Single-cell analysis identifies cellular markers of the HIV permissive cell
Publication Date
October 26, 2017
Authors
Sylvie Rato, Antonio Rausell, Miguel Muñoz, Amalio Telenti, et al
Volume
13
Issue
10
Pages
e1006678
DOI
https://dx.plos.org/10.1371/journal.ppat.1006678
Publisher URL
http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1006678
Scopus
85033226512
Mendeley
http://www.mendeley.com/research/singlecell-analysis-identifies-cellular-markers-hiv-permissive-cell
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Mendeley | Further Information

{"title"=>"Single-cell analysis identifies cellular markers of the HIV permissive cell", "type"=>"journal", "authors"=>[{"first_name"=>"Sylvie", "last_name"=>"Rato", "scopus_author_id"=>"14219533300"}, {"first_name"=>"Antonio", "last_name"=>"Rausell", "scopus_author_id"=>"14029161200"}, {"first_name"=>"Miguel", "last_name"=>"Muñoz", "scopus_author_id"=>"21737624800"}, {"first_name"=>"Amalio", "last_name"=>"Telenti", "scopus_author_id"=>"7006764406"}, {"first_name"=>"Angela", "last_name"=>"Ciuffi", "scopus_author_id"=>"9534445600"}], "year"=>2017, "source"=>"PLoS Pathogens", "identifiers"=>{"scopus"=>"2-s2.0-85033226512", "pui"=>"619157835", "sgr"=>"85033226512", "isbn"=>"1111111111", "issn"=>"15537374", "pmid"=>"29073251", "doi"=>"10.1371/journal.ppat.1006678"}, "id"=>"d2d826ed-a56c-3a9c-8500-7d5037d11795", "abstract"=>"Cellular permissiveness to HIV infection is highly heterogeneous across individuals. Heterogeneity is also found across CD4+ T cells from the same individual, where only a fraction of cells gets infected. To explore the basis of permissiveness, we performed single-cell RNA-seq analysis of non-infected CD4+ T cells from high and low permissive individuals. Transcriptional heterogeneity translated in a continuum of cell states, driven by T-cell receptor-mediated cell activation and was strongly linked to permissiveness. Proteins expressed at the cell surface and displaying the highest correlation with T cell activation were tested as biomarkers of cellular permissiveness to HIV. FACS sorting using antibodies against several biomarkers of permissiveness led to an increase of HIV cellular infection rates. Top candidate biomarkers included CD25, a canonical activation marker. The combination of CD25 high expression with other candidate biomarkers led to the identification of CD298, CD63 and CD317 as the best biomarkers for permissiveness. CD25highCD298highCD63highCD317high cell population showed an enrichment of HIV-infection of up to 28 fold as compared to the unsorted cell population. The purified hyper-permissive cell subpopulation was characterized by a downregulation of interferon-induced genes and several known restriction factors. Single-cell RNA-seq analysis coupled with functional characterization of cell biomarkers provides signatures of the “HIV-permissive cell”.", "link"=>"http://www.mendeley.com/research/singlecell-analysis-identifies-cellular-markers-hiv-permissive-cell", "reader_count"=>36, "reader_count_by_academic_status"=>{"Unspecified"=>6, "Student > Doctoral Student"=>2, "Researcher"=>6, "Student > Ph. D. Student"=>10, "Student > Master"=>4, "Student > Bachelor"=>5, "Lecturer"=>1, "Professor"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>6, "Student > Doctoral Student"=>2, "Researcher"=>6, "Student > Ph. D. Student"=>10, "Student > Master"=>4, "Student > Bachelor"=>5, "Lecturer"=>1, "Professor"=>1, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>6, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>11, "Medicine and Dentistry"=>4, "Agricultural and Biological Sciences"=>9, "Chemistry"=>1, "Immunology and Microbiology"=>4}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>11}, "Unspecified"=>{"Unspecified"=>6}, "Environmental Science"=>{"Environmental Science"=>1}}, "group_count"=>0}

Scopus | Further Information

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