Analysis of Proteolytic Processes and Enzymatic Activities in the Generation of Huntingtin N-Terminal Fragments in an HEK293 Cell Model
- Publication Date
- December 07, 2012
- Journal
- PLOS ONE
- Authors
- Andrew T. N. Tebbenkamp, Keith W. Crosby, Zoe B. Siemienski, Hilda H. Brown, et al
- Volume
- 7
- Issue
- 12
- Pages
- e50750
- DOI
- https://dx.plos.org/10.1371/journal.pone.0050750
- Publisher URL
- http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0050750
- PubMed
- http://www.ncbi.nlm.nih.gov/pubmed/23236391
- PubMed Central
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3517621
- Europe PMC
- http://europepmc.org/abstract/MED/23236391
- Web of Science
- 000312064100033
- Scopus
- 84870933532
- Mendeley
- http://www.mendeley.com/research/analysis-proteolytic-processes-enzymatic-activities-generation-huntingtin-nterminal-fragments-hek293
Loading … 

Events
Loading … 

Mendeley | Further Information
{"title"=>"Analysis of Proteolytic Processes and Enzymatic Activities in the Generation of Huntingtin N-Terminal Fragments in an HEK293 Cell Model", "type"=>"journal", "authors"=>[{"first_name"=>"Andrew T.N.", "last_name"=>"Tebbenkamp", "scopus_author_id"=>"16204188200"}, {"first_name"=>"Keith W.", "last_name"=>"Crosby", "scopus_author_id"=>"55520073500"}, {"first_name"=>"Zoe B.", "last_name"=>"Siemienski", "scopus_author_id"=>"55521074100"}, {"first_name"=>"Hilda H.", "last_name"=>"Brown", "scopus_author_id"=>"54782222300"}, {"first_name"=>"Todd E.", "last_name"=>"Golde", "scopus_author_id"=>"7005214095"}, {"first_name"=>"David R.", "last_name"=>"Borchelt", "scopus_author_id"=>"7005319619"}, {"first_name"=>"Xiao Jiang", "last_name"=>"Li", "scopus_author_id"=>"36012660600"}], "year"=>2012, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84870933532", "doi"=>"10.1371/journal.pone.0050750", "pui"=>"366250513", "pmid"=>"23236391", "scopus"=>"2-s2.0-84870933532", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"1bdd3cc3-2a9c-36b5-9245-7b6d83adb309", "abstract"=>"BACKGROUND: N-terminal fragments of mutant huntingtin (htt) that terminate between residues 90-115, termed cleavage product A or 1 (cp-A/1), form intracellular and intranuclear inclusion bodies in the brains of patients with Huntington's disease (HD). These fragments appear to be proteolytic products of the full-length protein. Here, we use an HEK293 cell culture model to investigate huntingtin proteolytic processing; previous studies of these cells have demonstrated cleavage of htt to cp-A/1 like htt fragments.\\n\\nRESULTS: Recombinant N-terminal htt fragments, terminating at residue 171 (also referred to as cp-B/2 like), were efficiently cleaved to produce cp-A/1 whereas fragments representing endogenous caspase, calpain, and metalloproteinase cleavage products, terminating between residues 400-600, were inefficiently cleaved. Using cysteine-labeling techniques and antibody binding mapping, we localized the C-terminus of the cp-A/1 fragments produced by HEK293 cells to sequences minimally limited by cysteine 105 and an antibody epitope composed of residues 115-124. A combination of genetic and pharmacologic approaches to inhibit potential proteases, including γ-secretase and calpain, proved ineffective in preventing production of cp-A/1.\\n\\nCONCLUSIONS: Our findings indicate that HEK293 cells express a protease that is capable of efficiently cleaving cp-B/2 like fragments of htt with normal or expanded glutamine repeats. For reasons that remain unclear, this protease cleaves longer htt fragments, with normal or expanded glutamine expansions, much less efficiently. The protease in HEK293 cells that is capable of generating a cp-A/1 like htt fragment may be a novel protease with a high preference for a cp-B/2-like htt fragment as substrate.", "link"=>"http://www.mendeley.com/research/analysis-proteolytic-processes-enzymatic-activities-generation-huntingtin-nterminal-fragments-hek293", "reader_count"=>26, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>6, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>10, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>6, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>14, "Medicine and Dentistry"=>2, "Neuroscience"=>3, "Physics and Astronomy"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>3}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>14}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}}, "reader_count_by_country"=>{"United States"=>1, "China"=>1, "United Kingdom"=>1, "France"=>1, "Germany"=>1}, "group_count"=>1}CrossRef
- http://doi.org/10.1111/febs.12418
- http://doi.org/10.1016/j.neuropharm.2018.03.009
- http://doi.org/10.1016/j.neuron.2016.02.003
- http://doi.org/10.1152/ajplung.00217.2014
- http://doi.org/10.1116/1.4984009
- http://doi.org/10.3389/fmicb.2019.02622
- http://doi.org/10.1074/jbc.M117.782300
- http://doi.org/10.15252/embj.201490808
- http://doi.org/10.1021/acs.jproteome.6b00991
Scopus | Further Information
{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84870933532"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84870933532?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870933532&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84870933532&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84870933532", "dc:identifier"=>"SCOPUS_ID:84870933532", "eid"=>"2-s2.0-84870933532", "dc:title"=>"Analysis of Proteolytic Processes and Enzymatic Activities in the Generation of Huntingtin N-Terminal Fragments in an HEK293 Cell Model", "dc:creator"=>"Tebbenkamp A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"7", "prism:issueIdentifier"=>"12", "prism:pageRange"=>nil, "prism:coverDate"=>"2012-12-07", "prism:coverDisplayDate"=>"7 December 2012", "prism:doi"=>"10.1371/journal.pone.0050750", "citedby-count"=>"10", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Florida", "affiliation-city"=>"Gainesville", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"University of Florida College of Medicine", "affiliation-city"=>"Gainesville", "affiliation-country"=>"United States"}], "pubmed-id"=>"23236391", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e50750", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true, "freetoread"=>{"value"=>[{"$"=>"all"}, {"$"=>"publisherfullgold"}, {"$"=>"repository"}, {"$"=>"repositoryvor"}, {"$"=>"repositoryam"}]}, "freetoreadLabel"=>{"value"=>[{"$"=>"All Open Access"}, {"$"=>"Gold"}, {"$"=>"Green"}]}}- {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0050750", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}
Counter
- {"month"=>"12", "year"=>"2012", "pdf_views"=>"33", "xml_views"=>"5", "html_views"=>"182"}
- {"month"=>"1", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"129"}
