CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection
Publication Date
March 08, 2018
Authors
Yuemei Dong, Maria L. Simões, Eric Marois & George Dimopoulos
Volume
14
Issue
3
Pages
e1006898
DOI
http://doi.org/10.1371/journal.ppat.1006898
Publisher URL
http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1006898
Scopus
85044827125
Mendeley
http://www.mendeley.com/research/crisprcas9-mediated-gene-knockout-anopheles-gambiae-frep1-suppresses-malaria-parasite-infection
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Mendeley | Further Information

{"title"=>"CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection", "type"=>"journal", "authors"=>[{"first_name"=>"Yuemei", "last_name"=>"Dong", "scopus_author_id"=>"8684370700"}, {"first_name"=>"Maria L.", "last_name"=>"Simões", "scopus_author_id"=>"56763526600"}, {"first_name"=>"Eric", "last_name"=>"Marois", "scopus_author_id"=>"7801401737"}, {"first_name"=>"George", "last_name"=>"Dimopoulos", "scopus_author_id"=>"19337048900"}], "year"=>2018, "source"=>"PLoS Pathogens", "identifiers"=>{"sgr"=>"85044827125", "pui"=>"621543449", "issn"=>"15537374", "isbn"=>"1111111111", "scopus"=>"2-s2.0-85044827125", "doi"=>"10.1371/journal.ppat.1006898"}, "id"=>"56a18cea-b9d0-3d88-8198-7c6f62be3c62", "abstract"=>"Plasmodium relies on numerous agonists during its journey through the mosquito vector, and these agonists represent potent targets for transmission-blocking by either inhibiting or interfering with them pre-or post-transcriptionally. The recently developed CRISPR/Cas9-based genome editing tools for Anopheles mosquitoes provide new and promising opportu-nities for the study of agonist function and for developing malaria control strategies through gene deletion to achieve complete agonist inactivation. Here we have established a modi-fied CRISPR/Cas9 gene editing procedure for the malaria vector Anopheles gambiae, and studied the effect of inactivating the fibrinogen-related protein 1 (FREP1) gene on the mos-quito's susceptibility to Plasmodium and on mosquito fitness. FREP1 knockout mutants developed into adult mosquitoes that showed profound suppression of infection with both human and rodent malaria parasites at the oocyst and sporozoite stages. FREP1 inactiva-tion, however, resulted in fitness costs including a significantly lower blood-feeding propen-sity, fecundity and egg hatching rate, a retarded pupation time, and reduced longevity after a blood meal. Author summary", "link"=>"http://www.mendeley.com/research/crisprcas9-mediated-gene-knockout-anopheles-gambiae-frep1-suppresses-malaria-parasite-infection", "reader_count"=>33, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>7, "Other"=>2, "Student > Bachelor"=>4, "Professor"=>2, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>7, "Other"=>2, "Student > Bachelor"=>4, "Professor"=>2, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>12, "Agricultural and Biological Sciences"=>13, "Medicine and Dentistry"=>2, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>13}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}, "Unspecified"=>{"Unspecified"=>4}}, "group_count"=>1}

Scopus | Further Information

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