Mendeley | Further Information

{"title"=>"Single-Molecule FISH Reveals Non-selective Packaging of Rift Valley Fever Virus Genome Segments", "type"=>"journal", "authors"=>[{"first_name"=>"Paul J.", "last_name"=>"Wichgers Schreur", "scopus_author_id"=>"33167846300"}, {"first_name"=>"Jeroen", "last_name"=>"Kortekaas", "scopus_author_id"=>"35280439500"}], "year"=>2016, "source"=>"PLoS Pathogens", "identifiers"=>{"sgr"=>"84984799231", "doi"=>"10.1371/journal.ppat.1005800", "pui"=>"611987925", "pmid"=>"27548280", "scopus"=>"2-s2.0-84984799231", "issn"=>"15537374", "isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)"}, "id"=>"8d6fcfcd-8d35-3c0d-88e3-794361a2339f", "abstract"=>"The bunyavirus genome comprises a small (S), medium (M), and large (L) RNA segment of negative polarity. Although genome segmentation confers evolutionary advantages by enabling genome reassortment events with related viruses, genome segmentation also complicates genome replication and packaging. Accumulating evidence suggests that genomes of viruses with eight or more genome segments are incorporated into virions by highly selective processes. Remarkably, little is known about the genome packaging process of the tri-segmented bunyaviruses. Here, we evaluated, by single-molecule RNA fluorescence in situ hybridization (FISH), the intracellular spatio-temporal distribution and replication kinetics of the Rift Valley fever virus (RVFV) genome and determined the segment composition of mature virions. The results reveal that the RVFV genome segments start to replicate near the site of infection before spreading and replicating throughout the cytoplasm followed by translocation to the virion assembly site at the Golgi network. Despite the average intracellular S, M and L genome segments approached a 1:1:1 ratio, major differences in genome segment ratios were observed among cells. We also observed a significant amount of cells lacking evidence of M-segment replication. Analysis of two-segmented replicons and four-segmented viruses subsequently confirmed the previous notion that Golgi recruitment is mediated by the Gn glycoprotein. The absence of colocalization of the different segments in the cytoplasm and the successful rescue of a tri-segmented variant with a codon shuffled M-segment suggested that inter-segment interactions are unlikely to drive the copackaging of the different segments into a single virion. The latter was confirmed by direct visualization of RNPs inside mature virions which showed that the majority of virions lack one or more genome segments. Altogether, this study suggests that RVFV genome packaging is a non-selective process.", "link"=>"http://www.mendeley.com/research/singlemolecule-fish-reveals-nonselective-packaging-rift-valley-fever-virus-genome-segments", "reader_count"=>27, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>14, "Student > Master"=>3, "Student > Bachelor"=>3, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>14, "Student > Master"=>3, "Student > Bachelor"=>3, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>15, "Veterinary Science and Veterinary Medicine"=>2, "Immunology and Microbiology"=>2, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>15}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>2}}, "group_count"=>1}

CrossRef

• http://doi.org/10.1128/JVI.00743-17
• http://doi.org/10.1099/jgv.0.000930
• http://doi.org/10.1038/s41467-017-00102-9
• http://doi.org/10.1371/journal.ppat.1005819
• http://doi.org/10.1128/JVI.02241-17
• http://doi.org/10.1146/annurev-virology-092818-015652
• http://doi.org/10.1016/j.tim.2017.02.003
• http://doi.org/10.3390/v12030351
• http://doi.org/10.1016/j.coviro.2018.06.001
• http://doi.org/10.1371/journal.ppat.1007200
• http://doi.org/10.1021/acsinfecdis.0c00402
• http://doi.org/10.1146/annurev-virology-010220-063346
• http://doi.org/10.1128/JVI.01164-17
• http://doi.org/10.1089/vbz.2018.2383
• http://doi.org/10.1016/j.virusres.2017.01.018
• http://doi.org/10.1002/rmv.2039
• http://doi.org/10.1016/j.jviromet.2019.113701
• http://doi.org/10.1007/s12250-020-00319-x
• http://doi.org/10.1186/s12985-018-0932-8
• http://doi.org/10.1007/s11262-018-1611-y
• http://doi.org/10.1242/jcs.252544
• http://doi.org/10.1007/s12038-018-9769-y
• http://doi.org/10.1111/cmi.12765
• http://doi.org/10.1016/j.coviro.2018.08.011
• http://doi.org/10.1080/1040841X.2017.1307805
• http://doi.org/10.1128/JVI.00968-17
• http://doi.org/10.1099/jgv.0.001418
• http://doi.org/10.7554/eLife.43599
• http://doi.org/10.1038/s41579-020-00449-9
• http://doi.org/10.1093/ve/vez014
• http://doi.org/10.1016/j.vaccine.2017.04.024
• http://doi.org/10.1080/1040841X.2018.1446901

