Structural basis of mammalian glycan targeting by Vibrio cholerae cytolysin and biofilm proteins
Publication Date
February 12, 2018
Authors
Swastik De, Katherine Kaus, Shada Sinclair, Brandon C. Case, et al
Volume
14
Issue
2
Pages
e1006841
DOI
https://dx.plos.org/10.1371/journal.ppat.1006841
Publisher URL
http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1006841
Scopus
85042725718
Mendeley
http://www.mendeley.com/research/structural-basis-mammalian-glycan-targeting-vibrio-cholerae-cytolysin-biofilm-proteins
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Structural basis of mammalian glycan targeting by Vibrio cholerae cytolysin and biofilm proteins", "type"=>"journal", "authors"=>[{"first_name"=>"Swastik", "last_name"=>"De", "scopus_author_id"=>"53663334100"}, {"first_name"=>"Katherine", "last_name"=>"Kaus", "scopus_author_id"=>"56266492300"}, {"first_name"=>"Shada", "last_name"=>"Sinclair", "scopus_author_id"=>"57200965729"}, {"first_name"=>"Brandon C.", "last_name"=>"Case", "scopus_author_id"=>"57200958527"}, {"first_name"=>"Rich", "last_name"=>"Olson", "scopus_author_id"=>"56822958100"}], "year"=>2018, "source"=>"PLoS Pathogens", "identifiers"=>{"pmid"=>"29432487", "sgr"=>"85042725718", "doi"=>"10.1371/journal.ppat.1006841", "scopus"=>"2-s2.0-85042725718", "pui"=>"620981939", "isbn"=>"1111111111", "issn"=>"15537374"}, "id"=>"35cf534c-61c4-3f04-85d2-f4db21e7c468", "abstract"=>"Vibrio cholerae is an aquatic gram-negative microbe responsible for cholera, a pandemic disease causing life-threatening diarrheal outbreaks in populations with limited access to health care. Like most pathogenic bacteria, V. cholerae secretes virulence factors to assist colonization of human hosts, several of which bind carbohydrate receptors found on cell-surfaces. Understanding how pathogenic virulence proteins specifically target host cells is important for the development of treatment strategies to fight bacterial infections. Vibrio cholerae cytolysin (VCC) is a secreted pore-forming toxin with a carboxy-terminal β-prism domain that targets complex N-glycans found on mammalian cell-surface proteins. To investigate glycan selectivity, we studied the VCC β-prism domain and two additional β-prism domains found within the V. cholerae biofilm matrix protein RbmC. We show that the two RbmC β-prism domains target a similar repertoire of complex N-glycan receptors as VCC and find through binding and modeling studies that a branched pentasaccharide core (GlcNAc2-Man3) represents the likely footprint interacting with these domains. To understand the structural basis of V. cholerae β-prism selectivity, we solved high-resolution crystal structures of fragments of the pentasaccharide core bound to one RbmC β-prism domain and conducted mutagenesis experiments on the VCC toxin. Our results highlight a common strategy for cell-targeting utilized by both toxin and biofilm matrix proteins in Vibrio cholerae and provide a structural framework for understanding the specificity for individual receptors. Our results suggest that a common strategy for disrupting carbohydrate interactions could affect multiple virulence factors produced by V. cholerae, as well as similar β-prism domains found in other vibrio pathogens.", "link"=>"http://www.mendeley.com/research/structural-basis-mammalian-glycan-targeting-vibrio-cholerae-cytolysin-biofilm-proteins", "reader_count"=>5, "reader_count_by_academic_status"=>{"Student > Ph. D. Student"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Student > Ph. D. Student"=>1, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85042725718"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85042725718?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042725718&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85042725718&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85042725718", "dc:identifier"=>"SCOPUS_ID:85042725718", "eid"=>"2-s2.0-85042725718", "dc:title"=>"Structural basis of mammalian glycan targeting by Vibrio cholerae cytolysin and biofilm proteins", "dc:creator"=>"De S.", "prism:publicationName"=>"PLoS Pathogens", "prism:issn"=>"15537366", "prism:eIssn"=>"15537374", "prism:volume"=>"14", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2018-02-01", "prism:coverDisplayDate"=>"February 2018", "prism:doi"=>"10.1371/journal.ppat.1006841", "citedby-count"=>"0", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Wesleyan University Middletown", "affiliation-city"=>"Middletown", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"Yale University", "affiliation-city"=>"New Haven", "affiliation-country"=>"United States"}], "pubmed-id"=>"29432487", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e1006841", "source-id"=>"4000151809", "openaccess"=>"1", "openaccessFlag"=>true}

Counter

  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"107", "xml_views"=>"10", "html_views"=>"470"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"46", "xml_views"=>"4", "html_views"=>"141"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"33", "xml_views"=>"3", "html_views"=>"80"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"51", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"17"}

PMC Usage Stats

  • {"unique-ip"=>"18", "full-text"=>"14", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
Loading … Spinner
There are currently no alerts