assessed by Three-way analysis of variance (A and B) or by One-way ANOVA with Bonferroni's Multiple Comparison test as post-test (C). **p<0.01 and ***p<0.001 vs HRV16 infected transgenic negative mice; <sup>#</sup>p<0.05 and <sup>###</sup>p<0.001 vs HRV16 infected transgenic positive mice.</p>", "links"=>[], "tags"=>["immunology", "Immune system", "cytokines", "immunity", "Immune activation", "Immune defense", "Immunity to infections", "inflammation", "Innate immunity", "Immune cells", "microbiology", "Virology", "Viral transmission and infection", "coreceptors", "Viral attachment", "Viral entry", "Animal models of infection", "antivirals", "Host-pathogen interaction", "Model organisms", "Animal models", "mouse", "topically", "dosed", "14c11", "antibody-mediated", "inhibition", "hrv16"], "article_id"=>762229, "categories"=>["Biological Sciences"], "users"=>["Stephanie Traub", "Alexandra Nikonova", "Alan Carruthers", "Rebecca Dunmore", "Katherine A. Vousden", "Leila Gogsadze", "Weidong Hao", "Qing Zhu", "Katie Bernard", "Jie Zhu", "Michael Dymond", "Gary R. McLean", "Ross P. Walton", "Nicholas Glanville", "Alison Humbles", "Musa Khaitov", "Ted Wells", "Roland Kolbeck", "Andrew J. Leishman", "Matthew A. Sleeman", "Nathan W. Bartlett", "Sebastian L. Johnston"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003520.g006", "stats"=>{"downloads"=>2, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Time_course_of_topically_dosed_14C11_antibody_mediated_inhibition_of_major_group_HRV16_infection_/762229", "title"=>"Time course of topically dosed 14C11 antibody-mediated inhibition of major group HRV16 infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-08-01 03:54:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1140175"], "description"=>"<p>Mice were dosed intravenously with 14C11 24 hours prior to intranasal dosing with HRV1B, UV-inactivated HRV1B (UV) or HRV16 (n = 4 for tg− UV, tg− 1B, tg+ 16 iso and tg+ 16 14C11 groups; n = 6 for tg+ UV, tg+ 1B, tg+ 1B iso and tg+ 1B 14C11 groups). (A)Total BAL cells, neutrophils (on day 1) and lymphocytes (on day 4) were assessed with differentially stained cytospins. Data expressed as mean (± SEM). Significance was assessed by One-way ANOVA test with Bonferroni's Multiple Comparison test as post-test. ***p<0.001 vs UV-RV1B in transgenic negative mice; <sup>###</sup>p<0.001 vs UV-RV1B in transgenic positive mice; <sup>§</sup>p<0.05 and <sup>§§§</sup>p<0.001 vs isotype treated HRV16 infected transgenic positive mice. Data are representative of 2 independent experiments. (B) Groups of 7 mice were dosed intravenously with 14C11 24 hours prior to intranasal dosing with 1 µg LPS/mouse. Total BAL cells, lymphocytes and neutrophils were assessed with differentially stained cytospins day 1 after infection. Data are expressed as mean (± SEM). Significance was assessed by One-way ANOVA test with Bonferroni's Multiple Comparison test as post-test. *p<0.05, **p<0.01 and ***p<0.001 vs transgenic positive mice without treatment. Data are representative of 2 independent experiments.</p>", "links"=>[], "tags"=>["immunology", "Immune system", "cytokines", "immunity", "Immune activation", "Immune defense", "Immunity to infections", "inflammation", "Innate immunity", "Immune cells", "microbiology", "Virology", "Viral transmission and infection", "coreceptors", "Viral attachment", "Viral entry", "Animal models of infection", "antivirals", "Host-pathogen interaction", "Model organisms", "Animal models", "mouse", "inhibit", "induced", "mechanisms"], "article_id"=>762228, "categories"=>["Biological Sciences"], "users"=>["Stephanie Traub", "Alexandra Nikonova", "Alan Carruthers", "Rebecca Dunmore", "Katherine A. Vousden", "Leila Gogsadze", "Weidong Hao", "Qing Zhu", "Katie Bernard", "Jie Zhu", "Michael Dymond", "Gary R. McLean", "Ross P. Walton", "Nicholas Glanville", "Alison