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{"title"=>"ZMIZ1 preferably enhances the transcriptional activity of androgen receptor with short polyglutamine tract", "type"=>"journal", "authors"=>[{"first_name"=>"Xiaomeng", "last_name"=>"Li", "scopus_author_id"=>"9532654300"}, {"first_name"=>"Chunfang", "last_name"=>"Zhu", "scopus_author_id"=>"55552938500"}, {"first_name"=>"William H.", "last_name"=>"Tu", "scopus_author_id"=>"7006479248"}, {"first_name"=>"Nanyang", "last_name"=>"Yang", "scopus_author_id"=>"26323948300"}, {"first_name"=>"Hui", "last_name"=>"Qin", "scopus_author_id"=>"53164791500"}, {"first_name"=>"Zijie", "last_name"=>"Sun", "scopus_author_id"=>"7404240329"}], "year"=>2011, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"21949845", "sgr"=>"80052946890", "scopus"=>"2-s2.0-80052946890", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0025040", "pui"=>"362585445", "issn"=>"19326203"}, "id"=>"b5aca3b4-4bc4-3584-bbe3-eb7b4301b31e", "abstract"=>"The androgen receptor (AR) is a ligand-induced transcription factor and contains the polyglutamine (polyQ) tracts within its N-terminal transactivation domain. The length of polyQ tracts has been suggested to alter AR transcriptional activity in prostate cancer along with other endocrine and neurologic disorders. Here, we assessed the role of ZMIZ1, an AR co-activator, in regulating the activity of the AR with different lengths of polyQ tracts as ARQ9, ARQ24, and ARQ35 in prostate cancer cells. ZMIZ1, but not ZMIZ2 or ARA70, preferably augments ARQ9 induced androgen-dependent transcription on three different androgen-inducible promoter/reporter vectors. A strong protein-protein interaction between ZMIZ1 and ARQ9 proteins was shown by immunoprecipitation assays. In the presence of ZMIZ1, the N and C-terminal interaction of the ARQ9 was more pronounced than ARQ24 and ARQ35. Both Brg1 and BAF57, the components of SWI/SNF complexes, were shown to be involved in the enhancement of ZMIZ1 on AR activity. Using the chromatin immunoprecipitation assays (ChIP), we further demonstrated a strong recruitment of ZMIZ1 by ARQ9 on the promoter of the prostate specific antigen (PSA) gene. These results demonstrate a novel regulatory role of ZMIZ1 in modulating the polyQ tract length of AR in prostate cancer cells.", "link"=>"http://www.mendeley.com/research/zmiz1-preferably-enhances-transcriptional-activity-androgen-receptor-short-polyglutamine-tract", "reader_count"=>16, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Master"=>2, "Student > Bachelor"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Master"=>2, "Student > Bachelor"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Mathematics"=>1, "Agricultural and Biological Sciences"=>12, "Medicine and Dentistry"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Mathematics"=>{"Mathematics"=>1}}, "reader_count_by_country"=>{"Norway"=>1}, "group_count"=>1}

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• http://doi.org/10.1038/s41598-019-44991-w
• http://doi.org/10.1038/tp.2014.138
• http://doi.org/10.1186/s12864-019-6047-9
• http://doi.org/10.1186/s13059-014-0578-9
• http://doi.org/10.1007/s40242-013-2248-y
• http://doi.org/10.1002/ijc.32587
• http://doi.org/10.1074/jbc.M113.529727
• http://doi.org/10.1016/j.ajhg.2018.03.023
• http://doi.org/10.1158/1078-0432.CCR-18-0101
• http://doi.org/10.1016/j.ajhg.2018.12.007
• http://doi.org/10.1371/journal.pgen.1008451

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Figshare

• {"files"=>["https://ndownloader.figshare.com/files/733377"], "description"=>"<p>DU145 cells were transiently transfected in 48-well plates with 100 ng of 7 kb PSA-Luc, 25 ng of pSV40-β-gal, 5 ng of ARQ9, Q24, or Q35, and 10 or 30 ng of pcDNA3-FLAG-hZMIZ1, pcDNA3-FLAG-hZMIZ2, or pcDNA3-FLAG-ARA70 as where are indicated. The total amount of plasmids per well was normalized in all transfections by the addition of pcDNA3 empty vector. Twenty-four hours after transfection, cells were incubated with or without 1 nM DHT for another 24 hours and then whole cell lysates were prepared for assessment of luciferase and β-gal activities. Transfection experiments were repeated a minimum of three times in triplicate. Relative luciferase units (RLUs) were determined from three independent transfections and are presented as the mean ± SEM of the triplicate transfections. “*” indicates a statistically significant difference (p<0.05) between the samples co-expressed AR and other AR co-factors in the presence of 1 nM DHT.</p>", "links"=>[], "tags"=>["selectively", "enhances", "shorter", "polyq-ar", "mediated"], "article_id"=>403724, "categories"=>["Chemistry", "Molecular Biology", "Cancer"], "users"=>["Xiaomeng Li", "Chunfang Zhu", "William H. Tu", "Nanyang Yang", "Hui Qin", "Zijie Sun"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025040.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ZMIZ1_selectively_enhances_the_shorter_polyQ_AR_mediated_transcription_/403724", "title"=>"ZMIZ1 selectively enhances the shorter polyQ-AR mediated transcription.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-20 01:02:04"}