- {"month"=>"2", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"16"}
- {"month"=>"3", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"35"}
- {"month"=>"4", "year"=>"2013", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"50"}
- {"month"=>"5", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"24"}
- {"month"=>"6", "year"=>"2013", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
- {"month"=>"7", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"34"}
- {"month"=>"8", "year"=>"2013", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
- {"month"=>"9", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"35"}
- {"month"=>"10", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"31"}
- {"month"=>"11", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"19"}
- {"month"=>"12", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"25"}
- {"month"=>"1", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"23"}
- {"month"=>"2", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
- {"month"=>"3", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"4", "html_views"=>"14"}
- {"month"=>"4", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"19"}
- {"month"=>"5", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"21"}
- {"month"=>"6", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
- {"month"=>"7", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"47"}
- {"month"=>"8", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"24"}
- {"month"=>"9", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"26"}
- {"month"=>"10", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"30"}
- {"month"=>"11", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"8"}
- {"month"=>"12", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"14"}
- {"month"=>"1", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
- {"month"=>"2", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"17"}
- {"month"=>"3", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
- {"month"=>"4", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
- {"month"=>"5", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"17"}
- {"month"=>"6", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
- {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"20"}
- {"month"=>"8", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"27"}
- {"month"=>"9", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"32"}
- {"month"=>"10", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
- {"month"=>"11", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"10"}
- {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
- {"month"=>"1", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
- {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"24"}
- {"month"=>"3", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"46"}
- {"month"=>"4", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"26"}
- {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
- {"month"=>"6", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"25"}
- {"month"=>"7", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"19"}
- {"month"=>"8", "year"=>"2016", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"29"}
- {"month"=>"9", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"32"}
- {"month"=>"10", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
- {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"28"}
- {"month"=>"12", "year"=>"2016", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"40"}
- {"month"=>"1", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"33"}
- {"month"=>"2", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
- {"month"=>"3", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"51"}
- {"month"=>"4", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"45"}
- {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"62"}
- {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"40"}
- {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"29"}
- {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
- {"month"=>"9", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"27"}
- {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"29"}
- {"month"=>"11", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"57"}
- {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"18"}
- {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
- {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
- {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
- {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
- {"month"=>"5", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
- {"month"=>"6", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
- {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"4", "html_views"=>"11"}
- {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"15"}
- {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
- {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"20"}
- {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
- {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
- {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"17"}
- {"month"=>"2", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"14"}
- {"month"=>"3", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"21"}
- {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
- {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"19"}
- {"month"=>"6", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"28"}
- {"month"=>"7", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"20"}
- {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
- {"month"=>"9", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
- {"month"=>"10", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"19"}
- {"month"=>"11", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"31"}
- {"month"=>"12", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"24"}
- {"month"=>"1", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"25"}
- {"month"=>"2", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"17"}
- {"month"=>"3", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"25"}
- {"month"=>"4", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"42"}
- {"month"=>"5", "year"=>"2020", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"18"}
- {"month"=>"6", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"26"}
- {"month"=>"7", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
- {"month"=>"8", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"37"}
- {"month"=>"9", "year"=>"2020", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"29"}
- {"month"=>"10", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"35"}
- {"month"=>"11", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"11"}
- {"month"=>"12", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