Humbles", "Musa Khaitov", "Ted Wells", "Roland Kolbeck", "Andrew J. Leishman", "Matthew A. Sleeman", "Nathan W. Bartlett", "Sebastian L. Johnston"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003520.g005", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_14C11_does_not_inhibit_inflammation_induced_via_mechanisms_independent_of_human_ICAM_1_/762228", "title"=>"14C11 does not inhibit inflammation induced via mechanisms independent of human ICAM-1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-08-01 03:54:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1140178", "https://ndownloader.figshare.com/files/1140179", "https://ndownloader.figshare.com/files/1140180", "https://ndownloader.figshare.com/files/1140181", "https://ndownloader.figshare.com/files/1140182"], "description"=>"<div><p>Human rhinoviruses (HRV) cause the majority of common colds and acute exacerbations of asthma and chronic obstructive pulmonary disease (COPD). Effective therapies are urgently needed, but no licensed treatments or vaccines currently exist. Of the 100 identified serotypes, ∼90% bind domain 1 of human intercellular adhesion molecule-1 (ICAM-1) as their cellular receptor, making this an attractive target for development of therapies; however, ICAM-1 domain 1 is also required for host defence and regulation of cell trafficking, principally via its major ligand LFA-1. Using a mouse anti-human ICAM-1 antibody (14C11) that specifically binds domain 1 of human ICAM-1, we show that 14C11 administered topically or systemically prevented entry of two major groups of rhinoviruses, HRV16 and HRV14, and reduced cellular inflammation, pro-inflammatory cytokine induction and virus load <i>in vivo</i>. 14C11 also reduced cellular inflammation and Th2 cytokine/chemokine production in a model of major group HRV-induced asthma exacerbation. Interestingly, 14C11 did not prevent cell adhesion via human ICAM-1/LFA-1 interactions <i>in vitro,</i> suggesting the epitope targeted by 14C11 was specific for viral entry. Thus a human ICAM-1 domain-1-specific antibody can prevent major group HRV entry and induction of airway inflammation <i>in vivo</i>.</p></div>", "links"=>[], "tags"=>["immunology", "Immune system", "cytokines", "immunity", "Immune activation", "Immune defense", "Immunity to infections", "inflammation", "Innate immunity", "Immune cells", "microbiology", "Virology", "Viral transmission and infection", "coreceptors", "Viral attachment", "Viral entry", "Animal models of infection", "antivirals", "Host-pathogen interaction", "Model organisms", "Animal models", "mouse", "anti-human", "icam-1", "antibody", "inhibits", "rhinovirus-induced", "exacerbations"], "article_id"=>762231, "categories"=>["Biological Sciences"], "users"=>["Stephanie Traub", "Alexandra Nikonova", "Alan Carruthers", "Rebecca Dunmore", "Katherine A. Vousden", "Leila Gogsadze", "Weidong Hao", "Qing Zhu", "Katie Bernard", "Jie Zhu", "Michael Dymond", "Gary R. McLean", "Ross P. Walton", "Nicholas Glanville", "Alison Humbles", "Musa Khaitov", "Ted Wells", "Roland Kolbeck", "Andrew J. Leishman", "Matthew A. Sleeman", "Nathan W. Bartlett", "Sebastian L. Johnston"], "doi"=>["https://dx.doi.org/10.1371/journal.ppat.1003520.s001", "https://dx.doi.org/10.1371/journal.ppat.1003520.s002", "https://dx.doi.org/10.1371/journal.ppat.1003520.s003", "https://dx.doi.org/10.1371/journal.ppat.1003520.s004", "https://dx.doi.org/10.1371/journal.ppat.1003520.s005"], "stats"=>{"downloads"=>10, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_An_Anti_Human_ICAM_1_Antibody_Inhibits_Rhinovirus_Induced_Exacerbations_of_Lung_Inflammation_/762231", "title"=>"An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-08-01 03:54:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1140161"], "description"=>"<p>Ohio HeLa cells were pre-incubated with serial dilutions of 14C11 or isotype control and infected with (A) HRV16, (B) HRV14 and (C) minor group HRV25. The antiviral effect of 14C11 was determined by CPE reduction assay and expressed as % of control. (D) IC<sub>50</sub>s for 14C11 were determined for major HRVs by CPE assay as indicated in. Experiment (A), (B) and (C) were performed in sextuplicates and (D) is a pool of two independent experiments. Data are expressed as mean (± SEM).