Two Different Virulence-Related Regulatory Pathways in Borrelia burgdorferi Are Directly Affected by Osmotic Fluxes in the Blood Meal of Feeding Ixodes Ticks
Publication Date
August 15, 2016
Journal
PLOS Pathogens
Authors
Sébastien Bontemps Gallo, Kevin Lawrence & Frank C. Gherardini
Volume
12
Issue
8
Pages
e1005791
DOI
https://dx.plos.org/10.1371/journal.ppat.1005791
Publisher URL
http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1005791
Web of Science
000383376000029
Scopus
84984833765
Mendeley
http://www.mendeley.com/research/two-different-virulencerelated-regulatory-pathways-borrelia-burgdorferi-directly-affected-osmotic-fl
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Mendeley | Further Information

{"title"=>"Two Different Virulence-Related Regulatory Pathways in Borrelia burgdorferi Are Directly Affected by Osmotic Fluxes in the Blood Meal of Feeding Ixodes Ticks", "type"=>"journal", "authors"=>[{"first_name"=>"Sébastien", "last_name"=>"Bontemps-Gallo", "scopus_author_id"=>"55521775500"}, {"first_name"=>"Kevin", "last_name"=>"Lawrence", "scopus_author_id"=>"16417566400"}, {"first_name"=>"Frank C.", "last_name"=>"Gherardini", "scopus_author_id"=>"6603671831"}], "year"=>2016, "source"=>"PLoS Pathogens", "identifiers"=>{"pui"=>"611987978", "sgr"=>"84984833765", "pmid"=>"27525653", "scopus"=>"2-s2.0-84984833765", "doi"=>"10.1371/journal.ppat.1005791", "issn"=>"15537374"}, "id"=>"1ebfbf2c-edb8-34cf-8753-28d0088f1c7c", "abstract"=>"Lyme disease, caused by Borrelia burgdorferi, is a vector-borne illness that requires the bacteria to adapt to distinctly different environments in its tick vector and various mammalian hosts. Effective colonization (acquisition phase) of a tick requires the bacteria to adapt to tick midgut physiology. Successful transmission (transmission phase) to a mammal requires the bacteria to sense and respond to the midgut environmental cues and up-regulate key virulence factors before transmission to a new host. Data presented here suggest that one environmental signal that appears to affect both phases of the infective cycle is osmolarity. While constant in the blood, interstitial fluid and tissue of a mammalian host (300 mOsm), osmolarity fluctuates in the midgut of feeding Ixodes scapularis. Measured osmolarity of the blood meal isolated from the midgut of a feeding tick fluctuates from an initial osmolarity of 600 mOsm to blood-like osmolarity of 300 mOsm. After feeding, the midgut osmolarity rebounded to 600 mOsm. Remarkably, these changes affect the two independent regulatory networks that promote acquisition (Hk1-Rrp1) and transmission (Rrp2-RpoN-RpoS) of B. burgdorferi. Increased osmolarity affected morphology and motility of wild-type strains, and lysed Hk1 and Rrp1 mutant strains. At low osmolarity, Borrelia cells express increased levels of RpoN-RpoS-dependent virulence factors (OspC, DbpA) required for the mammalian infection. Our results strongly suggest that osmolarity is an important part of the recognized signals that allow the bacteria to adjust gene expression during the acquisition and transmission phases of the infective cycle of B. burgdorferi.", "link"=>"http://www.mendeley.com/research/two-different-virulencerelated-regulatory-pathways-borrelia-burgdorferi-directly-affected-osmotic-fl", "reader_count"=>10, "reader_count_by_academic_status"=>{"Librarian"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>3, "Student > Master"=>3, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Librarian"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>3, "Student > Master"=>3, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Nursing and Health Professions"=>1, "Agricultural and Biological Sciences"=>5, "Philosophy"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}, "Philosophy"=>{"Philosophy"=>1}}, "reader_count_by_country"=>{"United Kingdom"=>1}, "group_count"=>1}

Scopus | Further Information

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