MicroRNA159 Can Act as a Switch or Tuning MicroRNA Independently of Its Abundance in Arabidopsis
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Mendeley | Further Information

{"title"=>"MicroRNA159 Can Act as a Switch or Tuning MicroRNA Independently of Its Abundance in Arabidopsis", "type"=>"journal", "authors"=>[{"first_name"=>"Maria M.", "last_name"=>"Alonso-Peral"}, {"first_name"=>"Cheng", "last_name"=>"Sun"}, {"first_name"=>"Anthony A.", "last_name"=>"Millar"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"22511963", "sgr"=>"84869825304", "pui"=>"366360175", "scopus"=>"2-s2.0-84869825304", "doi"=>"10.1371/journal.pone.0034751", "issn"=>"1932-6203"}, "id"=>"80c6885f-9586-3bed-a60b-906b1d248478", "abstract"=>"The efficacy of gene silencing by plant microRNAs (miRNAs) is generally assumed to be predominantly determined by their abundance. In Arabidopsis the highly abundant miRNA, miR159, acts as a molecular \"switch\" in vegetative tissues completely silencing the expression of two GAMYB-like genes, MYB33 and MYB65. Here, we show that miR159 has a diminished silencing efficacy in the seed. Using reporter gene constructs, we determined that MIR159 and MYB33 are co-transcribed in the aleurone and embryo of germinating seeds. However in contrast to vegetative tissues, MYB33 is not completely silenced. Instead, miR159 appears to shape the spatio-temporal expression pattern of MYB33 during seed germination. Transcript profiling in a time course during seed germination in wild-type and a mir159 mutant in which miR159 is almost absent, revealed that transcript levels of the GAMYB-like genes were similar between these two genotypes during germination, but much higher in the mir159 mutant once germination had completed. This attenuation in the silencing of the GAMYB-like genes was not explained by a decrease in mature miR159 levels, which remained constant at all time points during seed germination. We propose that miR159 acts as a tuner of GAMYB-like levels in Arabidopsis germinating seeds and that the activity of this miRNA is attenuated in the seed compared to vegetative tissues. This implies that the efficacy of miRNA-mediated silencing is not solely determined by miRNA abundance and target transcript levels, but is being determined through additional mechanisms.", "link"=>"http://www.mendeley.com/research/microrna159-act-switch-tuning-microrna-independently-abundance-arabidopsis", "reader_count"=>60, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>4, "Researcher"=>18, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>2, "Student > Master"=>5, "Other"=>1, "Student > Bachelor"=>12, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>4, "Researcher"=>18, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>2, "Student > Master"=>5, "Other"=>1, "Student > Bachelor"=>12, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>15, "Agricultural and Biological Sciences"=>44, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>44}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>15}}, "reader_count_by_country"=>{"Canada"=>1, "Australia"=>1, "Portugal"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/654963"], "description"=>"<p>(A) Relative <i>MYB33</i> expression levels were measured by quantitative RT-PCR in Shoot Apical Regions (SAR) of 15-day-old plants, 32-day-old rosettes and germinating seeds 5, 24 and 30 h after imbibition, and normalized to <i>CYCLOPHYLIN5</i>. Levels in wild-type SAR were then set to one and the rest of measurements were normalized to this tissue. Black bars correspond to Col-0 and red bars to <i>mir159ab</i> tissues. (B) Mature miR159a (black bars) and miR159b (red bars) levels in the same SAR, rosettes and 24 h-germinating seeds of Col-0 as measured by TaqMan microRNA assays. MicroRNA levels are relative to the small RNA <i>sno101</i>. Error bars represent standard deviation.</p>", "links"=>[], "tags"=>["mrna", "mature", "mir159", "levels", "germinating"], "article_id"=>325456, "categories"=>["Molecular Biology", "Biological Sciences", "Plant Biology"], "users"=>["Maria M. Alonso-Peral", "Cheng Sun", "Anthony A. Millar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034751.g001", "stats"=>{"downloads"=>2, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MYB33_mRNA_and_mature_miR159_levels_are_high_in_germinating_seeds_/325456", "title"=>"<i>MYB33</i> mRNA and mature miR159 levels are high in germinating seeds.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-12 01:30:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/655183"], "description"=>"<p>Dissected aleurone layers of stained <i>mMYB33-GUS</i> (A, D, G), <i>miR159b-GUS</i> (B, E, H) and <i>MYB33-GUS</i> (C, F, I) seeds. Seeds were stratified and kept in the growth chamber for 16, 24 and 32 h prior to staining. Arrowheads point to the part of the aleurone in contact with the radicle tip.</p>", "links"=>[], "tags"=>["aleurone", "layers"], "article_id"=>325680, "categories"=>["Molecular Biology", "Biological Sciences", "Plant Biology"], "users"=>["Maria M. Alonso-Peral", "Cheng Sun", "Anthony A. Millar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034751.g003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Isolated_aleurone_layers_of_mMYB33_GUS_miR159b_GUS_and_MYB33_GUS_during_seed_germination_/325680", "title"=>"Isolated aleurone layers of <i>mMYB33-GUS</i>, <i>miR159b-GUS</i> and <i>MYB33-GUS</i> during seed germination.