</p>", "links"=>[], "tags"=>["immunology", "Immune system", "cytokines", "immunity", "Immune activation", "Immune defense", "Immunity to infections", "inflammation", "Innate immunity", "Immune cells", "microbiology", "Virology", "Viral transmission and infection", "coreceptors", "Viral attachment", "Viral entry", "Animal models of infection", "antivirals", "Host-pathogen interaction", "Model organisms", "Animal models", "mouse", "inhibits", "hrv", "replication"], "article_id"=>762213, "categories"=>["Biological Sciences"], "users"=>["Stephanie Traub", "Alexandra Nikonova", "Alan Carruthers", "Rebecca Dunmore", "Katherine A. Vousden", "Leila Gogsadze", "Weidong Hao", "Qing Zhu", "Katie Bernard", "Jie Zhu", "Michael Dymond", "Gary R. McLean", "Ross P. Walton", "Nicholas Glanville", "Alison Humbles", "Musa Khaitov", "Ted Wells", "Roland Kolbeck", "Andrew J. Leishman", "Matthew A. Sleeman", "Nathan W. Bartlett", "Sebastian L. Johnston"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003520.g002", "stats"=>{"downloads"=>6, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_14C11_inhibits_major_group_HRV_replication_in_vitro_/762213", "title"=>"14C11 inhibits major group HRV replication <i>in vitro</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-08-01 03:54:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1140177"], "description"=>"<p>Groups of 8 transgenic positive mice were OVA sensitised on day −13 and re-challenged either with PBS or OVA on day −2 and day −1. On day 0 mice were dosed intranasally with 14C11 or isotype control 2 hours prior to intranasal challenge with PBS or OVA and infected HRV16 (RV-PBS and RV-OVA) or UV-inactivated HRV16 (UV-PBS or UV-OVA). (A) Total BAL cells, neutrophil and macrophage numbers were assessed on day 2 after infection and (B) lymphocyte and eosinophil numbers on day 6 after infection. (C) Airway hyper-responsiveness was determined on day 1 after infection. The levels of cytokines and chemokines (D) IL-4, IL-5, IL-13, IL-6 and CXCL1 in BAL and CXCL11 in lung homogenate 2 days after infection. (E) Total IgE in serum and levels of MUC5AC and MUC5B protein in BAL on day 6 after infection. Significance was assessed by One-way ANOVA test with Bonferroni's Multiple Comparison test as post-test. *p<0.05, **p<0.01 and ***p<0.001 vs transgenic positive mice pretreated with isotype control and challenged with RV-OVA. Data are representative of 3 independent experiments.</p>", "links"=>[], "tags"=>["immunology", "Immune system", "cytokines", "immunity", "Immune activation", "Immune defense", "Immunity to infections", "inflammation", "Innate immunity", "Immune cells", "microbiology", "Virology", "Viral transmission and infection", "coreceptors", "Viral attachment", "Viral entry", "Animal models of infection", "antivirals", "Host-pathogen interaction", "Model organisms", "Animal models", "mouse", "topically", "dosed", "14c11", "antibody", "hrv16", "induced", "exacerbation", "allergic", "airway"], "article_id"=>762230, "categories"=>["Biological Sciences"], "users"=>["Stephanie Traub", "Alexandra Nikonova", "Alan Carruthers", "Rebecca Dunmore", "Katherine A. Vousden", "Leila Gogsadze", "Weidong Hao", "Qing Zhu", "Katie Bernard", "Jie Zhu", "Michael Dymond", "Gary R. McLean", "Ross P. Walton", "Nicholas Glanville", "Alison Humbles", "Musa Khaitov", "Ted Wells", "Roland Kolbeck", "Andrew J. Leishman", "Matthew A. Sleeman", "Nathan W. Bartlett", "Sebastian L. Johnston"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003520.g007", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_topically_dosed_14C11_antibody_on_HRV16_induced_exacerbation_of_allergic_airway_inflammation_in_vivo_/762230", "title"=>"Effect of topically dosed 14C11 antibody on HRV16 induced exacerbation of allergic airway inflammation in vivo.