Cell Division Site Placement and Asymmetric Growth in Mycobacteria
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{"title"=>"Cell division site placement and asymmetric growth in Mycobacteria", "type"=>"journal", "authors"=>[{"first_name"=>"Graham", "last_name"=>"Joyce", "scopus_author_id"=>"36604634900"}, {"first_name"=>"Kerstin J.", "last_name"=>"Williams", "scopus_author_id"=>"7404142197"}, {"first_name"=>"Matthew", "last_name"=>"Robb", "scopus_author_id"=>"55868038100"}, {"first_name"=>"Elke", "last_name"=>"Noens", "scopus_author_id"=>"9335713900"}, {"first_name"=>"Barbara", "last_name"=>"Tizzano", "scopus_author_id"=>"8310023700"}, {"first_name"=>"Vahid", "last_name"=>"Shahrezaei", "scopus_author_id"=>"8906131800"}, {"first_name"=>"Brian D.", "last_name"=>"Robertson", "scopus_author_id"=>"7201834614"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84874668909", "doi"=>"10.1371/journal.pone.0044582", "pui"=>"600169160", "pmid"=>"22970255", "scopus"=>"2-s2.0-84874668909", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\n1932-6203 (Linking)"}, "id"=>"d4cf8419-446c-3ba7-9b8e-f354ae4afa82", "abstract"=>"Mycobacteria are members of the actinomycetes that grow by tip extension and lack apparent homologues of the known cell division regulators found in other rod-shaped bacteria. Previous work using static microscopy on dividing mycobacteria led to the hypothesis that these cells can grow and divide asymmetrically, and at a wide range of sizes, in contrast to the cell growth and division patterns observed in the model rod-shaped organisms. In this study, we test this hypothesis using live-cell time-lapse imaging of dividing Mycobacterium smegmatis labelled with fluorescent PBP1a, to probe peptidoglycan synthesis and label the cell septum. We demonstrate that the new septum is placed accurately at mid-cell, and that the asymmetric division observed is a result of differential growth from the cell tips, with a more than 2-fold difference in growth rate between fast and slow growing poles. We also show that the division site is not selected at a characteristic cell length, suggesting this is not an important cue during the mycobacterial cell cycle.", "link"=>"http://www.mendeley.com/research/cell-division-site-placement-asymmetric-growth-mycobacteria", "reader_count"=>51, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Researcher"=>11, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>20, "Student > Postgraduate"=>1, "Student > Master"=>6, "Student > Bachelor"=>3, "Lecturer > Senior Lecturer"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Researcher"=>11, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>20, "Student > Postgraduate"=>1, "Student > Master"=>6, "Student > Bachelor"=>3, "Lecturer > Senior Lecturer"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Engineering"=>3, "Biochemistry, Genetics and Molecular Biology"=>8, "Mathematics"=>1, "Agricultural and Biological Sciences"=>32, "Medicine and Dentistry"=>2, "Business, Management and Accounting"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>32}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>8}, "Mathematics"=>{"Mathematics"=>1}}, "reader_count_by_country"=>{"Poland"=>1, "France"=>1, "Chile"=>1}, "group_count"=>3}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/579754"], "description"=>"<p>. (<b>A</b>) <i>M. smegmatis</i> mc<sup>2</sup> 155 cells with two polar and one internal spots of VanBODIPY staining; note variable cell lengths and eccentric internal spot placement. (<b>B</b>) <i>C. glutamicum</i> cells with three spots are of similar size with a centrally located internal spot. Scale bar = 2 µm. (<b>C</b>) Plotting cell length versus frequency for 3-spot cells (n = 148) from all three species of bacteria, shows there is significantly more cell length variability in mycobacterial populations compared to <i>C. glutamicum</i> (p<0.01 Wilcoxon signed-rank test). (<b>D</b>) More than 95% of <i>C. glutamicum</i> cells contain an internal spot within the central 20% of the cell length, compared to only 70% of mycobacteria. Data for 3-spot cells was collected from three independent experiments.</p>", "links"=>[], "tags"=>["mycobacteria", "compared"], "article_id"=>250245, "categories"=>["Microbiology", "Biotechnology", "Cell Biology", "Infectious Diseases"], "users"=>["Graham Joyce", "Kerstin J. Williams", "Matthew Robb", "Elke Noens", "Barbara Tizzano", "Vahid Shahrezaei", "Brian D. Robertson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044582.g001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Internal_spot_placement_and_cell_length_are_more_variable_in_mycobacteria_compared_to_C_glutamicum_/250245", "title"=>"Internal spot placement and cell length are more variable in mycobacteria compared to <i>C. glutamicum</i>", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-10 00:04:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/579865"], "description"=>"<p>(<b>A</b>) Three fields of uninduced <i>M. smegmatis</i> mc<sup>2</sup>155 pMEND-PBP1a-mCherry stained with VanBODIPY display the characteristic polar and septal staining of nascent peptidoglycan (Green spots). (<b>B</b>) Induction of PBP1a-mCherry with 20 ng/ml Tc for 3.5 hr results in strong expression of red PBP1a-mCherry that localizes to the septum and poles, in a pattern similar to VanBODIPY staining (three fields of cells). (<b>C</b>) Expression of PBP1a-mCherry disrupts the localization of VanBODIPY staining, leaving diffuse green staining across the cell. The columns show the green, red and merged images respectively.</p>", "links"=>[], "tags"=>["pbp1a", "vanbodipy"], "article_id"=>250355, "categories"=>["Microbiology", "Biotechnology", "Cell Biology", "Infectious Diseases"], "users"=>["Graham Joyce", "Kerstin J. Williams", "Matthew Robb", "Elke Noens", "Barbara Tizzano", "Vahid Shahrezaei", "Brian D. Robertson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044582.g002", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Co_localization_of_PBP1a_and_VanBODIPY_in_M_smegmatis_/250355", "title"=>"Co-localization of PBP1a and VanBODIPY in <i>M. smegmatis.</i>", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-10 00:05:55"}