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84984799231"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84984799231?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84984799231&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84984799231&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84984799231", "dc:identifier"=>"SCOPUS_ID:84984799231", "eid"=>"2-s2.0-84984799231", "dc:title"=>"Single-Molecule FISH Reveals Non-selective Packaging of Rift Valley Fever Virus Genome Segments", "dc:creator"=>"Wichgers Schreur P.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"12", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2016-08-01", "prism:coverDisplayDate"=>"August 2016", "prism:doi"=>"10.1371/journal.ppat.1005800", "citedby-count"=>"32", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Wageningen University & Research", "affiliation-city"=>"Wageningen", "affiliation-country"=>"Netherlands"}], "pubmed-id"=>"27548280", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1005800", "source-id"=>"4000151809", "openaccess"=>"1", "openaccessFlag"=>true, "freetoread"=>{"value"=>[{"$"=>"all"}, {"$"=>"publisherfullgold"}, {"$"=>"repository"}, {"$"=>"repositoryvor"}, {"$"=>"repositoryam"}]}, "freetoreadLabel"=>{"value"=>[{"$"=>"All Open Access"}, {"$"=>"Gold"}, {"$"=>"Green"}]}}

Web of Science® | Further Information

Article Coverage Curated

Journal Comments

Twitter

• {"id"=>"767843984622297090", "text"=>"New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI https://t.co/5l20o9fSE3", "created_at"=>"2016-08-22T22:00:46Z", "user"=>"PLOSPathogens", "user_name"=>"PLOS Pathogens", "user_profile_image"=>"http://pbs.twimg.com/profile_images/459394339945197568/BuJQyL0D_normal.jpeg"}
• {"id"=>"767844237849272322", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-22T22:01:46Z", "user"=>"GheorghePaul", "user_name"=>"Dr George Paul", "user_profile_image"=>"http://pbs.twimg.com/profile_images/3346366493/5ac1e111ae9224b576f7c1a33dc905d3_normal.jpeg"}
• {"id"=>"767846482695319552", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-22T22:10:42Z", "user"=>"BIOCIENCIA2013", "user_name"=>"BIOCIENCIA", "user_profile_image"=>"http://pbs.twimg.com/profile_images/378800000300217090/9b25d5f581a95fff46afd4d8d0d0aad0_normal.jpeg"}
• {"id"=>"767846777869312001", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-22T22:11:52Z", "user"=>"ainakosiboyie", "user_name"=>"Amado Guloy", "user_profile_image"=>"http://pbs.twimg.com/profile_images/601818675969036289/bdjPgtda_normal.jpg"}
• {"id"=>"767856019171057664", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-22T22:48:35Z", "user"=>"m0niquemerchant", "user_name"=>"Monique Merchant", "user_profile_image"=>"http://pbs.twimg.com/profile_images/740543609011904512/HspQTh4n_normal.jpg"}
• {"id"=>"767857211615830016", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-22T22:53:20Z", "user"=>"No_See_Em", "user_name"=>"Emily McDermott", "user_profile_image"=>"http://pbs.twimg.com/profile_images/745376816727101440/verX8y50_normal.jpg"}
• {"id"=>"767873908745838593", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-22T23:59:40Z", "user"=>"sdelagrave", "user_name"=>"Simon Delagrave", "user_profile_image"=>"http://pbs.twimg.com/profile_images/551478113939365888/dlzIg_3M_normal.jpeg"}
• {"id"=>"767877641894522882", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T00:14:30Z", "user"=>"JoanneLiu_MSF", "user_name"=>"Dr Joanne Liu", "user_profile_image"=>"http://pbs.twimg.com/profile_images/540428589561163776/avJ9fm2p_normal.jpeg"}
• {"id"=>"767877842042486784", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T00:15:18Z", "user"=>"Mesihceyhun", "user_name"=>"Ceyhun Can Ate?", "user_profile_image"=>"http://pbs.twimg.com/profile_images/761575882242789376/XyK-kcM__normal.jpg"}