• {"files"=>["https://ndownloader.figshare.com/files/733880"], "description"=>"<p>(A) Equal amounts of <i>in vitro</i> translated ³⁵S methionine-labeled NTD domain of ARQ9, Q24, Q35 or ARA70 were measured and used in GST pull down assays. (B) GST-AR LBD (amino acids: 676–919), GST-AR DBD (aa: 559–624), or GST protein alone, as a negative control, were isolated, purified, and subjected to binding assays. Equal amounts of the above GST proteins were analyzed on SDS-PAGE. (C) Equal amounts of the <i>in vitro</i> translated NTD of ARQ9, Q24, Q35 or ARA70 were incubated with different GST fusion proteins coupled to glutathione-Sepharose beads in the binding buffer (see the “<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025040#s2\" target=\"_blank\">Material and Methods</a>) with 10 nM DHT for 4 hours at 4°C. The beads were washed and subjected to SDS-PAGE and autoradiography. (D) DU145 cells with stable expression of different AR polyQ proteins were transfected with pcDNA3-FLAG-ZMIZ1, pcDNA3-FLAG-ZMIZ2FLAG, or pcDNA3 plasmids. Whole cell lysates were prepared from the above cells and subjected to GST pulldown experiments. Forty µl of GST-AR LBD beads were mixed with 200 µl of whole cell lysates isolated from the above DU145 cells and incubated with the binding buffer (see the “<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025040#s2\" target=\"_blank\">Material and Methods</a>) with 10 nM DHT for 4 hours at 4°C. The GST beads were washed, eluted, and subjected to SDS-PAGE and immunoblotting with an anti-AR antibody (sc-7305; Santa Cruz Biotechnology, Santa Cruz, CA). (E) Ten µl of the above cell lysates after mixed with GST-AR LBD were analyzed by Western blotting to assess levels of input from different AR polyQ, FLAG-ZMIZ1 and ARA70 proteins with anti-AR C-terminus (sc-815, Santa Cruz Biotechnology), anti-AR N-terminus (sc-816, Santa Cruz), or anti-FLAG (cat#3165, Sigma) antibody, respectively. (F) Densitometry of the membrane bands was performed, and the relative numbers were reported, as the density of the binding of AR NTD (D) normalized by the input of AR NTD proteins (the second top panel, E).</p>", "links"=>[], "tags"=>["enhances", "n-", "c-", "terminuses"], "article_id"=>404233, "categories"=>["Chemistry", "Molecular Biology", "Cancer"], "users"=>["Xiaomeng Li", "Chunfang Zhu", "William H. Tu", "Nanyang Yang", "Hui Qin", "Zijie Sun"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025040.g006", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ZMIZ1_enhances_the_interaction_between_the_N_and_C_terminuses_of_AR_/404233", "title"=>"ZMIZ1 enhances the interaction between the N- and C- terminuses of AR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-20 01:10:33"}
• {"files"=>["https://ndownloader.figshare.com/files/370366", "https://ndownloader.figshare.com/files/370399", "https://ndownloader.figshare.com/files/370462"], "description"=>"<div><p>The androgen receptor (AR) is a ligand-induced transcription factor and contains the polyglutamine (polyQ) tracts within its N-terminal transactivation domain. The length of polyQ tracts has been suggested to alter AR transcriptional activity in prostate cancer along with other endocrine and neurologic disorders. Here, we assessed the role of ZMIZ1, an AR co-activator, in regulating the activity of the AR with different lengths of polyQ tracts as ARQ9, ARQ24, and ARQ35 in prostate cancer cells. ZMIZ1, but not ZMIZ2 or ARA70, preferably augments ARQ9 induced androgen-dependent transcription on three different androgen-inducible promoter/reporter vectors. A strong protein-protein interaction between ZMIZ1 and ARQ9 proteins was shown by immunoprecipitation assays. In the presence of ZMIZ1, the N and C-terminal interaction of the ARQ9 was more pronounced than ARQ24 and ARQ35. Both Brg1 and BAF57, the components of SWI/SNF complexes, were shown to be involved in the enhancement of ZMIZ1 on AR activity. Using the chromatin immunoprecipitation assays (ChIP), we further demonstrated a strong recruitment of ZMIZ1 by ARQ9 on the promoter of the <em>prostate specific antigen</em> (<em>PSA</em>) gene. These results demonstrate a novel regulatory role of ZMIZ1 in modulating the polyQ tract length of AR in prostate cancer cells.