Leu1283.43 (L128) and Val2476.40 (V247) of CXCR1 Are Critical Amino Acid Residues for G Protein Coupling and Receptor Activation
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Leu1283.43 (L128) and Val2476.40 (V247) of CXCR1 are critical amino acid residues for G protein coupling and receptor activation", "type"=>"journal", "authors"=>[{"first_name"=>"Xinbing", "last_name"=>"Han", "scopus_author_id"=>"7402400790"}, {"first_name"=>"Souvenir D.", "last_name"=>"Tachado", "scopus_author_id"=>"6701601855"}, {"first_name"=>"Henry", "last_name"=>"Koziel", "scopus_author_id"=>"7003938658"}, {"first_name"=>"William A.", "last_name"=>"Boisvert", "scopus_author_id"=>"6701540944"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"365489866", "scopus"=>"2-s2.0-84865298180", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"22936990", "sgr"=>"84865298180", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0042765"}, "id"=>"0798691d-e696-35e5-8bdb-46beab8d76f0", "abstract"=>"CXCR1, a classic GPCR that binds IL-8, plays a key role in neutrophil activation and migration by activating phospholipase C (PLC)β through Gα(15) and Gα(i) which generates diacylglycerol and inositol phosphates (IPs). In this study, two conserved amino acid residues of CXCR1 on the transmembrane domain (TM) 3 and TM6, Leu128(3.43) (L128) and Val247(6.40) (V247), respectively, were selectively substituted with other amino acids to investigate the role of these conserved residues in CXCR1 activation. Although two selective mutants on Leu128, Leu128Ala (L128A) and Leu128Arg (L128R), demonstrated high binding affinity to IL-8, they were not capable of coupling to G proteins and consequently lost the functional response of the receptors. By contrast, among the four mutants at residue Val247 (TM6.40), replacing Val247 with Ala (V247A) and Asn (V247N) led to constitutive activation of mutant receptors when cotransfected with Gα(15). The V247N mutant also constitutively activated the Gα(i) protein. These results indicate that L128 on TM3.43 is involved in G protein coupling and receptor activation but is unimportant for ligand binding. On the other hand, V247 on TM6.40 plays a critical role in maintaining the receptor in the inactive state, and the substitution of V247 impaired the receptor constraint and stabilized an active conformation. Functionally, there was an increase in chemotaxis in response to IL-8 in cells expressing V247A and V247N. Our findings indicate that Leu128(3.43) and Val247(6.40) are critical for G protein coupling and activation of signaling effectors, providing a valuable insight into the mechanism of CXCR1 activation.", "link"=>"http://www.mendeley.com/research/leu128343-l128-val247640-v247-cxcr1-critical-amino-acid-residues-g-protein-coupling-receptor-activat", "reader_count"=>15, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>7, "Medicine and Dentistry"=>2, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United Kingdom"=>1, "Spain"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84865298180"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84865298180?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865298180&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84865298180&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84865298180", "dc:identifier"=>"SCOPUS_ID:84865298180", "eid"=>"2-s2.0-84865298180", "dc:title"=>"Leu128<sup>3.43</sup> (L128) and Val247<sup>6.40</sup> (V247) of CXCR1 are critical amino acid residues for G protein coupling and receptor activation", "dc:creator"=>"Han X.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-08-24", "prism:coverDisplayDate"=>"24 August 2012", "prism:doi"=>"10.1371/journal.pone.0042765", "citedby-count"=>"12", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Brigham and Women's Hospital", "affiliation-city"=>"Boston", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"Harvard Medical School", "affiliation-city"=>"Boston", "affiliation-country"=>"United States"}], "pubmed-id"=>"22936990", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e42765", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0042765", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2012", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"195"}