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-08-01 03:54:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1140174"], "description"=>"<p>Mice were dosed intravenously with 14C11 24 hours prior to intranasal infection with HRV16 (n = 9 for tg− group; n = 6 for tg+ groups). (A) Total BAL cells, amacrophageslymphocytes and neutrophils were assessed by cytospin 2 days after infection. (B) The chemokines CXCL1, CXCL11 and CXCL10 in BAL were determined by MSD or quantitative ELISA 2 days after infection. Data are expressed as mean (± SEM). Significance was assessed by One-way ANOVA test with Bonferroni's Multiple Comparison test as post-test. **p<0.01 and ***p<0.001 vs HRV16 infected transgenic negative mice; <sup>#</sup>p<0.05, <sup>##</sup>p<0.01 and <sup>###</sup>p<0.001 vs HRV16 infected transgenic positive mice; Data are representative of 3 independent experiments.</p>", "links"=>[], "tags"=>["immunology", "Immune system", "cytokines", "immunity", "Immune activation", "Immune defense", "Immunity to infections", "inflammation", "Innate immunity", "Immune cells", "microbiology", "Virology", "Viral transmission and infection", "coreceptors", "Viral attachment", "Viral entry", "Animal models of infection", "antivirals", "Host-pathogen interaction", "Model organisms", "Animal models", "mouse", "systemically", "dosed", "14c11", "antibody", "hrv16"], "article_id"=>762227, "categories"=>["Biological Sciences"], "users"=>["Stephanie Traub", "Alexandra Nikonova", "Alan Carruthers", "Rebecca Dunmore", "Katherine A. Vousden", "Leila Gogsadze", "Weidong Hao", "Qing Zhu", "Katie Bernard", "Jie Zhu", "Michael Dymond", "Gary R. McLean", "Ross P. Walton", "Nicholas Glanville", "Alison Humbles", "Musa Khaitov", "Ted Wells", "Roland Kolbeck", "Andrew J. Leishman", "Matthew A. Sleeman", "Nathan W. Bartlett", "Sebastian L. Johnston"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003520.g004", "stats"=>{"downloads"=>4, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_systemically_dosed_14C11_antibody_on_HRV16_infection_in_vivo_/762227", "title"=>"Effect of systemically dosed 14C11 antibody on HRV16 infection in vivo.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-08-01 03:54:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1140158"], "description"=>"<p>(A)14C11 and (B) 84H10 antibody were tested for binding of antigens (human ICAM-1 (Hu ICAM-1), mouse ICAM-1 (Mu ICAM-1) and a recombinant protein consisting of human ICAM-1 D1 and mouse ICAM-1 D2-5 (Hu1 Mu2-5)). Jurkat E6.1 cells were labelled with Calcein-AM dye. Serial dilutions of the antibodies 14C11, 84H10 and isotype control were added in an ICAM-1-Fc coated plate. (C) PMA and labelled Jurkat E6.1 cells were added into the plate. Binding to LFA-1 was determined by fluorescein detection. Data were analysed by normalising values to no PMA (0% signal) and PMA no antibody (100% signal) controls. Data are a representative of three experiments for (A) and (B) and four to seven experiments for (C). Data are expressed as mean (± SEM).