</p> </div>", "links"=>[], "tags"=>["zmiz1", "preferably", "enhances", "transcriptional", "androgen", "receptor", "polyglutamine", "tract"], "article_id"=>133168, "categories"=>["Chemistry", "Molecular Biology", "Cancer"], "users"=>["Xiaomeng Li", "Chunfang Zhu", "William H. Tu", "Nanyang Yang", "Hui Qin", "Zijie Sun"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0025040.s001", "https://dx.doi.org/10.1371/journal.pone.0025040.s002", "https://dx.doi.org/10.1371/journal.pone.0025040.s003"], "stats"=>{"downloads"=>3, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/ZMIZ1_Preferably_Enhances_the_Transcriptional_Activity_of_Androgen_Receptor_with_Short_Polyglutamine_Tract/133168", "title"=>"ZMIZ1 Preferably Enhances the Transcriptional Activity of Androgen Receptor with Short Polyglutamine Tract", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2011-09-20 00:52:48"}
• {"files"=>["https://ndownloader.figshare.com/files/733639"], "description"=>"<p>(A) DU145 cells were transfected with a luciferase reporter driven by the human PSA promoter (100 ng), pcDNA3-β-gal (25 ng), 5 ng of ARQ9, Q24, or Q35, and where indicated, with 15 ng of pcDNA3 empty vector or pcDNA3-FLAG-ZMIZ1, different amounts of pcDNA3-Brg1 as indicated. Cells were incubated 24 h after transfection in the presence of 10 nM DHT for 24 h. The transfection experiments were repeated three times in triplicate. Relative luciferase units (RLUs) were determined from three independent transfections and are presented as the mean ± SEM of the triplicate transfections. Statistical analyses showed significant differences (p<0.05) between the samples expressed different AR Q proteins only or co-expressed AR and other co-factors in the presence of DHT, “*”. (B) DU145 cells were transfected with a luciferase reporter driven by the human PSA promoter (100 ng), pcDNA3-β-gal (25 ng), 5 ng of ARQ9, Q24, or Q35, and where indicated, with 10 ng of pcDNA3 empty vector or pcDNA3-FLAG-ZMIZ1, or different amounts of pcDNA3-Brg1 or pcDNA3-BAF57 as indicated. Cells were incubated 24 h after transfection in the presence of 10 nM DHT for 24 hr. Cell lysates were then prepared for assessment of luciferase and β-gal activities. Data were analyzed as described in (A). (C) Similar to (A), except in SW-13 cells.</p>", "links"=>[], "tags"=>["enhancement", "zmiz1", "shorter", "polyq-ar", "mediated", "transcription", "brg1"], "article_id"=>403982, "categories"=>["Chemistry", "Molecular Biology", "Cancer"], "users"=>["Xiaomeng Li", "Chunfang Zhu", "William H. Tu", "Nanyang Yang", "Hui Qin", "Zijie Sun"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025040.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_enhancement_of_ZMIZ1_on_shorter_polyQ_AR_mediated_transcription_is_associated_with_SWI_SNF_complex_Brg1_and_BAF57_/403982", "title"=>"The enhancement of ZMIZ1 on shorter polyQ-AR mediated transcription is associated with SWI/SNF complex, Brg1 and BAF57.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-20 01:06:22"}
• {"files"=>["https://ndownloader.figshare.com/files/733791"], "description"=>"<p>(A) DU145 cells stably expressing different polyQ AR proteins were transfected with pcDNA3-FLAG-ZMIZ1. Cell lysates were harvested and then immunoprecipitated with anti-FLAG antibody or normal mouse IgG. 5% input of the cell lysates was probed with the anti-FLAG, AR, or Tublin antibody. (B) Immunoprecipitates pulldowned by the AR antibody or normal IgG (IP) were analyzed by Western bolt and probed by AR and FLAG antibodies. (C) Immunoprecipitates pulldowned by the FLAG antibody and IgG (IP) were analyzed by Western blot and probed by ZMIZ1 or AR antibody.</p>", "links"=>[], "tags"=>["preferably", "binds", "ar", "polyglutamine"], "article_id"=>404138, "categories"=>["Chemistry", "Molecular Biology", "Cancer"], "users"=>["Xiaomeng Li", "Chunfang Zhu", "William H. Tu", "Nanyang Yang", "Hui Qin", "Zijie Sun"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025040.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ZMIZ1_preferably_binds_to_the_AR_protein_with_a_short_polyglutamine_tract_/404138", "title"=>"ZMIZ1 preferably binds to the AR protein with a short polyglutamine tract.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-20 01:08:58"}