  • {"month"=>"9", "year"=>"2012", "pdf_views"=>"40", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"10", "year"=>"2012", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"11", "year"=>"2012", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2012", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"60"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"26"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"44", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/587180"], "description"=>"<p>(A) Basal and IL-8-stimulated inositol phosphate (IP) accumulation in COS-7 cells transiently co-transfected with WT or mutant CXCR1 and Gα<sub>15</sub>. IP production was determined in detail as described under “<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#s2\" target=\"_blank\">Materials and Methods</a>”. Data are mean ± SEM of replicate wells from a representative experiments performed in triplicate. **, <i>P</i><0.01, <i>vs</i>. WT+ Gα<sub>15</sub> (in the absence of IL-8). (B) Calcium signaling capacity in COS-7 cells transfected with CXCR1 WT and V247N mutant. The increase in intracellular calcium concentration ([Ca<sup>2+</sup>]<sub>i</sub>) in COS-7 cells transfected with Gα<sub>15</sub> and CXCR1 WT or V247N mutant was measured using BD calcium assay kit. The cells were stimulated with or without IL-8 (40 nM). Values represent the mean (± the SEM) increase of [Ca<sup>2+</sup>]<sub>i</sub> (<i>n</i> = 4). **, <i>P</i><0.01, <i>vs</i>. WT+ Gα<sub>15</sub> (in the absence of IL-8).</p>", "links"=>[], "tags"=>["mutants"], "article_id"=>257670, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.g005", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Constitutively_active_mutants_of_CXCR1_/257670", "title"=>"Constitutively active mutants of CXCR1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 00:25:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/587604"], "description"=>"1<p>B<sub>max</sub> and K<sub>d</sub> value were estimated from COS-7 cells ligand binding assay as described under “<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#s2\" target=\"_blank\">Materials and Methods</a>”. All assays were done in triplicates. Values are mean ± SEM from 3 experiments.</p>", "links"=>[], "tags"=>["ligand", "binding", "assay", "cxcr1", "wt"], "article_id"=>258088, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.t001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_ligand_binding_assay_of_CXCR1_WT_and_mutants_1_/258088", "title"=>"Summary of ligand binding assay of CXCR1 WT and mutants<sup>1</sup>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-02-20 00:27:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/587389"], "description"=>"<p>(A and B) Surface expression of CXCR1 WT and V247N on COS-7 cells (A) and TSA-201 cells (B). Cells were incubated with mouse anti human CD128a (CXCR1) antibody at 4°C overnight. After washing three times, the cells were incubated with the secondary antibody (DyLight 549-conjugated goat anti-mouse IgG(H+L)) at RT for 1 hr and counterstained with Dapi. Expression of CXCR1 or the mutants was observed using a confocal microscope. The pink color represents the surface expression of CXCR1 WT or mutant with Dapi shown in blue. (C) Inhibition of forskolin-stimulated cAMP production in COS-7 cells. COS-7 cells were transfected with CXCR1 WT or V247N mutant, as well as Gα<sub>i2</sub>, Gβ<sub>1</sub>, and Gγ<sub>2</sub> expression vector. IL-8 significantly inhibited forskolin-induced stimulation of cAMP formation in transfected cells whereas V247N mutation inhibited forskolin-induced cAMP levels even in the absence of IL-8. Transfected cells were used for determination of cAMP levels in the absence <i>(open bars)</i> or in the presence of IL-8 <i>(closed bars)</i>. The results shown are representative. (D) Inhibition of hCG-stimulated cAMP accumulation by V247N mutant. TSA-201 cells were transfected with CXCR1 WT or V247N mutant, as well as LH receptor expression vector. The TSA-201 cells were pretreated with 0.5 mM IBMX for 30 min followed by treatment with hCG (10<sup>−7</sup> M) in the presence or absence of IL-8 (40 nM) for another 30 min. Following incubation with hCG in the presence or absence of IL-8, cells were lysed and intracellular cAMP measured using a RIA kit as described in “Experimental Procedures”. Data presented are the mean ± SEM for three assays with each condition performed in triplicate.</p>", "links"=>[], "tags"=>["mutant", "constitutively", "activates"], "article_id"=>257877, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.g007", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_V247N_mutant_constitutively_activates_G_i_/257877", "title"=>"V247N mutant constitutively activates Gα<sub>i</sub>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 00:26:29"}