</p>", "links"=>[], "tags"=>["immunology", "Immune system", "cytokines", "immunity", "Immune activation", "Immune defense", "Immunity to infections", "inflammation", "Innate immunity", "Immune cells", "microbiology", "Virology", "Viral transmission and infection", "coreceptors", "Viral attachment", "Viral entry", "Animal models of infection", "antivirals", "Host-pathogen interaction", "Model organisms", "Animal models", "mouse", "binds", "icam-1", "inhibit"], "article_id"=>762212, "categories"=>["Biological Sciences"], "users"=>["Stephanie Traub", "Alexandra Nikonova", "Alan Carruthers", "Rebecca Dunmore", "Katherine A. Vousden", "Leila Gogsadze", "Weidong Hao", "Qing Zhu", "Katie Bernard", "Jie Zhu", "Michael Dymond", "Gary R. McLean", "Ross P. Walton", "Nicholas Glanville", "Alison Humbles", "Musa Khaitov", "Ted Wells", "Roland Kolbeck", "Andrew J. Leishman", "Matthew A. Sleeman", "Nathan W. Bartlett", "Sebastian L. Johnston"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003520.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_14C11_binds_in_domain_1_of_human_ICAM_1_does_not_bind_to_mouse_ICAM_1_and_does_not_inhibit_human_ICAM_1_LFA_1_interaction_/762212", "title"=>"14C11 binds in domain 1 of human ICAM-1, does not bind to mouse ICAM-1 and does not inhibit human ICAM-1/LFA-1 interaction.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-08-01 03:54:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1140173"], "description"=>"<p>Groups of 7 mice were dosed intranasally with 14C11 or isotype control 2 hours prior to intranasal infection with HRV16. (A) Total BAL cells, macrophages, lymphocytes and neutrophils were assessed with differentially cytospin counts day 2 after infection. (B) Levels of proinflammatory cytokines and chemokines IL-1β, IL-6, CXCL1, IFNλ2/3, CXCL11 and CXCL10 in cell-free BAL were determined by MSD or quantitative ELISA 2 days after infection. (C) Proinflammatory cytokines and chemokines IL-1β, IL-6 and CXCL1 in lung homogenate were assessed with MSD 2 days after infection. (D) Groups of 3–5 mice were dosed intranasally with 14C11 or isotype control 2 hours prior to intranasal infection with HRV16 and vRNA in lung tissue was assessed by qPCR at the timepoints indicated. (E) Inflammatory cells in the lungs prior to infection (uninfected) and at 12 hours post-infection (12 p.i.) of huICAM negative (tg−) and huICAM-expressing (tg+) mice without antibody treatment, or for tg+ mice following isotype control antibody (tg+ iso) or 14C11 antibody treatment (tg+ 14C11) assessed by H&E staining Arrows indicate peribronchial cellular inflammation. Data are expressed as mean (± SEM). Significance was assessed by One-way ANOVA test with Bonferroni's Multiple Comparison test as post-test. ***p<0.001 and **p<0.01 vs HRV16 infected transgenic negative mice; <sup>#</sup>p<0.05, <sup>##</sup>p<0.01 and <sup>###</sup>p<0.001 vs HRV16 infected transgenic positive mice. Data are representative of 3 independent experiments each.</p>", "links"=>[], "tags"=>["immunology", "Immune system", "cytokines", "immunity", "Immune activation", "Immune defense", "Immunity to infections", "inflammation", "Innate immunity", "Immune cells", "microbiology", "Virology", "Viral transmission and infection", "coreceptors", "Viral attachment", "Viral entry", "Animal models of infection", "antivirals", "Host-pathogen interaction", "Model organisms", "Animal models", "mouse", "antibody", "inhibits", "hrv16"], "article_id"=>762226, "categories"=>["Biological Sciences"], "users"=>["Stephanie Traub", "Alexandra Nikonova", "Alan Carruthers", "Rebecca Dunmore", "Katherine A. Vousden", "Leila Gogsadze", "Weidong Hao", "Qing Zhu", "Katie Bernard", "Jie Zhu", "Michael Dymond", "Gary R. McLean", "Ross P. Walton", "Nicholas Glanville", "Alison Humbles", "Musa Khaitov", "Ted Wells", "Roland Kolbeck", "Andrew J. Leishman", "Matthew A. Sleeman", "Nathan W. Bartlett", "Sebastian L. Johnston"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003520.g003", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_14C11_antibody_inhibits_major_group_HRV16_infection_in_vivo_/762226", "title"=>"14C11 antibody inhibits major group HRV16 infection <i>in vivo</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-08-01 03:54:08"}

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