A Modified Method for Whole Exome Resequencing from Minimal Amounts of Starting DNA
- Publication Date
- March 05, 2012
- Journal
- PLOS ONE
- Authors
- Iwanka Kozarewa, Juan Manuel Rosa Rosa, Christopher P. Wardell, Brian A. Walker, et al
- Volume
- 7
- Issue
- 3
- Pages
- e32617
- DOI
- https://dx.plos.org/10.1371/journal.pone.0032617
- Publisher URL
- http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0032617
- PubMed
- http://www.ncbi.nlm.nih.gov/pubmed/22403682
- PubMed Central
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293839
- Europe PMC
- http://europepmc.org/abstract/MED/22403682
- Web of Science
- 000303017700079
- Scopus
- 84857765412
- Mendeley
- http://www.mendeley.com/research/modified-method-whole-exome-resequencing-minimal-amounts-starting-dna
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Mendeley | Further Information
{"title"=>"A modified method for whole exome resequencing from minimal amounts of starting DNA", "type"=>"journal", "authors"=>[{"first_name"=>"Iwanka", "last_name"=>"Kozarewa", "scopus_author_id"=>"14048845400"}, {"first_name"=>"Juan Manuel", "last_name"=>"Rosa-Rosa", "scopus_author_id"=>"14424466500"}, {"first_name"=>"Christopher P.", "last_name"=>"Wardell", "scopus_author_id"=>"6506097122"}, {"first_name"=>"Brian A.", "last_name"=>"Walker", "scopus_author_id"=>"56384903600"}, {"first_name"=>"Kerry", "last_name"=>"Fenwick", "scopus_author_id"=>"8690638400"}, {"first_name"=>"Ioannis", "last_name"=>"Assiotis", "scopus_author_id"=>"42260943100"}, {"first_name"=>"Costas", "last_name"=>"Mitsopoulos", "scopus_author_id"=>"6508208267"}, {"first_name"=>"Marketa", "last_name"=>"Zvelebil", "scopus_author_id"=>"7004486825"}, {"first_name"=>"Gareth J.", "last_name"=>"Morgan", "scopus_author_id"=>"56814258400"}, {"first_name"=>"Alan", "last_name"=>"Ashworth", "scopus_author_id"=>"35379025100"}, {"first_name"=>"Christopher J.", "last_name"=>"Lord", "scopus_author_id"=>"7101632397"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84857765412", "sgr"=>"84857765412", "pui"=>"364370636", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"22403682", "doi"=>"10.1371/journal.pone.0032617"}, "id"=>"e1440b7d-b5d4-3338-b966-59ed41d92f49", "abstract"=>"Next generation DNA sequencing (NGS) technologies have revolutionized the pace at which whole genome and exome sequences can be generated. However, despite these advances, many of the methods for targeted resequencing, such as the generation of high-depth exome sequences, are somewhat limited by the relatively large amounts of starting DNA that are normally required. In the case of tumour analysis this is particularly pertinent as many tumour biopsies often return submicrogram quantities of DNA, especially when tumours are microdissected prior to analysis. Here, we present a method for exome capture and resequencing using as little as 50 ng of starting DNA. The sequencing libraries generated by this minimal starting amount (MSA-Cap) method generate datasets that are comparable to standard amount (SA) whole exome libraries that use three micrograms of starting DNA. This method, which can be performed in most laboratories using commonly available reagents, has the potential to enhance large scale profiling efforts such as the resequencing of tumour exomes.", "link"=>"http://www.mendeley.com/research/modified-method-whole-exome-resequencing-minimal-amounts-starting-dna", "reader_count"=>85, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>30, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>18, "Student > Postgraduate"=>2, "Student > Master"=>4, "Other"=>15, "Student > Bachelor"=>2, "Lecturer"=>3, "Lecturer > Senior Lecturer"=>1, "Professor"=>5}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>30, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>18, "Student > Postgraduate"=>2, "Student > Master"=>4, "Other"=>15, "Student > Bachelor"=>2, "Lecturer"=>3, "Lecturer > Senior Lecturer"=>1, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>16, "Agricultural and Biological Sciences"=>46, "Medicine and Dentistry"=>8, "Physics and Astronomy"=>1, "Social Sciences"=>1, "Computer Science"=>6, "Immunology and Microbiology"=>1, "Economics, Econometrics and Finance"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>46}, "Computer Science"=>{"Computer Science"=>6}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>16}, "Unspecified"=>{"Unspecified"=>4}}, "reader_count_by_country"=>{"Sweden"=>2, "United States"=>2, "Brazil"=>1, "United Kingdom"=>4, "Italy"=>1, "Germany"=>1}, "group_count"=>4}CrossRef
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Scopus | Further Information
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PMC Usage Stats | Further Information
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- {"unique-ip"=>"20", "full-text"=>"23", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
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- {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
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Relative Metric
{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[316, 541, 663, 766, 856, 950, 1041, 1128, 1218, 1302, 1382, 1456, 1526, 1593, 1657, 1729, 1796, 1862, 1930, 1999, 2065, 2132, 2202, 2261, 2319]}, {"subject_area"=>"/Biology and life sciences/Computational biology", "average_usage"=>[375, 629, 760, 889, 1000, 1110, 1203, 1298, 1399, 1492, 1603, 1699, 1774, 1855, 1918, 1998, 2062, 2152, 2227, 2312, 2378, 2461, 2528, 2600, 2664]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[324, 551, 679, 782, 879, 971, 1064, 1154, 1245, 1328, 1406, 1476, 1541, 1617, 1683, 1754, 1818, 1893, 1962, 2025, 2092, 2164, 2227, 2294, 2355]}]}Loading … 

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