Static Clathrin Assemblies at the Peripheral Vacuole—Plasma Membrane Interface of the Parasitic Protozoan Giardia lamblia
Publication Date
July 20, 2016
Journal
PLOS Pathogens
Authors
Jon Paulin Zumthor, Lenka Cernikova, Samuel Rout, Andres Kaech, et al
Volume
12
Issue
7
Pages
e1005756
DOI
https://dx.plos.org/10.1371/journal.ppat.1005756
Publisher URL
http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1005756
Web of Science
000383366400037
Scopus
84980519603
Mendeley
http://www.mendeley.com/research/static-clathrin-assemblies-peripheral-vacuoleplasma-membrane-interface-parasitic-protozoan-giardia-l
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Mendeley | Further Information

{"title"=>"Static Clathrin Assemblies at the Peripheral Vacuole—Plasma Membrane Interface of the Parasitic Protozoan Giardia lamblia", "type"=>"journal", "authors"=>[{"first_name"=>"Jon Paulin", "last_name"=>"Zumthor", "scopus_author_id"=>"57190752603"}, {"first_name"=>"Lenka", "last_name"=>"Cernikova", "scopus_author_id"=>"57194738789"}, {"first_name"=>"Samuel", "last_name"=>"Rout", "scopus_author_id"=>"35488771400"}, {"first_name"=>"Andres", "last_name"=>"Kaech", "scopus_author_id"=>"36466644100"}, {"first_name"=>"Carmen", "last_name"=>"Faso", "scopus_author_id"=>"24076032900"}, {"first_name"=>"Adrian B.", "last_name"=>"Hehl", "scopus_author_id"=>"6603965734"}], "year"=>2016, "source"=>"PLoS Pathogens", "identifiers"=>{"issn"=>"15537374", "scopus"=>"2-s2.0-84982953409", "sgr"=>"84982953409", "pui"=>"611582031", "isbn"=>"1553-7374 (Electronic)\r1553-7366 (Linking)", "pmid"=>"27438602", "doi"=>"10.1371/journal.ppat.1005756"}, "id"=>"5a560538-317e-3861-8e56-7c4722a68f39", "abstract"=>"Giardia lamblia is a parasitic protozoan that infects a wide range of vertebrate hosts including humans. Trophozoites are non-invasive but associate tightly with the enterocyte surface of the small intestine. This narrow ecological specialization entailed extensive morphological and functional adaptations during host-parasite co-evolution, including a distinctly polarized array of endocytic organelles termed peripheral vacuoles (PVs), which are confined to the dorsal cortical region exposed to the gut lumen and are in close proximity to the plasma membrane (PM). Here, we investigated the molecular consequences of these adaptations on the Giardia endocytic machinery and membrane coat complexes. Despite the absence of canonical clathrin coated vesicles in electron microscopy, Giardia possesses conserved PV-associated clathrin heavy chain (GlCHC), dynamin-related protein (GlDRP), and assembly polypeptide complex 2 (AP2) subunits, suggesting a novel function for GlCHC and its adaptors. We found that, in contrast to GFP-tagged AP2 subunits and DRP, CHC::GFP reporters have no detectable turnover in living cells, indicating fundamental differences in recruitment to the membrane and disassembly compared to previously characterized clathrin coats. Histochemical localization in electron tomography showed that these long-lived GlCHC assemblies localized at distinctive approximations between the plasma and PV membrane. A detailed protein interactome of GlCHC revealed all of the conserved factors in addition to novel or highly diverged proteins, including a putative clathrin light chain and lipid-binding proteins. Taken together, our data provide strong evidence for giardial CHC as a component of highly stable assemblies at PV-PM junctions that likely have a central role in organizing continuities between the PM and PV membranes for controlled sampling of the fluid environment. This suggests a novel function for CHC in Giardia and the extent of molecular remodeling of endocytosis in this species.", "link"=>"http://www.mendeley.com/research/static-clathrin-assemblies-peripheral-vacuoleplasma-membrane-interface-parasitic-protozoan-giardia-l", "reader_count"=>24, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Researcher"=>2, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>8, "Student > Master"=>2, "Student > Bachelor"=>4, "Lecturer > Senior Lecturer"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Researcher"=>2, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>8, "Student > Master"=>2, "Student > Bachelor"=>4, "Lecturer > Senior Lecturer"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Biochemistry, Genetics and Molecular Biology"=>9, "Agricultural and Biological Sciences"=>9, "Neuroscience"=>1, "Veterinary Science and Veterinary Medicine"=>1}, "reader_count_by_subdiscipline"=>{"Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>9}, "Unspecified"=>{"Unspecified"=>4}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Switzerland"=>1}, "group_count"=>0}

Scopus | Further Information

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