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-12 01:34:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/655055"], "description"=>"<p>Expression pattern of <i>mMYB33-GUS</i> (A, D, G, J, M, P, S, V, Y), <i>miR159b-GUS</i> (B, E, H, K, N, Q, T, W, Z) and <i>MYB33-GUS</i> (C, F, I, L, O, R, U, X, AB) in the aleurone (A, B, C, G, H, I, M, N, O, S, T, U, Y, Z, AB) and in the embryo (D, E, F, J, K, L, P, Q, R, V, W, X, Y, Z, AB) of germinating seeds 5, 16, 24, 32 and 48 h after imbibition. Seed coat was removed to uncover the aleurone and seeds are oriented so the radicle is at the bottom of the picture. Scale bar for all the aleurones and all the embryos is the same as in A and D respectively, except for Y, Z and AB in which the scale bars is the same as in Y.</p>", "links"=>[], "tags"=>["plant biology", "molecular biology", "Computational biology"], "article_id"=>325550, "categories"=>["Molecular Biology", "Biological Sciences", "Plant Biology"], "users"=>["Maria M. Alonso-Peral", "Cheng Sun", "Anthony A. Millar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034751.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_pattern_of_mMYB33_GUS_miR159b_GUS_and_MYB33_GUS_during_seed_germination_/325550", "title"=>"Expression pattern of <i>mMYB33-GUS</i>, <i>miR159b-GUS</i> and <i>MYB33-GUS</i> during seed germination.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-12 01:32:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/655278"], "description"=>"<p>Relative mRNA levels of <i>MYB33</i> (A), <i>MYB65</i> (B), <i>MYB101</i> (C) and <i>CP1</i> (D) in germinating Col-0 (black bars) and <i>mir159ab</i> (white bars) seeds. Measurements were carried out by quantitative RT-PCR and were normalized to <i>CYCLOPHYLIN5</i>. (E) Germination rate of Col-0 (black bars) and <i>mir159ab</i> (white bars) seeds. (F) Mature miR159 levels in Col-0 germinating seeds as measured by Taqman microRNA assays. MiRNA levels are relative to the small RNA <i>sno101</i>. Error bars represent standard deviations.</p>", "links"=>[], "tags"=>["mirna", "profiling"], "article_id"=>325772, "categories"=>["Molecular Biology", "Biological Sciences", "Plant Biology"], "users"=>["Maria M. Alonso-Peral", "Cheng Sun", "Anthony A. Millar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034751.g004", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcript_and_miRNA_profiling_during_seed_germination_/325772", "title"=>"Transcript and miRNA profiling during seed germination.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-04-12 01:36:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/336184"], "description"=>"<div><p>The efficacy of gene silencing by plant microRNAs (miRNAs) is generally assumed to be predominantly determined by their abundance. In Arabidopsis the highly abundant miRNA, miR159, acts as a molecular “switch” in vegetative tissues completely silencing the expression of two <em>GAMYB-like</em> genes, <em>MYB33</em> and <em>MYB65</em>. Here, we show that miR159 has a diminished silencing efficacy in the seed. Using reporter gene constructs, we determined that <em>MIR159</em> and <em>MYB33</em> are co-transcribed in the aleurone and embryo of germinating seeds. However in contrast to vegetative tissues, <em>MYB33</em> is not completely silenced. Instead, miR159 appears to shape the spatio-temporal expression pattern of MYB33 during seed germination. Transcript profiling in a time course during seed germination in wild-type and a <em>mir159</em> mutant in which miR159 is almost absent, revealed that transcript levels of the <em>GAMYB-like</em> genes were similar between these two genotypes during germination, but much higher in the <em>mir159</em> mutant once germination had completed. This attenuation in the silencing of the <em>GAMYB-like</em> genes was not explained by a decrease in mature miR159 levels, which remained constant at all time points during seed germination. We propose that miR159 acts as a tuner of <em>GAMYB-like</em> levels in Arabidopsis germinating seeds and that the activity of this miRNA is attenuated in the seed compared to vegetative tissues. This implies that the efficacy of miRNA-mediated silencing is not solely determined by miRNA abundance and target transcript levels, but is being determined through additional mechanisms.</p> </div>", "links"=>[], "tags"=>["microrna159", "tuning", "microrna", "independently", "abundance", "arabidopsis"], "article_id"=>126433, "categories"=>["Molecular Biology", "Biological Sciences", "Cell Biology"], "users"=>["Maria M. Alonso-Peral", "Cheng Sun", "Anthony A. Millar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0034751", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/MicroRNA159_Can_Act_as_a_Switch_or_Tuning_MicroRNA_Independently_of_Its_Abundance_in_Arabidopsis/126433", "title"=>"MicroRNA159 Can Act as a Switch or Tuning MicroRNA Independently of Its Abundance in Arabidopsis", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-04-12 01:47:13"}

PMC Usage Stats | Further Information

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Relative Metric

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