• {"id"=>"767879910312538116", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T00:23:31Z", "user"=>"mip_MIIP", "user_name"=>"MireilleP", "user_profile_image"=>"http://pbs.twimg.com/profile_images/566820857180598272/ml0qgoEc_normal.jpeg"}
• {"id"=>"767892328270946305", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T01:12:52Z", "user"=>"dmrmay", "user_name"=>"Mr. D.", "user_profile_image"=>"http://pbs.twimg.com/profile_images/585263515339988992/m6QUT7eJ_normal.jpg"}
• {"id"=>"767915142944219136", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T02:43:31Z", "user"=>"NathanGrubaugh", "user_name"=>"Nathan Grubaugh", "user_profile_image"=>"http://pbs.twimg.com/profile_images/717485571858255872/_5dQAOPD_normal.jpg"}
• {"id"=>"767938341085978624", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T04:15:42Z", "user"=>"kororoad1", "user_name"=>"Kororoad", "user_profile_image"=>"http://abs.twimg.com/sticky/default_profile_images/default_profile_2_normal.png"}
• {"id"=>"767997341110562816", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T08:10:09Z", "user"=>"verorezelj", "user_name"=>"Veronica Rezelj", "user_profile_image"=>"http://pbs.twimg.com/profile_images/710014734637076480/lAUqWvwE_normal.jpg"}
• {"id"=>"768001329667121156", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T08:26:00Z", "user"=>"nathcharbonnel", "user_name"=>"CharbonnelNath", "user_profile_image"=>"http://pbs.twimg.com/profile_images/708017584134868992/FN4HA2eU_normal.jpg"}
• {"id"=>"768019812261982209", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T09:39:27Z", "user"=>"bunyavax", "user_name"=>"Jeroen Kortekaas", "user_profile_image"=>"http://pbs.twimg.com/profile_images/478234479417253890/a1JNM_9t_normal.jpeg"}
• {"id"=>"768039502103404544", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T10:57:41Z", "user"=>"MASP1982", "user_name"=>"Flavius ??????????", "user_profile_image"=>"http://pbs.twimg.com/profile_images/757390889782284288/7MZt6BtS_normal.jpg"}
• {"id"=>"768142379291447296", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T17:46:29Z", "user"=>"LydiaMFanfan", "user_name"=>"Lydia Maria Fanfan", "user_profile_image"=>"http://pbs.twimg.com/profile_images/541415858749583360/B1RSfqYG_normal.jpeg"}
• {"id"=>"768198557505585153", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T21:29:43Z", "user"=>"tgarretson82", "user_name"=>"Tyler Garretson", "user_profile_image"=>"http://pbs.twimg.com/profile_images/378800000214850027/fd9bff6baff41a6fae23229de09f0a4b_normal.jpeg"}
• {"id"=>"768204841978060801", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-23T21:54:41Z", "user"=>"DukeMGM", "user_name"=>"DukeMGM", "user_profile_image"=>"http://pbs.twimg.com/profile_images/424454668/images_normal.jpeg"}
• {"id"=>"768373615339462656", "text"=>"Single-molecule FISH reveals non-selective packaging of Rift Valley fever virus genome segments https://t.co/3HVELdfGyh #midweekmicro", "created_at"=>"2016-08-24T09:05:20Z", "user"=>"NatureRevMicro", "user_name"=>"Nature Rev Microbiol", "user_profile_image"=>"http://pbs.twimg.com/profile_images/480994211626373120/jxmDYIr2_normal.jpeg"}
• {"id"=>"768391263569776640", "text"=>"RT @NatureRevMicro: Single-molecule FISH reveals non-selective packaging of Rift Valley fever virus genome segments https://t.co/3HVELdfGyh…", "created_at"=>"2016-08-24T10:15:27Z", "user"=>"cjblondel", "user_name"=>"Carlos J. Blondel", "user_profile_image"=>"http://pbs.twimg.com/profile_images/560296001806680064/baIXgrQ5_normal.jpeg"}