  • {"files"=>["https://ndownloader.figshare.com/files/586963"], "description"=>"<p>The CXCR1 mutants were transiently transfected in COS-7 cells. Maximal binding of <sup>125</sup>I-IL-8 to CXCR1 WT and mutants (A) and competition binding experiments (B and C) were performed as described under “<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#s2\" target=\"_blank\">Materials and Methods</a>”. Competitive binding studies were conducted using <sup>125</sup>I-labeled IL-8 and various unlabelled ligands as described under “<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#s2\" target=\"_blank\">Materials and Methods</a>”. Representative maximal binding experiments for L128 and V247 mutants are shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#pone-0042765-g003\" target=\"_blank\">Fig. 3A</a>, performed in triplicates. Mock was transfected with pSG5 plasmid. Representative competitive binding experiments for L128 and V247 mutants are shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#pone-0042765-g003\" target=\"_blank\">Fig. 3(B)</a> and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#pone-0042765-g003\" target=\"_blank\">Fig. 3(C)</a>, performed in triplicates. Nonspecific binding was determined by adding 250 nM unlabeled IL-8. Curve fitting was done using GraphPad Prism data analysis program, and the affinity constant (K<sub>d</sub>) for CXCR1 mutants is shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#pone-0042765-t001\" target=\"_blank\">Table 1</a>.</p>", "links"=>[], "tags"=>["il-8", "cos-7", "cells", "expressing", "wt", "cxcr1"], "article_id"=>257446, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.g003", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Binding_of_125_I_labeled_IL_8_to_COS_7_cells_expressing_WT_CXCR1_or_its_mutants_/257446", "title"=>"Binding of <sup>125</sup>I-labeled IL-8 to COS-7 cells expressing WT CXCR1 or its mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 00:24:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/587514"], "description"=>"<p>Chemotaxis assays were performed as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042765#s2\" target=\"_blank\">Materials and Methods</a>, using HEK293 cells expressing constitutively active mutants (V247A and V247N) or the WT CXCR1. Data presented are the mean ± SEM for three assays with each condition performed in triplicate. *, <i>P</i><0.05, V247A <i>vs.</i> WT (+IL-8). **, <i>P</i><0.01, V247N <i>vs.</i> WT (+IL-8).</p>", "links"=>[], "tags"=>["assay", "cxcr1", "wt", "constitutively"], "article_id"=>258001, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.g008", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chemotaxis_assay_of_CXCR1_WT_and_its_constitutively_active_mutants_/258001", "title"=>"Chemotaxis assay of CXCR1 WT and its constitutively active mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 00:27:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/587060"], "description"=>"<p>(A) Activation of PLCβ<sub>2</sub> by CXCR1 through Gα<sub>15</sub> proteins. COS-7 cells were cotransfected with equal amounts of cDNA (0.3 µg per well per component) encoding either pSG5, PLCβ<sub>2</sub>, Gα<sub>15</sub>, CXCR1 wild type (WT), Gα<sub>15</sub> plus WT, or PLCβ<sub>2</sub> plus WT. Inositol phosphates were measured 1 hours after treatment in the presence (shaded bars) or absence (open bars) of IL-8 (40 nM). Data are mean ± SEM of replicate wells. ** <i>P</i><0.01, Gα<sub>15</sub>+WT (+IL-8) <i>vs</i>. Gα<sub>15</sub>+WT (−IL-8). (B) CXCR1 mutants coupled to Gα<sub>15</sub>. Basal and IL-8-stimulated inositol phosphate (IP) accumulation in COS-7 cells transiently co-transfected with WT (0.3 µg) or mutant CXCR1 (0.3 µg) and Gα<sub>15</sub> (0.3 µg). Data for mutants are summarized from 3–7 experiments, each performed in triplicate, and are expressed as a percentage of WT CXCR1 baseline determined in parallel. The results are mean ± SEM. ** <i>P</i><0.01, <i>vs</i>. WT+ Gα<sub>15</sub> (in the absence of IL-8).