- {"month"=>"1", "year"=>"2021", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"30"}
PMC Usage Stats | Further Information
- {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2012", "month"=>"12"}
- {"unique-ip"=>"28", "full-text"=>"23", "pdf"=>"25", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"26", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
- {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
- {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
- {"unique-ip"=>"22", "full-text"=>"20", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
- {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
- {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"26", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
- {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
- {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
- {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
- {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
- {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
- {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
- {"unique-ip"=>"7", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
- {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
- {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
- {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
- {"unique-ip"=>"13", "full-text"=>"20", "pdf"=>"3", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
- {"unique-ip"=>"9", "full-text"=>"17", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
- {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
- {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
- {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
- {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
- {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
- {"unique-ip"=>"3", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
- {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
- {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
- {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
- {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
- {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
- {"unique-ip"=>"10", "full-text"=>"2", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"2", "year"=>"2014", "month"=>"9"}
- {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
- {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"2"}
- {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"1", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
- {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
- {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"1"}
- {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
- {"unique-ip"=>"7", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
- {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
- {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
- {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
- {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
- {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
- {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
- {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
- {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
- {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
- {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
- {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
- {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
- {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
- {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
- {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
- {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
- {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
- {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
- {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
- {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
- {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
- {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
- {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
- {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
- {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
- {"unique-ip"=>"15", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
- {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
- {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
- {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
- {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
- {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
- {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
- {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
- {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
- {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
- {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
- {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
- {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
- {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
- {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
- {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"19", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
- {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"38", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
- {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
- {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
- {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
- {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
- {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
- {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
- {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}
- {"unique-ip"=>"2", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"11"}
- {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"12"}
Relative Metric
{"start_date"=>"2012-01-01T00:00:00Z", "end_date"=>"2012-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[319, 556, 679, 785, 881, 970, 1062, 1149, 1236, 1323, 1402, 1474, 1545, 1617, 1681, 1754, 1822, 1892, 1963, 2031, 2099, 2165, 2233, 2299, 2359]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[333, 576, 707, 814, 908, 1004, 1104, 1197, 1280, 1370, 1449, 1531, 1603, 1673, 1742, 1817, 1886, 1954, 2025, 2098, 2171, 2234, 2304, 2365, 2431]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[324, 551, 679, 782, 879, 971, 1064, 1154, 1245, 1328, 1406, 1476, 1541, 1617, 1683, 1754, 1818, 1893, 1962, 2025, 2092, 2164, 2227, 2294, 2355]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[302, 508, 622, 720, 804, 888, 973, 1054, 1141, 1219, 1299, 1370, 1442, 1511, 1574, 1644, 1711, 1782, 1846, 1911, 1971, 2030, 2097, 2155, 2217]}]}Loading … 

There are currently no alerts