• {"id"=>"768420902040064000", "text"=>"RT @NatureRevMicro: Single-molecule FISH reveals non-selective packaging of Rift Valley fever virus genome segments https://t.co/3HVELdfGyh…", "created_at"=>"2016-08-24T12:13:14Z", "user"=>"Kikyoeduardo", "user_name"=>"Eduardo Hern?ndez", "user_profile_image"=>"http://pbs.twimg.com/profile_images/641160096/IMG0007C_normal.jpg"}
• {"id"=>"768474308133789696", "text"=>"RT @NatureRevMicro: Single-molecule FISH reveals non-selective packaging of Rift Valley fever virus genome segments https://t.co/3HVELdfGyh…", "created_at"=>"2016-08-24T15:45:27Z", "user"=>"ifreecell", "user_name"=>"freecell", "user_profile_image"=>"http://pbs.twimg.com/profile_images/469262786527510528/8ylSUQU2_normal.jpeg"}
• {"id"=>"768553292758671361", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-24T20:59:18Z", "user"=>"CVRinfo", "user_name"=>"CVR", "user_profile_image"=>"http://pbs.twimg.com/profile_images/1796487750/CVRlogo_normal.jpg"}
• {"id"=>"768801242554458112", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-25T13:24:34Z", "user"=>"DiagnosticChick", "user_name"=>"Isabel Ott", "user_profile_image"=>"http://pbs.twimg.com/profile_images/748645773668147200/tggxuxUt_normal.jpg"}
• {"id"=>"768806760165548032", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-08-25T13:46:30Z", "user"=>"edrybicki", "user_name"=>"Ed Rybicki", "user_profile_image"=>"http://pbs.twimg.com/profile_images/946568031/ed_2010_normal.jpg"}
• {"id"=>"768816192534507520", "text"=>"This one picture: SO cool! Definitely worth 10exp3 words! @DiagnosticChick https://t.co/JFXIU7eZK2 https://t.co/MjOHNdXhju", "created_at"=>"2016-08-25T14:23:58Z", "user"=>"edrybicki", "user_name"=>"Ed Rybicki", "user_profile_image"=>"http://pbs.twimg.com/profile_images/946568031/ed_2010_normal.jpg"}
• {"id"=>"769280567942770689", "text"=>"Single-Molecule FISH Reveals Non-selective Packaging of Rift Valley Fever Virus Genome Segments https://t.co/LUFczCbAQc", "created_at"=>"2016-08-26T21:09:14Z", "user"=>"BIOCIENCIA2013", "user_name"=>"BIOCIENCIA", "user_profile_image"=>"http://pbs.twimg.com/profile_images/378800000300217090/9b25d5f581a95fff46afd4d8d0d0aad0_normal.jpeg"}
• {"id"=>"770241050145349632", "text"=>"Cool paper on Rift Valley Fever genome packaging into virions:\nhttps://t.co/YTefXvIf3k", "created_at"=>"2016-08-29T12:45:51Z", "user"=>"bmainou", "user_name"=>"Bernardo Mainou", "user_profile_image"=>"http://pbs.twimg.com/profile_images/3131241038/c9bc55aada9315de93d6fe3577a59653_normal.jpeg"}
• {"id"=>"771162427136151552", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2016-09-01T01:47:04Z", "user"=>"BenteLab", "user_name"=>"Dennis Bente", "user_profile_image"=>"http://pbs.twimg.com/profile_images/479758891652116480/3j4wNtZO_normal.jpeg"}
• {"id"=>"836686921451651073", "text"=>"they can't even package 3 unique genome segments together consistently. it's absurd. (from https://t.co/7khu4XjLUm ) https://t.co/byjMlsscL5", "created_at"=>"2017-02-28T21:18:01Z", "user"=>"DiagnosticChick", "user_name"=>"Isabel Ott", "user_profile_image"=>"http://pbs.twimg.com/profile_images/800779584715124736/xQlT-UZY_normal.jpg"}
• {"id"=>"861569056234754049", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2017-05-08T13:10:44Z", "user"=>"igoodfel", "user_name"=>"Ian Goodfellow", "user_profile_image"=>"http://pbs.twimg.com/profile_images/547796722106503169/ZXOFLXyA_normal.jpeg"}
• {"id"=>"861570959572160512", "text"=>"RT @PLOSPathogens: New research from @bunyavax & co on visualizing genome packaging in Rift Valley Fever Virus https://t.co/6Cf0fxs0XI http…", "created_at"=>"2017-05-08T13:18:18Z", "user"=>"imparnham", "user_name"=>"Ian M. Parnham", "user_profile_image"=>"http://pbs.twimg.com/profile_images/841993641971216384/KFN4G12e_normal.jpg"}