</p>", "links"=>[], "tags"=>["mutants", "coupled"], "article_id"=>257552, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.g004", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CXCR1_mutants_coupled_to_G_15_/257552", "title"=>"CXCR1 mutants coupled to Gα<sub>15</sub>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 00:24:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/587260"], "description"=>"<p>(A) COS-7 cells were cotransfected with equal amounts of cDNA (0.1 µg per well per component) encoding Gα<sub>i2</sub>, Gβ<sub>1</sub>, Gγ<sub>2</sub>, PLCβ<sub>2</sub>, the WT CXCR1 or its mutants. (B) COS-7 cells were cotransfected with equal amounts of encoding Gα<sub>qi5</sub> (qi5) and the WT CXCR1 or its mutants. The release of inositol phosphates, induced by 40 nM IL-8, was measured 1 hour after the treatment in the presence or absence of pertussis toxin (PTX) (100 ng/ml) for 18 hrs. Data are mean ± SEM of replicate wells from a representative experiment. **, <i>P</i><0.01, <i>vs</i>. WT+ qi5 (in the absence of IL-8).</p>", "links"=>[], "tags"=>["mutants", "coupled"], "article_id"=>257751, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CXCR1_and_mutants_coupled_to_G_i_/257751", "title"=>"CXCR1 and mutants coupled to Gα<sub>i</sub>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 00:25:48"}
  • {"files"=>["https://ndownloader.figshare.com/files/586751"], "description"=>"<p>The positions of the residues (Leucine 128 and Valine 247) that were targeted for mutagenesis in TM3 and TM6 of the CXCR1 are indicated by gray and black filled circles respectively. The putative disulfide bridge, formed by Cys110/Cys 187 is indicated by “S-S”.</p>", "links"=>[], "tags"=>["diagram", "cxcr1"], "article_id"=>257240, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.g001", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Two_dimensional_diagram_of_human_CXCR1_receptor_/257240", "title"=>"Two-dimensional diagram of human CXCR1 receptor.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 00:22:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/586853"], "description"=>"<p>HEK 293 cells were transiently transfected with CXCR1 or its mutants. Cells were incubated with FITC-conjugated mouse anti-human CD181 (CXCR1) antibody. Specificity of signal was confirmed by staining the cells with mouse IgG1 isotype control. (A) Transfected HEK 293 cell population stained positive for this anti-CXCR1 antibody (M2 region). (B) Fluorescence of positively stained cells was quantified by FACS (± SEM, with CXCR1 WT set to 100%).</p>", "links"=>[], "tags"=>["cxcr1", "wt"], "article_id"=>257345, "categories"=>["Biological Sciences", "Biochemistry", "Cell Biology", "Immunology"], "users"=>["Xinbing Han", "Souvenir D. Tachado", "Henry Koziel", "William A. Boisvert"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0042765.g002", "stats"=>{"downloads"=>2, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FACS_analysis_of_CXCR1_WT_and_mutants_/257345", "title"=>"FACS analysis of CXCR1 WT and mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-02-20 00:23:28"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"24", "full-text"=>"15", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"9"}
  • {"unique-ip"=>"24", "full-text"=>"22", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"3", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2012", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"4", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"3", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[322, 550, 671, 773, 864, 955, 1048, 1135, 1223, 1308, 1387, 1465, 1534, 1602, 1673, 1744, 1813, 1885, 1955, 2026, 2093, 2160, 2228, 2290, 2349]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[319, 556, 679, 785, 881, 970, 1062, 1149, 1236, 1323, 1402, 1474, 1545, 1617, 1681, 1754, 1822, 1892, 1963, 2031, 2099, 2165, 2233, 2299, 2359]}, {"subject_area"=>"/Biology and life sciences/Immunology", "average_usage"=>[312, 555, 680, 785, 880, 965, 1057, 1142, 1230, 1312, 1394, 1471, 1547, 1613, 1681, 1743, 1805, 1871, 1941, 2010, 2071, 2128, 2202, 2266, 2323]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[324, 551, 679, 782, 879, 971, 1064, 1154, 1245, 1328, 1406, 1476, 1541, 1617, 1683, 1754, 1818, 1893, 1962, 2025, 2092, 2164, 2227, 2294, 2355]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[312, 547, 668, 769, 861, 953, 1046, 1135, 1224, 1307, 1388, 1464, 1536, 1606, 1676, 1744, 1812, 1882, 1954, 2020, 2089, 2155, 2218, 2282, 2344]}, {"subject_area"=>"/Medicine and health sciences/Immunology", "average_usage"=>[307, 545, 668, 765, 855, 943, 1029, 1113, 1202, 1285, 1361, 1439, 1506, 1571, 1636, 1706, 1772, 1837, 1901, 1969, 2031, 2096, 2160, 2228, 2287]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[302, 508, 622, 720, 804, 888, 973, 1054, 1141, 1219, 1299, 1370, 1442, 1511, 1574, 1644, 1711, 1782, 1846, 1911, 1971, 2030, 2097, 2155, 2217]}]}
Loading … Spinner
There are currently no alerts