  • {"files"=>["https://ndownloader.figshare.com/files/579999"], "description"=>"<p>PBP1a-mCherry expression in <i>M. smegmatis</i> mc<sup>2</sup>155 was induced with 20 ng/ml Tc for 3.5 hr cells and images were captured every 10 mins as described. Panels <b>A</b> and <b>B</b> are time series of images showing diffuse patches of staining at variable locations between the poles (white arrow). These eventually condensed into a central septal spot (white asterisk) around mid-cell. Cell envelope invagination and separation was not seen in the bright field images until 160 minutes after this condensation event (n = 10; yellow arrow). See <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044582#pone.0044582.s001\" target=\"_blank\">File S1</a> and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044582#pone.0044582.s002\" target=\"_blank\">File S2</a> for complete movie sequences. The septal spots of PBP1a-mCherry form within the central 20% of the cell (Panel <b>C</b>; n = 23), without the outliers seen with Vancomycin staining. Panel D shows a control strain expressing mCherry alone. Septal spots are present in cells of various lengths (panels <b>E</b> and <b>F</b>), ranging between 4 and 12 µm (<b>G</b>), showing that cell division occurs at a wide range of cell lengths, indicating it is not a cue for placement of the new septum. See <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044582#pone.0044582.s003\" target=\"_blank\">File S3</a> and <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044582#pone.0044582.s004\" target=\"_blank\">File S4</a> for complete movie sequences.</p>", "links"=>[], "tags"=>["localizes", "centrally", "septa", "independently"], "article_id"=>250484, "categories"=>["Microbiology", "Biotechnology", "Cell Biology", "Infectious Diseases"], "users"=>["Graham Joyce", "Kerstin J. Williams", "Matthew Robb", "Elke Noens", "Barbara Tizzano", "Vahid Shahrezaei", "Brian D. Robertson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0044582.g003", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PBP1a_mCherry_localizes_centrally_at_septa_that_form_future_cell_division_sites_independently_of_cell_length_/250484", "title"=>"PBP1a-mCherry localizes centrally at septa that form future cell division sites, independently of cell length.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-09-10 00:08:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/306461", "https://ndownloader.figshare.com/files/306504", "https://ndownloader.figshare.com/files/306548", "https://ndownloader.figshare.com/files/306598"], "description"=>"<div><p>Mycobacteria are members of the actinomycetes that grow by tip extension and lack apparent homologues of the known cell division regulators found in other rod-shaped bacteria. Previous work using static microscopy on dividing mycobacteria led to the hypothesis that these cells can grow and divide asymmetrically, and at a wide range of sizes, in contrast to the cell growth and division patterns observed in the model rod-shaped organisms. In this study, we test this hypothesis using live-cell time-lapse imaging of dividing <em>Mycobacterium smegmatis</em> labelled with fluorescent PBP1a, to probe peptidoglycan synthesis and label the cell septum. We demonstrate that the new septum is placed accurately at mid-cell, and that the asymmetric division observed is a result of differential growth from the cell tips, with a more than 2-fold difference in growth rate between fast and slow growing poles. We also show that the division site is not selected at a characteristic cell length, suggesting this is not an important cue during the mycobacterial cell cycle.</p> </div>", "links"=>[], "tags"=>["asymmetric", "mycobacteria"], "article_id"=>120465, "categories"=>["Cancer", "Biotechnology", "Cell Biology", "Microbiology"], "users"=>["Graham Joyce", "Kerstin J. Williams", "Matthew Robb", "Elke Noens", "Barbara Tizzano", "Vahid Shahrezaei", "Brian D. Robertson"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0044582.s001", "https://dx.doi.org/10.1371/journal.pone.0044582.s002", "https://dx.doi.org/10.1371/journal.pone.0044582.s003", "https://dx.doi.org/10.1371/journal.pone.0044582.s004"], "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Cell_Division_Site_Placement_and_Asymmetric_Growth_in_Mycobacteria/120465", "title"=>"Cell Division Site Placement and Asymmetric Growth in Mycobacteria", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2012-09-10 00:07:45"}

PMC Usage Stats | Further Information

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Relative Metric

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