Wikipedia

Counter

• {"month"=>"8", "year"=>"2016", "pdf_views"=>"135", "xml_views"=>"0", "html_views"=>"1409"}
• {"month"=>"9", "year"=>"2016", "pdf_views"=>"134", "xml_views"=>"0", "html_views"=>"462"}
• {"month"=>"10", "year"=>"2016", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"94"}
• {"month"=>"11", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"115"}
• {"month"=>"12", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"69"}
• {"month"=>"1", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"44"}
• {"month"=>"2", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"59"}
• {"month"=>"3", "year"=>"2017", "pdf_views"=>"38", "xml_views"=>"2", "html_views"=>"92"}
• {"month"=>"4", "year"=>"2017", "pdf_views"=>"32", "xml_views"=>"1", "html_views"=>"107"}
• {"month"=>"5", "year"=>"2017", "pdf_views"=>"39", "xml_views"=>"0", "html_views"=>"338"}
• {"month"=>"6", "year"=>"2017", "pdf_views"=>"59", "xml_views"=>"0", "html_views"=>"321"}
• {"month"=>"7", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"330"}
• {"month"=>"8", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"264"}
• {"month"=>"9", "year"=>"2017", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"140"}
• {"month"=>"10", "year"=>"2017", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"52"}
• {"month"=>"11", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"60"}
• {"month"=>"12", "year"=>"2017", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"47"}
• {"month"=>"1", "year"=>"2018", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"57"}
• {"month"=>"2", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"40"}
• {"month"=>"3", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"46"}
• {"month"=>"4", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"31"}
• {"month"=>"5", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"28"}
• {"month"=>"6", "year"=>"2018", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"29"}
• {"month"=>"7", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"3", "html_views"=>"22"}
• {"month"=>"8", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"29"}
• {"month"=>"9", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"33"}
• {"month"=>"10", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"31"}
• {"month"=>"11", "year"=>"2018", "pdf_views"=>"28", "xml_views"=>"1", "html_views"=>"39"}
• {"month"=>"12", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"19"}
• {"month"=>"1", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"29"}
• {"month"=>"2", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"27"}
• {"month"=>"3", "year"=>"2019", "pdf_views"=>"39", "xml_views"=>"1", "html_views"=>"155"}
• {"month"=>"4", "year"=>"2019", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"35"}
• {"month"=>"5", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"31"}
• {"month"=>"6", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"21"}
• {"month"=>"7", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"29"}
• {"month"=>"8", "year"=>"2019", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"19"}
• {"month"=>"9", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"36"}
• {"month"=>"10", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"34"}
• {"month"=>"11", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"30"}
• {"month"=>"12", "year"=>"2019", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"29"}
• {"month"=>"1", "year"=>"2020", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"25"}
• {"month"=>"2", "year"=>"2020", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"43"}
• {"month"=>"3", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"32"}
• {"month"=>"4", "year"=>"2020", "pdf_views"=>"49", "xml_views"=>"1", "html_views"=>"33"}
• {"month"=>"5", "year"=>"2020", "pdf_views"=>"51", "xml_views"=>"0", "html_views"=>"44"}
• {"month"=>"6", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"9"}
• {"month"=>"7", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
• {"month"=>"8", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"23"}
• {"month"=>"9", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"28"}
• {"month"=>"10", "year"=>"2020", "pdf_views"=>"26", "xml_views"=>"2", "html_views"=>"24"}
• {"month"=>"11", "year"=>"2020", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"21"}
• {"month"=>"12", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"25"}
• {"month"=>"1", "year"=>"2021", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"24"}
• {"month"=>"2", "year"=>"2021", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"20"}
• {"month"=>"3", "year"=>"2021", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}

PMC Usage Stats

• {"unique-ip"=>"15", "full-text"=>"27", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
• {"unique-ip"=>"25", "full-text"=>"23", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"25", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
• {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
• {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
• {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
• {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
• {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
• {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
• {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
• {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
• {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
• {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
• {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
• {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
• {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"11"}
• {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
• {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
• {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
• {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
• {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
• {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
• {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
• {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
• {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
• {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
• {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
• {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
• {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
• {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
• {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
• {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
• {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
• {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
• {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
• {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
• {"unique-ip"=>"10", "full-text"=>"5", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"12"}
• {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
• {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
• {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
• {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
• {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
• {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
• {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
• {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
• {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}
• {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"11", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"11"}
• {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"12"}
• {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2021", "month"=>"1"}

F1000Prime