• {"files"=>["https://ndownloader.figshare.com/files/733963"], "description"=>"<p>(A) Schematic diagram of the human PSA promoter region. Four pairs of PCR primers marked as A/B, C/D, E/F, and G/H are used to amplify specific AREs. (B) DU145 cells stably transfected with 7 Kb-PSA-promoter plasmids and expressing different polyQ AR were transfected with pcDNA3-FLAG-ZMIZ1 and incubated in the presence or absence of 10 nM DHT. Soluble chromatin was prepared from formaldehyde-cross-linked and sonicated cell cultures. Specific antibodies against AR (Cat# Sigma) or normal IgG as controls were used to immunoprecipitate protein-bound DNA fragments. These fragments were amplified by PCR using primers A and B. (C) The above soluble chromatin was systemically analyzed by PCR with four pairs of primers as indicated in the figure.</p>", "links"=>[], "tags"=>["enhances", "shorter", "polyq-ar", "binding", "psa"], "article_id"=>404312, "categories"=>["Chemistry", "Molecular Biology", "Cancer"], "users"=>["Xiaomeng Li", "Chunfang Zhu", "William H. Tu", "Nanyang Yang", "Hui Qin", "Zijie Sun"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025040.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ZMIZ1_enhances_shorter_polyQ_AR_binding_to_PSA_promoter_/404312", "title"=>"ZMIZ1 enhances shorter polyQ-AR binding to PSA promoter.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-20 01:11:52"}
• {"files"=>["https://ndownloader.figshare.com/files/733494"], "description"=>"<p>(A) DU145 cells were transiently transfected with 100 ng of 7 kb PSA-Luc, 25 ng of pSV40-β gal, 5 ng of ARQ9, Q24, or Q35, and where indicated, with 10 ng of pcDNA3 empty vector or pcDNA3-FLAG-hZMIZ1. Twenty-four hours after transfection, cells were incubated in the absence of DHT or in the presence of 0.1, 1, or 10 nM of DHT for 24 h. Cell lysates were prepared for assessment of luciferase and β-gal activities. The transfection experiments were repeated three times in triplicate. Relative luciferase units (RLUs) were determined from three independent transfections and are presented as the mean ± SEM of the triplicate transfections. “*” indicates a significant difference (p<0.05) between the samples co-expressed with different AR polyQ and ZMIZ1 proteins in the presence of different concentration of DHT, (B) Similar to (A), except that a MMTV-luc reporter (100 ng) was used. (C) Similar to (A), except that an ARE-luc reporter (100 ng) was used.</p>", "links"=>[], "tags"=>["enhances", "androgen-dependent", "shorter", "polyq-ar", "mediated"], "article_id"=>403841, "categories"=>["Chemistry", "Molecular Biology", "Cancer"], "users"=>["Xiaomeng Li", "Chunfang Zhu", "William H. Tu", "Nanyang Yang", "Hui Qin", "Zijie Sun"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025040.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ZMIZ1_enhances_androgen_dependent_shorter_polyQ_AR_mediated_transcription_/403841", "title"=>"ZMIZ1 enhances androgen-dependent shorter polyQ-AR mediated transcription.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-20 01:04:01"}
• {"files"=>["https://ndownloader.figshare.com/files/733734"], "description"=>"<p>DU145 cells were transfected with a luciferase reporter driven by the human PSA promoter (100 ng), pcDNA3-β-gal (25 ng), 5 ng of ARQ9, Q24, or Q35, and where indicated, with 10 ng of pcDNA3 empty vector or pcDNA3-FLAG-ZMIZ1. Twenty-four hours after transfection, cells were incubated with different androgen antagonist, including 100 nM of Bicalutamide or Flutamide for 24 h. Cell lysates were prepared for luciferase and β-gal assays. The transfection experiments were repeated three times in triplicate. Relative luciferase units (RLUs) were determined from three independent transfections and are presented as the mean ± SEM of the triplicate transfections. Statistical analyses showed significant differences (p<0.05) between the samples co-expressed with different AR polyQ and ZMIZ1 proteins in the presence of anti-androgens, “*”.</p>", "links"=>[], "tags"=>["zmiz1", "shorter", "poly-q", "ar", "insensitive", "androgen"], "article_id"=>404082, "categories"=>["Chemistry", "Molecular Biology", "Cancer"], "users"=>["Xiaomeng Li", "Chunfang Zhu", "William H. Tu", "Nanyang Yang", "Hui Qin", "Zijie Sun"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0025040.g004", "stats"=>{"downloads"=>2, "page_views"=>34, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effect_of_ZMIZ1_on_shorter_poly_Q_AR_is_insensitive_to_androgen_antagonist_/404082", "title"=>"The effect of ZMIZ1 on shorter poly-Q AR is insensitive to androgen antagonist.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2011-09-20 01:08:02"}

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F1000Prime